Restriction map for
pF1KSDA1075
(Linear) (Six-base) MAPSORT of: KIAA1075.seq Check: 2997 from: 1 to: 4227
KIAA1075.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 479 1171 3171 3242 4227
Size: 479 692 2000 71 985
Fragments arranged by size:
2000 985 692 479 71
AccI GT'mk_AC
Cuts at: 0 1001 1325 4227
Size: 1001 324 2902
Fragments arranged by size:
2902 1001 324
Acc65I G'GTAC_C
Cuts at: 0 2345 2567 4227
Size: 2345 222 1660
Fragments arranged by size:
2345 1660 222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 339 843 2284 3481 4227
Size: 212 127 504 1441 1197 746
Fragments arranged by size:
1441 1197 746 504 212 127
AflIII A'CryG_T
Cuts at: 0 1099 4227
Size: 1099 3128
AhdI GACnn_n'nnGTC
Cuts at: 0 2787 4227
Size: 2787 1440
AleI CACnn'nnGTG
Cuts at: 0 403 1112 3100 4227
Size: 403 709 1988 1127
Fragments arranged by size:
1988 1127 709 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 2252 4227
Size: 358 1894 1975
Fragments arranged by size:
1975 1894 358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2366 2398 3578 3610 4227
Size: 2366 32 1180 32 617
Fragments arranged by size:
2366 1180 617 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 185 774 1033 1606 1620 1987 2393 2817
Size: 185 589 259 573 14 367 406 424
Cuts at: 2817 2954 4227
Size: 137 1273
Fragments arranged by size:
1273 589 573 424 406 367 259 185
137 14
ApaI G_GGCC'C
Cuts at: 0 2650 3271 3623 3874 4227
Size: 2650 621 352 251 353
Fragments arranged by size:
2650 621 353 352 251
ApoI r'AATT_y
Cuts at: 0 796 4227
Size: 796 3431
AvaI C'yCGr_G
Cuts at: 0 1276 1421 2496 2681 3059 3238 3887 4227
Size: 1276 145 1075 185 378 179 649 340
Fragments arranged by size:
1276 1075 649 378 340 185 179 145
AvrII C'CTAG_G
Cuts at: 0 76 1632 4227
Size: 76 1556 2595
Fragments arranged by size:
2595 1556 76
BamHI G'GATC_C
Cuts at: 0 301 1882 4227
Size: 301 1581 2345
Fragments arranged by size:
2345 1581 301
BanI G'GyrC_C
Cuts at: 0 458 1485 1922 2195 2345 2464 2567 2582
Size: 458 1027 437 273 150 119 103 15
Cuts at: 2582 2857 2992 4180 4227
Size: 275 135 1188 47
Fragments arranged by size:
1188 1027 458 437 275 273 150 135
119 103 47 15
BanII G_rGCy'C
Cuts at: 0 39 1802 2650 3010 3154 3271 3623 3659
Size: 39 1763 848 360 144 117 352 36
Cuts at: 3659 3874 4227
Size: 215 353
Fragments arranged by size:
1763 848 360 353 352 215 144 117
39 36
BbeI G_GCGC'C
Cuts at: 0 1926 2996 4184 4227
Size: 1926 1070 1188 43
Fragments arranged by size:
1926 1188 1070 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 1254 4227
Size: 1254 2973
BbvCI CC'TCA_GC
Cuts at: 0 1762 2592 2799 4227
Size: 1762 830 207 1428
Fragments arranged by size:
1762 1428 830 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3040 3074 4227
Size: 3040 34 1153
Fragments arranged by size:
3040 1153 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1751 2462 3396 4227
Size: 1751 711 934 831
Fragments arranged by size:
1751 934 831 711
BclI T'GATC_A
Cuts at: 0 3488 4227
Size: 3488 739
BglI GCCn_nnn'nGGC
Cuts at: 0 238 1470 1787 2324 3062 4227
Size: 238 1232 317 537 738 1165
Fragments arranged by size:
1232 1165 738 537 317 238
BglII A'GATC_T
Cuts at: 0 749 4091 4227
Size: 749 3342 136
Fragments arranged by size:
3342 749 136
BlpI GC'TnA_GC
Cuts at: 0 86 2530 4227
Size: 86 2444 1697
Fragments arranged by size:
2444 1697 86
Bme1580I G_kGCm'C
Cuts at: 0 1126 2198 2294 2320 2469 2585 2650 2660
Size: 1126 1072 96 26 149 116 65 10
Cuts at: 2660 3017 3271 3561 3623 3779 3874 4227
Size: 357 254 290 62 156 95 353
Fragments arranged by size:
1126 1072 357 353 290 254 156 149
116 96 95 65 62 26 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 271 1997 2176 2381 2645 2911 2942 3548
Size: 271 1726 179 205 264 266 31 606
Cuts at: 3548 3626 3644 4222 4227
Size: 78 18 578 5
Fragments arranged by size:
1726 606 578 271 266 264 205 179
78 31 18 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2319 4227
Size: 2319 1908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1031 1081 1254 1478 1867 2056 2146
Size: 113 918 50 173 224 389 189 90
Cuts at: 2146 2159 2417 2644 2658 3755 4020 4227
Size: 13 258 227 14 1097 265 207
Fragments arranged by size:
1097 918 389 265 258 227 224 207
189 173 113 90 50 14 13
Bpu10I CC'TnA_GC
Cuts at: 0 593 1762 2592 2799 2873 2897 3202 4227
Size: 593 1169 830 207 74 24 305 1025
Fragments arranged by size:
1169 1025 830 593 305 207 74 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3517 4034 4227
Size: 3517 517 193
Fragments arranged by size:
3517 517 193
BsaI GGTCTCn'nnnn_
Cuts at: 0 1323 2681 3092 3690 3751 4227
Size: 1323 1358 411 598 61 476
Fragments arranged by size:
1358 1323 598 476 411 61
BsaAI yAC'GTr
Cuts at: 0 405 675 4227
Size: 405 270 3552
Fragments arranged by size:
3552 405 270
BsaHI Gr'CG_yC
Cuts at: 0 1923 2993 4181 4227
Size: 1923 1070 1188 46
Fragments arranged by size:
1923 1188 1070 46
BsaWI w'CCGG_w
Cuts at: 0 116 1130 2860 4055 4227
Size: 116 1014 1730 1195 172
Fragments arranged by size:
1730 1195 1014 172 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2366 2396 2617 2647 2919 2949 3685 3715
Size: 2366 30 221 30 272 30 736 30
Cuts at: 3715 3844 3874 4123 4153 4227
Size: 129 30 249 30 74
Fragments arranged by size:
2366 736 272 249 221 129 74 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 1063 1066 1257 1866 2095 2390 2566
Size: 60 1003 3 191 609 229 295 176
Cuts at: 2566 4227
Size: 1661
Fragments arranged by size:
1661 1003 609 295 229 191 176 60
3
BseYI C'CCAG_C
Cuts at: 0 383 836 1040 1355 1669 2070 2397 2455
Size: 383 453 204 315 314 401 327 58
Cuts at: 2455 2938 3560 3689 4227
Size: 483 622 129 538
Fragments arranged by size:
622 538 483 453 401 383 327 315
314 204 129 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 198 895 1149 1477 2161 2719 4227
Size: 158 40 697 254 328 684 558 1508
Fragments arranged by size:
1508 697 684 558 328 254 158 40
BsiEI CG_ry'CG
Cuts at: 0 1591 1936 4227
Size: 1591 345 2291
Fragments arranged by size:
2291 1591 345
BsiHKAI G_wGCw'C
Cuts at: 0 296 628 923 3154 3793 3836 4227
Size: 296 332 295 2231 639 43 391
Fragments arranged by size:
2231 639 391 332 296 295 43
BsmI GAATG_Cn'
Cuts at: 0 3731 4227
Size: 3731 496
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1672 4227
Size: 1672 2555
Bsp1286I G_dGCh'C
Cuts at: 0 39 296 628 923 1126 1802 2198 2294
Size: 39 257 332 295 203 676 396 96
Cuts at: 2294 2320 2469 2585 2650 2660 3010 3017 3154
Size: 26 149 116 65 10 350 7 137
Cuts at: 3154 3271 3561 3623 3659 3779 3793 3836 3874
Size: 117 290 62 36 120 14 43 38
Cuts at: 3874 4227
Size: 353
Fragments arranged by size:
676 396 353 350 332 295 290 257
203 149 137 120 117 116 96 65
62 43 39 38 36 26 14 10
7
BspEI T'CCGG_A
Cuts at: 0 116 4227
Size: 116 4111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 527 1171 2318 3171 3242 4227
Size: 479 48 644 1147 853 71 985
Fragments arranged by size:
1147 985 853 644 479 71 48
BsrBI CCG'CTC
Cuts at: 0 369 378 4227
Size: 369 9 3849
Fragments arranged by size:
3849 369 9
BsrDI GCAATG_nn'
Cuts at: 0 1022 2572 3390 3449 4227
Size: 1022 1550 818 59 778
Fragments arranged by size:
1550 1022 818 778 59
BsrFI r'CCGG_y
Cuts at: 0 1566 1586 1829 2628 4227
Size: 1566 20 243 799 1599
Fragments arranged by size:
1599 1566 799 243 20
BssSI C'ACGA_G
Cuts at: 0 1138 1387 2293 4227
Size: 1138 249 906 1934
Fragments arranged by size:
1934 1138 906 249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 185 918 2184 2202 4227
Size: 185 733 1266 18 2025
Fragments arranged by size:
2025 1266 733 185 18
BstEII G'GTnAC_C
Cuts at: 0 150 4227
Size: 150 4077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1756 2989 3263 4227
Size: 1756 1233 274 964
Fragments arranged by size:
1756 1233 964 274
BstYI r'GATC_y
Cuts at: 0 301 749 1882 2540 3744 4091 4227
Size: 301 448 1133 658 1204 347 136
Fragments arranged by size:
1204 1133 658 448 347 301 136
Bsu36I CC'TnA_GG
Cuts at: 0 2960 4227
Size: 2960 1267
BtgI C'CryG_G
Cuts at: 0 251 641 1173 1250 1300 2476 3581 4227
Size: 251 390 532 77 50 1176 1105 646
Fragments arranged by size:
1176 1105 646 532 390 251 77 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1100 3269 4227
Size: 1100 2169 958
Fragments arranged by size:
2169 1100 958
BtsI GCAGTG_nn'
Cuts at: 0 1770 2665 2703 4227
Size: 1770 895 38 1524
Fragments arranged by size:
1770 1524 895 38
ClaI AT'CG_AT
Cuts at: 0 158 4227
Size: 158 4069
DraIII CAC_nnn'GTG
Cuts at: 0 918 2184 2315 3556 4227
Size: 918 1266 131 1241 671
Fragments arranged by size:
1266 1241 918 671 131
DrdI GACnn_nn'nnGTC
Cuts at: 0 1315 4227
Size: 1315 2912
EaeI y'GGCC_r
Cuts at: 0 422 776 815 1116 1584 1588 1649 1682
Size: 422 354 39 301 468 4 61 33
Cuts at: 1682 1831 2406 3128 3539 4106 4227
Size: 149 575 722 411 567 121
Fragments arranged by size:
722 575 567 468 422 411 354 301
149 121 61 39 33 4
EagI C'GGCC_G
Cuts at: 0 1588 4227
Size: 1588 2639
EarI CTCTTCn'nnn_
Cuts at: 0 529 910 1694 1912 3749 4000 4227
Size: 529 381 784 218 1837 251 227
Fragments arranged by size:
1837 784 529 381 251 227 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 778 1733 3025 3586 3666 4227
Size: 778 955 1292 561 80 561
Fragments arranged by size:
1292 955 778 561 561 80
EcoICRI GAG'CTC
Cuts at: 0 3152 4227
Size: 3152 1075
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 212 339 843 1031 1081 1254 1478
Size: 113 99 127 504 188 50 173 224
Cuts at: 1478 1867 2056 2146 2159 2284 2417 2644 2658
Size: 389 189 90 13 125 133 227 14
Cuts at: 2658 3481 3755 4020 4227
Size: 823 274 265 207
Fragments arranged by size:
823 504 389 274 265 227 224 207
189 188 173 133 127 125 113 99
90 50 14 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 953 3460 3811 4227
Size: 953 2507 351 416
Fragments arranged by size:
2507 953 416 351
EcoO109I rG'GnC_Cy
Cuts at: 0 1030 1426 1723 2040 2599 2646 2647 2789
Size: 1030 396 297 317 559 47 1 142
Cuts at: 2789 2868 3055 3064 3259 3267 3471 3479 3576
Size: 79 187 9 195 8 204 8 97
Cuts at: 3576 3871 4227
Size: 295 356
Fragments arranged by size:
1030 559 396 356 317 297 295 204
195 187 142 97 79 47 9 8
8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4014 4227
Size: 4014 213
FseI GG_CCGG'CC
Cuts at: 0 1590 4227
Size: 1590 2637
FspI TGC'GCA
Cuts at: 0 3730 4227
Size: 3730 497
HaeII r_GCGC'y
Cuts at: 0 384 1926 2095 2806 2996 4184 4227
Size: 384 1542 169 711 190 1188 43
Fragments arranged by size:
1542 1188 711 384 190 169 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1169 1201 2617 2649 2773 2805 2837 2866
Size: 1169 32 1416 32 124 32 32 29
Cuts at: 2866 2898 2919 2951 3265 3297 3844 3876 4227
Size: 32 21 32 314 32 547 32 351
Fragments arranged by size:
1416 1169 547 351 314 124 32 32
32 32 32 32 32 32 29 21
HincII GTy'rAC
Cuts at: 0 1326 4227
Size: 1326 2901
HindIII A'AGCT_T
Cuts at: 0 709 4227
Size: 709 3518
KasI G'GCGC_C
Cuts at: 0 1922 2992 4180 4227
Size: 1922 1070 1188 47
Fragments arranged by size:
1922 1188 1070 47
KpnI G_GTAC'C
Cuts at: 0 2349 2571 4227
Size: 2349 222 1656
Fragments arranged by size:
2349 1656 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2950 4227
Size: 2950 1277
MscI TGG'CCA
Cuts at: 0 778 817 1651 3130 3541 4108 4227
Size: 778 39 834 1479 411 567 119
Fragments arranged by size:
1479 834 778 567 411 119 39
MslI CAynn'nnrTG
Cuts at: 0 403 886 928 1112 2234 2423 2987 3100
Size: 403 483 42 184 1122 189 564 113
Cuts at: 3100 3784 4227
Size: 684 443
Fragments arranged by size:
1122 684 564 483 443 403 189 184
113 42
MspA1I CmG'CkG
Cuts at: 0 11 456 1461 1603 2202 2258 3591 3796
Size: 11 445 1005 142 599 56 1333 205
Cuts at: 3796 3934 4227
Size: 138 293
Fragments arranged by size:
1333 1005 599 445 293 205 142 138
56 11
NaeI GCC'GGC
Cuts at: 0 1568 1588 4227
Size: 1568 20 2639
Fragments arranged by size:
2639 1568 20
NarI GG'CG_CC
Cuts at: 0 1923 2993 4181 4227
Size: 1923 1070 1188 46
Fragments arranged by size:
1923 1188 1070 46
NcoI C'CATG_G
Cuts at: 0 641 4227
Size: 641 3586
NdeI CA'TA_TG
Cuts at: 0 2403 4227
Size: 2403 1824
NgoMIV G'CCGG_C
Cuts at: 0 1566 1586 4227
Size: 1566 20 2641
Fragments arranged by size:
2641 1566 20
NspI r_CATG'y
Cuts at: 0 604 738 1103 4227
Size: 604 134 365 3124
Fragments arranged by size:
3124 604 365 134
PasI CC'CwG_GG
Cuts at: 0 39 1890 2044 3263 3475 3960 4127 4227
Size: 39 1851 154 1219 212 485 167 100
Fragments arranged by size:
1851 1219 485 212 167 154 100 39
PciI A'CATG_T
Cuts at: 0 1099 4227
Size: 1099 3128
PflMI CCAn_nnn'nTGG
Cuts at: 0 774 1987 2404 2482 2578 2954 3416 3882
Size: 774 1213 417 78 96 376 462 466
Cuts at: 3882 4227
Size: 345
Fragments arranged by size:
1213 774 466 462 417 376 345 96
78
PfoI T'CCnGG_A
Cuts at: 0 101 2033 2433 2635 3398 4227
Size: 101 1932 400 202 763 829
Fragments arranged by size:
1932 829 763 400 202 101
PmlI CAC'GTG
Cuts at: 0 675 4227
Size: 675 3552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1297 1329 2366 2398 4227
Size: 1297 32 1037 32 1829
Fragments arranged by size:
1829 1297 1037 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1426 2040 2599 2789 3259 3471 3576 4227
Size: 1426 614 559 190 470 212 105 651
Fragments arranged by size:
1426 651 614 559 470 212 190 105
PspOMI G'GGCC_C
Cuts at: 0 2646 3267 3619 3870 4227
