Restriction map for
pF1KA1031
(Linear) (Six-base) MAPSORT of: KIAA1031.seq Check: 7518 from: 1 to: 2931
KIAA1031.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2103 2931
Size: 2103 828
AccI GT'mk_AC
Cuts at: 0 55 1099 2931
Size: 55 1044 1832
Fragments arranged by size:
1832 1044 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1648 2797 2931
Size: 1648 1149 134
Fragments arranged by size:
1648 1149 134
AflIII A'CryG_T
Cuts at: 0 1529 1844 2354 2408 2931
Size: 1529 315 510 54 523
Fragments arranged by size:
1529 523 510 315 54
AhdI GACnn_n'nnGTC
Cuts at: 0 813 1716 2931
Size: 813 903 1215
Fragments arranged by size:
1215 903 813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 865 2824 2931
Size: 865 1959 107
Fragments arranged by size:
1959 865 107
AlwNI CAG_nnn'CTG
Cuts at: 0 114 165 486 2636 2931
Size: 114 51 321 2150 295
Fragments arranged by size:
2150 321 295 114 51
ApaI G_GGCC'C
Cuts at: 0 1030 2057 2931
Size: 1030 1027 874
Fragments arranged by size:
1030 1027 874
ApaLI G'TGCA_C
Cuts at: 0 1774 2931
Size: 1774 1157
AvaI C'yCGr_G
Cuts at: 0 230 641 1023 1301 2465 2931
Size: 230 411 382 278 1164 466
Fragments arranged by size:
1164 466 411 382 278 230
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 865 898 2931
Size: 865 33 2033
Fragments arranged by size:
2033 865 33
BamHI G'GATC_C
Cuts at: 0 1403 2931
Size: 1403 1528
BanI G'GyrC_C
Cuts at: 0 1552 1702 2881 2931
Size: 1552 150 1179 50
Fragments arranged by size:
1552 1179 150 50
BanII G_rGCy'C
Cuts at: 0 1030 1205 1381 1441 1919 2057 2747 2931
Size: 1030 175 176 60 478 138 690 184
Fragments arranged by size:
1030 690 478 184 176 175 138 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 1967 2601 2931
Size: 1967 634 330
Fragments arranged by size:
1967 634 330
BbvCI CC'TCA_GC
Cuts at: 0 122 2931
Size: 122 2809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 221 2931
Size: 187 34 2710
Fragments arranged by size:
2710 187 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 355 2931
Size: 355 2576
BglI GCCn_nnn'nGGC
Cuts at: 0 839 2931
Size: 839 2092
BglII A'GATC_T
Cuts at: 0 1664 2931
Size: 1664 1267
Bme1580I G_kGCm'C
Cuts at: 0 1030 1555 1778 2057 2821 2931
Size: 1030 525 223 279 764 110
Fragments arranged by size:
1030 764 525 279 223 110
BmgBI CAC'GTC
Cuts at: 0 1530 1845 2615 2931
Size: 1530 315 770 316
Fragments arranged by size:
1530 770 316 315
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 496 2931
Size: 394 102 2435
Fragments arranged by size:
2435 394 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1164 2931
Size: 1164 1767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 921 2121 2462 2668 2931
Size: 325 596 1200 341 206 263
Fragments arranged by size:
1200 596 341 325 263 206
Bpu10I CC'TnA_GC
Cuts at: 0 122 2931
Size: 122 2809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1354 1783 1903 2122 2931
Size: 82 1272 429 120 219 809
Fragments arranged by size:
1272 809 429 219 120 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1060 1135 2931
Size: 1060 75 1796
Fragments arranged by size:
1796 1060 75
BsaAI yAC'GTr
Cuts at: 0 633 2931
Size: 633 2298
BsaHI Gr'CG_yC
Cuts at: 0 390 2627 2931
Size: 390 2237 304
Fragments arranged by size:
2237 390 304
BsaWI w'CCGG_w
Cuts at: 0 1373 2931
Size: 1373 1558
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 2931
Size: 226 30 2675
Fragments arranged by size:
2675 226 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1153 1534 1555 2359 2755 2931
Size: 1153 381 21 804 396 176
Fragments arranged by size:
1153 804 396 381 176 21
BseYI C'CCAG_C
Cuts at: 0 324 651 739 866 1584 2056 2636 2931
Size: 324 327 88 127 718 472 580 295
Fragments arranged by size:
718 580 472 327 324 295 127 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 190 432 1482 2931
Size: 127 63 242 1050 1449
Fragments arranged by size:
1449 1050 242 127 63
BsiEI CG_ry'CG
Cuts at: 0 1368 2298 2931
Size: 1368 930 633
Fragments arranged by size:
1368 930 633
BsiHKAI G_wGCw'C
Cuts at: 0 1179 1340 1381 1637 1778 1832 1919 2040
Size: 1179 161 41 256 141 54 87 121
Cuts at: 2040 2747 2931
Size: 707 184
Fragments arranged by size:
1179 707 256 184 161 141 121 87
54 41
Bsp1286I G_dGCh'C
Cuts at: 0 1030 1179 1205 1340 1381 1441 1555 1637
Size: 1030 149 26 135 41 60 114 82
Cuts at: 1637 1778 1832 1919 2040 2057 2747 2821 2931
Size: 141 54 87 121 17 690 74 110
Fragments arranged by size:
1030 690 149 141 135 121 114 110
87 82 74 60 54 41 26 17
BspEI T'CCGG_A
Cuts at: 0 1373 2931
Size: 1373 1558
BspHI T'CATG_A
Cuts at: 0 2366 2513 2931
Size: 2366 147 418
Fragments arranged by size:
2366 418 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 1058 1577 2078 2103 2931
Size: 713 345 519 501 25 828
Fragments arranged by size:
828 713 519 501 345 25
BsrBI CCG'CTC
Cuts at: 0 1545 1787 2181 2931
Size: 1545 242 394 750
Fragments arranged by size:
1545 750 394 242
BsrDI GCAATG_nn'
Cuts at: 0 135 2931
Size: 135 2796
BsrFI r'CCGG_y
Cuts at: 0 419 1367 2762 2878 2931
Size: 419 948 1395 116 53
Fragments arranged by size:
1395 948 419 116 53
BssSI C'ACGA_G
Cuts at: 0 1103 1255 2931
Size: 1103 152 1676
Fragments arranged by size:
1676 1103 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2699 2931
Size: 2699 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 990 1434 2931
Size: 990 444 1497
Fragments arranged by size:
1497 990 444
BstYI r'GATC_y
Cuts at: 0 1403 1664 1811 2381 2483 2931
Size: 1403 261 147 570 102 448
Fragments arranged by size:
1403 570 448 261 147 102
BtgI C'CryG_G
Cuts at: 0 281 2495 2654 2730 2821 2931
Size: 281 2214 159 76 91 110
Fragments arranged by size:
2214 281 159 110 91 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1599 2931
Size: 1599 1332
BtsI GCAGTG_nn'
Cuts at: 0 557 898 943 1217 2163 2236 2526 2572
Size: 557 341 45 274 946 73 290 46
Cuts at: 2572 2931
Size: 359
Fragments arranged by size:
946 557 359 341 290 274 73 46
45
DrdI GACnn_nn'nnGTC
Cuts at: 0 775 2931
Size: 775 2156
EaeI y'GGCC_r
Cuts at: 0 194 992 1365 2091 2295 2789 2931
Size: 194 798 373 726 204 494 142
Fragments arranged by size:
798 726 494 373 204 194 142
EagI C'GGCC_G
Cuts at: 0 1365 2295 2931
Size: 1365 930 636
Fragments arranged by size:
1365 930 636
EarI CTCTTCn'nnn_
Cuts at: 0 1579 2931
Size: 1579 1352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1804 2931
Size: 37 1767 1127
Fragments arranged by size:
1767 1127 37
EcoICRI GAG'CTC
Cuts at: 0 1379 1917 2745 2931
Size: 1379 538 828 186
Fragments arranged by size:
1379 828 538 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 921 1648 2121 2462 2668 2797 2931
Size: 325 596 727 473 341 206 129 134
Fragments arranged by size:
727 596 473 341 325 206 134 129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1879 2444 2931
Size: 1879 565 487
Fragments arranged by size:
1879 565 487
EcoO109I rG'GnC_Cy
Cuts at: 0 518 731 893 1027 1297 1329 2133 2187
Size: 518 213 162 134 270 32 804 54
Cuts at: 2187 2450 2871 2931
Size: 263 421 60
Fragments arranged by size:
804 518 421 270 263 213 162 134
60 54 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1257 1446 1767 2931
Size: 1257 189 321 1164
Fragments arranged by size:
1257 1164 321 189
HaeII r_GCGC'y
Cuts at: 0 304 325 2931
Size: 304 21 2606
Fragments arranged by size:
2606 304 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 799 831 1702 1734 2931
Size: 748 32 19 32 871 32 1197
Fragments arranged by size:
1197 871 748 32 32 32 19
HincII GTy'rAC
Cuts at: 0 56 1100 2931
Size: 56 1044 1831
Fragments arranged by size:
1831 1044 56
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 2642 2931
Size: 1754 888 289
Fragments arranged by size:
1754 888 289
MscI TGG'CCA
Cuts at: 0 2093 2791 2931
Size: 2093 698 140
Fragments arranged by size:
2093 698 140
MslI CAynn'nnrTG
Cuts at: 0 2515 2931
Size: 2515 416
MspA1I CmG'CkG
Cuts at: 0 845 870 1584 1889 2633 2723 2931
Size: 845 25 714 305 744 90 208
Fragments arranged by size:
845 744 714 305 208 90 25
NaeI GCC'GGC
Cuts at: 0 1369 2931
Size: 1369 1562
NcoI C'CATG_G
Cuts at: 0 281 2654 2730 2931
Size: 281 2373 76 201
Fragments arranged by size:
2373 281 201 76
NgoMIV G'CCGG_C
Cuts at: 0 1367 2931
Size: 1367 1564
NspI r_CATG'y
Cuts at: 0 256 1212 2011 2358 2394 2412 2811 2931
Size: 256 956 799 347 36 18 399 120
Fragments arranged by size:
956 799 399 347 256 120 36 18
PciI A'CATG_T
Cuts at: 0 2354 2408 2931
Size: 2354 54 523
Fragments arranged by size:
2354 523 54
PflMI CCAn_nnn'nTGG
Cuts at: 0 486 528 2313 2636 2931
Size: 486 42 1785 323 295
Fragments arranged by size:
1785 486 323 295 42
PfoI T'CCnGG_A
Cuts at: 0 140 803 1823 2384 2931
Size: 140 663 1020 561 547
Fragments arranged by size:
1020 663 561 547 140
PpuMI rG'GwC_Cy
Cuts at: 0 731 893 2133 2187 2450 2871 2931
Size: 731 162 1240 54 263 421 60
Fragments arranged by size:
1240 731 421 263 162 60 54
PshAI GACnn'nnGTC
Cuts at: 0 1160 2931
Size: 1160 1771
PspOMI G'GGCC_C
Cuts at: 0 1026 2053 2931
Size: 1026 1027 878
Fragments arranged by size:
1027 1026 878
PspXI vC'TCGA_Gb
Cuts at: 0 641 2931
Size: 641 2290
PstI C_TGCA'G
Cuts at: 0 44 1937 2075 2931
Size: 44 1893 138 856
Fragments arranged by size:
1893 856 138 44
PvuII CAG'CTG
Cuts at: 0 870 1889 2931
Size: 870 1019 1042
Fragments arranged by size:
1042 1019 870
SacI G_AGCT'C
Cuts at: 0 1381 1919 2747 2931
Size: 1381 538 828 184
Fragments arranged by size:
1381 828 538 184
SalI G'TCGA_C
Cuts at: 0 54 1098 2931
Size: 54 1044 1833
Fragments arranged by size:
1833 1044 54
SanDI GG'GwC_CC
Cuts at: 0 893 2450 2931
Size: 893 1557 481
Fragments arranged by size:
1557 893 481
SapI GCTCTTCn'nnn_
Cuts at: 0 1579 2931
Size: 1579 1352
ScaI AGT'ACT
Cuts at: 0 1804 1942 1987 2143 2931
Size: 1804 138 45 156 788
Fragments arranged by size:
1804 788 156 138 45
SexAI A'CCwGG_T
Cuts at: 0 215 2931
Size: 215 2716
SfcI C'TryA_G
Cuts at: 0 40 1933 2071 2145 2931
Size: 40 1893 138 74 786
Fragments arranged by size:
1893 786 138 74 40
SmaI CCC'GGG
Cuts at: 0 232 1025 1303 2931
Size: 232 793 278 1628
Fragments arranged by size:
1628 793 278 232
SmlI C'TyrA_G
Cuts at: 0 97 641 1333 1798 1918 2137 2931
Size: 97 544 692 465 120 219 794
Fragments arranged by size:
794 692 544 465 219 120 97
SphI G_CATG'C
Cuts at: 0 1212 2011 2931
Size: 1212 799 920
Fragments arranged by size:
1212 920 799
StuI AGG'CCT
Cuts at: 0 88 403 1270 2931
Size: 88 315 867 1661
Fragments arranged by size:
1661 867 315 88
StyI C'CwwG_G
Cuts at: 0 281 1241 1706 2153 2174 2204 2654 2730
Size: 281 960 465 447 21 30 450 76
Cuts at: 2730 2792 2931
Size: 62 139
Fragments arranged by size:
960 465 450 447 281 139 76 62
30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 1267 1552 2023 2211 2931
Size: 907 360 285 471 188 720
Fragments arranged by size:
907 720 471 360 285 188
TatI w'GTAC_w
Cuts at: 0 173 1802 1940 1985 2141 2931
Size: 173 1629 138 45 156 790
Fragments arranged by size:
1629 790 173 156 138 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 2569 2601 2931
Size: 226 32 2311 32 330
Fragments arranged by size:
2311 330 226 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2028 2931
Size: 2028 903
XhoI C'TCGA_G
Cuts at: 0 641 2931
Size: 641 2290
XmaI C'CCGG_G
Cuts at: 0 230 1023 1301 2931
Size: 230 793 278 1630
Fragments arranged by size:
1630 793 278 230
XmnI GAAnn'nnTTC
Cuts at: 0 2608 2931
Size: 2608 323
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SalI SanDI SapI ScaI SexAI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BbeI BclI BfrBI
BlpI BmtI BsaBI BsiWI BsmI BsmBI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacII SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI