Restriction map for
pF1KA1062
(Linear) (Six-base) MAPSORT of: KIAA1062.seq Check: 6108 from: 1 to: 7305
KIAA1062.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 581 721 1541 2963 4630 4930 5477 6449
Size: 581 140 820 1422 1667 300 547 972
Cuts at: 6449 6996 7305
Size: 547 309
Fragments arranged by size:
1667 1422 972 820 581 547 547 309
300 140
AatII G_ACGT'C
Cuts at: 0 187 4175 4876 7305
Size: 187 3988 701 2429
Fragments arranged by size:
3988 2429 701 187
AccI GT'mk_AC
Cuts at: 0 566 1403 2147 2372 2993 6067 6173 7305
Size: 566 837 744 225 621 3074 106 1132
Fragments arranged by size:
3074 1132 837 744 621 566 225 106
Acc65I G'GTAC_C
Cuts at: 0 3644 6605 7305
Size: 3644 2961 700
Fragments arranged by size:
3644 2961 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2332 2622 3517 4321 5128 5821 6376 6835
Size: 2332 290 895 804 807 693 555 459
Cuts at: 6835 7305
Size: 470
Fragments arranged by size:
2332 895 807 804 693 555 470 459
290
AflIII A'CryG_T
Cuts at: 0 2483 5519 5597 5711 7305
Size: 2483 3036 78 114 1594
Fragments arranged by size:
3036 2483 1594 114 78
AgeI A'CCGG_T
Cuts at: 0 5075 6821 7305
Size: 5075 1746 484
Fragments arranged by size:
5075 1746 484
AleI CACnn'nnGTG
Cuts at: 0 2020 4624 6943 7305
Size: 2020 2604 2319 362
Fragments arranged by size:
2604 2319 2020 362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 833 2071 7305
Size: 833 1238 5234
Fragments arranged by size:
5234 1238 833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3934 3966 7051 7083 7305
Size: 3934 32 3085 32 222
Fragments arranged by size:
3934 3085 222 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 27 378 894 1668 3981 4746 5486 6813
Size: 27 351 516 774 2313 765 740 1327
Cuts at: 6813 7305
Size: 492
Fragments arranged by size:
2313 1327 774 765 740 516 492 351
27
ApaI G_GGCC'C
Cuts at: 0 400 1132 1940 3688 3729 4045 4661 5142
Size: 400 732 808 1748 41 316 616 481
Cuts at: 5142 7275 7305
Size: 2133 30
Fragments arranged by size:
2133 1748 808 732 616 481 400 316
41 30
ApaLI G'TGCA_C
Cuts at: 0 1348 1861 2239 2746 6753 7305
Size: 1348 513 378 507 4007 552
Fragments arranged by size:
4007 1348 552 513 507 378
AvaI C'yCGr_G
Cuts at: 0 358 399 408 559 726 1273 1576 6431
Size: 358 41 9 151 167 547 303 4855
Cuts at: 6431 6488 7219 7305
Size: 57 731 86
Fragments arranged by size:
4855 731 547 358 303 167 151 86
57 41 9
AvrII C'CTAG_G
Cuts at: 0 645 7305
Size: 645 6660
BamHI G'GATC_C
Cuts at: 0 743 1700 1982 7305
Size: 743 957 282 5323
Fragments arranged by size:
5323 957 743 282
BanI G'GyrC_C
Cuts at: 0 174 403 936 1045 3628 3644 3878 4615
Size: 174 229 533 109 2583 16 234 737
Cuts at: 4615 4878 4936 4970 5131 6550 6605 6791 6932
Size: 263 58 34 161 1419 55 186 141
Cuts at: 6932 7305
Size: 373
Fragments arranged by size:
2583 1419 737 533 373 263 234 229
186 174 161 141 109 58 55 34
16
BanII G_rGCy'C
Cuts at: 0 64 352 359 400 482 635 727 777
Size: 64 288 7 41 82 153 92 50
Cuts at: 777 857 1132 1303 1396 1940 2584 2948 3335
Size: 80 275 171 93 544 644 364 387
Cuts at: 3335 3446 3688 3729 3752 3821 4045 4138 4661
Size: 111 242 41 23 69 224 93 523
Cuts at: 4661 4910 5142 5870 6254 6425 6507 6635 7196
Size: 249 232 728 384 171 82 128 561
Cuts at: 7196 7220 7275 7305
Size: 24 55 30
Fragments arranged by size:
728 644 561 544 523 387 384 364
288 275 249 242 232 224 171 171
153 128 111 93 93 92 82 82
80 69 64 55 50 41 41 30
24 23 7
BbeI G_GCGC'C
Cuts at: 0 178 940 7305
Size: 178 762 6365
Fragments arranged by size:
6365 762 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 2221 5424 7305
Size: 431 1790 3203 1881
Fragments arranged by size:
3203 1881 1790 431
BbvCI CC'TCA_GC
Cuts at: 0 1145 3910 7305
Size: 1145 2765 3395
Fragments arranged by size:
3395 2765 1145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1417 1451 5355 5389 7305
Size: 1417 34 3904 34 1916
Fragments arranged by size:
3904 1916 1417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1665 6208 7305
Size: 1665 4543 1097
Fragments arranged by size:
4543 1665 1097
BclI T'GATC_A
Cuts at: 0 6842 7305
Size: 6842 463
BfrBI ATG'CAT
Cuts at: 0 3901 7305
Size: 3901 3404
BglI GCCn_nnn'nGGC
Cuts at: 0 227 650 2434 5758 7305
Size: 227 423 1784 3324 1547
Fragments arranged by size:
3324 1784 1547 423 227
BglII A'GATC_T
Cuts at: 0 83 4379 7305
Size: 83 4296 2926
Fragments arranged by size:
4296 2926 83
BlpI GC'TnA_GC
Cuts at: 0 851 1206 3028 3733 7159 7305
Size: 851 355 1822 705 3426 146
Fragments arranged by size:
3426 1822 851 705 355 146
Bme1580I G_kGCm'C
Cuts at: 0 223 400 406 1132 1352 1865 1940 2030
Size: 223 177 6 726 220 513 75 90
Cuts at: 2030 2243 2480 2750 2856 3158 3208 3631 3688
Size: 213 237 270 106 302 50 423 57
Cuts at: 3688 3729 4045 4661 4939 4973 5142 5368 6757
Size: 41 316 616 278 34 169 226 1389
Cuts at: 6757 7203 7275 7305
Size: 446 72 30
Fragments arranged by size:
1389 726 616 513 446 423 316 302
278 270 237 226 223 220 213 177
169 106 90 75 72 57 50 41
34 30 6
BmgBI CAC'GTC
Cuts at: 0 873 2034 5600 6096 6882 7305
Size: 873 1161 3566 496 786 423
Fragments arranged by size:
3566 1161 873 786 496 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 1118 1637 2253 3756 4092 4470 7305
Size: 1118 519 616 1503 336 378 2835
Fragments arranged by size:
2835 1503 1118 616 519 378 336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3299 6990 7053 7305
Size: 3299 3691 63 252
Fragments arranged by size:
3691 3299 252 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 657 721 1528 1869 3349 3736 3904
Size: 400 257 64 807 341 1480 387 168
Cuts at: 3904 3961 4003 4747 6352 6529 6538 7123 7305
Size: 57 42 744 1605 177 9 585 182
Fragments arranged by size:
1605 1480 807 744 585 400 387 341
257 182 177 168 64 57 42 9
Bpu10I CC'TnA_GC
Cuts at: 0 1145 2467 3284 3910 4672 7305
Size: 1145 1322 817 626 762 2633
Fragments arranged by size:
2633 1322 1145 817 762 626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1402 2185 3256 3580 3607 3940 4615
Size: 307 1095 783 1071 324 27 333 675
Cuts at: 4615 6907 6943 7305
Size: 2292 36 362
Fragments arranged by size:
2292 1095 1071 783 675 362 333 324
307 36 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 888 2917 3063 3804 7305
Size: 888 2029 146 741 3501
Fragments arranged by size:
3501 2029 888 741 146
BsaAI yAC'GTr
Cuts at: 0 1860 2055 2343 2475 2490 2729 5520 5712
Size: 1860 195 288 132 15 239 2791 192
Cuts at: 5712 7305
Size: 1593
Fragments arranged by size:
2791 1860 1593 288 239 195 192 132
15
BsaBI GATnn'nnATC
Cuts at: 0 88 7305
Size: 88 7217
BsaHI Gr'CG_yC
Cuts at: 0 175 184 937 2705 4048 4123 4172 4565
Size: 175 9 753 1768 1343 75 49 393
Cuts at: 4565 4873 5996 6131 6260 7305
Size: 308 1123 135 129 1045
Fragments arranged by size:
1768 1343 1123 1045 753 393 308 175
135 129 75 49 9
BsaWI w'CCGG_w
Cuts at: 0 665 857 5075 6821 7305
Size: 665 192 4218 1746 484
Fragments arranged by size:
4218 1746 665 484 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 1285 1315 3934 3961 3964 3991
Size: 250 30 1005 30 2619 27 3 27
Cuts at: 3991 5662 5692 6994 7024 7305
Size: 1671 30 1302 30 281
Fragments arranged by size:
2619 1671 1302 1005 281 250 30 30
30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 915 1162 1333 1768 2518 2896 2935
Size: 688 227 247 171 435 750 378 39
Cuts at: 2935 2953 2956 3304 3982 4543 6751 7276 7279
Size: 18 3 348 678 561 2208 525 3
Cuts at: 7279 7305
Size: 26
Fragments arranged by size:
2208 750 688 678 561 525 435 378
348 247 227 171 39 26 18 3
3
BseYI C'CCAG_C
Cuts at: 0 117 330 340 735 887 894 1074 2808
Size: 117 213 10 395 152 7 180 1734
Cuts at: 2808 3835 4493 4574 4653 4855 4954 5118 5698
Size: 1027 658 81 79 202 99 164 580
Cuts at: 5698 6609 7020 7274 7305
Size: 911 411 254 31
Fragments arranged by size:
1734 1027 911 658 580 411 395 254
213 202 180 164 152 117 99 81
79 31 10 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1035 1555 2077 2293 3379 4902 5056
Size: 699 336 520 522 216 1086 1523 154
Cuts at: 5056 6394 6967 7305
Size: 1338 573 338
Fragments arranged by size:
1523 1338 1086 699 573 522 520 338
336 216 154
BsiEI CG_ry'CG
Cuts at: 0 1407 1953 5061 6783 7305
Size: 1407 546 3108 1722 522
Fragments arranged by size:
3108 1722 1407 546 522
BsiHKAI G_wGCw'C
Cuts at: 0 359 482 857 1207 1303 1352 1865 2114
Size: 359 123 375 350 96 49 513 249
Cuts at: 2114 2195 2243 2750 3224 3335 3752 3821 4028
Size: 81 48 507 474 111 417 69 207
Cuts at: 4028 4087 4591 4631 5789 6122 6316 6425 6440
Size: 59 504 40 1158 333 194 109 15
Cuts at: 6440 6757 6971 7196 7305
Size: 317 214 225 109
Fragments arranged by size:
1158 513 507 504 474 417 375 359
350 333 317 249 225 214 207 194
123 111 109 109 96 81 69 59
49 48 40 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 4842 6122 7108 7305
Size: 230 4612 1280 986 197
Fragments arranged by size:
4612 1280 986 230 197
Bsp1286I G_dGCh'C
Cuts at: 0 64 223 352 359 400 406 482 635
Size: 64 159 129 7 41 6 76 153
Cuts at: 635 727 777 857 1132 1207 1303 1352 1396
Size: 92 50 80 275 75 96 49 44
Cuts at: 1396 1865 1940 2030 2114 2195 2243 2480 2584
Size: 469 75 90 84 81 48 237 104
Cuts at: 2584 2750 2856 2948 3158 3208 3224 3335 3446
Size: 166 106 92 210 50 16 111 111
Cuts at: 3446 3631 3688 3729 3752 3821 4028 4045 4087
Size: 185 57 41 23 69 207 17 42
Cuts at: 4087 4138 4591 4631 4661 4910 4939 4973 5142
Size: 51 453 40 30 249 29 34 169
Cuts at: 5142 5368 5789 5870 6122 6254 6316 6425 6440
Size: 226 421 81 252 132 62 109 15
Cuts at: 6440 6507 6635 6757 6971 7196 7203 7220 7275
Size: 67 128 122 214 225 7 17 55
Cuts at: 7275 7305
Size: 30
Fragments arranged by size:
469 453 421 275 252 249 237 226
225 214 210 207 185 169 166 159
153 132 129 128 122 111 111 109
106 104 96 92 92 90 84 81
81 80 76 75 75 69 67 64
62 57 55 51 50 50 49 48
44 42 41 41 40 34 30 30
29 23 17 17 16 15 7 7
6
BspEI T'CCGG_A
Cuts at: 0 665 857 7305
Size: 665 192 6448
Fragments arranged by size:
6448 665 192
BspHI T'CATG_A
Cuts at: 0 1355 7305
Size: 1355 5950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 721 983 1541 2963 3563 4630 4829
Size: 581 140 262 558 1422 600 1067 199
Cuts at: 4829 4930 5051 5270 5363 5477 5563 5594 6449
Size: 101 121 219 93 114 86 31 855
Cuts at: 6449 6996 7305
Size: 547 309
Fragments arranged by size:
1422 1067 855 600 581 558 547 309
262 219 199 140 121 114 101 93
86 31
BsrBI CCG'CTC
Cuts at: 0 3615 6108 6930 7269 7305
Size: 3615 2493 822 339 36
Fragments arranged by size:
3615 2493 822 339 36
BsrDI GCAATG_nn'
Cuts at: 0 3569 3901 7305
Size: 3569 332 3404
Fragments arranged by size:
3569 3404 332
BsrFI r'CCGG_y
Cuts at: 0 190 1228 1611 2195 2263 2611 2909 3112
Size: 190 1038 383 584 68 348 298 203
Cuts at: 3112 3230 4544 4939 5020 5075 5415 5589 6301
Size: 118 1314 395 81 55 340 174 712
Cuts at: 6301 6543 6821 7305
Size: 242 278 484
Fragments arranged by size:
1314 1038 712 584 484 395 383 348
340 298 278 242 203 190 174 118
81 68 55
BsrGI T'GTAC_A
Cuts at: 0 6449 7305
Size: 6449 856
BssHII G'CGCG_C
Cuts at: 0 2001 3469 7305
Size: 2001 1468 3836
Fragments arranged by size:
3836 2001 1468
BssSI C'ACGA_G
Cuts at: 0 1314 1345 4582 5437 7305
Size: 1314 31 3237 855 1868
Fragments arranged by size:
3237 1868 1314 855 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 27 1162 1533 2118 3219 6117 7305
Size: 18 9 1135 371 585 1101 2898 1188
Fragments arranged by size:
2898 1188 1135 1101 585 371 18 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1650 2697 3435 4419 4501 4868 4962 6078
Size: 1650 1047 738 984 82 367 94 1116
Cuts at: 6078 6645 7305
Size: 567 660
Fragments arranged by size:
1650 1116 1047 984 738 660 567 367
94 82
BstYI r'GATC_y
Cuts at: 0 83 427 743 1493 1700 1871 1910 1982
Size: 83 344 316 750 207 171 39 72
Cuts at: 1982 3182 3293 4379 6467 7305
Size: 1200 111 1086 2088 838
Fragments arranged by size:
2088 1200 1086 838 750 344 316 207
171 111 83 72 39
BtgI C'CryG_G
Cuts at: 0 64 269 946 2153 2219 2708 2848 2900
Size: 64 205 677 1207 66 489 140 52
Cuts at: 2900 2928 3395 3446 3585 4143 4317 4418 4500
Size: 28 467 51 139 558 174 101 82
Cuts at: 4500 5147 5756 5862 5967 5981 6584 7187 7305
Size: 647 609 106 105 14 603 603 118
Fragments arranged by size:
1207 677 647 609 603 603 558 489
467 205 174 140 139 118 106 105
101 82 66 64 52 51 28 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2102 2529 3387 4094 5364 6576 7305
Size: 2102 427 858 707 1270 1212 729
Fragments arranged by size:
2102 1270 1212 858 729 707 427
BtsI GCAGTG_nn'
Cuts at: 0 810 2788 2820 4341 4965 6406 7132 7305
Size: 810 1978 32 1521 624 1441 726 173
Fragments arranged by size:
1978 1521 1441 810 726 624 173 32
DraIII CAC_nnn'GTG
Cuts at: 0 2034 2178 2247 3219 6351 7305
Size: 2034 144 69 972 3132 954
Fragments arranged by size:
3132 2034 972 954 144 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 2710 7039 7305
Size: 2710 4329 266
Fragments arranged by size:
4329 2710 266
EaeI y'GGCC_r
Cuts at: 0 228 461 539 875 1056 1097 1175 1950
Size: 228 233 78 336 181 41 78 775
Cuts at: 1950 2156 2319 2381 2609 2845 3398 4266 4409
Size: 206 163 62 228 236 553 868 143
Cuts at: 4409 5022 5399 5417 5441 5759 5919 5978 6046
Size: 613 377 18 24 318 160 59 68
Cuts at: 6046 6098 6192 6203 6587 6764 7305
Size: 52 94 11 384 177 541
Fragments arranged by size:
868 775 613 553 541 384 377 336
318 236 233 228 228 206 181 177
163 160 143 94 78 78 68 62
59 52 41 24 18 11
EagI C'GGCC_G
Cuts at: 0 1950 7305
Size: 1950 5355
EarI CTCTTCn'nnn_
Cuts at: 0 2365 3622 3877 4375 5053 5638 5740 5785
Size: 2365 1257 255 498 678 585 102 45
Cuts at: 5785 7305
Size: 1520
Fragments arranged by size:
2365 1520 1257 678 585 498 255 102
45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1864 1903 2197 2835 3129 4051 5694 7305
Size: 1864 39 294 638 294 922 1643 1611
Fragments arranged by size:
1864 1643 1611 922 638 294 294 39
EcoICRI GAG'CTC
Cuts at: 0 357 480 855 1301 3333 3750 3819 6423
Size: 357 123 375 446 2032 417 69 2604
Cuts at: 6423 7194 7305
Size: 771 111
Fragments arranged by size:
2604 2032 771 446 417 375 357 123
111 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 657 721 1528 1869 2332 2622 3349
Size: 400 257 64 807 341 463 290 727
Cuts at: 3349 3517 3736 3904 3961 4003 4321 4747 5128
Size: 168 219 168 57 42 318 426 381
Cuts at: 5128 5821 6352 6376 6529 6538 6835 7123 7305
Size: 693 531 24 153 9 297 288 182
Fragments arranged by size:
807 727 693 531 463 426 400 381
341 318 297 290 288 257 219 182
168 168 153 64 57 42 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 1510 1621 1780 4234 5359 6166 7305
Size: 619 891 111 159 2454 1125 807 1139
Fragments arranged by size:
2454 1139 1125 891 807 619 159 111
EcoO109I rG'GnC_Cy
Cuts at: 0 69 363 384 972 1129 3324 3486 3684
Size: 69 294 21 588 157 2195 162 198
Cuts at: 3684 3685 3726 4041 4212 4251 4657 4818 5138
Size: 1 41 315 171 39 406 161 320
Cuts at: 5138 5139 5694 5796 7224 7245 7305
Size: 1 555 102 1428 21 60
Fragments arranged by size:
2195 1428 588 555 406 320 315 294
198 171 162 161 157 102 69 60
