Restriction map for
pF1KA1081
(Linear) (Six-base) MAPSORT of: KIAA1081.seq Check: 613 from: 1 to: 2976
KIAA1081.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AatII G_ACGT'C
Cuts at: 0 291 2976
Size: 291 2685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 601 1583 1627 2335 2830 2976
Size: 493 108 982 44 708 495 146
Fragments arranged by size:
982 708 495 493 146 108 44
AflII C'TTAA_G
Cuts at: 0 2002 2842 2976
Size: 2002 840 134
Fragments arranged by size:
2002 840 134
AflIII A'CryG_T
Cuts at: 0 1607 2976
Size: 1607 1369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1481 2976
Size: 1481 1495
AlwNI CAG_nnn'CTG
Cuts at: 0 1221 1338 2631 2976
Size: 1221 117 1293 345
Fragments arranged by size:
1293 1221 345 117
ApaLI G'TGCA_C
Cuts at: 0 2348 2976
Size: 2348 628
ApoI r'AATT_y
Cuts at: 0 1169 1211 1300 1547 2976
Size: 1169 42 89 247 1429
Fragments arranged by size:
1429 1169 247 89 42
AvaI C'yCGr_G
Cuts at: 0 911 2976
Size: 911 2065
BamHI G'GATC_C
Cuts at: 0 710 2976
Size: 710 2266
BanII G_rGCy'C
Cuts at: 0 52 574 597 2976
Size: 52 522 23 2379
Fragments arranged by size:
2379 522 52 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 989 2791 2976
Size: 989 1802 185
Fragments arranged by size:
1802 989 185
BfrBI ATG'CAT
Cuts at: 0 1092 2976
Size: 1092 1884
BglII A'GATC_T
Cuts at: 0 1872 2976
Size: 1872 1104
BlpI GC'TnA_GC
Cuts at: 0 815 2976
Size: 815 2161
Bme1580I G_kGCm'C
Cuts at: 0 15 2352 2976
Size: 15 2337 624
Fragments arranged by size:
2337 624 15
BmtI G_CTAG'C
Cuts at: 0 344 2509 2976
Size: 344 2165 467
Fragments arranged by size:
2165 467 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 679 1604 2719 2976
Size: 588 91 925 1115 257
Fragments arranged by size:
1115 925 588 257 91
Bpu10I CC'TnA_GC
Cuts at: 0 1451 2976
Size: 1451 1525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 2259 2976
Size: 1585 674 717
Fragments arranged by size:
1585 717 674
BsaI GGTCTCn'nnnn_
Cuts at: 0 1190 2976
Size: 1190 1786
BsaBI GATnn'nnATC
Cuts at: 0 682 2976
Size: 682 2294
BsaHI Gr'CG_yC
Cuts at: 0 288 2744 2976
Size: 288 2456 232
Fragments arranged by size:
2456 288 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2755 2785 2923 2953 2976
Size: 2755 30 138 30 23
Fragments arranged by size:
2755 138 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 805 1099 1786 2053 2377 2566 2971 2974
Size: 805 294 687 267 324 189 405 3
Cuts at: 2974 2976
Size: 2
Fragments arranged by size:
805 687 405 324 294 267 189 3
2
BseYI C'CCAG_C
Cuts at: 0 2621 2976
Size: 2621 355
BsiHKAI G_wGCw'C
Cuts at: 0 2352 2976
Size: 2352 624
BsmI GAATG_Cn'
Cuts at: 0 1113 2976
Size: 1113 1863
Bsp1286I G_dGCh'C
Cuts at: 0 15 52 574 597 2352 2976
Size: 15 37 522 23 1755 624
Fragments arranged by size:
1755 624 522 37 23 15
BsrBI CCG'CTC
Cuts at: 0 17 825 1626 1794 2493 2976
Size: 17 808 801 168 699 483
Fragments arranged by size:
808 801 699 483 168 17
BsrGI T'GTAC_A
Cuts at: 0 651 2475 2976
Size: 651 1824 501
Fragments arranged by size:
1824 651 501
BstBI TT'CG_AA
Cuts at: 0 852 2976
Size: 852 2124
BstEII G'GTnAC_C
Cuts at: 0 91 2976
Size: 91 2885
BstXI CCAn_nnnn'nTGG
Cuts at: 0 52 2976
Size: 52 2924
BstYI r'GATC_y
Cuts at: 0 456 710 1872 1944 2976
Size: 456 254 1162 72 1032
Fragments arranged by size:
1162 1032 456 254 72
Bsu36I CC'TnA_GG
Cuts at: 0 1977 2976
Size: 1977 999
BtgI C'CryG_G
Cuts at: 0 2567 2976
Size: 2567 409
BtsI GCAGTG_nn'
Cuts at: 0 138 153 351 1751 2976
Size: 138 15 198 1400 1225
Fragments arranged by size:
1400 1225 198 138 15
DraI TTT'AAA
Cuts at: 0 185 2976
Size: 185 2791
EaeI y'GGCC_r
Cuts at: 0 273 1320 2564 2876 2976
Size: 273 1047 1244 312 100
Fragments arranged by size:
1244 1047 312 273 100
EarI CTCTTCn'nnn_
Cuts at: 0 1199 1505 1559 2338 2441 2723 2976
Size: 1199 306 54 779 103 282 253
Fragments arranged by size:
1199 779 306 282 253 103 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 588 601 679 1583 1604 1627 2335
