Restriction map for
pF1KSDA1073
(Linear) (Six-base) MAPSORT of: KIAA1073.seq Check: 3991 from: 1 to: 1929
KIAA1073.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AatII G_ACGT'C
Cuts at: 0 1234 1929
Size: 1234 695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1804 1929
Size: 765 1039 125
Fragments arranged by size:
1039 765 125
AflII C'TTAA_G
Cuts at: 0 1096 1929
Size: 1096 833
AhdI GACnn_n'nnGTC
Cuts at: 0 72 1929
Size: 72 1857
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1031 1063 1929
Size: 1031 32 866
Fragments arranged by size:
1031 866 32
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1929
Size: 127 1802
ApoI r'AATT_y
Cuts at: 0 102 438 477 656 1495 1929
Size: 102 336 39 179 839 434
Fragments arranged by size:
839 434 336 179 102 39
AvaI C'yCGr_G
Cuts at: 0 12 1929
Size: 12 1917
BamHI G'GATC_C
Cuts at: 0 357 1929
Size: 357 1572
BanII G_rGCy'C
Cuts at: 0 13 1197 1724 1929
Size: 13 1184 527 205
Fragments arranged by size:
1184 527 205 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1234 1652 1929
Size: 1234 418 277
Fragments arranged by size:
1234 418 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1409 1443 1929
Size: 1409 34 486
Fragments arranged by size:
1409 486 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 808 1303 1929
Size: 808 495 626
Fragments arranged by size:
808 626 495
BfrBI ATG'CAT
Cuts at: 0 547 1929
Size: 547 1382
BglI GCCn_nnn'nGGC
Cuts at: 0 991 1929
Size: 991 938
BlpI GC'TnA_GC
Cuts at: 0 1274 1929
Size: 1274 655
BmrI ACTGGGnnnn_n'
Cuts at: 0 796 927 1929
Size: 796 131 1002
Fragments arranged by size:
1002 796 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1929
Size: 27 1902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1929
Size: 693 1236
BsaBI GATnn'nnATC
Cuts at: 0 1025 1929
Size: 1025 904
BsaHI Gr'CG_yC
Cuts at: 0 1231 1929
Size: 1231 698
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1093 1123 1929
Size: 1093 30 806
Fragments arranged by size:
1093 806 30
BseYI C'CCAG_C
Cuts at: 0 35 58 1929
Size: 35 23 1871
Fragments arranged by size:
1871 35 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1880 1929
Size: 1880 49
BsiEI CG_ry'CG
Cuts at: 0 55 1929
Size: 55 1874
BsiHKAI G_wGCw'C
Cuts at: 0 13 1724 1929
Size: 13 1711 205
Fragments arranged by size:
1711 205 13
Bsp1286I G_dGCh'C
Cuts at: 0 13 1197 1724 1929
Size: 13 1184 527 205
Fragments arranged by size:
1184 527 205 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 575 1929
Size: 575 1354
BsrDI GCAATG_nn'
Cuts at: 0 1245 1929
Size: 1245 684
BsrFI r'CCGG_y
Cuts at: 0 39 1929
Size: 39 1890
BstYI r'GATC_y
Cuts at: 0 357 517 1929
Size: 357 160 1412
Fragments arranged by size:
1412 357 160
Bsu36I CC'TnA_GG
Cuts at: 0 190 500 1929
Size: 190 310 1429
Fragments arranged by size:
1429 310 190
BtgI C'CryG_G
Cuts at: 0 860 1395 1753 1929
Size: 860 535 358 176
Fragments arranged by size:
860 535 358 176
BtsI GCAGTG_nn'
Cuts at: 0 1257 1929
Size: 1257 672
ClaI AT'CG_AT
Cuts at: 0 1374 1929
Size: 1374 555
DraIII CAC_nnn'GTG
Cuts at: 0 848 1903 1929
Size: 848 1055 26
Fragments arranged by size:
1055 848 26
EaeI y'GGCC_r
Cuts at: 0 52 260 964 1392 1929
Size: 52 208 704 428 537
Fragments arranged by size:
704 537 428 208 52
EagI C'GGCC_G
Cuts at: 0 52 1929
Size: 52 1877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 522 1929
Size: 522 1407
EcoICRI GAG'CTC
Cuts at: 0 11 1722 1929
Size: 11 1711 207
Fragments arranged by size:
1711 207 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 765 1804 1929
Size: 693 72 1039 125
Fragments arranged by size:
1039 693 125 72
EcoO109I rG'GnC_Cy
Cuts at: 0 186 1929
Size: 186 1743
EcoRI G'AATT_C
Cuts at: 0 102 656 1495 1929
Size: 102 554 839 434
Fragments arranged by size:
839 554 434 102
EcoRV GAT'ATC
Cuts at: 0 1056 1929
Size: 1056 873
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1612 1816 1929
Size: 1612 204 113
Fragments arranged by size:
1612 204 113
HaeII r_GCGC'y
Cuts at: 0 299 1929
Size: 299 1630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 782 814 1322 1354 1929
Size: 58 32 692 32 508 32 575
Fragments arranged by size:
692 575 508 58 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1177 1929
Size: 1177 752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1283 1929
Size: 846 437 646
Fragments arranged by size:
846 646 437
MscI TGG'CCA
Cuts at: 0 262 1394 1929
Size: 262 1132 535
Fragments arranged by size:
1132 535 262
MslI CAynn'nnrTG
Cuts at: 0 1250 1297 1415 1929
Size: 1250 47 118 514
Fragments arranged by size:
1250 514 118 47
MspA1I CmG'CkG
Cuts at: 0 1541 1641 1929
Size: 1541 100 288
Fragments arranged by size:
1541 288 100
NaeI GCC'GGC
Cuts at: 0 41 1929
Size: 41 1888
NcoI C'CATG_G
Cuts at: 0 860 1395 1929
Size: 860 535 534
Fragments arranged by size:
860 535 534
NgoMIV G'CCGG_C
Cuts at: 0 39 1929
Size: 39 1890
NsiI A_TGCA'T
Cuts at: 0 549 1929
Size: 549 1380
NspI r_CATG'y
Cuts at: 0 1710 1929
Size: 1710 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 861 1929
Size: 292 569 1068
Fragments arranged by size:
1068 569 292
PfoI T'CCnGG_A
Cuts at: 0 237 1049 1929
Size: 237 812 880
Fragments arranged by size:
880 812 237
PpuMI rG'GwC_Cy
Cuts at: 0 186 1929
Size: 186 1743
PspXI vC'TCGA_Gb
Cuts at: 0 12 1929
Size: 12 1917
PvuII CAG'CTG
Cuts at: 0 1541 1641 1929
Size: 1541 100 288
Fragments arranged by size:
1541 288 100
SacI G_AGCT'C
Cuts at: 0 13 1724 1929
Size: 13 1711 205
Fragments arranged by size:
1711 205 13
SfcI C'TryA_G
Cuts at: 0 1840 1929
Size: 1840 89
SmlI C'TyrA_G
Cuts at: 0 12 1096 1929
Size: 12 1084 833
Fragments arranged by size:
1084 833 12
SpeI A'CTAG_T
Cuts at: 0 1041 1929
Size: 1041 888
SphI G_CATG'C
Cuts at: 0 1710 1929
Size: 1710 219
SspI AAT'ATT
Cuts at: 0 249 747 1065 1929
Size: 249 498 318 864
Fragments arranged by size:
864 498 318 249
StyI C'CwwG_G
Cuts at: 0 384 860 1395 1929
Size: 384 476 535 534
Fragments arranged by size:
535 534 476 384
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 602 1716 1929
Size: 602 1114 213
Fragments arranged by size:
1114 602 213
Tth111I GACn'n_nGTC
Cuts at: 0 1456 1611 1929
Size: 1456 155 318
Fragments arranged by size:
1456 318 155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 668 809 862 1299 1929
Size: 668 141 53 437 630
Fragments arranged by size:
668 630 437 141 53
XhoI C'TCGA_G
Cuts at: 0 12 1929
Size: 12 1917
XmnI GAAnn'nnTTC
Cuts at: 0 1775 1929
Size: 1775 154
ZraI GAC'GTC
Cuts at: 0 1232 1929
Size: 1232 697
Enzymes that do cut:
AatII AcuI AflII AhdI AloI AlwNI ApoI AvaI
BamHI BanII BbsI BcgI BciVI BfrBI BglI BlpI
BmrI BplI BpmI BsaBI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstYI Bsu36I
BtgI BtsI ClaI DraIII EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PflMI PfoI PpuMI PspXI PvuII SacI SfcI
SmlI SpeI SphI SspI StyI TaqII Tth111I XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BclI BglII Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DrdI
EarI EcoNI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI StuI SwaI TatI TstI XbaI
XmaI