Restriction map for
pF1KA1080
(Linear) (Six-base) MAPSORT of: KIAA1080.seq Check: 7788 from: 1 to: 1839
KIAA1080.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 1839
Size: 144 1695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 484 604 1839
Size: 390 94 120 1235
Fragments arranged by size:
1235 390 120 94
AgeI A'CCGG_T
Cuts at: 0 632 1839
Size: 632 1207
AlwNI CAG_nnn'CTG
Cuts at: 0 462 1116 1548 1707 1839
Size: 462 654 432 159 132
Fragments arranged by size:
654 462 432 159 132
ApaI G_GGCC'C
Cuts at: 0 64 1839
Size: 64 1775
ApoI r'AATT_y
Cuts at: 0 141 307 645 884 1248 1493 1839
Size: 141 166 338 239 364 245 346
Fragments arranged by size:
364 346 338 245 239 166 141
AvaI C'yCGr_G
Cuts at: 0 57 610 1839
Size: 57 553 1229
Fragments arranged by size:
1229 553 57
BanI G'GyrC_C
Cuts at: 0 1092 1529 1839
Size: 1092 437 310
Fragments arranged by size:
1092 437 310
BanII G_rGCy'C
Cuts at: 0 64 547 1524 1839
Size: 64 483 977 315
Fragments arranged by size:
977 483 315 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 1039 1839
Size: 437 602 800
Fragments arranged by size:
800 602 437
BclI T'GATC_A
Cuts at: 0 329 1839
Size: 329 1510
BlpI GC'TnA_GC
Cuts at: 0 745 1839
Size: 745 1094
Bme1580I G_kGCm'C
Cuts at: 0 64 1288 1532 1839
Size: 64 1224 244 307
Fragments arranged by size:
1224 307 244 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1589 1839
Size: 1589 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 277 874 984 1185 1334 1670 1839
Size: 97 180 597 110 201 149 336 169
Fragments arranged by size:
597 336 201 180 169 149 110 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1839
Size: 1400 439
BsaWI w'CCGG_w
Cuts at: 0 426 632 839 1478 1839
Size: 426 206 207 639 361
Fragments arranged by size:
639 426 361 207 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1839
Size: 667 30 1142
Fragments arranged by size:
1142 667 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 895 1188 1252 1839
Size: 895 293 64 587
Fragments arranged by size:
895 587 293 64
BseYI C'CCAG_C
Cuts at: 0 1447 1577 1839
Size: 1447 130 262
Fragments arranged by size:
1447 262 130
BsiEI CG_ry'CG
Cuts at: 0 11 1839
Size: 11 1828
BsiHKAI G_wGCw'C
Cuts at: 0 547 1570 1839
Size: 547 1023 269
Fragments arranged by size:
1023 547 269
BsmI GAATG_Cn'
Cuts at: 0 1666 1839
Size: 1666 173
BsmBI CGTCTCn'nnnn_
Cuts at: 0 440 1839
Size: 440 1399
Bsp1286I G_dGCh'C
Cuts at: 0 64 547 1288 1524 1532 1570 1839
Size: 64 483 741 236 8 38 269
Fragments arranged by size:
741 483 269 236 64 38 8
BspEI T'CCGG_A
Cuts at: 0 426 1839
Size: 426 1413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 786 1045 1080 1839
Size: 144 642 259 35 759
Fragments arranged by size:
759 642 259 144 35
BsrFI r'CCGG_y
Cuts at: 0 632 1641 1839
Size: 632 1009 198
Fragments arranged by size:
1009 632 198
BsrGI T'GTAC_A
Cuts at: 0 926 1839
Size: 926 913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 1839
Size: 741 1098
BstEII G'GTnAC_C
Cuts at: 0 951 1839
Size: 951 888
BstXI CCAn_nnnn'nTGG
Cuts at: 0 533 981 1839
Size: 533 448 858
Fragments arranged by size:
858 533 448
BstYI r'GATC_y
Cuts at: 0 209 1839
Size: 209 1630
BtgI C'CryG_G
Cuts at: 0 11 1839
Size: 11 1828
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1752 1787 1839
Size: 1752 35 52
Fragments arranged by size:
1752 52 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 994 1822 1839
Size: 994 828 17
Fragments arranged by size:
994 828 17
EaeI y'GGCC_r
Cuts at: 0 302 1839
Size: 302 1537
EarI CTCTTCn'nnn_
Cuts at: 0 381 409 1839
Size: 381 28 1430
Fragments arranged by size:
1430 381 28
EcoICRI GAG'CTC
Cuts at: 0 545 1839
Size: 545 1294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 277 390 484 604 874 984 1185
Size: 97 180 113 94 120 270 110 201
Cuts at: 1185 1334 1670 1839
Size: 149 336 169
Fragments arranged by size:
336 270 201 180 169 149 120 113
110 97 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1148 1839
Size: 1148 691
EcoO109I rG'GnC_Cy
Cuts at: 0 52 359 615 771 789 1113 1839
Size: 52 307 256 156 18 324 726
Fragments arranged by size:
726 324 307 256 156 52 18
EcoRI G'AATT_C
Cuts at: 0 1493 1839
Size: 1493 346
EcoRV GAT'ATC
Cuts at: 0 1593 1697 1839
Size: 1593 104 142
Fragments arranged by size:
1593 142 104
FspI TGC'GCA
Cuts at: 0 1076 1839
Size: 1076 763
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 667 699 1249 1281 1839
Size: 201 32 434 32 550 32 558
Fragments arranged by size:
558 550 434 201 32 32 32
HindIII A'AGCT_T
Cuts at: 0 577 1657 1839
Size: 577 1080 182
Fragments arranged by size:
1080 577 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 391 1839
Size: 95 296 1448
Fragments arranged by size:
1448 296 95
MscI TGG'CCA
Cuts at: 0 304 1839
Size: 304 1535
MspA1I CmG'CkG
Cuts at: 0 13 1690 1839
Size: 13 1677 149
Fragments arranged by size:
1677 149 13
NaeI GCC'GGC
Cuts at: 0 1643 1839
Size: 1643 196
NgoMIV G'CCGG_C
Cuts at: 0 1641 1839
Size: 1641 198
NspI r_CATG'y
Cuts at: 0 114 187 753 1839
Size: 114 73 566 1086
Fragments arranged by size:
1086 566 114 73
PasI CC'CwG_GG
Cuts at: 0 48 1525 1839
Size: 48 1477 314
Fragments arranged by size:
1477 314 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 1219 1839
Size: 1219 620
PpuMI rG'GwC_Cy
Cuts at: 0 52 359 615 1113 1839
Size: 52 307 256 498 726
Fragments arranged by size:
726 498 307 256 52
PsiI TTA'TAA
Cuts at: 0 481 1839
Size: 481 1358
PspOMI G'GGCC_C
Cuts at: 0 60 1839
Size: 60 1779
PstI C_TGCA'G
Cuts at: 0 737 797 1242 1640 1839
Size: 737 60 445 398 199
Fragments arranged by size:
737 445 398 199 60
PvuII CAG'CTG
Cuts at: 0 1690 1839
Size: 1690 149
SacI G_AGCT'C
Cuts at: 0 547 1839
Size: 547 1292
SacII CC_GC'GG
Cuts at: 0 14 1839
Size: 14 1825
SanDI GG'GwC_CC
Cuts at: 0 52 1839
Size: 52 1787
SbfI CC_TGCA'GG
Cuts at: 0 797 1839
Size: 797 1042
SexAI A'CCwGG_T
Cuts at: 0 1332 1839
Size: 1332 507
SfcI C'TryA_G
Cuts at: 0 733 793 1238 1636 1839
Size: 733 60 445 398 203
Fragments arranged by size:
733 445 398 203 60
SmaI CCC'GGG
Cuts at: 0 59 1839
Size: 59 1780
SmlI C'TyrA_G
Cuts at: 0 1415 1839
Size: 1415 424
StyI C'CwwG_G
Cuts at: 0 912 1124 1319 1767 1839
Size: 912 212 195 448 72
Fragments arranged by size:
912 448 212 195 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1547 1839
Size: 925 622 292
Fragments arranged by size:
925 622 292
TatI w'GTAC_w
Cuts at: 0 926 1839
Size: 926 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 953 985 1839
Size: 953 32 854
Fragments arranged by size:
953 854 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 530 1358 1839
Size: 530 828 481
Fragments arranged by size:
828 530 481
XmaI C'CCGG_G
Cuts at: 0 57 1839
Size: 57 1782
XmnI GAAnn'nnTTC
Cuts at: 0 1486 1807 1839
Size: 1486 321 32
Fragments arranged by size:
1486 321 32
Enzymes that do cut:
AarI AcuI AgeI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BclI BlpI Bme1580I BplI BpmI BpuEI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI BstEII BstXI
BstYI BtgI CspCI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI Hin4I HindIII MmeI
MscI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI
PsiI PspOMI PstI PvuII SacI SacII SanDI SbfI
SexAI SfcI SmaI SmlI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI
BsaHI BsgI BsiWI BspHI BsrBI BsrDI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII
EagI EciI FalI FseI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SalI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
ZraI