Restriction map for
pF1KSDA1088
(Linear) (Six-base) MAPSORT of: KIAA1088.seq Check: 6102 from: 1 to: 4200
KIAA1088.orf
With 182 enzymes: SgfI *
February 1, 2012 16:00 ..
AatII G_ACGT'C
Cuts at: 0 833 4200
Size: 833 3367
AccI GT'mk_AC
Cuts at: 0 32 4200
Size: 32 4168
Acc65I G'GTAC_C
Cuts at: 0 2045 4200
Size: 2045 2155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 745 803 973 1079 1356 1774 2416
Size: 664 81 58 170 106 277 418 642
Cuts at: 2416 3802 4200
Size: 1386 398
Fragments arranged by size:
1386 664 642 418 398 277 170 106
81 58
AfeI AGC'GCT
Cuts at: 0 3245 3379 3790 4166 4200
Size: 3245 134 411 376 34
Fragments arranged by size:
3245 411 376 134 34
AflIII A'CryG_T
Cuts at: 0 4112 4200
Size: 4112 88
AhdI GACnn_n'nnGTC
Cuts at: 0 829 4200
Size: 829 3371
AleI CACnn'nnGTG
Cuts at: 0 3390 4076 4200
Size: 3390 686 124
Fragments arranged by size:
3390 686 124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3373 4094 4200
Size: 3373 721 106
Fragments arranged by size:
3373 721 106
AlwNI CAG_nnn'CTG
Cuts at: 0 558 846 1125 1173 1986 2476 2754 3045
Size: 558 288 279 48 813 490 278 291
Cuts at: 3045 3574 3643 4200
Size: 529 69 557
Fragments arranged by size:
813 558 557 529 490 291 288 279
278 69 48
ApaI G_GGCC'C
Cuts at: 0 1725 3210 3710 4200
Size: 1725 1485 500 490
Fragments arranged by size:
1725 1485 500 490
ApaLI G'TGCA_C
Cuts at: 0 159 1267 2669 3393 4200
Size: 159 1108 1402 724 807
Fragments arranged by size:
1402 1108 807 724 159
AvaI C'yCGr_G
Cuts at: 0 419 1961 2282 2434 3420 4200
Size: 419 1542 321 152 986 780
Fragments arranged by size:
1542 986 780 419 321 152
BamHI G'GATC_C
Cuts at: 0 3006 4200
Size: 3006 1194
BanI G'GyrC_C
Cuts at: 0 1653 1846 2045 2099 3000 3756 3812 3976
Size: 1653 193 199 54 901 756 56 164
Cuts at: 3976 4070 4200
Size: 94 130
Fragments arranged by size:
1653 901 756 199 193 164 130 94
56 54
BanII G_rGCy'C
Cuts at: 0 127 418 884 1569 1579 1667 1725 1775
Size: 127 291 466 685 10 88 58 50
Cuts at: 1775 2166 2542 2660 3210 3489 3699 3710 3896
Size: 391 376 118 550 279 210 11 186
Cuts at: 3896 4200
Size: 304
Fragments arranged by size:
685 550 466 391 376 304 291 279
210 186 127 118 88 58 50 11
10
BbeI G_GCGC'C
Cuts at: 0 1850 3004 4200
Size: 1850 1154 1196
Fragments arranged by size:
1850 1196 1154
BbsI GAAGACnn'nnnn_
Cuts at: 0 148 944 2115 3523 3532 3873 4200
Size: 148 796 1171 1408 9 341 327
Fragments arranged by size:
1408 1171 796 341 327 148 9
BbvCI CC'TCA_GC
Cuts at: 0 2819 4200
Size: 2819 1381
BciVI GTATCCnnnnn_n'
Cuts at: 0 2914 4200
Size: 2914 1286
BglI GCCn_nnn'nGGC
Cuts at: 0 223 1662 2284 2496 2791 3190 3205 4068
Size: 223 1439 622 212 295 399 15 863
Cuts at: 4068 4200
Size: 132
Fragments arranged by size:
1439 863 622 399 295 223 212 132
15
BglII A'GATC_T
Cuts at: 0 1529 3539 4200
Size: 1529 2010 661
Fragments arranged by size:
2010 1529 661
BlpI GC'TnA_GC
Cuts at: 0 1304 1357 4200
Size: 1304 53 2843
Fragments arranged by size:
2843 1304 53
Bme1580I G_kGCm'C
Cuts at: 0 163 622 1271 1658 1725 2673 2970 3149
Size: 163 459 649 387 67 948 297 179
Cuts at: 3149 3210 3397 3710 3761 3772 3838 4064 4200
Size: 61 187 313 51 11 66 226 136
Fragments arranged by size:
948 649 459 387 313 297 226 187
179 163 136 67 66 61 51 11
BmgBI CAC'GTC
Cuts at: 0 1135 2193 2232 3392 3753 4113 4200
Size: 1135 1058 39 1160 361 360 87
Fragments arranged by size:
1160 1135 1058 361 360 87 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1483 1563 2232 2291 4051 4200
Size: 1483 80 669 59 1760 149
Fragments arranged by size:
1760 1483 669 149 80 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 202 1319 4200
Size: 202 1117 2881
Fragments arranged by size:
2881 1117 202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 308 321 568 868 994 1025 1160
Size: 139 169 13 247 300 126 31 135
Cuts at: 1160 1341 1489 1519 1729 2533 3161 3840 3938
Size: 181 148 30 210 804 628 679 98
Cuts at: 3938 4181 4200
Size: 243 19
Fragments arranged by size:
804 679 628 300 247 243 210 181
169 148 139 135 126 98 31 30
19 13
Bpu10I CC'TnA_GC
Cuts at: 0 2819 2939 4200
Size: 2819 120 1261
Fragments arranged by size:
2819 1261 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1957 4200
Size: 1957 2243
BsaI GGTCTCn'nnnn_
Cuts at: 0 284 1792 3104 4200
Size: 284 1508 1312 1096
Fragments arranged by size:
1508 1312 1096 284
BsaBI GATnn'nnATC
Cuts at: 0 1477 4200
Size: 1477 2723
BsaHI Gr'CG_yC
Cuts at: 0 830 1412 1847 2059 3001 4200
Size: 830 582 435 212 942 1199
Fragments arranged by size:
1199 942 830 582 435 212
BsaWI w'CCGG_w
Cuts at: 0 1292 4200
Size: 1292 2908
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 757 787 2551 2581 3681 3711
Size: 415 30 312 30 1764 30 1100 30
Cuts at: 3711 3838 3868 4200
Size: 127 30 332
Fragments arranged by size:
1764 1100 415 332 312 127 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1618 2563 2630 2668 2908 3463 4200
Size: 865 753 945 67 38 240 555 737
Fragments arranged by size:
945 865 753 737 555 240 67 38
BseYI C'CCAG_C
Cuts at: 0 411 910 1233 1242 2014 2389 3038 3604
Size: 411 499 323 9 772 375 649 566
Cuts at: 3604 3676 3837 4200
Size: 72 161 363
Fragments arranged by size:
772 649 566 499 411 375 363 323
161 72 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3322 4100 4200
Size: 3322 778 100
Fragments arranged by size:
3322 778 100
BsiEI CG_ry'CG
Cuts at: 0 1160 3411 4088 4200
Size: 1160 2251 677 112
Fragments arranged by size:
2251 1160 677 112
BsiHKAI G_wGCw'C
Cuts at: 0 163 711 1271 1327 1579 2519 2673 2945
Size: 163 548 560 56 252 940 154 272
Cuts at: 2945 3062 3397 3699 4200
Size: 117 335 302 501
Fragments arranged by size:
940 560 548 501 335 302 272 252
163 154 117 56
BsmI GAATG_Cn'
Cuts at: 0 2948 4200
Size: 2948 1252
BsmBI CGTCTCn'nnnn_
Cuts at: 0 185 1001 2222 4200
Size: 185 816 1221 1978
Fragments arranged by size:
1978 1221 816 185
Bsp1286I G_dGCh'C
Cuts at: 0 127 163 418 622 711 884 1271 1327
Size: 127 36 255 204 89 173 387 56
Cuts at: 1327 1569 1579 1658 1667 1725 1775 2166 2519
Size: 242 10 79 9 58 50 391 353
Cuts at: 2519 2542 2660 2673 2945 2970 3062 3149 3210
Size: 23 118 13 272 25 92 87 61
Cuts at: 3210 3397 3489 3699 3710 3761 3772 3838 3896
Size: 187 92 210 11 51 11 66 58
Cuts at: 3896 4064 4200
Size: 168 136
Fragments arranged by size:
391 387 353 272 255 242 210 204
187 173 168 136 127 118 92 92
89 87 79 66 61 58 58 56
51 50 36 25 23 13 11 11
10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2387 2849 3589 3867 4200
Size: 2387 462 740 278 333
Fragments arranged by size:
2387 740 462 333 278
BsrBI CCG'CTC
Cuts at: 0 599 1418 4042 4200
Size: 599 819 2624 158
Fragments arranged by size:
2624 819 599 158
BsrDI GCAATG_nn'
Cuts at: 0 941 4200
Size: 941 3259
BsrFI r'CCGG_y
Cuts at: 0 392 1843 2048 2274 2598 2973 3406 3410
Size: 392 1451 205 226 324 375 433 4
Cuts at: 3410 3973 3979 4200
Size: 563 6 221
Fragments arranged by size:
1451 563 433 392 375 324 226 221
205 6 4
BssSI C'ACGA_G
Cuts at: 0 1393 4200
Size: 1393 2807
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1135 1825 2841 4200
Size: 1135 690 1016 1359
Fragments arranged by size:
1359 1135 1016 690
BstEII G'GTnAC_C
Cuts at: 0 1288 2414 4200
Size: 1288 1126 1786
Fragments arranged by size:
1786 1288 1126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 1743 1857 3739 4200
Size: 84 1659 114 1882 461
Fragments arranged by size:
1882 1659 461 114 84
BstYI r'GATC_y
Cuts at: 0 476 1478 1529 2630 3006 3539 4200
Size: 476 1002 51 1101 376 533 661
Fragments arranged by size:
1101 1002 661 533 476 376 51
Bsu36I CC'TnA_GG
Cuts at: 0 398 443 2409 3615 4200
Size: 398 45 1966 1206 585
Fragments arranged by size:
1966 1206 585 398 45
BtgI C'CryG_G
Cuts at: 0 1914 2660 3026 3218 3627 3689 4200
Size: 1914 746 366 192 409 62 511
Fragments arranged by size:
1914 746 511 409 366 192 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1626 3657 4200
Size: 1626 2031 543
Fragments arranged by size:
2031 1626 543
BtsI GCAGTG_nn'
Cuts at: 0 1328 3308 4200
Size: 1328 1980 892
Fragments arranged by size:
1980 1328 892
ClaI AT'CG_AT
Cuts at: 0 986 4200
Size: 986 3214
DraIII CAC_nnn'GTG
Cuts at: 0 615 762 2299 3177 4113 4200
Size: 615 147 1537 878 936 87
Fragments arranged by size:
1537 936 878 615 147 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 1018 2383 4200
Size: 1018 1365 1817
Fragments arranged by size:
1817 1365 1018
EaeI y'GGCC_r
Cuts at: 0 560 1157 1380 1733 1911 2214 2504 2702
Size: 560 597 223 353 178 303 290 198
Cuts at: 2702 2792 3296 3408 3886 4085 4200
Size: 90 504 112 478 199 115
Fragments arranged by size:
597 560 504 478 353 303 290 223
199 198 178 115 112 90
EagI C'GGCC_G
Cuts at: 0 1157 3408 4085 4200
Size: 1157 2251 677 115
Fragments arranged by size:
2251 1157 677 115
EarI CTCTTCn'nnn_
Cuts at: 0 1696 2587 2698 4200
Size: 1696 891 111 1502
Fragments arranged by size:
1696 1502 891 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1192 1393 4200
Size: 1192 201 2807
Fragments arranged by size:
2807 1192 201
EcoICRI GAG'CTC
Cuts at: 0 1577 3697 4200
Size: 1577 2120 503
Fragments arranged by size:
2120 1577 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 308 321 568 664 745 803 868
Size: 139 169 13 247 96 81 58 65
Cuts at: 868 973 994 1025 1079 1160 1341 1356 1489
Size: 105 21 31 54 81 181 15 133
Cuts at: 1489 1519 1729 1774 2416 2533 3161 3802 3840
Size: 30 210 45 642 117 628 641 38
Cuts at: 3840 3938 4181 4200
Size: 98 243 19
Fragments arranged by size:
642 641 628 247 243 210 181 169
139 133 117 105 98 96 81 81
65 58 54 45 38 31 30 21
19 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 550 1624 3067 3277 4200
Size: 550 1074 1443 210 923
Fragments arranged by size:
1443 1074 923 550 210
EcoO109I rG'GnC_Cy
Cuts at: 0 81 341 1247 1555 2405 2430 2751 2823
Size: 81 260 906 308 850 25 321 72
Cuts at: 2823 3077 3206 3207 3439 3499 3601 3619 3707
Size: 254 129 1 232 60 102 18 88
Cuts at: 3707 4200
Size: 493
Fragments arranged by size:
906 850 493 321 308 260 254 232
129 102 88 81 72 60 25 18
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 253 3396 4200
Size: 253 3143 804
Fragments arranged by size:
3143 804 253
FseI GG_CCGG'CC
Cuts at: 0 3414 4200
Size: 3414 786
HaeII r_GCGC'y
Cuts at: 0 1753 1850 3004 3185 3247 3381 3792 3917
Size: 1753 97 1154 181 62 134 411 125
Cuts at: 3917 3959 4168 4200
Size: 42 209 32
Fragments arranged by size:
1753 1154 411 209 181 134 125 97
62 42 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 184 214 216 453 485 815 847
Size: 182 2 30 2 237 32 330 32
Cuts at: 847 1597 1629 2317 2349 4200
Size: 750 32 688 32 1851
Fragments arranged by size:
1851 750 688 330 237 182 32 32
32 32 30 2 2
KasI G'GCGC_C
Cuts at: 0 1846 3000 4200
Size: 1846 1154 1200
Fragments arranged by size:
1846 1200 1154
KpnI G_GTAC'C
Cuts at: 0 2049 4200
Size: 2049 2151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 4200
Size: 668 3532
MscI TGG'CCA
Cuts at: 0 562 1735 2216 3888 4200
Size: 562 1173 481 1672 312
Fragments arranged by size:
1672 1173 562 481 312
MslI CAynn'nnrTG
Cuts at: 0 667 1280 3355 3390 3645 4076 4200
Size: 667 613 2075 35 255 431 124
Fragments arranged by size:
2075 667 613 431 255 124 35
MspA1I CmG'CkG
Cuts at: 0 429 1016 1308 1436 1761 2422 2529 2564
Size: 429 587 292 128 325 661 107 35
Cuts at: 2564 2910 2964 3045 3220 4084 4200
Size: 346 54 81 175 864 116
Fragments arranged by size:
864 661 587 429 346 325 292 175
128 116 107 81 54 35
NaeI GCC'GGC
Cuts at: 0 2600 3412 3981 4200
Size: 2600 812 569 219
Fragments arranged by size:
2600 812 569 219
NarI GG'CG_CC
Cuts at: 0 1847 3001 4200
Size: 1847 1154 1199
Fragments arranged by size:
1847 1199 1154
NgoMIV G'CCGG_C
Cuts at: 0 2598 3410 3979 4200
Size: 2598 812 569 221
Fragments arranged by size:
2598 812 569 221
NspI r_CATG'y
Cuts at: 0 1392 2386 3354 4200
Size: 1392 994 968 846
Fragments arranged by size:
1392 994 968 846
PasI CC'CwG_GG
Cuts at: 0 916 1620 1834 2492 3210 4200
Size: 916 704 214 658 718 990
Fragments arranged by size:
990 916 718 704 658 214
PflMI CCAn_nnn'nTGG
Cuts at: 0 813 846 1125 3045 3237 4200
Size: 813 33 279 1920 192 963
Fragments arranged by size:
1920 963 813 279 192 33
PfoI T'CCnGG_A
Cuts at: 0 83 116 242 337 572 634 1094 1985
Size: 83 33 126 95 235 62 460 891
Cuts at: 1985 2978 3856 4200
Size: 993 878 344
Fragments arranged by size:
993 891 878 460 344 235 126 95
83 62 33
PpuMI rG'GwC_Cy
Cuts at: 0 81 341 2405 2430 2823 3439 3601 3619
Size: 81 260 2064 25 393 616 162 18
Cuts at: 3619 4200
Size: 581
Fragments arranged by size:
2064 616 581 393 260 162 81 25
18
PshAI GACnn'nnGTC
Cuts at: 0 79 4200
Size: 79 4121
PspOMI G'GGCC_C
Cuts at: 0 1721 3206 3706 4200
Size: 1721 1485 500 494
Fragments arranged by size:
1721 1485 500 494
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 373 405 4200
Size: 373 32 3795
Fragments arranged by size:
3795 373 32
PstI C_TGCA'G
Cuts at: 0 98 1169 1335 2759 3036 3639 4200
Size: 98 1071 166 1424 277 603 561
Fragments arranged by size:
1424 1071 603 561 277 166 98
PvuII CAG'CTG
Cuts at: 0 429 2422 2564 2910 3045 4200
Size: 429 1993 142 346 135 1155
Fragments arranged by size:
1993 1155 429 346 142 135
RsrII CG'GwC_CG
Cuts at: 0 3509 4200
Size: 3509 691
SacI G_AGCT'C
Cuts at: 0 1579 3699 4200
Size: 1579 2120 501
Fragments arranged by size:
2120 1579 501
SacII CC_GC'GG
Cuts at: 0 3221 4200
Size: 3221 979
SanDI GG'GwC_CC
Cuts at: 0 2430 3439 4200
Size: 2430 1009 761
Fragments arranged by size:
2430 1009 761
SapI GCTCTTCn'nnn_
Cuts at: 0 1696 2698 4200
Size: 1696 1002 1502
Fragments arranged by size:
1696 1502 1002
SbfI CC_TGCA'GG
Cuts at: 0 2759 4200
Size: 2759 1441
SexAI A'CCwGG_T
Cuts at: 0 839 4200
Size: 839 3361
SfcI C'TryA_G
Cuts at: 0 94 472 1165 1331 2288 2755 3032 3225
Size: 94 378 693 166 957 467 277 193
Cuts at: 3225 3635 4200
Size: 410 565
Fragments arranged by size:
957 693 565 467 410 378 277 193
166 94
SfoI GGC'GCC
Cuts at: 0 1848 3002 4200
Size: 1848 1154 1198
Fragments arranged by size:
1848 1198 1154
SgrAI Cr'CCGG_yG
Cuts at: 0 1843 4200
Size: 1843 2357
SmaI CCC'GGG
Cuts at: 0 421 1963 2284 3422 4200
Size: 421 1542 321 1138 778
Fragments arranged by size:
1542 1138 778 421 321
SmlI C'TyrA_G
Cuts at: 0 1972 4200
Size: 1972 2228
SphI G_CATG'C
Cuts at: 0 1392 4200
Size: 1392 2808
StuI AGG'CCT
Cuts at: 0 3823 3930 4200
Size: 3823 107 270
Fragments arranged by size:
3823 270 107
StyI C'CwwG_G
Cuts at: 0 77 860 1082 2847 4200
Size: 77 783 222 1765 1353
Fragments arranged by size:
1765 1353 783 222 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1524 2169 2310 2761 3140 4021 4200
Size: 1524 645 141 451 379 881 179
Fragments arranged by size:
1524 881 645 451 379 179 141
TatI w'GTAC_w
Cuts at: 0 68 4200
Size: 68 4132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 757 789 2551 2583 2960 2992
Size: 415 32 310 32 1762 32 377 32
Cuts at: 2992 3681 3713 3838 3870 4200
Size: 689 32 125 32 330
Fragments arranged by size:
1762 689 415 377 330 310 125 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 3021 4200
Size: 589 2432 1179
Fragments arranged by size:
2432 1179 589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1282 1440 1887 2011 2029 2499 2694
Size: 570 712 158 447 124 18 470 195
Cuts at: 2694 3913 4200
Size: 1219 287
Fragments arranged by size:
1219 712 570 470 447 287 195 158
124 18
XmaI C'CCGG_G
Cuts at: 0 419 1961 2282 3420 4200
Size: 419 1542 321 1138 780
Fragments arranged by size:
1542 1138 780 419 321
ZraI GAC'GTC
Cuts at: 0 831 4200
Size: 831 3369
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PflMI PfoI PpuMI PshAI PspOMI PsrI
PstI PvuII RsrII SacI SacII SanDI SapI SbfI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BcgI BclI BfrBI BmtI BsaAI
BsiWI BspEI BspHI BsrGI BssHII BstBI BstZ17I CspCI
DraI EcoRI EcoRV FspI FspAI HincII HindIII HpaI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PsiI PspXI PvuI
SalI ScaI SfiI SgfI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmnI