Restriction map for
pF1KSDA1083
(Linear) (Six-base) MAPSORT of: KIAA1083.seq Check: 1799 from: 1 to: 1752
KIAA1083.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 303 1752
Size: 303 1449
AccI GT'mk_AC
Cuts at: 0 1278 1752
Size: 1278 474
AfeI AGC'GCT
Cuts at: 0 241 1752
Size: 241 1511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 1752
Size: 73 1679
AlwNI CAG_nnn'CTG
Cuts at: 0 295 1090 1752
Size: 295 795 662
Fragments arranged by size:
795 662 295
ApoI r'AATT_y
Cuts at: 0 3 849 922 1294 1306 1752
Size: 3 846 73 372 12 446
Fragments arranged by size:
846 446 372 73 12 3
AvaI C'yCGr_G
Cuts at: 0 124 1193 1752
Size: 124 1069 559
Fragments arranged by size:
1069 559 124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1733 1752
Size: 1733 19
BanI G'GyrC_C
Cuts at: 0 43 57 315 321 1752
Size: 43 14 258 6 1431
Fragments arranged by size:
1431 258 43 14 6
BanII G_rGCy'C
Cuts at: 0 218 277 1023 1179 1752
Size: 218 59 746 156 573
Fragments arranged by size:
746 573 218 156 59
BbeI G_GCGC'C
Cuts at: 0 47 319 1752
Size: 47 272 1433
Fragments arranged by size:
1433 272 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 1072 1752
Size: 345 727 680
Fragments arranged by size:
727 680 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 315 1752
Size: 281 34 1437
Fragments arranged by size:
1437 281 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1525 1752
Size: 1525 227
BglI GCCn_nnn'nGGC
Cuts at: 0 66 112 259 686 1752
Size: 66 46 147 427 1066
Fragments arranged by size:
1066 427 147 66 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1585 1752
Size: 1585 167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 1752
Size: 396 1356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1349 1752
Size: 752 597 403
Fragments arranged by size:
752 597 403
BsaAI yAC'GTr
Cuts at: 0 1149 1752
Size: 1149 603
BsaHI Gr'CG_yC
Cuts at: 0 44 316 525 1281 1752
Size: 44 272 209 756 471
Fragments arranged by size:
756 471 272 209 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 1752
Size: 142 30 1580
Fragments arranged by size:
1580 142 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 285 1450 1752
Size: 208 77 1165 302
Fragments arranged by size:
1165 302 208 77
BsiHKAI G_wGCw'C
Cuts at: 0 277 702 1023 1268 1752
Size: 277 425 321 245 484
Fragments arranged by size:
484 425 321 277 245
BsmI GAATG_Cn'
Cuts at: 0 1107 1752
Size: 1107 645
Bsp1286I G_dGCh'C
Cuts at: 0 218 277 702 1023 1179 1268 1752
Size: 218 59 425 321 156 89 484
Fragments arranged by size:
484 425 321 218 156 89 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 303 1752
Size: 303 1449
BsrDI GCAATG_nn'
Cuts at: 0 383 961 1752
Size: 383 578 791
Fragments arranged by size:
791 578 383
BsrFI r'CCGG_y
Cuts at: 0 111 318 1752
Size: 111 207 1434
Fragments arranged by size:
1434 207 111
BsrGI T'GTAC_A
Cuts at: 0 1317 1752
Size: 1317 435
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1090 1513 1567 1752
Size: 1090 423 54 185
Fragments arranged by size:
1090 423 185 54
BstYI r'GATC_y
Cuts at: 0 670 745 1752
Size: 670 75 1007
Fragments arranged by size:
1007 670 75
BtgI C'CryG_G
Cuts at: 0 722 1752
Size: 722 1030
BtsI GCAGTG_nn'
Cuts at: 0 637 1752
Size: 637 1115
ClaI AT'CG_AT
Cuts at: 0 398 1752
Size: 398 1354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1130 1165 1752
Size: 1130 35 587
Fragments arranged by size:
1130 587 35
EaeI y'GGCC_r
Cuts at: 0 560 1752
Size: 560 1192
EarI CTCTTCn'nnn_
Cuts at: 0 226 1248 1752
Size: 226 1022 504
Fragments arranged by size:
1022 504 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 62 110 1752
Size: 62 48 1642
Fragments arranged by size:
1642 62 48
EcoICRI GAG'CTC
Cuts at: 0 275 1021 1752
Size: 275 746 731
Fragments arranged by size:
746 731 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1349 1752
Size: 752 597 403
Fragments arranged by size:
752 597 403
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1028 1752
Size: 1028 724
EcoO109I rG'GnC_Cy
Cuts at: 0 1579 1752
Size: 1579 173
EcoRI G'AATT_C
Cuts at: 0 3 1752
Size: 3 1749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 588 1154 1752
Size: 588 566 598
Fragments arranged by size:
598 588 566
FseI GG_CCGG'CC
Cuts at: 0 115 1752
Size: 115 1637
FspI TGC'GCA
Cuts at: 0 394 1752
Size: 394 1358
HaeII r_GCGC'y
Cuts at: 0 47 243 319 1752
Size: 47 196 76 1433
Fragments arranged by size:
1433 196 76 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1519 1551 1752
Size: 1519 32 201
Fragments arranged by size:
1519 201 32
KasI G'GCGC_C
Cuts at: 0 43 315 1752
Size: 43 272 1437
Fragments arranged by size:
1437 272 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1702 1752
Size: 1702 50
MscI TGG'CCA
Cuts at: 0 562 1752
Size: 562 1190
MspA1I CmG'CkG
Cuts at: 0 168 916 1752
Size: 168 748 836
Fragments arranged by size:
836 748 168
NaeI GCC'GGC
Cuts at: 0 113 1752
Size: 113 1639
NarI GG'CG_CC
Cuts at: 0 44 316 1752
Size: 44 272 1436
Fragments arranged by size:
1436 272 44
NcoI C'CATG_G
Cuts at: 0 722 1752
Size: 722 1030
NgoMIV G'CCGG_C
Cuts at: 0 111 1752
Size: 111 1641
PpuMI rG'GwC_Cy
Cuts at: 0 1579 1752
Size: 1579 173
PsiI TTA'TAA
Cuts at: 0 886 1752
Size: 886 866
PstI C_TGCA'G
Cuts at: 0 678 1095 1752
Size: 678 417 657
Fragments arranged by size:
678 657 417
PvuII CAG'CTG
Cuts at: 0 916 1752
Size: 916 836
SacI G_AGCT'C
Cuts at: 0 277 1023 1752
Size: 277 746 729
Fragments arranged by size:
746 729 277
ScaI AGT'ACT
Cuts at: 0 1343 1752
Size: 1343 409
SfcI C'TryA_G
Cuts at: 0 674 1091 1752
Size: 674 417 661
Fragments arranged by size:
674 661 417
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 686 1752
Size: 686 1066
SfoI GGC'GCC
Cuts at: 0 45 317 1752
Size: 45 272 1435
Fragments arranged by size:
1435 272 45
SgrAI Cr'CCGG_yG
Cuts at: 0 318 1752
Size: 318 1434
SmlI C'TyrA_G
Cuts at: 0 1193 1752
Size: 1193 559
SpeI A'CTAG_T
Cuts at: 0 628 1752
Size: 628 1124
SspI AAT'ATT
Cuts at: 0 1641 1752
Size: 1641 111
StuI AGG'CCT
Cuts at: 0 69 367 680 996 1752
Size: 69 298 313 316 756
Fragments arranged by size:
756 316 313 298 69
StyI C'CwwG_G
Cuts at: 0 563 722 1752
Size: 563 159 1030
Fragments arranged by size:
1030 563 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1342 1752
Size: 1342 410
TatI w'GTAC_w
Cuts at: 0 149 374 1317 1341 1752
Size: 149 225 943 24 411
Fragments arranged by size:
943 411 225 149 24
XbaI T'CTAG_A
Cuts at: 0 582 1752
Size: 582 1170
XhoI C'TCGA_G
Cuts at: 0 1193 1752
Size: 1193 559
Enzymes that do cut:
AarI AccI AfeI AlfI AlwNI ApoI AvaI BaeI
BanI BanII BbeI BbsI BcgI BciVI BglI BmrI
BplI BpmI BsaAI BsaHI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgI
BtsI ClaI CspCI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV
PpuMI PsiI PstI PvuII SacI ScaI SfcI SfiI
SfoI SgrAI SmlI SpeI SspI StuI StyI TaqII
TatI XbaI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbvCI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI DraI DraIII
DrdI EagI EcoRV FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SgfI SmaI SnaBI
SphI SrfI SwaI TstI Tth111I XcmI XmaI XmnI
ZraI