Restriction map for
pF1KA1047
(Linear) (Six-base) MAPSORT of: KIAA1047.seq Check: 3620 from: 1 to: 7011
KIAA1047.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4745 5643 7011
Size: 4745 898 1368
Fragments arranged by size:
4745 1368 898
AccI GT'mk_AC
Cuts at: 0 79 4582 7011
Size: 79 4503 2429
Fragments arranged by size:
4503 2429 79
Acc65I G'GTAC_C
Cuts at: 0 3448 7011
Size: 3448 3563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1832 2393 2483 2821 3052 3125 3167 5309
Size: 1832 561 90 338 231 73 42 2142
Cuts at: 5309 6728 7011
Size: 1419 283
Fragments arranged by size:
2142 1832 1419 561 338 283 231 90
73 42
AfeI AGC'GCT
Cuts at: 0 2700 7011
Size: 2700 4311
AflIII A'CryG_T
Cuts at: 0 4391 7011
Size: 4391 2620
AhdI GACnn_n'nnGTC
Cuts at: 0 3746 7011
Size: 3746 3265
AleI CACnn'nnGTG
Cuts at: 0 4396 7011
Size: 4396 2615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2663 2864 7011
Size: 2663 201 4147
Fragments arranged by size:
4147 2663 201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 5046 5078 7011
Size: 578 32 4436 32 1933
Fragments arranged by size:
4436 1933 578 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 121 235 1864 2293 4654 4795 4971 5173
Size: 121 114 1629 429 2361 141 176 202
Cuts at: 5173 5536 6214 6388 6947 7011
Size: 363 678 174 559 64
Fragments arranged by size:
2361 1629 678 559 429 363 202 176
174 141 121 114 64
ApaI G_GGCC'C
Cuts at: 0 3426 5455 7011
Size: 3426 2029 1556
Fragments arranged by size:
3426 2029 1556
ApaLI G'TGCA_C
Cuts at: 0 388 4487 7011
Size: 388 4099 2524
Fragments arranged by size:
4099 2524 388
ApoI r'AATT_y
Cuts at: 0 220 755 1043 3373 3503 7011
Size: 220 535 288 2330 130 3508
Fragments arranged by size:
3508 2330 535 288 220 130
AvaI C'yCGr_G
Cuts at: 0 1706 2276 2659 3482 3731 7011
Size: 1706 570 383 823 249 3280
Fragments arranged by size:
3280 1706 823 570 383 249
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 803 836 7011
Size: 803 33 6175
Fragments arranged by size:
6175 803 33
BamHI G'GATC_C
Cuts at: 0 435 4752 4782 5409 7011
Size: 435 4317 30 627 1602
Fragments arranged by size:
4317 1602 627 435 30
BanI G'GyrC_C
Cuts at: 0 2529 3448 3550 4036 7011
Size: 2529 919 102 486 2975
Fragments arranged by size:
2975 2529 919 486 102
BanII G_rGCy'C
Cuts at: 0 1160 1820 2312 2666 3426 3538 4465 5455
Size: 1160 660 492 354 760 112 927 990
Cuts at: 5455 6128 6137 7011
Size: 673 9 874
Fragments arranged by size:
1160 990 927 874 760 673 660 492
354 112 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1631 2177 3632 6488 6812 7011
Size: 1631 546 1455 2856 324 199
Fragments arranged by size:
2856 1631 1455 546 324 199
BbvCI CC'TCA_GC
Cuts at: 0 409 7011
Size: 409 6602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 209 1498 1532 7011
Size: 175 34 1289 34 5479
Fragments arranged by size:
5479 1289 175 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2880 4642 7011
Size: 2880 1762 2369
Fragments arranged by size:
2880 2369 1762
BclI T'GATC_A
Cuts at: 0 501 933 5834 5847 6668 7011
Size: 501 432 4901 13 821 343
Fragments arranged by size:
4901 821 501 432 343 13
BfrBI ATG'CAT
Cuts at: 0 2545 2949 7011
Size: 2545 404 4062
Fragments arranged by size:
4062 2545 404
BglI GCCn_nnn'nGGC
Cuts at: 0 405 1745 1804 2730 5291 7011
Size: 405 1340 59 926 2561 1720
Fragments arranged by size:
2561 1720 1340 926 405 59
BglII A'GATC_T
Cuts at: 0 1337 6392 7011
Size: 1337 5055 619
Fragments arranged by size:
5055 1337 619
BlpI GC'TnA_GC
Cuts at: 0 2636 5420 6377 6868 7011
Size: 2636 2784 957 491 143
Fragments arranged by size:
2784 2636 957 491 143
Bme1580I G_kGCm'C
Cuts at: 0 392 3426 3553 4204 4491 5455 5819 7011
Size: 392 3034 127 651 287 964 364 1192
Fragments arranged by size:
3034 1192 964 651 392 364 287 127
BmgBI CAC'GTC
Cuts at: 0 328 4394 6114 7011
Size: 328 4066 1720 897
Fragments arranged by size:
4066 1720 897 328
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1947 2324 2661 2899 4981 5357 7011
Size: 286 1661 377 337 238 2082 376 1654
Fragments arranged by size:
2082 1661 1654 377 376 337 286 238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3851 6156 7011
Size: 3851 2305 855
Fragments arranged by size:
3851 2305 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2986 4133 4631 4814 5129 6035 7011
Size: 2986 1147 498 183 315 906 976
Fragments arranged by size:
2986 1147 976 906 498 315 183
Bpu10I CC'TnA_GC
Cuts at: 0 409 650 2123 2346 7011
Size: 409 241 1473 223 4665
Fragments arranged by size:
4665 1473 409 241 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 2326 3257 7011
Size: 230 2096 931 3754
Fragments arranged by size:
3754 2096 931 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 1864 2383 4366 4981 5335 5352 6051 6982
Size: 1864 519 1983 615 354 17 699 931
Cuts at: 6982 7011
Size: 29
Fragments arranged by size:
1983 1864 931 699 615 519 354 29
17
BsaAI yAC'GTr
Cuts at: 0 2693 7011
Size: 2693 4318
BsaBI GATnn'nnATC
Cuts at: 0 725 2441 4694 7011
Size: 725 1716 2253 2317
Fragments arranged by size:
2317 2253 1716 725
BsaHI Gr'CG_yC
Cuts at: 0 6787 7011
Size: 6787 224
BsaWI w'CCGG_w
Cuts at: 0 6156 7011
Size: 6156 855
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 246 276 4961 4991 5046 5076 5097 5127
Size: 246 30 4685 30 55 30 21 30
Cuts at: 5127 5332 5362 7011
Size: 205 30 1649
Fragments arranged by size:
4685 1649 246 205 55 30 30 30
30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 665 2983 5054 6120 6333 6662 6741
Size: 53 612 2318 2071 1066 213 329 79
Cuts at: 6741 6917 7011
Size: 176 94
Fragments arranged by size:
2318 2071 1066 612 329 213 176 94
79 53
BseYI C'CCAG_C
Cuts at: 0 2245 4635 4986 5446 5909 6551 7011
Size: 2245 2390 351 460 463 642 460
Fragments arranged by size:
2390 2245 642 463 460 460 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2204 2309 2715 2859 5522 7011
Size: 2204 105 406 144 2663 1489
Fragments arranged by size:
2663 2204 1489 406 144 105
BsiEI CG_ry'CG
Cuts at: 0 4174 7011
Size: 4174 2837
BsiHKAI G_wGCw'C
Cuts at: 0 392 2214 2456 4168 4491 6903 7011
Size: 392 1822 242 1712 323 2412 108
Fragments arranged by size:
2412 1822 1712 392 323 242 108
BsmI GAATG_Cn'
Cuts at: 0 34 442 3518 3620 3732 4301 7011
Size: 34 408 3076 102 112 569 2710
Fragments arranged by size:
3076 2710 569 408 112 102 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2799 6120 7011
