Restriction map for
pF1KA1065
(Linear) (Six-base) MAPSORT of: KIAA1065.seq Check: 3590 from: 1 to: 1923
KIAA1065.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 0 1872 1923
Size: 1872 51
Acc65I G'GTAC_C
Cuts at: 0 1147 1923
Size: 1147 776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 817 1109 1323 1923
Size: 337 480 292 214 600
Fragments arranged by size:
600 480 337 292 214
AflIII A'CryG_T
Cuts at: 0 270 1280 1923
Size: 270 1010 643
Fragments arranged by size:
1010 643 270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 1923
Size: 382 1541
AlwNI CAG_nnn'CTG
Cuts at: 0 315 666 1683 1923
Size: 315 351 1017 240
Fragments arranged by size:
1017 351 315 240
ApaI G_GGCC'C
Cuts at: 0 103 435 636 1036 1066 1543 1840 1923
Size: 103 332 201 400 30 477 297 83
Fragments arranged by size:
477 400 332 297 201 103 83 30
ApoI r'AATT_y
Cuts at: 0 953 1378 1923
Size: 953 425 545
Fragments arranged by size:
953 545 425
AvaI C'yCGr_G
Cuts at: 0 761 1029 1923
Size: 761 268 894
Fragments arranged by size:
894 761 268
BanI G'GyrC_C
Cuts at: 0 1147 1182 1620 1923
Size: 1147 35 438 303
Fragments arranged by size:
1147 438 303 35
BanII G_rGCy'C
Cuts at: 0 103 435 574 636 1036 1058 1066 1337
Size: 103 332 139 62 400 22 8 271
Cuts at: 1337 1543 1711 1840 1923
Size: 206 168 129 83
Fragments arranged by size:
400 332 271 206 168 139 129 103
83 62 22 8
BglI GCCn_nnn'nGGC
Cuts at: 0 719 1642 1923
Size: 719 923 281
Fragments arranged by size:
923 719 281
BlpI GC'TnA_GC
Cuts at: 0 872 1923
Size: 872 1051
Bme1580I G_kGCm'C
Cuts at: 0 103 435 616 636 1036 1066 1187 1515
Size: 103 332 181 20 400 30 121 328
Cuts at: 1515 1543 1840 1923
Size: 28 297 83
Fragments arranged by size:
400 332 328 297 181 121 103 83
30 28 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 1593 1705 1843 1923
Size: 1593 112 138 80
Fragments arranged by size:
1593 138 112 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1923
Size: 993 930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 814 1211 1239 1923
Size: 498 316 397 28 684
Fragments arranged by size:
684 498 397 316 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1479 1923
Size: 385 1094 444
Fragments arranged by size:
1094 444 385
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1923
Size: 538 30 1355
Fragments arranged by size:
1355 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 667 841 1923
Size: 388 279 174 1082
Fragments arranged by size:
1082 388 279 174
BseYI C'CCAG_C
Cuts at: 0 278 617 639 731 1024 1261 1504 1676
Size: 278 339 22 92 293 237 243 172
Cuts at: 1676 1923
Size: 247
Fragments arranged by size:
339 293 278 247 243 237 172 92
22
BsiHKAI G_wGCw'C
Cuts at: 0 1337 1632 1923
Size: 1337 295 291
Fragments arranged by size:
1337 295 291
Bsp1286I G_dGCh'C
Cuts at: 0 103 435 574 616 636 1036 1058 1066
Size: 103 332 139 42 20 400 22 8
Cuts at: 1066 1187 1337 1515 1543 1632 1711 1840 1923
Size: 121 150 178 28 89 79 129 83
Fragments arranged by size:
400 332 178 150 139 129 121 103
89 83 79 42 28 22 20 8
BspHI T'CATG_A
Cuts at: 0 167 1923
Size: 167 1756
BsrDI GCAATG_nn'
Cuts at: 0 1850 1923
Size: 1850 73
BsrGI T'GTAC_A
Cuts at: 0 221 1923
Size: 221 1702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1627 1923
Size: 1627 296
BstEII G'GTnAC_C
Cuts at: 0 1575 1923
Size: 1575 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 817 1444 1767 1897 1923
Size: 480 337 627 323 130 26
Fragments arranged by size:
627 480 337 323 130 26
BstZ17I GTA'TAC
Cuts at: 0 1873 1923
Size: 1873 50
Bsu36I CC'TnA_GG
Cuts at: 0 599 889 1923
Size: 599 290 1034
Fragments arranged by size:
1034 599 290
BtgI C'CryG_G
Cuts at: 0 94 198 588 1037 1174 1760 1784 1923
Size: 94 104 390 449 137 586 24 139
Fragments arranged by size:
586 449 390 139 137 104 94 24
BtsI GCAGTG_nn'
Cuts at: 0 289 1515 1869 1923
Size: 289 1226 354 54
Fragments arranged by size:
1226 354 289 54
DraIII CAC_nnn'GTG
Cuts at: 0 1627 1923
Size: 1627 296
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1923
Size: 9 1914
EaeI y'GGCC_r
Cuts at: 0 1135 1923
Size: 1135 788
EarI CTCTTCn'nnn_
Cuts at: 0 818 1342 1923
Size: 818 524 581
Fragments arranged by size:
818 581 524
EcoICRI GAG'CTC
Cuts at: 0 1335 1923
Size: 1335 588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 498 814 817 1109 1211 1239 1323
Size: 337 161 316 3 292 102 28 84
Cuts at: 1323 1923
Size: 600
Fragments arranged by size:
600 337 316 292 161 102 84 28
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1745 1923
Size: 1745 178
EcoO109I rG'GnC_Cy
Cuts at: 0 99 431 632 633 1033 1062 1325 1540
Size: 99 332 201 1 400 29 263 215
Cuts at: 1540 1837 1923
Size: 297 86
Fragments arranged by size:
400 332 297 263 215 201 99 86
29 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 847 1923
Size: 847 1076
HaeII r_GCGC'y
Cuts at: 0 232 678 1923
Size: 232 446 1245
Fragments arranged by size:
1245 446 232
HincII GTy'rAC
Cuts at: 0 1653 1923
Size: 1653 270
HpaI GTT'AAC
Cuts at: 0 1653 1923
Size: 1653 270
KpnI G_GTAC'C
Cuts at: 0 1151 1923
Size: 1151 772
MluI A'CGCG_T
Cuts at: 0 1280 1923
Size: 1280 643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1923
Size: 983 940
MscI TGG'CCA
Cuts at: 0 1137 1923
Size: 1137 786
MslI CAynn'nnrTG
Cuts at: 0 172 364 478 1765 1923
Size: 172 192 114 1287 158
Fragments arranged by size:
1287 192 172 158 114
MspA1I CmG'CkG
Cuts at: 0 639 837 843 1039 1108 1159 1683 1786
Size: 639 198 6 196 69 51 524 103
Cuts at: 1786 1923
Size: 137
Fragments arranged by size:
639 524 198 196 137 103 69 51
6
NcoI C'CATG_G
Cuts at: 0 198 588 1174 1760 1923
Size: 198 390 586 586 163
Fragments arranged by size:
586 586 390 198 163
NspI r_CATG'y
Cuts at: 0 220 1923
Size: 220 1703
PasI CC'CwG_GG
Cuts at: 0 1058 1094 1229 1923
Size: 1058 36 135 694
Fragments arranged by size:
1058 694 135 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 1094 1144 1923
Size: 1094 50 779
Fragments arranged by size:
1094 779 50
PfoI T'CCnGG_A
Cuts at: 0 71 1923
Size: 71 1852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1552 1584 1923
Size: 34 32 1486 32 339
Fragments arranged by size:
1486 339 34 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1325 1923
Size: 1325 598
PspOMI G'GGCC_C
Cuts at: 0 99 431 632 1032 1062 1539 1836 1923
Size: 99 332 201 400 30 477 297 87
Fragments arranged by size:
477 400 332 297 201 99 87 30
PstI C_TGCA'G
Cuts at: 0 662 842 1923
Size: 662 180 1081
Fragments arranged by size:
1081 662 180
PvuII CAG'CTG
Cuts at: 0 837 843 1159 1923
Size: 837 6 316 764
Fragments arranged by size:
837 764 316 6
SacI G_AGCT'C
Cuts at: 0 1337 1923
Size: 1337 586
SacII CC_GC'GG
Cuts at: 0 1040 1787 1923
Size: 1040 747 136
Fragments arranged by size:
1040 747 136
SapI GCTCTTCn'nnn_
Cuts at: 0 1342 1923
Size: 1342 581
ScaI AGT'ACT
Cuts at: 0 1122 1923
Size: 1122 801
SexAI A'CCwGG_T
Cuts at: 0 1623 1923
Size: 1623 300
SfcI C'TryA_G
Cuts at: 0 658 838 1923
Size: 658 180 1085
Fragments arranged by size:
1085 658 180
SmlI C'TyrA_G
Cuts at: 0 400 1494 1923
Size: 400 1094 429
Fragments arranged by size:
1094 429 400
SpeI A'CTAG_T
Cuts at: 0 814 1923
Size: 814 1109
StuI AGG'CCT
Cuts at: 0 604 1584 1923
Size: 604 980 339
Fragments arranged by size:
980 604 339
StyI C'CwwG_G
Cuts at: 0 198 588 1174 1760 1923
Size: 198 390 586 586 163
Fragments arranged by size:
586 586 390 198 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 353 1923
Size: 136 217 1570
Fragments arranged by size:
1570 217 136
TatI w'GTAC_w
Cuts at: 0 221 329 1120 1923
Size: 221 108 791 803
Fragments arranged by size:
803 791 221 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 1923
Size: 1193 32 698
Fragments arranged by size:
1193 698 32
Tth111I GACn'n_nGTC
Cuts at: 0 9 1923
Size: 9 1914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 472 499 722 1551 1923
Size: 456 16 27 223 829 372
Fragments arranged by size:
829 456 372 223 27 16
XmnI GAAnn'nnTTC
Cuts at: 0 298 322 1923
Size: 298 24 1601
Fragments arranged by size:
1601 298 24
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI BanI BanII BglI BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII HincII HpaI KpnI MluI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI
ScaI SexAI SfcI SmlI SpeI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstYI BtgZI ClaI CspCI
DraI EagI EciI EcoRI EcoRV FseI FspI FspAI
Hin4I HindIII KasI MfeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI SwaI XbaI XhoI XmaI ZraI