Restriction map for
pF1KA1032
(Linear) (Six-base) MAPSORT of: KIAA1032.seq Check: 4148 from: 1 to: 5109
KIAA1032.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4842 5109
Size: 4842 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 1034 3272 3850 3860 3877 4132 4144
Size: 422 612 2238 578 10 17 255 12
Cuts at: 4144 5109
Size: 965
Fragments arranged by size:
2238 965 612 578 422 255 17 12
10
AfeI AGC'GCT
Cuts at: 0 2794 3249 5109
Size: 2794 455 1860
Fragments arranged by size:
2794 1860 455
AflIII A'CryG_T
Cuts at: 0 62 2339 2747 5109
Size: 62 2277 408 2362
Fragments arranged by size:
2362 2277 408 62
AhdI GACnn_n'nnGTC
Cuts at: 0 1329 2010 3141 5109
Size: 1329 681 1131 1968
Fragments arranged by size:
1968 1329 1131 681
AleI CACnn'nnGTG
Cuts at: 0 67 220 2405 2746 4424 5109
Size: 67 153 2185 341 1678 685
Fragments arranged by size:
2185 1678 685 341 153 67
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 1835 2233 5109
Size: 461 1374 398 2876
Fragments arranged by size:
2876 1374 461 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 3790 3822 5109
Size: 133 32 3625 32 1287
Fragments arranged by size:
3625 1287 133 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1791 3228 3456 4007 4624 5109
Size: 1791 1437 228 551 617 485
Fragments arranged by size:
1791 1437 617 551 485 228
ApaI G_GGCC'C
Cuts at: 0 277 3119 4727 5109
Size: 277 2842 1608 382
Fragments arranged by size:
2842 1608 382 277
ApaLI G'TGCA_C
Cuts at: 0 1647 5109
Size: 1647 3462
ApoI r'AATT_y
Cuts at: 0 55 2175 3189 5109
Size: 55 2120 1014 1920
Fragments arranged by size:
2120 1920 1014 55
AvaI C'yCGr_G
Cuts at: 0 704 1394 1915 2969 3479 3616 4834 5109
Size: 704 690 521 1054 510 137 1218 275
Fragments arranged by size:
1218 1054 704 690 521 510 275 137
BamHI G'GATC_C
Cuts at: 0 236 2083 5109
Size: 236 1847 3026
Fragments arranged by size:
3026 1847 236
BanI G'GyrC_C
Cuts at: 0 43 334 1050 1170 1230 2386 2676 3496
Size: 43 291 716 120 60 1156 290 820
Cuts at: 3496 5092 5109
Size: 1596 17
Fragments arranged by size:
1596 1156 820 716 291 290 120 60
43 17
BanII G_rGCy'C
Cuts at: 0 277 703 868 1106 1834 1931 2965 3119
Size: 277 426 165 238 728 97 1034 154
Cuts at: 3119 3899 4465 4727 4785 5109
Size: 780 566 262 58 324
Fragments arranged by size:
1034 780 728 566 426 324 277 262
238 165 154 97 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 1091 1621 2125 3188 3567 4108 4450 4577
Size: 1091 530 504 1063 379 541 342 127
Cuts at: 4577 5109
Size: 532
Fragments arranged by size:
1091 1063 541 532 530 504 379 342
127
BbvCI CC'TCA_GC
Cuts at: 0 763 5109
Size: 763 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 121 2682 2716 5109
Size: 87 34 2561 34 2393
Fragments arranged by size:
2561 2393 87 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 863 1168 1201 1445 5109
Size: 863 305 33 244 3664
Fragments arranged by size:
3664 863 305 244 33
BglII A'GATC_T
Cuts at: 0 326 1919 1940 5109
Size: 326 1593 21 3169
Fragments arranged by size:
3169 1593 326 21
BlpI GC'TnA_GC
Cuts at: 0 805 826 1100 1241 1259 2323 3898 4732
Size: 805 21 274 141 18 1064 1575 834
Cuts at: 4732 4816 5109
Size: 84 293
Fragments arranged by size:
1575 1064 834 805 293 274 141 84
21 18
Bme1580I G_kGCm'C
Cuts at: 0 48 229 277 1651 2671 3119 3501 3647
Size: 48 181 48 1374 1020 448 382 146
Cuts at: 3647 3755 3985 4727 5109
Size: 108 230 742 382
Fragments arranged by size:
1374 1020 742 448 382 382 230 181
146 108 48 48
BmgBI CAC'GTC
Cuts at: 0 2012 2340 2496 5109
Size: 2012 328 156 2613
Fragments arranged by size:
2613 2012 328 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 1159 4634 5109
Size: 429 730 3475 475
Fragments arranged by size:
3475 730 475 429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 1848 5109
Size: 762 1086 3261
Fragments arranged by size:
3261 1086 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 454 991 1003 1015 1051 1554 2872
Size: 299 155 537 12 12 36 503 1318
Cuts at: 2872 2941 3523 3673 3817 4474 5109
Size: 69 582 150 144 657 635
Fragments arranged by size:
1318 657 635 582 537 503 299 155
150 144 69 36 12 12
Bpu10I CC'TnA_GC
Cuts at: 0 763 3967 5109
Size: 763 3204 1142
Fragments arranged by size:
3204 1142 763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1864 4233 4309 4423 4654 5044 5109
Size: 1864 2369 76 114 231 390 65
Fragments arranged by size:
2369 1864 390 231 114 76 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 1456 5109
Size: 208 1248 3653
Fragments arranged by size:
3653 1248 208
BsaAI yAC'GTr
Cuts at: 0 65 69 5109
Size: 65 4 5040
Fragments arranged by size:
5040 65 4
BsaBI GATnn'nnATC
Cuts at: 0 2035 5109
Size: 2035 3074
BsaHI Gr'CG_yC
Cuts at: 0 1654 2609 5109
Size: 1654 955 2500
Fragments arranged by size:
2500 1654 955
BsaWI w'CCGG_w
Cuts at: 0 1853 2852 5109
Size: 1853 999 2257
Fragments arranged by size:
2257 1853 999
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 880 910 2185 2215 2939 2969
Size: 706 30 144 30 1275 30 724 30
Cuts at: 2969 3691 3706 3721 3736 3790 3820 5059 5089
Size: 722 15 15 15 54 30 1239 30
Cuts at: 5089 5109
Size: 20
Fragments arranged by size:
1275 1239 724 722 706 144 54 30
30 30 30 30 30 20 15 15
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 421 754 988 1012 1015 1018 1036
Size: 283 138 333 234 24 3 3 18
Cuts at: 1036 1039 1048 1051 1054 1057 1060 1291 1603
Size: 3 9 3 3 3 3 231 312
Cuts at: 1603 2185 2440 2643 3313 3484 3676 5098 5109
Size: 582 255 203 670 171 192 1422 11
Fragments arranged by size:
1422 670 582 333 312 283 255 234
231 203 192 171 138 24 18 11
9 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 127 131 508 542 947 1076 2865 3118
Size: 127 4 377 34 405 129 1789 253
Cuts at: 3118 4305 4727 4778 4931 5109
Size: 1187 422 51 153 178
Fragments arranged by size:
1789 1187 422 405 377 253 178 153
129 127 51 34 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 596 1734 2518 3475 5109
Size: 103 493 1138 784 957 1634
Fragments arranged by size:
1634 1138 957 784 493 103
BsiEI CG_ry'CG
Cuts at: 0 2208 2763 4699 4968 5109
Size: 2208 555 1936 269 141
Fragments arranged by size:
2208 1936 555 269 141
BsiHKAI G_wGCw'C
Cuts at: 0 100 854 1651 1834 1880 1931 2695 3305
Size: 100 754 797 183 46 51 764 610
Cuts at: 3305 3686 3899 5109
Size: 381 213 1210
Fragments arranged by size:
1210 797 764 754 610 381 213 183
100 51 46
BsiWI C'GTAC_G
Cuts at: 0 65 5109
Size: 65 5044
BsmI GAATG_Cn'
Cuts at: 0 3758 5109
Size: 3758 1351
Bsp1286I G_dGCh'C
Cuts at: 0 48 100 229 277 703 854 868 1106