Size: 2799 3321 891
Fragments arranged by size:
3321 2799 891
Bsp1286I G_dGCh'C
Cuts at: 0 392 1160 1820 2214 2312 2456 2666 3426
Size: 392 768 660 394 98 144 210 760
Cuts at: 3426 3538 3553 4168 4204 4465 4491 5455 5819
Size: 112 15 615 36 261 26 964 364
Cuts at: 5819 6128 6137 6903 7011
Size: 309 9 766 108
Fragments arranged by size:
964 768 766 760 660 615 394 392
364 309 261 210 144 112 108 98
36 26 15 9
BspHI T'CATG_A
Cuts at: 0 300 3762 4101 4590 7011
Size: 300 3462 339 489 2421
Fragments arranged by size:
3462 2421 489 339 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2301 4745 5267 5643 7011
Size: 2301 2444 522 376 1368
Fragments arranged by size:
2444 2301 1368 522 376
BsrBI CCG'CTC
Cuts at: 0 2912 5205 5217 5223 6239 7011
Size: 2912 2293 12 6 1016 772
Fragments arranged by size:
2912 2293 1016 772 12 6
BsrDI GCAATG_nn'
Cuts at: 0 1115 2951 4868 7011
Size: 1115 1836 1917 2143
Fragments arranged by size:
2143 1917 1836 1115
BsrGI T'GTAC_A
Cuts at: 0 6756 7011
Size: 6756 255
BssSI C'ACGA_G
Cuts at: 0 2069 7011
Size: 2069 4942
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 1948 4795 5173 5536 6214 7011
Size: 396 1552 2847 378 363 678 797
Fragments arranged by size:
2847 1552 797 678 396 378 363
BstBI TT'CG_AA
Cuts at: 0 195 1047 7011
Size: 195 852 5964
Fragments arranged by size:
5964 852 195
BstEII G'GTnAC_C
Cuts at: 0 4834 7011
Size: 4834 2177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4749 7011
Size: 4749 2262
BstYI r'GATC_y
Cuts at: 0 435 593 1337 2782 2823 3298 4752 4782
Size: 435 158 744 1445 41 475 1454 30
Cuts at: 4782 5409 6392 6944 7011
Size: 627 983 552 67
Fragments arranged by size:
1454 1445 983 744 627 552 475 435
158 67 41 30
BstZ17I GTA'TAC
Cuts at: 0 80 7011
Size: 80 6931
Bsu36I CC'TnA_GG
Cuts at: 0 3404 7011
Size: 3404 3607
BtgI C'CryG_G
Cuts at: 0 2315 3319 4290 5069 6299 7011
Size: 2315 1004 971 779 1230 712
Fragments arranged by size:
2315 1230 1004 971 779 712
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5615 7011
Size: 5615 1396
BtsI GCAGTG_nn'
Cuts at: 0 4432 4709 5437 5492 6967 7011
Size: 4432 277 728 55 1475 44
Fragments arranged by size:
4432 1475 728 277 55 44
DraI TTT'AAA
Cuts at: 0 879 3776 7011
Size: 879 2897 3235
Fragments arranged by size:
3235 2897 879
DrdI GACnn_nn'nnGTC
Cuts at: 0 3921 5294 7011
Size: 3921 1373 1717
Fragments arranged by size:
3921 1717 1373
EaeI y'GGCC_r
Cuts at: 0 4171 4940 5263 5283 7011
Size: 4171 769 323 20 1728
Fragments arranged by size:
4171 1728 769 323 20
EagI C'GGCC_G
Cuts at: 0 4171 7011
Size: 4171 2840
EarI CTCTTCn'nnn_
Cuts at: 0 530 626 1399 1547 1598 1808 2069 2459
Size: 530 96 773 148 51 210 261 390
Cuts at: 2459 6123 6609 6747 6822 7011
Size: 3664 486 138 75 189
Fragments arranged by size:
3664 773 530 486 390 261 210 189
148 138 96 75 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1832 2393 2483 2821 2986 3052 3125 3167
Size: 1832 561 90 338 165 66 73 42
Cuts at: 3167 4133 4631 4814 5129 5309 6035 6728 7011
Size: 966 498 183 315 180 726 693 283
Fragments arranged by size:
1832 966 726 693 561 498 338 315
