Restriction map for
pF1KSDA1048
(Linear) (Six-base) MAPSORT of: KIAA1048.seq Check: 2876 from: 1 to: 2589
KIAA1048.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 2077 2589
Size: 2077 512
AclI AA'CG_TT
Cuts at: 0 2054 2589
Size: 2054 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 2051 2144 2474 2589
Size: 550 1501 93 330 115
Fragments arranged by size:
1501 550 330 115 93
AfeI AGC'GCT
Cuts at: 0 1160 2589
Size: 1160 1429
AhdI GACnn_n'nnGTC
Cuts at: 0 133 2589
Size: 133 2456
AlwNI CAG_nnn'CTG
Cuts at: 0 423 892 1281 1356 1759 2589
Size: 423 469 389 75 403 830
Fragments arranged by size:
830 469 423 403 389 75
ApoI r'AATT_y
Cuts at: 0 601 1587 2589
Size: 601 986 1002
Fragments arranged by size:
1002 986 601
AvaI C'yCGr_G
Cuts at: 0 81 2173 2589
Size: 81 2092 416
Fragments arranged by size:
2092 416 81
BamHI G'GATC_C
Cuts at: 0 1210 2589
Size: 1210 1379
BanII G_rGCy'C
Cuts at: 0 52 741 2589
Size: 52 689 1848
Fragments arranged by size:
1848 689 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 2589
Size: 105 2484
BbvCI CC'TCA_GC
Cuts at: 0 1982 2589
Size: 1982 607
BfrBI ATG'CAT
Cuts at: 0 2499 2589
Size: 2499 90
BglI GCCn_nnn'nGGC
Cuts at: 0 1021 2589
Size: 1021 1568
BlpI GC'TnA_GC
Cuts at: 0 2190 2589
Size: 2190 399
Bme1580I G_kGCm'C
Cuts at: 0 299 958 1895 2589
Size: 299 659 937 694
Fragments arranged by size:
937 694 659 299
BmrI ACTGGGnnnn_n'
Cuts at: 0 1780 2589
Size: 1780 809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 235 439 1951 2589
Size: 42 193 204 1512 638
Fragments arranged by size:
1512 638 204 193 42
Bpu10I CC'TnA_GC
Cuts at: 0 1364 1982 2589
Size: 1364 618 607
Fragments arranged by size:
1364 618 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 928 1390 2589
Size: 833 95 462 1199
Fragments arranged by size:
1199 833 462 95
BsaAI yAC'GTr
Cuts at: 0 2081 2589
Size: 2081 508
BsaHI Gr'CG_yC
Cuts at: 0 1333 2589
Size: 1333 1256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1806 1836 2004 2034 2589
Size: 1806 30 168 30 555
Fragments arranged by size:
1806 555 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 1304 2024 2589
Size: 80 1224 720 565
Fragments arranged by size:
1224 720 565 80
BseYI C'CCAG_C
Cuts at: 0 1040 1114 1270 1382 1520 1724 1888 1958
Size: 1040 74 156 112 138 204 164 70
Cuts at: 1958 2589
Size: 631
Fragments arranged by size:
1040 631 204 164 156 138 112 74
70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1946 2589
Size: 1946 643
BsiHKAI G_wGCw'C
Cuts at: 0 368 455 681 2589
Size: 368 87 226 1908
Fragments arranged by size:
1908 368 226 87
Bsp1286I G_dGCh'C
Cuts at: 0 52 299 368 455 681 741 958 1895
Size: 52 247 69 87 226 60 217 937
Cuts at: 1895 2589
Size: 694
Fragments arranged by size:
937 694 247 226 217 87 69 60
52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 2589
Size: 123 2466
BsrBI CCG'CTC
Cuts at: 0 1363 2589
Size: 1363 1226
BsrDI GCAATG_nn'
Cuts at: 0 204 2589
Size: 204 2385
BsrFI r'CCGG_y
Cuts at: 0 1452 2589
Size: 1452 1137
BsrGI T'GTAC_A
Cuts at: 0 698 969 2589
Size: 698 271 1620
Fragments arranged by size:
1620 698 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1581 2589
Size: 1581 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1888 2589
Size: 1888 701
BstYI r'GATC_y
Cuts at: 0 285 1062 1210 1849 2433 2589
Size: 285 777 148 639 584 156
Fragments arranged by size:
777 639 584 285 156 148
Bsu36I CC'TnA_GG
Cuts at: 0 1199 2589
Size: 1199 1390
BtsI GCAGTG_nn'
Cuts at: 0 99 858 2589
Size: 99 759 1731
Fragments arranged by size:
1731 759 99
EaeI y'GGCC_r
Cuts at: 0 393 1640 2589
Size: 393 1247 949
Fragments arranged by size:
1247 949 393
EarI CTCTTCn'nnn_
Cuts at: 0 102 1168 1405 2348 2408 2589
Size: 102 1066 237 943 60 181
Fragments arranged by size:
1066 943 237 181 102 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 2589
Size: 172 2417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 235 439 550 1951 2051 2144 2474
Size: 42 193 204 111 1401 100 93 330
Cuts at: 2474 2589
Size: 115
Fragments arranged by size:
1401 330 204 193 115 111 100 93
42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2069 2589
Size: 2069 520
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1938 2589
Size: 525 1413 651
Fragments arranged by size:
1413 651 525
HaeII r_GCGC'y
Cuts at: 0 423 593 1162 2589
Size: 423 170 569 1427
Fragments arranged by size:
1427 569 423 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 2092 2124 2589
Size: 119 32 1941 32 465
Fragments arranged by size:
1941 465 119 32 32
HincII GTy'rAC
Cuts at: 0 234 693 2541 2589
Size: 234 459 1848 48
Fragments arranged by size:
1848 459 234 48
HindIII A'AGCT_T
Cuts at: 0 994 2152 2179 2589
Size: 994 1158 27 410
Fragments arranged by size:
1158 994 410 27
HpaI GTT'AAC
Cuts at: 0 2541 2589
Size: 2541 48
MfeI C'AATT_G
Cuts at: 0 1088 1532 2589
Size: 1088 444 1057
Fragments arranged by size:
1088 1057 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 1827 2589
Size: 860 967 762
Fragments arranged by size:
967 860 762
MscI TGG'CCA
Cuts at: 0 395 1642 2589
Size: 395 1247 947
Fragments arranged by size:
1247 947 395
MslI CAynn'nnrTG
Cuts at: 0 373 2589
Size: 373 2216
MspA1I CmG'CkG
Cuts at: 0 62 1027 1638 1665 1738 1962 1975 1981
Size: 62 965 611 27 73 224 13 6
Cuts at: 1981 2122 2194 2589
Size: 141 72 395
Fragments arranged by size:
965 611 395 224 141 73 72 62
27 13 6
NaeI GCC'GGC
Cuts at: 0 1454 2589
Size: 1454 1135
NgoMIV G'CCGG_C
Cuts at: 0 1452 2589
Size: 1452 1137
NsiI A_TGCA'T
Cuts at: 0 2501 2589
Size: 2501 88
NspI r_CATG'y
Cuts at: 0 228 858 2589
Size: 228 630 1731
Fragments arranged by size:
1731 630 228
PasI CC'CwG_GG
Cuts at: 0 1350 2550 2589
Size: 1350 1200 39
Fragments arranged by size:
1350 1200 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 2550 2589
Size: 2550 39
PpuMI rG'GwC_Cy
Cuts at: 0 525 1938 2589
Size: 525 1413 651
Fragments arranged by size:
1413 651 525
PsiI TTA'TAA
Cuts at: 0 2060 2589
Size: 2060 529
PstI C_TGCA'G
Cuts at: 0 1209 1743 1980 2589
Size: 1209 534 237 609
Fragments arranged by size:
1209 609 534 237
PvuII CAG'CTG
Cuts at: 0 1027 1638 1665 1738 1962 1975 1981 2122
Size: 1027 611 27 73 224 13 6 141
Cuts at: 2122 2194 2589
Size: 72 395
Fragments arranged by size:
1027 611 395 224 141 73 72 27
13 6
SanDI GG'GwC_CC
Cuts at: 0 1938 2589
Size: 1938 651
SexAI A'CCwGG_T
Cuts at: 0 914 2589
Size: 914 1675
SfcI C'TryA_G
Cuts at: 0 1205 1739 1976 2589
Size: 1205 534 237 613
Fragments arranged by size:
1205 613 534 237
SmlI C'TyrA_G
Cuts at: 0 848 943 1405 2589
Size: 848 95 462 1184
Fragments arranged by size:
1184 848 462 95
SspI AAT'ATT
Cuts at: 0 306 2589
Size: 306 2283
StuI AGG'CCT
Cuts at: 0 1107 2589
Size: 1107 1482
StyI C'CwwG_G
Cuts at: 0 1557 2226 2579 2589
Size: 1557 669 353 10
Fragments arranged by size:
1557 669 353 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 1284 2589
Size: 572 712 1305
Fragments arranged by size:
1305 712 572
TatI w'GTAC_w
Cuts at: 0 698 969 1506 2589
Size: 698 271 537 1083
Fragments arranged by size:
1083 698 537 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 195 227 2589
Size: 195 32 2362
Fragments arranged by size:
2362 195 32
XmnI GAAnn'nnTTC
Cuts at: 0 10 2589
Size: 10 2579
Enzymes that do cut:
AccI AclI AcuI AfeI AhdI AlwNI ApoI AvaI
BamHI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NaeI NgoMIV
NsiI NspI PasI PflMI PpuMI PsiI PstI PvuII
SanDI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BciVI BclI BglII BmgBI BmtI
BplI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI KasI KpnI
MluI NarI NcoI NdeI NheI NotI NruI PacI
PciI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI