Restriction map for
pF1KSDA1051
(Linear) (Six-base) MAPSORT of: KIAA1051.seq Check: 3564 from: 1 to: 1071
KIAA1051.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 512 1071
Size: 512 559
Acc65I G'GTAC_C
Cuts at: 0 908 980 996 1071
Size: 908 72 16 75
Fragments arranged by size:
908 75 72 16
AflIII A'CryG_T
Cuts at: 0 582 1071
Size: 582 489
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 1071
Size: 674 32 365
Fragments arranged by size:
674 365 32
ApaI G_GGCC'C
Cuts at: 0 178 1071
Size: 178 893
ApoI r'AATT_y
Cuts at: 0 107 792 829 1071
Size: 107 685 37 242
Fragments arranged by size:
685 242 107 37
AvrII C'CTAG_G
Cuts at: 0 276 1071
Size: 276 795
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 964 988 997 1021 1071
Size: 964 24 9 24 50
Fragments arranged by size:
964 50 24 24 9
BanI G'GyrC_C
Cuts at: 0 158 908 980 996 1071
Size: 158 750 72 16 75
Fragments arranged by size:
750 158 75 72 16
BanII G_rGCy'C
Cuts at: 0 178 687 744 1071
Size: 178 509 57 327
Fragments arranged by size:
509 327 178 57
BbeI G_GCGC'C
Cuts at: 0 162 1071
Size: 162 909
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 1071
Size: 806 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 76 77 110 1071
Size: 43 33 1 33 961
Fragments arranged by size:
961 43 33 33 1
BciVI GTATCCnnnnn_n'
Cuts at: 0 436 782 844 1071
Size: 436 346 62 227
Fragments arranged by size:
436 346 227 62
BclI T'GATC_A
Cuts at: 0 81 959 1071
Size: 81 878 112
Fragments arranged by size:
878 112 81
BlpI GC'TnA_GC
Cuts at: 0 292 1071
Size: 292 779
Bme1580I G_kGCm'C
Cuts at: 0 178 1071
Size: 178 893
BmgBI CAC'GTC
Cuts at: 0 510 1071
Size: 510 561
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 569 886 907 1071
Size: 172 397 317 21 164
Fragments arranged by size:
397 317 172 164 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 578 679 1071
Size: 479 99 101 392
Fragments arranged by size:
479 392 101 99
BsaHI Gr'CG_yC
Cuts at: 0 159 1071
Size: 159 912
BsaWI w'CCGG_w
Cuts at: 0 993 1071
Size: 993 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 240 270 380 410 674 704 1071
Size: 240 30 110 30 264 30 367
Fragments arranged by size:
367 264 240 110 30 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 298 325 509 564 744 1071
Size: 298 27 184 55 180 327
Fragments arranged by size:
327 298 184 180 55 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 917 1071
Size: 917 154
Bsp1286I G_dGCh'C
Cuts at: 0 178 298 325 509 564 687 744 1071
Size: 178 120 27 184 55 123 57 327
Fragments arranged by size:
327 184 178 123 120 57 55 27
BsrGI T'GTAC_A
Cuts at: 0 488 1071
Size: 488 583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 377 1071
Size: 377 694
BtsI GCAGTG_nn'
Cuts at: 0 363 1071
Size: 363 708
ClaI AT'CG_AT
Cuts at: 0 53 1071
Size: 53 1018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 122 157 1071
Size: 122 35 914
Fragments arranged by size:
914 122 35
EaeI y'GGCC_r
Cuts at: 0 130 1071
Size: 130 941
EarI CTCTTCn'nnn_
Cuts at: 0 623 697 1071
Size: 623 74 374
Fragments arranged by size:
623 374 74
EcoICRI GAG'CTC
Cuts at: 0 742 1071
Size: 742 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 578 679 1071
Size: 479 99 101 392
Fragments arranged by size:
479 392 101 99
EcoRI G'AATT_C
Cuts at: 0 107 829 1071
Size: 107 722 242
Fragments arranged by size:
722 242 107
HaeII r_GCGC'y
Cuts at: 0 162 1071
Size: 162 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 220 240 252 272 320 352
Size: 35 32 153 20 12 20 48 32
Cuts at: 352 363 395 1071
Size: 11 32 676
Fragments arranged by size:
676 153 48 35 32 32 32 20
20 12 11
HincII GTy'rAC
Cuts at: 0 210 534 1071
Size: 210 324 537
Fragments arranged by size:
537 324 210
KasI G'GCGC_C
Cuts at: 0 158 1071
Size: 158 913
KpnI G_GTAC'C
Cuts at: 0 912 984 1000 1071
Size: 912 72 16 71
Fragments arranged by size:
912 72 71 16
MfeI C'AATT_G
Cuts at: 0 149 1071
Size: 149 922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1071
Size: 688 383
MscI TGG'CCA
Cuts at: 0 132 1071
Size: 132 939
MslI CAynn'nnrTG
Cuts at: 0 89 547 1071
Size: 89 458 524
Fragments arranged by size:
524 458 89
NarI GG'CG_CC
Cuts at: 0 159 1071
Size: 159 912
NdeI CA'TA_TG
Cuts at: 0 870 1071
Size: 870 201
NspI r_CATG'y
Cuts at: 0 586 1071
Size: 586 485
PciI A'CATG_T
Cuts at: 0 582 1071
Size: 582 489
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 1071
Size: 439 632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 674 706 1071
Size: 674 32 365
Fragments arranged by size:
674 365 32
PspOMI G'GGCC_C
Cuts at: 0 174 1071
Size: 174 897
SacI G_AGCT'C
Cuts at: 0 744 1071
Size: 744 327
SapI GCTCTTCn'nnn_
Cuts at: 0 623 1071
Size: 623 448
SfoI GGC'GCC
Cuts at: 0 160 1071
Size: 160 911
StyI C'CwwG_G
Cuts at: 0 276 1071
Size: 276 795
TatI w'GTAC_w
Cuts at: 0 488 1071
Size: 488 583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 288 320 380 412 954 986 1071
Size: 288 32 60 32 542 32 85
Fragments arranged by size:
542 288 85 60 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 252 1071
Size: 252 819
Enzymes that do cut:
AccI Acc65I AflIII AloI ApaI ApoI AvrII BaeI
BanI BanII BbeI BbsI BcgI BciVI BclI BlpI
Bme1580I BmgBI BmrI BpmI BsaHI BsaWI BsaXI BsiHKAI
BsmBI Bsp1286I BsrGI BtgZI BtsI ClaI CspCI EaeI
EarI EcoICRI Eco57MI EcoRI HaeII Hin4I HincII KasI
KpnI MfeI MmeI MscI MslI NarI NdeI NspI
PciI PflMI PpiI PspOMI SacI SapI SfoI StyI
TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaLI AscI AseI AsiSI AvaI
BamHI BbvCI BfrBI BglI BglII BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BseRI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI DraI DraIII DrdI EagI EciI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HindIII HpaI
MluI MspA1I NaeI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII XbaI XcmI XhoI XmaI XmnI
ZraI