Size: 493 95 13 78 904 21 23 708
Cuts at: 2335 2719 2830 2976
Size: 384 111 146
Fragments arranged by size:
904 708 493 384 146 111 95 78
23 21 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 1204 2976
Size: 430 774 1772
Fragments arranged by size:
1772 774 430
EcoO109I rG'GnC_Cy
Cuts at: 0 720 1776 2976
Size: 720 1056 1200
Fragments arranged by size:
1200 1056 720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1458 1690 1933 2734 2976
Size: 1458 232 243 801 242
Fragments arranged by size:
1458 801 243 242 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1644 1676 2755 2787 2844 2876 2976
Size: 1644 32 1079 32 57 32 100
Fragments arranged by size:
1644 1079 100 57 32 32 32
HindIII A'AGCT_T
Cuts at: 0 611 931 2976
Size: 611 320 2045
Fragments arranged by size:
2045 611 320
MfeI C'AATT_G
Cuts at: 0 1799 2062 2976
Size: 1799 263 914
Fragments arranged by size:
1799 914 263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1592 2240 2976
Size: 117 1475 648 736
Fragments arranged by size:
1475 736 648 117
MscI TGG'CCA
Cuts at: 0 1322 2566 2976
Size: 1322 1244 410
Fragments arranged by size:
1322 1244 410
MslI CAynn'nnrTG
Cuts at: 0 233 451 676 1426 2976
Size: 233 218 225 750 1550
Fragments arranged by size:
1550 750 233 225 218
MspA1I CmG'CkG
Cuts at: 0 471 732 2976
Size: 471 261 2244
Fragments arranged by size:
2244 471 261
NcoI C'CATG_G
Cuts at: 0 2567 2976
Size: 2567 409
NheI G'CTAG_C
Cuts at: 0 340 2505 2976
Size: 340 2165 471
Fragments arranged by size:
2165 471 340
NsiI A_TGCA'T
Cuts at: 0 1094 2976
Size: 1094 1882
NspI r_CATG'y
Cuts at: 0 675 1611 1960 2976
Size: 675 936 349 1016
Fragments arranged by size:
1016 936 675 349
PasI CC'CwG_GG
Cuts at: 0 52 2976
Size: 52 2924
PciI A'CATG_T
Cuts at: 0 1607 2976
Size: 1607 1369
PfoI T'CCnGG_A
Cuts at: 0 695 2976
Size: 695 2281
PpuMI rG'GwC_Cy
Cuts at: 0 720 2976
Size: 720 2256
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 93 125 2976
Size: 93 32 2851
Fragments arranged by size:
2851 93 32
PstI C_TGCA'G
Cuts at: 0 464 1373 1478 2490 2976
Size: 464 909 105 1012 486
Fragments arranged by size:
1012 909 486 464 105
PvuII CAG'CTG
Cuts at: 0 471 732 2976
Size: 471 261 2244
Fragments arranged by size:
2244 471 261
SapI GCTCTTCn'nnn_
Cuts at: 0 2441 2723 2976
Size: 2441 282 253
Fragments arranged by size:
2441 282 253
ScaI AGT'ACT
Cuts at: 0 1245 2976
Size: 1245 1731
SexAI A'CCwGG_T
Cuts at: 0 2149 2976
Size: 2149 827
SfcI C'TryA_G
Cuts at: 0 460 1369 1474 2410 2486 2976
Size: 460 909 105 936 76 490
Fragments arranged by size:
936 909 490 460 105 76
SmlI C'TyrA_G
Cuts at: 0 1600 2002 2274 2842 2976
Size: 1600 402 272 568 134
Fragments arranged by size:
1600 568 402 272 134
SphI G_CATG'C
Cuts at: 0 1960 2976
Size: 1960 1016
StyI C'CwwG_G
Cuts at: 0 968 2567 2976
Size: 968 1599 409
Fragments arranged by size:
1599 968 409
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 2135 2635 2976
Size: 78 2057 500 341
Fragments arranged by size:
2057 500 341 78
TatI w'GTAC_w
Cuts at: 0 641 651 1243 2475 2976
Size: 641 10 592 1232 501
Fragments arranged by size:
1232 641 592 501 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2890 2922 2923 2955 2976
Size: 2890 32 1 32 21
Fragments arranged by size:
2890 32 32 21 1
XmnI GAAnn'nnTTC
Cuts at: 0 1661 2717 2976
Size: 1661 1056 259
Fragments arranged by size:
1661 1056 259
ZraI GAC'GTC
Cuts at: 0 289 2976
Size: 289 2687
Enzymes that do cut:
AatII AcuI AflII AflIII AlfI AlwNI ApaLI ApoI
AvaI BamHI BanII BbsI BfrBI BglII BlpI Bme1580I
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BsrBI BsrGI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NcoI NheI NsiI NspI
PasI PciI PfoI PpuMI PsrI PstI PvuII SapI
ScaI SexAI SfcI SmlI SphI StyI TaqII TatI
TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BglI BmgBI BmrI
BplI BsaAI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI
NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI