Restriction map for
pF1KA1070
(Linear) (Six-base) MAPSORT of: KIAA1070.seq Check: 6641 from: 1 to: 2469
KIAA1070.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 1318 1864 2469
Size: 1318 546 605
Fragments arranged by size:
1318 605 546
Acc65I G'GTAC_C
Cuts at: 0 1827 2469
Size: 1827 642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 848 1298 2469
Size: 97 751 450 1171
Fragments arranged by size:
1171 751 450 97
AflII C'TTAA_G
Cuts at: 0 756 2469
Size: 756 1713
AhdI GACnn_n'nnGTC
Cuts at: 0 2270 2469
Size: 2270 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1508 1540 2469
Size: 1508 32 929
Fragments arranged by size:
1508 929 32
ApoI r'AATT_y
Cuts at: 0 215 567 1180 1248 1632 1681 1923 2469
Size: 215 352 613 68 384 49 242 546
Fragments arranged by size:
613 546 384 352 242 215 68 49
BanI G'GyrC_C
Cuts at: 0 76 1398 1827 2469
Size: 76 1322 429 642
Fragments arranged by size:
1322 642 429 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 2469
Size: 458 2011
BbvCI CC'TCA_GC
Cuts at: 0 2141 2469
Size: 2141 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 591 625 652 686 2469
Size: 591 34 27 34 1783
Fragments arranged by size:
1783 591 34 34 27
Bme1580I G_kGCm'C
Cuts at: 0 81 2469
Size: 81 2388
BmgBI CAC'GTC
Cuts at: 0 1940 2469
Size: 1940 529
BmrI ACTGGGnnnn_n'
Cuts at: 0 1335 1546 2469
Size: 1335 211 923
Fragments arranged by size:
1335 923 211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 2469
Size: 1150 1319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 2423 2469
Size: 275 2148 46
Fragments arranged by size:
2148 275 46
Bpu10I CC'TnA_GC
Cuts at: 0 2141 2469
Size: 2141 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 1655 2469
Size: 672 983 814
Fragments arranged by size:
983 814 672
BsaAI yAC'GTr
Cuts at: 0 20 1448 2469
Size: 20 1428 1021
Fragments arranged by size:
1428 1021 20
BsaBI GATnn'nnATC
Cuts at: 0 613 2219 2469
Size: 613 1606 250
Fragments arranged by size:
1606 613 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1508 1538 1739 1769 2469
Size: 1508 30 201 30 700
Fragments arranged by size:
1508 700 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2124 2313 2469
Size: 2124 189 156
Fragments arranged by size:
2124 189 156
BseYI C'CCAG_C
Cuts at: 0 784 905 2469
Size: 784 121 1564
Fragments arranged by size:
1564 784 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 2469
Size: 1628 841
BsmI GAATG_Cn'
Cuts at: 0 1000 2469
Size: 1000 1469
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1511 2469
Size: 1511 958
Bsp1286I G_dGCh'C
Cuts at: 0 81 2469
Size: 81 2388
BspHI T'CATG_A
Cuts at: 0 2223 2469
Size: 2223 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 812 933 2469
Size: 812 121 1536
Fragments arranged by size:
1536 812 121
BsrDI GCAATG_nn'
Cuts at: 0 609 972 1470 2469
Size: 609 363 498 999
Fragments arranged by size:
999 609 498 363
BstAPI GCAn_nnn'nTGC
Cuts at: 0 934 2469
Size: 934 1535
BstEII G'GTnAC_C
Cuts at: 0 748 832 1648 2303 2469
Size: 748 84 816 655 166
Fragments arranged by size:
816 748 655 166 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 308 2469
Size: 308 2161
BstYI r'GATC_y
Cuts at: 0 775 1419 2469
Size: 775 644 1050
Fragments arranged by size:
1050 775 644
BstZ17I GTA'TAC
Cuts at: 0 1319 1865 2469
Size: 1319 546 604
Fragments arranged by size:
1319 604 546
BtgI C'CryG_G
Cuts at: 0 537 1512 2469
Size: 537 975 957
Fragments arranged by size:
975 957 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1220 2469
Size: 1220 1249
BtsI GCAGTG_nn'
Cuts at: 0 4 50 1952 2053 2469
Size: 4 46 1902 101 416
Fragments arranged by size:
1902 416 101 46 4
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 318 353 939 974 2469
Size: 318 35 586 35 1495
Fragments arranged by size:
1495 586 318 35 35
DraI TTT'AAA
Cuts at: 0 467 2469
Size: 467 2002
EaeI y'GGCC_r
Cuts at: 0 22 120 947 2469
Size: 22 98 827 1522
Fragments arranged by size:
1522 827 98 22
EarI CTCTTCn'nnn_
Cuts at: 0 2174 2469
Size: 2174 295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 275 848 1298 2423 2469
Size: 97 178 573 450 1125 46
Fragments arranged by size:
1125 573 450 178 97 46
EcoO109I rG'GnC_Cy
Cuts at: 0 223 511 2469
Size: 223 288 1958
Fragments arranged by size:
1958 288 223
EcoRV GAT'ATC
Cuts at: 0 318 1273 2469
Size: 318 955 1196
Fragments arranged by size:
1196 955 318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 1765 2469
Size: 406 1359 704
Fragments arranged by size:
1359 704 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 1411 1443 2256 2288 2469
Size: 1201 32 178 32 813 32 181
Fragments arranged by size:
1201 813 181 178 32 32 32
HincII GTy'rAC
Cuts at: 0 624 801 1029 2469
Size: 624 177 228 1440
Fragments arranged by size:
1440 624 228 177
HindIII A'AGCT_T
Cuts at: 0 707 2469
Size: 707 1762
KpnI G_GTAC'C
Cuts at: 0 1831 2469
Size: 1831 638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1799 2025 2469
Size: 820 979 226 444
Fragments arranged by size:
979 820 444 226
MscI TGG'CCA
Cuts at: 0 24 122 949 2469
Size: 24 98 827 1520
Fragments arranged by size:
1520 827 98 24
MslI CAynn'nnrTG
Cuts at: 0 2228 2246 2344 2469
Size: 2228 18 98 125
Fragments arranged by size:
2228 125 98 18
MspA1I CmG'CkG
Cuts at: 0 905 1405 1417 2469
Size: 905 500 12 1052
Fragments arranged by size:
1052 905 500 12
NcoI C'CATG_G
Cuts at: 0 537 2469
Size: 537 1932
NdeI CA'TA_TG
Cuts at: 0 252 432 2469
Size: 252 180 2037
Fragments arranged by size:
2037 252 180
NspI r_CATG'y
Cuts at: 0 56 2469
Size: 56 2413
PmlI CAC'GTG
Cuts at: 0 20 2469
Size: 20 2449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1508 1540 2469
Size: 1508 32 929
Fragments arranged by size:
1508 929 32
PpuMI rG'GwC_Cy
Cuts at: 0 511 2469
Size: 511 1958
PvuII CAG'CTG
Cuts at: 0 905 1405 2469
Size: 905 500 1064
Fragments arranged by size:
1064 905 500
RsrII CG'GwC_CG
Cuts at: 0 2264 2469
Size: 2264 205
SanDI GG'GwC_CC
Cuts at: 0 511 2469
Size: 511 1958
ScaI AGT'ACT
Cuts at: 0 641 2469
Size: 641 1828
SfcI C'TryA_G
Cuts at: 0 1000 1406 1559 2469
Size: 1000 406 153 910
Fragments arranged by size:
1000 910 406 153
SmlI C'TyrA_G
Cuts at: 0 651 756 1670 2469
Size: 651 105 914 799
Fragments arranged by size:
914 799 651 105
SnaBI TAC'GTA
Cuts at: 0 1448 2469
Size: 1448 1021
SpeI A'CTAG_T
Cuts at: 0 721 2469
Size: 721 1748
SphI G_CATG'C
Cuts at: 0 56 2469
Size: 56 2413
StyI C'CwwG_G
Cuts at: 0 537 1164 2469
Size: 537 627 1305
Fragments arranged by size:
1305 627 537
SwaI ATTT'AAAT
Cuts at: 0 467 2469
Size: 467 2002
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 1383 2346 2469
Size: 64 1319 963 123
Fragments arranged by size:
1319 963 123 64
TatI w'GTAC_w
Cuts at: 0 639 655 1533 2090 2469
Size: 639 16 878 557 379
Fragments arranged by size:
878 639 557 379 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1375 1407 2469
Size: 1375 32 1062
Fragments arranged by size:
1375 1062 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 32 305 522 1063 2469
Size: 32 273 217 541 1406
Fragments arranged by size:
1406 541 273 217 32
XmnI GAAnn'nnTTC
Cuts at: 0 1923 2469
Size: 1923 546
Enzymes that do cut:
AccI Acc65I AcuI AflII AhdI AloI ApoI BanI
BbsI BbvCI BcgI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI
NspI PmlI PpiI PpuMI PvuII RsrII SanDI ScaI
SfcI SmlI SnaBI SpeI SphI StyI SwaI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BciVI BclI BfrBI BglI
BglII BlpI BmtI BsaI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI
Bsu36I ClaI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HpaI KasI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI SacI SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SrfI SspI StuI Tth111I XbaI XhoI XmaI ZraI