Restriction map for
pF1KA1086
(Linear) (Six-base) MAPSORT of: KIAA1086.seq Check: 6360 from: 1 to: 2697
KIAA1086.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 469 2137 2697
Size: 469 1668 560
Fragments arranged by size:
1668 560 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2042 2697
Size: 2042 655
AfeI AGC'GCT
Cuts at: 0 1052 2697
Size: 1052 1645
AflIII A'CryG_T
Cuts at: 0 500 2697
Size: 500 2197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1134 2697
Size: 1134 1563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2697
Size: 1237 32 1428
Fragments arranged by size:
1428 1237 32
AlwNI CAG_nnn'CTG
Cuts at: 0 172 576 1575 1965 2697
Size: 172 404 999 390 732
Fragments arranged by size:
999 732 404 390 172
ApaI G_GGCC'C
Cuts at: 0 674 1234 1569 1740 2301 2360 2368 2432
Size: 674 560 335 171 561 59 8 64
Cuts at: 2432 2607 2697
Size: 175 90
Fragments arranged by size:
674 561 560 335 175 171 90 64
59 8
AvaI C'yCGr_G
Cuts at: 0 369 406 558 958 988 1038 1621 1829
Size: 369 37 152 400 30 50 583 208
Cuts at: 1829 2176 2219 2368 2431 2473 2697
Size: 347 43 149 63 42 224
Fragments arranged by size:
583 400 369 347 224 208 152 149
63 50 43 42 37 30
BamHI G'GATC_C
Cuts at: 0 898 2442 2697
Size: 898 1544 255
Fragments arranged by size:
1544 898 255
BanI G'GyrC_C
Cuts at: 0 327 477 1352 1457 2697
Size: 327 150 875 105 1240
Fragments arranged by size:
1240 875 327 150 105
BanII G_rGCy'C
Cuts at: 0 110 674 814 985 1234 1409 1422 1569
Size: 110 564 140 171 249 175 13 147
Cuts at: 1569 1700 1740 2057 2164 2301 2360 2368 2432
Size: 131 40 317 107 137 59 8 64
Cuts at: 2432 2438 2480 2607 2693 2697
Size: 6 42 127 86 4
Fragments arranged by size:
564 317 249 175 171 147 140 137
131 127 110 107 86 64 59 42
40 13 8 6 4
BbeI G_GCGC'C
Cuts at: 0 331 2697
Size: 331 2366
BbsI GAAGACnn'nnnn_
Cuts at: 0 793 909 2507 2697
Size: 793 116 1598 190
Fragments arranged by size:
1598 793 190 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1314 1348 2697
Size: 1314 34 1349
Fragments arranged by size:
1349 1314 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 49 367 2697
Size: 49 318 2330
Fragments arranged by size:
2330 318 49
BglI GCCn_nnn'nGGC
Cuts at: 0 293 475 883 1078 1626 2678 2697
Size: 293 182 408 195 548 1052 19
Fragments arranged by size:
1052 548 408 293 195 182 19
BlpI GC'TnA_GC
Cuts at: 0 311 2697
Size: 311 2386
Bme1580I G_kGCm'C
Cuts at: 0 674 1234 1569 1740 2288 2301 2360 2368
Size: 674 560 335 171 548 13 59 8
Cuts at: 2368 2432 2607 2697
Size: 64 175 90
Fragments arranged by size:
674 560 548 335 175 171 90 64
59 13 8
BmgBI CAC'GTC
Cuts at: 0 1010 1539 1656 2505 2697
Size: 1010 529 117 849 192
Fragments arranged by size:
1010 849 529 192 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 946 1122 1572 2697
Size: 946 176 450 1125
Fragments arranged by size:
1125 946 450 176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2028 2124 2697
Size: 2028 96 573
Fragments arranged by size:
2028 573 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 521 667 1453 1498 1537 1600 1963
Size: 202 319 146 786 45 39 63 363
Cuts at: 1963 2413 2422 2697
Size: 450 9 275
Fragments arranged by size:
786 450 363 319 275 202 146 63
45 39 9
Bpu10I CC'TnA_GC
Cuts at: 0 603 1100 2158 2697
Size: 603 497 1058 539
Fragments arranged by size:
1058 603 539 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1726 2697
Size: 1726 971
BsaI GGTCTCn'nnnn_
Cuts at: 0 651 1056 2013 2450 2697
Size: 651 405 957 437 247
Fragments arranged by size:
957 651 437 405 247
BsaAI yAC'GTr
Cuts at: 0 1338 2697
Size: 1338 1359
BsaBI GATnn'nnATC
Cuts at: 0 2251 2697
Size: 2251 446
BsaHI Gr'CG_yC
Cuts at: 0 328 398 875 1636 1844 2697
Size: 328 70 477 761 208 853
Fragments arranged by size:
853 761 477 328 208 70
BsaWI w'CCGG_w
Cuts at: 0 343 1367 2612 2697
Size: 343 1024 1245 85
Fragments arranged by size:
1245 1024 343 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1237 1267 2697
Size: 1237 30 1430
Fragments arranged by size:
1430 1237 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1261 1450 1483 1521 1534 1537 1783 1867
Size: 1261 189 33 38 13 3 246 84
Cuts at: 1867 2001 2031 2065 2143 2659 2697
Size: 134 30 34 78 516 38
Fragments arranged by size:
1261 516 246 189 134 84 78 38
38 34 33 30 13 3
BseYI C'CCAG_C
Cuts at: 0 30 283 643 1024 1408 1568 1958 2353
Size: 30 253 360 381 384 160 390 395
Cuts at: 2353 2519 2598 2697
Size: 166 79 99
Fragments arranged by size:
395 390 384 381 360 253 166 160
99 79 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 817 844 846 910 950 1318 1323
Size: 447 370 27 2 64 40 368 5
Cuts at: 1323 1729 2697
Size: 406 968
Fragments arranged by size:
968 447 406 370 368 64 40 27
5 2
BsiEI CG_ry'CG
Cuts at: 0 1633 1650 2697
Size: 1633 17 1047
Fragments arranged by size:
1633 1047 17
BsiHKAI G_wGCw'C
Cuts at: 0 361 1173 1700 2697
Size: 361 812 527 997
Fragments arranged by size:
997 812 527 361
BsmI GAATG_Cn'
Cuts at: 0 2540 2697
Size: 2540 157
Bsp1286I G_dGCh'C
Cuts at: 0 110 361 674 814 985 1173 1234 1409
Size: 110 251 313 140 171 188 61 175
Cuts at: 1409 1422 1569 1700 1740 2057 2164 2288 2301
Size: 13 147 131 40 317 107 124 13
Cuts at: 2301 2360 2368 2432 2438 2480 2607 2693 2697
Size: 59 8 64 6 42 127 86 4
Fragments arranged by size:
317 313 251 188 175 171 147 140
131 127 124 110 107 86 64 61
59 42 40 13 13 8 6 4
BspEI T'CCGG_A
Cuts at: 0 343 2612 2697
Size: 343 2269 85
Fragments arranged by size:
2269 343 85
BspHI T'CATG_A
Cuts at: 0 425 2697
Size: 425 2272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 469 1340 2055 2137 2697
Size: 469 871 715 82 560
Fragments arranged by size:
871 715 560 469 82
BsrBI CCG'CTC
Cuts at: 0 1473 1734 2496 2697
Size: 1473 261 762 201
Fragments arranged by size:
1473 762 261 201
BsrDI GCAATG_nn'
Cuts at: 0 837 1394 2697
Size: 837 557 1303
Fragments arranged by size:
1303 837 557
BsrFI r'CCGG_y
Cuts at: 0 1224 1355 1632 1649 2276 2594 2672 2697
Size: 1224 131 277 17 627 318 78 25
Fragments arranged by size:
1224 627 318 277 131 78 25 17
BssSI C'ACGA_G
Cuts at: 0 2692 2697
Size: 2692 5
BstAPI GCAn_nnn'nTGC
Cuts at: 0 576 1295 1359 1941 2529 2697
Size: 576 719 64 582 588 168
Fragments arranged by size:
719 588 582 576 168 64
BstEII G'GTnAC_C
Cuts at: 0 1986 2067 2697
Size: 1986 81 630
Fragments arranged by size:
1986 630 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 2697
Size: 129 2568
BstYI r'GATC_y
Cuts at: 0 898 2442 2574 2697
Size: 898 1544 132 123
Fragments arranged by size:
1544 898 132 123
Bsu36I CC'TnA_GG
Cuts at: 0 2135 2263 2697
Size: 2135 128 434
Fragments arranged by size:
2135 434 128
BtgI C'CryG_G
Cuts at: 0 116 128 291 386 1067 1202 1400 1685
Size: 116 12 163 95 681 135 198 285
Cuts at: 1685 2315 2697
Size: 630 382
Fragments arranged by size:
681 630 382 285 198 163 135 116
95 12
BtsI GCAGTG_nn'
Cuts at: 0 1284 2352 2697
Size: 1284 1068 345
Fragments arranged by size:
1284 1068 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 1654 2697
Size: 1654 1043
EaeI y'GGCC_r
Cuts at: 0 72 119 249 971 1055 1630 1703 1763
Size: 72 47 130 722 84 575 73 60
Cuts at: 1763 2186 2402 2697
Size: 423 216 295
Fragments arranged by size:
722 575 423 295 216 130 84 73
72 60 47
EagI C'GGCC_G
Cuts at: 0 1630 2697
Size: 1630 1067
EarI CTCTTCn'nnn_
Cuts at: 0 1787 2611 2697
Size: 1787 824 86
Fragments arranged by size:
1787 824 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 886 1480 2696 2697
Size: 886 594 1216 1
Fragments arranged by size:
1216 886 594 1
EcoICRI GAG'CTC
Cuts at: 0 1698 2697
Size: 1698 999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 521 667 1453 1498 1537 1600 1963
Size: 202 319 146 786 45 39 63 363
Cuts at: 1963 2042 2413 2422 2697
Size: 79 371 9 275
Fragments arranged by size:
786 371 363 319 275 202 146 79
63 45 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1041 1178 1465 2697
Size: 1041 137 287 1232
Fragments arranged by size:
1232 1041 287 137
EcoO109I rG'GnC_Cy
Cuts at: 0 260 562 670 1093 1162 1329 1428 1737
Size: 260 302 108 423 69 167 99 309
Cuts at: 1737 1833 2103 2259 2268 2297 2298 2357 2365
Size: 96 270 156 9 29 1 59 8
Cuts at: 2365 2562 2603 2629 2697
Size: 197 41 26 68
Fragments arranged by size:
423 309 302 270 260 197 167 156
108 99 96 69 68 59 41 29
26 9 8 1
FspI TGC'GCA
Cuts at: 0 1220 2697
Size: 1220 1477
HaeII r_GCGC'y
Cuts at: 0 331 1054 1494 1610 2519 2697
Size: 331 723 440 116 909 178
Fragments arranged by size:
909 723 440 331 178 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1844 1876 2697
Size: 1844 32 821
Fragments arranged by size:
1844 821 32
KasI G'GCGC_C
Cuts at: 0 327 2697
Size: 327 2370
MluI A'CGCG_T
Cuts at: 0 500 2697
Size: 500 2197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1778 2039 2697
Size: 1778 261 658
Fragments arranged by size:
1778 658 261
MscI TGG'CCA
Cuts at: 0 973 1057 2404 2697
Size: 973 84 1347 293
Fragments arranged by size:
1347 973 293 84
MslI CAynn'nnrTG
Cuts at: 0 127 2697
Size: 127 2570
MspA1I CmG'CkG
Cuts at: 0 34 172 517 576 716 834 1204 1455
Size: 34 138 345 59 140 118 370 251
Cuts at: 1455 1485 1965 2228 2697
Size: 30 480 263 469
Fragments arranged by size:
480 469 370 345 263 251 140 138
118 59 34 30
NaeI GCC'GGC
Cuts at: 0 1634 2278 2674 2697
Size: 1634 644 396 23
Fragments arranged by size:
1634 644 396 23
NarI GG'CG_CC
Cuts at: 0 328 2697
Size: 328 2369
NcoI C'CATG_G
Cuts at: 0 128 291 2315 2697
Size: 128 163 2024 382
Fragments arranged by size:
2024 382 163 128
NgoMIV G'CCGG_C
Cuts at: 0 1632 2276 2672 2697
Size: 1632 644 396 25
Fragments arranged by size:
1632 644 396 25
PasI CC'CwG_GG
Cuts at: 0 617 2361 2697
Size: 617 1744 336
Fragments arranged by size:
1744 617 336
PfoI T'CCnGG_A
Cuts at: 0 1164 1430 2390 2438 2697
Size: 1164 266 960 48 259
Fragments arranged by size:
1164 960 266 259 48
PmlI CAC'GTG
Cuts at: 0 1338 2697
Size: 1338 1359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2697
Size: 1237 32 1428
Fragments arranged by size:
1428 1237 32
PpuMI rG'GwC_Cy
Cuts at: 0 260 562 1162 1329 1428 1833 2103 2259
Size: 260 302 600 167 99 405 270 156
Cuts at: 2259 2268 2562 2629 2697
Size: 9 294 67 68
Fragments arranged by size:
600 405 302 294 270 260 167 156
99 68 67 9
PshAI GACnn'nnGTC
Cuts at: 0 397 1831 2697
Size: 397 1434 866
Fragments arranged by size:
1434 866 397
PspOMI G'GGCC_C
Cuts at: 0 670 1230 1565 1736 2297 2356 2364 2428
Size: 670 560 335 171 561 59 8 64
Cuts at: 2428 2603 2697
Size: 175 94
Fragments arranged by size:
670 561 560 335 175 171 94 64
59 8
PspXI vC'TCGA_Gb
Cuts at: 0 958 1038 2176 2219 2473 2697
Size: 958 80 1138 43 254 224
Fragments arranged by size:
1138 958 254 224 80 43
PstI C_TGCA'G
Cuts at: 0 600 1123 1364 2237 2697
Size: 600 523 241 873 460
Fragments arranged by size:
873 600 523 460 241
PvuII CAG'CTG
Cuts at: 0 34 172 576 716 834 1485 1965 2697
Size: 34 138 404 140 118 651 480 732
Fragments arranged by size:
732 651 480 404 140 138 118 34
SacI G_AGCT'C
Cuts at: 0 1700 2697
Size: 1700 997
SacII CC_GC'GG
Cuts at: 0 1205 2697
Size: 1205 1492
SanDI GG'GwC_CC
Cuts at: 0 260 562 1162 2697
Size: 260 302 600 1535
Fragments arranged by size:
1535 600 302 260
SbfI CC_TGCA'GG
Cuts at: 0 600 2697
Size: 600 2097
SfcI C'TryA_G
Cuts at: 0 596 1119 1360 2233 2697
Size: 596 523 241 873 464
Fragments arranged by size:
873 596 523 464 241
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1078 2697
Size: 1078 1619
SfoI GGC'GCC
Cuts at: 0 329 2697
Size: 329 2368
SmaI CCC'GGG
Cuts at: 0 560 990 1623 2370 2433 2697
Size: 560 430 633 747 63 264
Fragments arranged by size:
747 633 560 430 264 63
SmlI C'TyrA_G
Cuts at: 0 958 1038 1741 2176 2219 2473 2697
Size: 958 80 703 435 43 254 224
Fragments arranged by size:
958 703 435 254 224 80 43
SrfI GCCC'GGGC
Cuts at: 0 1623 2433 2697
Size: 1623 810 264
Fragments arranged by size:
1623 810 264
StuI AGG'CCT
Cuts at: 0 1081 1874 2697
Size: 1081 793 823
Fragments arranged by size:
1081 823 793
StyI C'CwwG_G
Cuts at: 0 128 291 662 1179 2315 2697
Size: 128 163 371 517 1136 382
Fragments arranged by size:
1136 517 382 371 163 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2005 2697
Size: 2005 692
TatI w'GTAC_w
Cuts at: 0 509 2697
Size: 509 2188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1065 2322 2595 2697
Size: 1065 1257 273 102
Fragments arranged by size:
1257 1065 273 102
XhoI C'TCGA_G
Cuts at: 0 958 1038 2176 2219 2473 2697
Size: 958 80 1138 43 254 224
Fragments arranged by size:
1138 958 254 224 80 43
XmaI C'CCGG_G
Cuts at: 0 558 988 1621 2368 2431 2697
Size: 558 430 633 747 63 266
Fragments arranged by size:
747 633 558 430 266 63
XmnI GAAnn'nnTTC
Cuts at: 0 916 2697
Size: 916 1781
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SbfI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BclI BfrBI BglII BmtI BsiWI BsmBI BsrGI BssHII
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PsiI PsrI PvuI RsrII SalI
SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TstI Tth111I XbaI ZraI