283 183 180 165 90 73 66 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3641 5045 6641 6713 7011
Size: 3641 1404 1596 72 298
Fragments arranged by size:
3641 1596 1404 298 72
EcoO109I rG'GnC_Cy
Cuts at: 0 1275 3422 4273 4496 5451 5452 5822 6627
Size: 1275 2147 851 223 955 1 370 805
Cuts at: 6627 6812 7011
Size: 185 199
Fragments arranged by size:
2147 1275 955 851 805 370 223 199
185 1
EcoRV GAT'ATC
Cuts at: 0 5573 7011
Size: 5573 1438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 221 1356 2123 2231 3997 7011
Size: 221 1135 767 108 1766 3014
Fragments arranged by size:
3014 1766 1135 767 221 108
HaeII r_GCGC'y
Cuts at: 0 2702 7011
Size: 2702 4309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 540 572 1144 1176 2653 2685 2774 2806
Size: 540 32 572 32 1477 32 89 32
Cuts at: 2806 3081 3113 3200 3232 3732 3764 4210 4242
Size: 275 32 87 32 500 32 446 32
Cuts at: 4242 4665 4697 4868 4900 5562 5594 6223 6255
Size: 423 32 171 32 662 32 629 32
Cuts at: 6255 6384 6416 6799 6831 6979 7011
Size: 129 32 383 32 148 32
Fragments arranged by size:
1477 662 629 572 540 500 446 423
383 275 171 148 129 89 87 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 3966 6280 6510 7011
Size: 3966 2314 230 501
Fragments arranged by size:
3966 2314 501 230
KpnI G_GTAC'C
Cuts at: 0 3452 7011
Size: 3452 3559
MfeI C'AATT_G
Cuts at: 0 1946 2894 7011
Size: 1946 948 4117
Fragments arranged by size:
4117 1946 948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3145 3517 4120 5050 5273 5437 6977 7011
Size: 3145 372 603 930 223 164 1540 34
Fragments arranged by size:
3145 1540 930 603 372 223 164 34
MscI TGG'CCA
Cuts at: 0 4942 7011
Size: 4942 2069
MslI CAynn'nnrTG
Cuts at: 0 1255 3434 4330 4396 5303 5585 7011
Size: 1255 2179 896 66 907 282 1426
Fragments arranged by size:
2179 1426 1255 907 896 282 66
MspA1I CmG'CkG
Cuts at: 0 396 1783 1804 2371 2976 4305 4792 5170
Size: 396 1387 21 567 605 1329 487 378
Cuts at: 5170 5778 6373 7011
Size: 608 595 638
Fragments arranged by size:
1387 1329 638 608 605 595 567 487
396 378 21
NcoI C'CATG_G
Cuts at: 0 5069 6299 7011
Size: 5069 1230 712
Fragments arranged by size:
5069 1230 712
NdeI CA'TA_TG
Cuts at: 0 3711 4059 7011
Size: 3711 348 2952
Fragments arranged by size:
3711 2952 348
NsiI A_TGCA'T
Cuts at: 0 2547 2951 7011
Size: 2547 404 4060
Fragments arranged by size:
4060 2547 404
NspI r_CATG'y
Cuts at: 0 6899 7011
Size: 6899 112
PasI CC'CwG_GG
Cuts at: 0 3881 4197 4465 7011
Size: 3881 316 268 2546
Fragments arranged by size:
3881 2546 316 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 4063 4654 6558 7011
Size: 4063 591 1904 453
Fragments arranged by size:
4063 1904 591 453
PfoI T'CCnGG_A
Cuts at: 0 2963 5145 7011
Size: 2963 2182 1866
Fragments arranged by size:
2963 2182 1866
PmlI CAC'GTG
Cuts at: 0 2693 7011
Size: 2693 4318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5046 5078 7011
Size: 5046 32 1933
Fragments arranged by size:
5046 1933 32
PpuMI rG'GwC_Cy
Cuts at: 0 1275 4496 5822 6627 7011
Size: 1275 3221 1326 805 384
Fragments arranged by size:
3221 1326 1275 805 384
PshAI GACnn'nnGTC
Cuts at: 0 3632 7011
Size: 3632 3379
PsiI TTA'TAA
Cuts at: 0 1460 3202 6845 7011