Size: 48 52 129 48 426 151 14 238
Cuts at: 1106 1651 1834 1880 1931 2671 2695 2965 3119
Size: 545 183 46 51 740 24 270 154
Cuts at: 3119 3305 3501 3647 3686 3755 3899 3985 4465
Size: 186 196 146 39 69 144 86 480
Cuts at: 4465 4727 4785 5109
Size: 262 58 324
Fragments arranged by size:
740 545 480 426 324 270 262 238
196 186 183 154 151 146 144 129
86 69 58 52 51 48 48 46
39 24 14
BspHI T'CATG_A
Cuts at: 0 2687 3764 5109
Size: 2687 1077 1345
Fragments arranged by size:
2687 1345 1077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1799 4067 4478 4526 4842 5109
Size: 1799 2268 411 48 316 267
Fragments arranged by size:
2268 1799 411 316 267 48
BsrBI CCG'CTC
Cuts at: 0 885 4962 5109
Size: 885 4077 147
Fragments arranged by size:
4077 885 147
BsrDI GCAATG_nn'
Cuts at: 0 3996 5109
Size: 3996 1113
BsrGI T'GTAC_A
Cuts at: 0 3053 4536 5109
Size: 3053 1483 573
Fragments arranged by size:
3053 1483 573
BssHII G'CGCG_C
Cuts at: 0 4879 5109
Size: 4879 230
BssSI C'ACGA_G
Cuts at: 0 1504 1644 1774 5109
Size: 1504 140 130 3335
Fragments arranged by size:
3335 1504 140 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 518 858 1743 1748 1757 1998 4479 5109
Size: 518 340 885 5 9 241 2481 630
Fragments arranged by size:
2481 885 630 518 340 241 9 5
BstBI TT'CG_AA
Cuts at: 0 1667 3809 5109
Size: 1667 2142 1300
Fragments arranged by size:
2142 1667 1300
BstEII G'GTnAC_C
Cuts at: 0 1950 5109
Size: 1950 3159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 1527 2982 4305 4426 5109
Size: 432 1095 1455 1323 121 683
Fragments arranged by size:
1455 1323 1095 683 432 121
BstYI r'GATC_y
Cuts at: 0 236 326 1247 1895 1919 1940 2083 2519
Size: 236 90 921 648 24 21 143 436
Cuts at: 2519 5109
Size: 2590
Fragments arranged by size:
2590 921 648 436 236 143 90 24
21
Bsu36I CC'TnA_GG
Cuts at: 0 1253 5109
Size: 1253 3856
BtgI C'CryG_G
Cuts at: 0 770 2042 3293 3563 3815 4154 5109
Size: 770 1272 1251 270 252 339 955
Fragments arranged by size:
1272 1251 955 770 339 270 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 90 1713 5109
Size: 90 1623 3396
Fragments arranged by size:
3396 1623 90
BtsI GCAGTG_nn'
Cuts at: 0 584 612 4378 5109
Size: 584 28 3766 731
Fragments arranged by size:
3766 731 584 28
DraIII CAC_nnn'GTG
Cuts at: 0 233 1757 2487 5109
Size: 233 1524 730 2622
Fragments arranged by size:
2622 1524 730 233
DrdI GACnn_nn'nnGTC
Cuts at: 0 304 2098 5109
Size: 304 1794 3011
Fragments arranged by size:
3011 1794 304
EaeI y'GGCC_r
Cuts at: 0 1193 1517 1679 3080 4696 4904 4965 5039
Size: 1193 324 162 1401 1616 208 61 74
Cuts at: 5039 5109
Size: 70
Fragments arranged by size:
1616 1401 1193 324 208 162 74 70
61
EagI C'GGCC_G
Cuts at: 0 4696 4965 5109
Size: 4696 269 144
Fragments arranged by size:
4696 269 144
EarI CTCTTCn'nnn_
Cuts at: 0 398 798 959 1822 3932 4453 5109
Size: 398 400 161 863 2110 521 656
Fragments arranged by size:
2110 863 656 521 400 398 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1861 2013 5067 5109
Size: 244 1617 152 3054 42
Fragments arranged by size:
3054 1617 244 152 42
EcoICRI GAG'CTC
Cuts at: 0 1832 1929 3897 5109
Size: 1832 97 1968 1212
Fragments arranged by size:
1968 1832 1212 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 422 454 991 1003 1015 1034 1051
Size: 299 123 32 537 12 12 19 17
Cuts at: 1051 1554 2872 2941 3272 3523 3673 3817 3850
Size: 503 1318 69 331 251 150 144 33
Cuts at: 3850 3860 3877 4132 4144 4474 5109
Size: 10 17 255 12 330 635
Fragments arranged by size:
1318 635 537 503 331 330 299 255
251 150 144 123 69 33 32 19
17 17 12 12 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 3970 5109
Size: 997 2973 1139
Fragments arranged by size:
2973 1139 997
EcoO109I rG'GnC_Cy
Cuts at: 0 273 274 342 978 1161 1182 1788 2892
Size: 273 1 68 636 183 21 606 1104
Cuts at: 2892 3115 4724 4831 5109
Size: 223 1609 107 278
Fragments arranged by size:
1609 1104 636 606 278 273 223 183
107 68 21 1
EcoRI G'AATT_C
Cuts at: 0 3189 5109
Size: 3189 1920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3025 4464 5109
Size: 3025 1439 645
Fragments arranged by size:
3025 1439 645
HaeII r_GCGC'y
Cuts at: 0 2796 3251 3660 4823 4946 5109
Size: 2796 455 409 1163 123 163
Fragments arranged by size:
2796 1163 455 409 163 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 880 912 1315 1347 1996 2028
Size: 147 32 701 32 403 32 649 32
Cuts at: 2028 2233 2265 2884 2916 3127 3159 3249 3281
Size: 205 32 619 32 211 32 90 32
Cuts at: 3281 3460 3492 3562 3594 3691 3706 3723 3738
Size: 179 32 70 32 97 15 17 15
Cuts at: 3738 4459 4491 5059 5091 5109
Size: 721 32 568 32 18
Fragments arranged by size:
721 701 649 619 568 403 211 205
179 147 97 90 70 32 32 32
32 32 32 32 32 32 32 32
32 18 17 15 15
HincII GTy'rAC
Cuts at: 0 4713 5109
Size: 4713 396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 2228 2783 2801 5045 5109
Size: 893 1335 555 18 2244 64
Fragments arranged by size:
2244 1335 893 555 64 18
MscI TGG'CCA
Cuts at: 0 1195 1519 5041 5109
Size: 1195 324 3522 68
Fragments arranged by size:
3522 1195 324 68
MslI CAynn'nnrTG
Cuts at: 0 67 109 220 560 1870 1873 2041 2215
Size: 67 42 111 340 1310 3 168 174
Cuts at: 2215 2311 2405 2407 2746 3763 4424 4978 5109
Size: 96 94 2 339 1017 661 554 131
Fragments arranged by size:
1310 1017 661 554 340 339 174 168
131 111 96 94 67 42 3 2
MspA1I CmG'CkG
Cuts at: 0 131 825 1138 1240 1383 1815 2327 2817
Size: 131 694 313 102 143 432 512 490
Cuts at: 2817 3795 4314 4917 5109
Size: 978 519 603 192
Fragments arranged by size:
978 694 603 519 512 490 432 313
192 143 131 102
NcoI C'CATG_G
Cuts at: 0 3293 3815 4154 5109
Size: 3293 522 339 955
Fragments arranged by size:
3293 955 522 339
NruI TCG'CGA
Cuts at: 0 1732 5109
Size: 1732 3377
NspI r_CATG'y
Cuts at: 0 1473 1746 3547 5109
Size: 1473 273 1801 1562
Fragments arranged by size:
1801 1562 1473 273
PasI CC'CwG_GG
Cuts at: 0 696 1144 1441 2055 2671 4554 5109
Size: 696 448 297 614 616 1883 555
Fragments arranged by size:
1883 696 616 614 555 448 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 3456 4560 4624 5109
Size: 3456 1104 64 485
Fragments arranged by size:
3456 1104 485 64
PfoI T'CCnGG_A
Cuts at: 0 365 992 1537 1898 1934 2807 2888 4451
Size: 365 627 545 361 36 873 81 1563
Cuts at: 4451 4617 5109
Size: 166 492
Fragments arranged by size:
1563 873 627 545 492 365 361 166
81 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2461 2493 3790 3822 5109
Size: 2461 32 1297 32 1287
Fragments arranged by size:
2461 1297 1287 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 342 978 1788 2892 4831 5109
Size: 342 636 810 1104 1939 278
Fragments arranged by size:
1939 1104 810 636 342 278
PshAI GACnn'nnGTC
Cuts at: 0 4829 5109
Size: 4829 280
PspOMI G'GGCC_C
Cuts at: 0 273 3115 4723 5109
Size: 273 2842 1608 386
Fragments arranged by size:
2842 1608 386 273
PspXI vC'TCGA_Gb
Cuts at: 0 3616 5109
Size: 3616 1493
PstI C_TGCA'G
Cuts at: 0 863 1239 1388 1814 4012 4853 4916 5109
Size: 863 376 149 426 2198 841 63 193
Fragments arranged by size:
2198 863 841 426 376 193 149 63
PvuII CAG'CTG
Cuts at: 0 131 825 1138 1240 1383 2817 3795 4314
Size: 131 694 313 102 143 1434 978 519
Cuts at: 4314 4917 5109
Size: 603 192
Fragments arranged by size:
1434 978 694 603 519 313 192 143
131 102
SacI G_AGCT'C
Cuts at: 0 1834 1931 3899 5109
Size: 1834 97 1968 1210
Fragments arranged by size:
1968 1834 1210 97
SanDI GG'GwC_CC
Cuts at: 0 4831 5109
Size: 4831 278
SapI GCTCTTCn'nnn_
Cuts at: 0 959 1822 4453 5109
Size: 959 863 2631 656
Fragments arranged by size:
2631 959 863 656
SbfI CC_TGCA'GG
Cuts at: 0 863 5109
Size: 863 4246
SfcI C'TryA_G
Cuts at: 0 714 859 1235 1384 1810 4008 4849 4912
Size: 714 145 376 149 426 2198 841 63
Cuts at: 4912 5109
Size: 197
Fragments arranged by size:
2198 841 714 426 376 197 149 145
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1201 5109
Size: 1201 3908
SmaI CCC'GGG
Cuts at: 0 706 1396 3481 5109
Size: 706 690 2085 1628
Fragments arranged by size:
2085 1628 706 690
SmlI C'TyrA_G
Cuts at: 0 1879 3616 4212 4324 4438 4669 5059 5109
Size: 1879 1737 596 112 114 231 390 50
Fragments arranged by size:
1879 1737 596 390 231 114 112 50
SphI G_CATG'C
Cuts at: 0 1473 1746 3547 5109
Size: 1473 273 1801 1562
Fragments arranged by size:
1801 1562 1473 273
SspI AAT'ATT
Cuts at: 0 469 676 4414 5109
Size: 469 207 3738 695
Fragments arranged by size:
3738 695 469 207
StyI C'CwwG_G
Cuts at: 0 338 1196 1268 1340 1562 2531 3293 3710
Size: 338 858 72 72 222 969 762 417
Cuts at: 3710 3734 3815 4154 4298 5042 5109
Size: 24 81 339 144 744 67
Fragments arranged by size:
969 858 762 744 417 339 338 222
144 81 72 72 67 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 909 1188 1281 2506 3946 4552 4570 5109
Size: 909 279 93 1225 1440 606 18 539
Fragments arranged by size:
1440 1225 909 606 539 279 93 18
TatI w'GTAC_w
Cuts at: 0 2453 3026 3053 4536 4790 5109
Size: 2453 573 27 1483 254 319
Fragments arranged by size:
2453 1483 573 319 254 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 167 199 2185 2217 5109
Size: 167 32 1986 32 2892
Fragments arranged by size:
2892 1986 167 32 32
XhoI C'TCGA_G
Cuts at: 0 3616 5109
Size: 3616 1493
XmaI C'CCGG_G
Cuts at: 0 704 1394 3479 5109
Size: 704 690 2085 1630
Fragments arranged by size:
2085 1630 704 690
XmnI GAAnn'nnTTC
Cuts at: 0 2476 2860 3805 4447 5109
Size: 2476 384 945 642 662
Fragments arranged by size:
2476 945 662 642 384
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NcoI NruI
NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SanDI SapI SbfI SfcI
SfiI SmaI SmlI SphI SspI StyI TaqII TatI
TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmtI
BsmBI BspEI BsrFI BstZ17I ClaI CspCI DraI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NsiI
PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XcmI ZraI