Size: 2646 621 352 251 357
Fragments arranged by size:
2646 621 357 352 251
PspXI vC'TCGA_Gb
Cuts at: 0 3059 4227
Size: 3059 1168
PstI C_TGCA'G
Cuts at: 0 759 830 2616 3829 3903 4227
Size: 759 71 1786 1213 74 324
Fragments arranged by size:
1786 1213 759 324 74 71
PvuI CG_AT'CG
Cuts at: 0 1936 4227
Size: 1936 2291
PvuII CAG'CTG
Cuts at: 0 1603 2258 3591 3934 4227
Size: 1603 655 1333 343 293
Fragments arranged by size:
1603 1333 655 343 293
SacI G_AGCT'C
Cuts at: 0 3154 4227
Size: 3154 1073
SalI G'TCGA_C
Cuts at: 0 1324 4227
Size: 1324 2903
SanDI GG'GwC_CC
Cuts at: 0 1426 3576 4227
Size: 1426 2150 651
Fragments arranged by size:
2150 1426 651
SapI GCTCTTCn'nnn_
Cuts at: 0 529 1694 4000 4227
Size: 529 1165 2306 227
Fragments arranged by size:
2306 1165 529 227
SexAI A'CCwGG_T
Cuts at: 0 2785 4227
Size: 2785 1442
SfcI C'TryA_G
Cuts at: 0 741 755 826 1598 2099 2253 2612 3825
Size: 741 14 71 772 501 154 359 1213
Cuts at: 3825 3899 3912 4227
Size: 74 13 315
Fragments arranged by size:
1213 772 741 501 359 315 154 74
71 14 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3062 4227
Size: 3062 1165
SfoI GGC'GCC
Cuts at: 0 1924 2994 4182 4227
Size: 1924 1070 1188 45
Fragments arranged by size:
1924 1188 1070 45
SmaI CCC'GGG
Cuts at: 0 1423 2498 3240 3889 4227
Size: 1423 1075 742 649 338
Fragments arranged by size:
1423 1075 742 649 338
SmlI C'TyrA_G
Cuts at: 0 3059 3532 4049 4227
Size: 3059 473 517 178
Fragments arranged by size:
3059 517 473 178
SphI G_CATG'C
Cuts at: 0 604 4227
Size: 604 3623
SrfI GCCC'GGGC
Cuts at: 0 3240 3889 4227
Size: 3240 649 338
Fragments arranged by size:
3240 649 338
StuI AGG'CCT
Cuts at: 0 75 241 1216 1755 1906 2833 4227
Size: 75 166 975 539 151 927 1394
Fragments arranged by size:
1394 975 927 539 166 151 75
StyI C'CwwG_G
Cuts at: 0 76 569 641 1196 1632 2273 3510 3680
Size: 76 493 72 555 436 641 1237 170
Cuts at: 3680 4227
Size: 547
Fragments arranged by size:
1237 641 555 547 493 436 170 76
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1305 2195 2286 2663 3567 4227
Size: 1305 890 91 377 904 660
Fragments arranged by size:
1305 904 890 660 377 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 607 639 1410 1442 2730 2762
Size: 286 32 289 32 771 32 1288 32
Cuts at: 2762 3009 3041 3152 3184 3685 3717 4123 4155
Size: 247 32 111 32 501 32 406 32
Cuts at: 4155 4227
Size: 72
Fragments arranged by size:
1288 771 501 406 289 286 247 111
72 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2819 3858 4227
Size: 2819 1039 369
Fragments arranged by size:
2819 1039 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1352 3517 4209 4227
Size: 15 1337 2165 692 18
Fragments arranged by size:
2165 1337 692 18 15
XhoI C'TCGA_G
Cuts at: 0 3059 4227
Size: 3059 1168
XmaI C'CCGG_G
Cuts at: 0 1421 2496 3238 3887 4227
Size: 1421 1075 742 649 340
Fragments arranged by size:
1421 1075 742 649 340
XmnI GAAnn'nnTTC
Cuts at: 0 112 3994 4227
Size: 112 3882 233
Fragments arranged by size:
3882 233 112
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI ApaLI AscI AseI
AsiSI BaeI BfrBI BmgBI BmtI BsaBI BsiWI BspHI
BsrGI BssHII BstBI BstZ17I CspCI DraI EcoRI EcoRV
FspAI HpaI MfeI MluI NheI NotI NruI NsiI
PacI PmeI PshAI PsiI PsrI RsrII SacII SbfI
ScaI SgfI SgrAI SnaBI SpeI SspI SwaI TatI
XbaI ZraI