41 39 21 21 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 4386 7305
Size: 177 4209 2919
Fragments arranged by size:
4209 2919 177
FseI GG_CCGG'CC
Cuts at: 0 1232 7305
Size: 1232 6073
FspI TGC'GCA
Cuts at: 0 4931 5170 7305
Size: 4931 239 2135
Fragments arranged by size:
4931 2135 239
HaeII r_GCGC'y
Cuts at: 0 178 294 834 940 964 1282 2916 3870
Size: 178 116 540 106 24 318 1634 954
Cuts at: 3870 4536 5336 6666 6751 7305
Size: 666 800 1330 85 554
Fragments arranged by size:
1634 1330 954 800 666 554 540 318
178 116 106 85 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 1000 1032 1618 1650 3961 3993
Size: 592 32 376 32 586 32 2311 32
Cuts at: 3993 4240 4272 4431 4463 6994 7026 7111 7143
Size: 247 32 159 32 2531 32 85 32
Cuts at: 7143 7305
Size: 162
Fragments arranged by size:
2531 2311 592 586 376 247 162 159
85 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1404 1767 5811 6210 6264 7305
Size: 1404 363 4044 399 54 1041
Fragments arranged by size:
4044 1404 1041 399 363 54
KasI G'GCGC_C
Cuts at: 0 174 936 7305
Size: 174 762 6369
Fragments arranged by size:
6369 762 174
KpnI G_GTAC'C
Cuts at: 0 3648 6609 7305
Size: 3648 2961 696
Fragments arranged by size:
3648 2961 696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3151 3362 3944 5081 5091 6944 7305
Size: 3151 211 582 1137 10 1853 361
Fragments arranged by size:
3151 1853 1137 582 361 211 10
MscI TGG'CCA
Cuts at: 0 463 877 1177 2158 2383 3400 4411 5401
Size: 463 414 300 981 225 1017 1011 990
Cuts at: 5401 5761 5921 5980 6100 6766 7305
Size: 360 160 59 120 666 539
Fragments arranged by size:
1017 1011 990 981 666 539 463 414
360 300 225 160 120 59
MslI CAynn'nnrTG
Cuts at: 0 148 2020 2287 2389 2488 2695 3097 3172
Size: 148 1872 267 102 99 207 402 75
Cuts at: 3172 3542 4417 4624 5078 5395 5875 6061 6643
Size: 370 875 207 454 317 480 186 582
Cuts at: 6643 6772 6943 7305
Size: 129 171 362
Fragments arranged by size:
1872 875 582 480 454 402 370 362
317 267 207 207 186 171 148 129
102 99 75
MspA1I CmG'CkG
Cuts at: 0 18 24 172 282 867 894 1144 1329
Size: 18 6 148 110 585 27 250 185
Cuts at: 1329 1668 2124 2277 3732 3740 4458 4679 4855
Size: 339 456 153 1455 8 718 221 176
Cuts at: 4855 4916 5483 5969 6447 6560 7278 7305
Size: 61 567 486 478 113 718 27
Fragments arranged by size:
1455 718 718 585 567 486 478 456
339 250 221 185 176 153 148 113
110 61 27 27 18 8 6
NaeI GCC'GGC
Cuts at: 0 192 1230 1613 2613 2911 5417 5591 6545
Size: 192 1038 383 1000 298 2506 174 954
Cuts at: 6545 7305
Size: 760
Fragments arranged by size:
2506 1038 1000 954 760 383 298 192
174
NarI GG'CG_CC
Cuts at: 0 175 937 7305
Size: 175 762 6368
Fragments arranged by size:
6368 762 175
NcoI C'CATG_G
Cuts at: 0 2219 2900 3585 5147 5981 7305
Size: 2219 681 685 1562 834 1324
Fragments arranged by size:
2219 1562 1324 834 685 681
NgoMIV G'CCGG_C
Cuts at: 0 190 1228 1611 2611 2909 5415 5589 6543
Size: 190 1038 383 1000 298 2506 174 954
Cuts at: 6543 7305
Size: 762
Fragments arranged by size:
2506 1038 1000 954 762 383 298 190
174
NotI GC'GGCC_GC
Cuts at: 0 1950 7305
Size: 1950 5355
NsiI A_TGCA'T
Cuts at: 0 3903 7305
Size: 3903 3402
NspI r_CATG'y
Cuts at: 0 2487 2760 3204 3781 4722 5217 5532 6060
Size: 2487 273 444 577 941 495 315 528
Cuts at: 6060 7305
Size: 1245
Fragments arranged by size:
2487 1245 941 577 528 495 444 315
273
PasI CC'CwG_GG
Cuts at: 0 1041 1305 4030 7305
Size: 1041 264 2725 3275
Fragments arranged by size:
3275 2725 1041 264
PciI A'CATG_T
Cuts at: 0 2483 7305
Size: 2483 4822
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 894 1081 3540 7305
Size: 378 516 187 2459 3765
Fragments arranged by size:
3765 2459 516 378 187
PfoI T'CCnGG_A
Cuts at: 0 698 1505 1985 3434 4012 4433 6181 6472
Size: 698 807 480 1449 578 421 1748 291
Cuts at: 6472 6623 6908 7305
Size: 151 285 397
Fragments arranged by size:
1748 1449 807 698 578 480 421 397
291 285 151
PmlI CAC'GTG
Cuts at: 0 1860 2343 2729 7305
Size: 1860 483 386 4576
Fragments arranged by size:
4576 1860 483 386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3934 3966 7305
Size: 3934 32 3339
Fragments arranged by size:
3934 3339 32
PpuMI rG'GwC_Cy
Cuts at: 0 69 972 3324 3486 4212 4818 5694 5796
Size: 69 903 2352 162 726 606 876 102
Cuts at: 5796 7224 7305
Size: 1428 81
Fragments arranged by size:
2352 1428 903 876 726 606 162 102
81 69
PshAI GACnn'nnGTC
Cuts at: 0 6169 7305
Size: 6169 1136
PspOMI G'GGCC_C
Cuts at: 0 396 1128 1936 3684 3725 4041 4657 5138
Size: 396 732 808 1748 41 316 616 481
Cuts at: 5138 7271 7305
Size: 2133 34
Fragments arranged by size:
2133 1748 808 732 616 481 396 316
41 34
PspXI vC'TCGA_Gb
Cuts at: 0 358 7305
Size: 358 6947
PstI C_TGCA'G
Cuts at: 0 23 257 782 805 959 1001 1170 1196
Size: 23 234 525 23 154 42 169 26
Cuts at: 1196 1238 1526 1547 1664 2795 4118 4826 5363
Size: 42 288 21 117 1131 1323 708 537
Cuts at: 5363 5474 6446 7139 7305
Size: 111 972 693 166
Fragments arranged by size:
1323 1131 972 708 693 537 525 288
234 169 166 154 117 111 42 42
26 23 23 21
PvuII CAG'CTG
Cuts at: 0 18 24 282 867 894 1668 2277 3740
Size: 18 6 258 585 27 774 609 1463
Cuts at: 3740 6447 7278 7305
Size: 2707 831 27
Fragments arranged by size:
2707 1463 831 774 609 585 258 27
27 18 6
SacI G_AGCT'C
Cuts at: 0 359 482 857 1303 3335 3752 3821 6425
Size: 359 123 375 446 2032 417 69 2604
Cuts at: 6425 7196 7305
Size: 771 109
Fragments arranged by size:
2604 2032 771 446 417 375 359 123
109 69
SacII CC_GC'GG
Cuts at: 0 5970 7305
Size: 5970 1335
SalI G'TCGA_C
Cuts at: 0 1402 7305
Size: 1402 5903
SapI GCTCTTCn'nnn_
Cuts at: 0 2365 3622 5053 5785 7305
Size: 2365 1257 1431 732 1520
Fragments arranged by size:
2365 1520 1431 1257 732
SbfI CC_TGCA'GG
Cuts at: 0 782 4826 7305
Size: 782 4044 2479
Fragments arranged by size:
4044 2479 782
ScaI AGT'ACT
Cuts at: 0 2590 5905 7305
Size: 2590 3315 1400
Fragments arranged by size:
3315 2590 1400
SexAI A'CCwGG_T
Cuts at: 0 3050 5540 7305
Size: 3050 2490 1765
Fragments arranged by size:
3050 2490 1765
SfcI C'TryA_G
Cuts at: 0 19 253 778 801 955 997 1166 1192
Size: 19 234 525 23 154 42 169 26
Cuts at: 1192 1234 1522 1543 1660 2791 4114 4822 5359
Size: 42 288 21 117 1131 1323 708 537
Cuts at: 5359 5470 5773 6442 6555 7135 7305
Size: 111 303 669 113 580 170
Fragments arranged by size:
1323 1131 708 669 580 537 525 303
288 234 170 169 154 117 113 111
42 42 26 23 21 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2434 7305
Size: 2434 4871
SfoI GGC'GCC
Cuts at: 0 176 938 7305
Size: 176 762 6367
Fragments arranged by size:
6367 762 176
SmaI CCC'GGG
Cuts at: 0 401 6433 6490 7305
Size: 401 6032 57 815
Fragments arranged by size:
6032 815 401 57
SmlI C'TyrA_G
Cuts at: 0 286 358 1417 2200 3271 3595 3622 3955
Size: 286 72 1059 783 1071 324 27 333
Cuts at: 3955 4630 6922 6958 7305
Size: 675 2292 36 347
Fragments arranged by size:
2292 1071 1059 783 675 347 333 324
286 72 36 27
SphI G_CATG'C
Cuts at: 0 5217 6060 7305
Size: 5217 843 1245
Fragments arranged by size:
5217 1245 843
SrfI GCCC'GGGC
Cuts at: 0 401 7305
Size: 401 6904
StuI AGG'CCT
Cuts at: 0 1786 2893 4828 6331 7305
Size: 1786 1107 1935 1503 974
Fragments arranged by size:
1935 1786 1503 1107 974
StyI C'CwwG_G
Cuts at: 0 645 878 1715 2219 2423 2900 2987 3585
Size: 645 233 837 504 204 477 87 598
Cuts at: 3585 4361 5147 5456 5981 6167 6653 7305
Size: 776 786 309 525 186 486 652
Fragments arranged by size:
837 786 776 652 645 598 525 504
486 477 309 233 204 186 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2235 2765 2965 3547 4507 4603 5026 5160
Size: 2235 530 200 582 960 96 423 134
Cuts at: 5160 5636 6214 7029 7305
Size: 476 578 815 276
Fragments arranged by size:
2235 960 815 582 578 530 476 423
276 200 134 96
TatI w'GTAC_w
Cuts at: 0 2417 2588 3500 5891 5903 6029 6449 7305
Size: 2417 171 912 2391 12 126 420 856
Fragments arranged by size:
2417 2391 912 856 420 171 126 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4279 4311 6335 6367 7305
Size: 4279 32 2024 32 938
Fragments arranged by size:
4279 2024 938 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1408 1798 3366 4098 4222 5959 6145 7305
Size: 1408 390 1568 732 124 1737 186 1160
Fragments arranged by size:
1737 1568 1408 1160 732 390 186 124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 577 1722 2430 2697 3408 3453 3537 4650
Size: 577 1145 708 267 711 45 84 1113
Cuts at: 4650 5343 5853 6642 7305
Size: 693 510 789 663
Fragments arranged by size:
1145 1113 789 711 708 693 663 577
510 267 84 45
XhoI C'TCGA_G
Cuts at: 0 358 7305
Size: 358 6947
XmaI C'CCGG_G
Cuts at: 0 399 6431 6488 7305
Size: 399 6032 57 817
Fragments arranged by size:
6032 817 399 57
ZraI GAC'GTC
Cuts at: 0 185 4173 4874 7305
Size: 185 3988 701 2431
Fragments arranged by size:
3988 2431 701 185
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI ApoI AscI AseI AsiSI
BaeI BmtI BsiWI BsmI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI EcoRI EcoRV FspAI HindIII HpaI
MfeI MluI NdeI NheI NruI PacI PmeI PsiI
PsrI PvuI RsrII SanDI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XmnI