Size: 1460 1742 3643 166
Fragments arranged by size:
3643 1742 1460 166
PspOMI G'GGCC_C
Cuts at: 0 3422 5451 7011
Size: 3422 2029 1560
Fragments arranged by size:
3422 2029 1560
PstI C_TGCA'G
Cuts at: 0 423 1782 1797 4716 4791 5241 5532 5660
Size: 423 1359 15 2919 75 450 291 128
Cuts at: 5660 6219 7011
Size: 559 792
Fragments arranged by size:
2919 1359 792 559 450 423 291 128
75 15
PvuII CAG'CTG
Cuts at: 0 1783 2371 5170 5778 6373 7011
Size: 1783 588 2799 608 595 638
Fragments arranged by size:
2799 1783 638 608 595 588
SanDI GG'GwC_CC
Cuts at: 0 6627 7011
Size: 6627 384
SapI GCTCTTCn'nnn_
Cuts at: 0 1808 2069 7011
Size: 1808 261 4942
Fragments arranged by size:
4942 1808 261
ScaI AGT'ACT
Cuts at: 0 6876 7011
Size: 6876 135
SexAI A'CCwGG_T
Cuts at: 0 845 1756 6033 7011
Size: 845 911 4277 978
Fragments arranged by size:
4277 978 911 845
SfcI C'TryA_G
Cuts at: 0 419 1778 1793 1856 4712 4787 5237 5528
Size: 419 1359 15 63 2856 75 450 291
Cuts at: 5528 5656 6215 7011
Size: 128 559 796
Fragments arranged by size:
2856 1359 796 559 450 419 291 128
75 63 15
SmaI CCC'GGG
Cuts at: 0 1708 7011
Size: 1708 5303
SmlI C'TyrA_G
Cuts at: 0 245 2276 2305 3272 7011
Size: 245 2031 29 967 3739
Fragments arranged by size:
3739 2031 967 245 29
SpeI A'CTAG_T
Cuts at: 0 259 7011
Size: 259 6752
SspI AAT'ATT
Cuts at: 0 3720 7011
Size: 3720 3291
StuI AGG'CCT
Cuts at: 0 243 5471 6087 7011
Size: 243 5228 616 924
Fragments arranged by size:
5228 924 616 243
StyI C'CwwG_G
Cuts at: 0 27 815 1643 4111 5069 5373 5466 6299
Size: 27 788 828 2468 958 304 93 833
Cuts at: 6299 7011
Size: 712
Fragments arranged by size:
2468 958 833 828 788 712 304 93
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 242 2343 3297 4551 5168 6312 7011
Size: 242 2101 954 1254 617 1144 699
Fragments arranged by size:
2101 1254 1144 954 699 617 242
TatI w'GTAC_w
Cuts at: 0 2332 2359 6756 6874 7011
Size: 2332 27 4397 118 137
Fragments arranged by size:
4397 2332 137 118 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2891 2923 4961 4993 5073 5105 7011
Size: 2891 32 2038 32 80 32 1906
Fragments arranged by size:
2891 2038 1906 80 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 326 4666 6993 7011
Size: 326 4340 2327 18
Fragments arranged by size:
4340 2327 326 18
XbaI T'CTAG_A
Cuts at: 0 6436 7011
Size: 6436 575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2601 3053 3174 3291 4083 5380 6797 7011
Size: 2601 452 121 117 792 1297 1417 214
Fragments arranged by size:
2601 1417 1297 792 452 214 121 117
XhoI C'TCGA_G
Cuts at: 0 2276 7011
Size: 2276 4735
XmaI C'CCGG_G
Cuts at: 0 1706 7011
Size: 1706 5305
XmnI GAAnn'nnTTC
Cuts at: 0 272 1073 3065 3110 7011
Size: 272 801 1992 45 3901
Fragments arranged by size:
3901 1992 801 272 45
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuII SanDI SapI ScaI SexAI SfcI
SmaI SmlI SpeI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AscI AseI AsiSI AvrII
BbeI BmtI BsiWI BspEI BsrFI BssHII ClaI CspCI
DraIII EciI EcoICRI EcoRI FseI FspI FspAI HincII
HpaI KasI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PciI PmeI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SwaI ZraI