Restriction map for
pF1KA1090
(Linear) (Six-base) MAPSORT of: KIAA1090.seq Check: 3868 from: 1 to: 4095
KIAA1090.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2125 3120 4095
Size: 2125 995 975
Fragments arranged by size:
2125 995 975
AclI AA'CG_TT
Cuts at: 0 662 4095
Size: 662 3433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1418 1562 1637 1808 1810 1967 2905
Size: 569 849 144 75 171 2 157 938
Cuts at: 2905 2915 3921 4095
Size: 10 1006 174
Fragments arranged by size:
1006 938 849 569 174 171 157 144
75 10 2
AfeI AGC'GCT
Cuts at: 0 2185 4095
Size: 2185 1910
AflIII A'CryG_T
Cuts at: 0 3857 4095
Size: 3857 238
AhdI GACnn_n'nnGTC
Cuts at: 0 3957 4095
Size: 3957 138
AleI CACnn'nnGTG
Cuts at: 0 2288 3478 4095
Size: 2288 1190 617
Fragments arranged by size:
2288 1190 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1445 2053 2421 2709 4095
Size: 1445 608 368 288 1386
Fragments arranged by size:
1445 1386 608 368 288
AlwNI CAG_nnn'CTG
Cuts at: 0 1285 4095
Size: 1285 2810
ApaI G_GGCC'C
Cuts at: 0 960 4095
Size: 960 3135
ApaLI G'TGCA_C
Cuts at: 0 773 4095
Size: 773 3322
ApoI r'AATT_y
Cuts at: 0 4 2209 2749 3259 4095
Size: 4 2205 540 510 836
Fragments arranged by size:
2205 836 540 510 4
AvaI C'yCGr_G
Cuts at: 0 251 1893 2933 3604 4095
Size: 251 1642 1040 671 491
Fragments arranged by size:
1642 1040 671 491 251
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 766 799 1934 1967 4095
Size: 766 33 1135 33 2128
Fragments arranged by size:
2128 1135 766 33 33
BanI G'GyrC_C
Cuts at: 0 69 900 4095
Size: 69 831 3195
Fragments arranged by size:
3195 831 69
BanII G_rGCy'C
Cuts at: 0 960 1907 4095
Size: 960 947 2188
Fragments arranged by size:
2188 960 947
BbsI GAAGACnn'nnnn_
Cuts at: 0 106 1333 1377 1553 1657 2862 4095
Size: 106 1227 44 176 104 1205 1233
Fragments arranged by size:
1233 1227 1205 176 106 104 44
BbvCI CC'TCA_GC
Cuts at: 0 142 1622 4095
Size: 142 1480 2473
Fragments arranged by size:
2473 1480 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1648 1682 1718 1752 4095
Size: 1648 34 36 34 2343
Fragments arranged by size:
2343 1648 36 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1586 4095
Size: 1586 2509
BfrBI ATG'CAT
Cuts at: 0 1777 4095
Size: 1777 2318
BglI GCCn_nnn'nGGC
Cuts at: 0 898 1084 1525 1726 4095
Size: 898 186 441 201 2369
Fragments arranged by size:
2369 898 441 201 186
BglII A'GATC_T
Cuts at: 0 240 572 3011 4095
Size: 240 332 2439 1084
Fragments arranged by size:
2439 1084 332 240
BlpI GC'TnA_GC
Cuts at: 0 1418 4095
Size: 1418 2677
Bme1580I G_kGCm'C
Cuts at: 0 777 960 4095
Size: 777 183 3135
Fragments arranged by size:
3135 777 183
BmgBI CAC'GTC
Cuts at: 0 2484 3858 4095
Size: 2484 1374 237
Fragments arranged by size:
2484 1374 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 954 1368 4095
Size: 954 414 2727
Fragments arranged by size:
2727 954 414
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1283 1749 2148 4095
Size: 1283 466 399 1947
Fragments arranged by size:
1947 1283 466 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 347 556 1255 4095
Size: 144 203 209 699 2840
Fragments arranged by size:
2840 699 209 203 144
Bpu10I CC'TnA_GC
Cuts at: 0 142 1459 1622 4095
Size: 142 1317 163 2473
Fragments arranged by size:
2473 1317 163 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1055 1726 3436 4095
Size: 561 494 671 1710 659
Fragments arranged by size:
1710 671 659 561 494
BsaI GGTCTCn'nnnn_
Cuts at: 0 1222 1313 1887 3184 3439 4095
Size: 1222 91 574 1297 255 656
Fragments arranged by size:
1297 1222 656 574 255 91
BsaAI yAC'GTr
Cuts at: 0 789 4095
Size: 789 3306
BsaBI GATnn'nnATC
Cuts at: 0 3068 4095
Size: 3068 1027
BsaHI Gr'CG_yC
Cuts at: 0 206 806 4095
Size: 206 600 3289
Fragments arranged by size:
3289 600 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1990 2020 3248 3278 4095
Size: 1990 30 1228 30 817
Fragments arranged by size:
1990 1228 817 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 1027 1256 1301 1313 1456 1725 1994
Size: 525 502 229 45 12 143 269 269
Cuts at: 1994 3310 3313 4095
Size: 1316 3 782
Fragments arranged by size:
1316 782 525 502 269 269 229 143
45 12 3
BseYI C'CCAG_C
Cuts at: 0 152 2547 2620 4095
Size: 152 2395 73 1475
Fragments arranged by size:
2395 1475 152 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1949 2421 2617 3759 3927 4095
Size: 1949 472 196 1142 168 168
Fragments arranged by size:
1949 1142 472 196 168 168
BsiHKAI G_wGCw'C
Cuts at: 0 777 1424 1500 2512 3920 4095
Size: 777 647 76 1012 1408 175
Fragments arranged by size:
1408 1012 777 647 175 76
BsmI GAATG_Cn'
Cuts at: 0 1181 4095
Size: 1181 2914
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1892 4095
Size: 1892 2203
Bsp1286I G_dGCh'C
Cuts at: 0 777 960 1424 1500 1907 2512 3920 4095
Size: 777 183 464 76 407 605 1408 175
Fragments arranged by size:
1408 777 605 464 407 183 175 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2125 3120 4095
Size: 2125 995 975
Fragments arranged by size:
2125 995 975
BsrBI CCG'CTC
Cuts at: 0 1017 4095
Size: 1017 3078
BsrDI GCAATG_nn'
Cuts at: 0 1091 2613 3561 4095
Size: 1091 1522 948 534
Fragments arranged by size:
1522 1091 948 534
BsrFI r'CCGG_y
Cuts at: 0 2682 3572 4095
Size: 2682 890 523
Fragments arranged by size:
2682 890 523
BssSI C'ACGA_G
Cuts at: 0 2737 3328 3476 4095
Size: 2737 591 148 619
Fragments arranged by size:
2737 619 591 148
BstAPI GCAn_nnn'nTGC
Cuts at: 0 283 2435 2612 3753 3773 4095
Size: 283 2152 177 1141 20 322
Fragments arranged by size:
2152 1141 322 283 177 20
BstEII G'GTnAC_C
Cuts at: 0 694 4095
Size: 694 3401
BstYI r'GATC_y
Cuts at: 0 240 572 726 3011 4095
Size: 240 332 154 2285 1084
Fragments arranged by size:
2285 1084 332 240 154
Bsu36I CC'TnA_GG
Cuts at: 0 1128 2133 4095
Size: 1128 1005 1962
Fragments arranged by size:
1962 1128 1005
BtgI C'CryG_G
Cuts at: 0 1721 1811 3140 4095
Size: 1721 90 1329 955
Fragments arranged by size:
1721 1329 955 90
BtsI GCAGTG_nn'
Cuts at: 0 93 2438 2450 2881 3600 4095
Size: 93 2345 12 431 719 495
Fragments arranged by size:
2345 719 495 431 93 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3576 3611 4095
Size: 3576 35 484
Fragments arranged by size:
3576 484 35
EaeI y'GGCC_r
Cuts at: 0 198 1697 2680 4095
Size: 198 1499 983 1415
Fragments arranged by size:
1499 1415 983 198
EarI CTCTTCn'nnn_
Cuts at: 0 502 627 829 1169 1186 1252 1290 1445
Size: 502 125 202 340 17 66 38 155
Cuts at: 1445 1666 2859 3697 4045 4095
Size: 221 1193 838 348 50
Fragments arranged by size:
1193 838 502 348 340 221 202 155
125 66 50 38 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1215 4077 4089 4095
Size: 1215 2862 12 6
Fragments arranged by size:
2862 1215 12 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 347 556 569 1255 1418 1562 1637
Size: 144 203 209 13 686 163 144 75
Cuts at: 1637 1808 1810 1967 2905 2915 3921 4095
Size: 171 2 157 938 10 1006 174
Fragments arranged by size:
1006 938 686 209 203 174 171 163
157 144 144 75 13 10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 4095
Size: 172 3923
EcoO109I rG'GnC_Cy
Cuts at: 0 543 956 1599 2050 3225 4095
Size: 543 413 643 451 1175 870
Fragments arranged by size:
1175 870 643 543 451 413
EcoRI G'AATT_C
Cuts at: 0 2749 4095
Size: 2749 1346
EcoRV GAT'ATC
Cuts at: 0 2646 4095
Size: 2646 1449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 577 1050 2652 3837 4095
Size: 429 148 473 1602 1185 258
Fragments arranged by size:
1602 1185 473 429 258 148
HaeII r_GCGC'y
Cuts at: 0 2187 3180 4095
Size: 2187 993 915
Fragments arranged by size:
2187 993 915
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 1354 1386 1667 1699 1891 1923
Size: 564 32 758 32 281 32 192 32
Cuts at: 1923 3943 3975 4095
Size: 2020 32 120
Fragments arranged by size:
2020 758 564 281 192 120 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2487 3267 4095
Size: 2487 780 828
Fragments arranged by size:
2487 828 780
HindIII A'AGCT_T
Cuts at: 0 2917 4095
Size: 2917 1178
HpaI GTT'AAC
Cuts at: 0 3267 4095
Size: 3267 828
MfeI C'AATT_G
Cuts at: 0 3588 4095
Size: 3588 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2333 2447 4095
Size: 2333 114 1648
Fragments arranged by size:
2333 1648 114
MslI CAynn'nnrTG
Cuts at: 0 58 1006 1186 1403 1427 2288 2581 2653
Size: 58 948 180 217 24 861 293 72
Cuts at: 2653 2983 3478 3851 4095
Size: 330 495 373 244
Fragments arranged by size:
948 861 495 373 330 293 244 217
180 72 58 24
MspA1I CmG'CkG
Cuts at: 0 2013 2273 2288 2360 2381 2551 2597 3022
Size: 2013 260 15 72 21 170 46 425
Cuts at: 3022 3142 3428 3731 3750 3941 4095
Size: 120 286 303 19 191 154
Fragments arranged by size:
2013 425 303 286 260 191 170 154
120 72 46 21 19 15
NaeI GCC'GGC
Cuts at: 0 2684 4095
Size: 2684 1411
NcoI C'CATG_G
Cuts at: 0 1721 4095
Size: 1721 2374
NgoMIV G'CCGG_C
Cuts at: 0 2682 4095
Size: 2682 1413
NsiI A_TGCA'T
Cuts at: 0 1779 4095
Size: 1779 2316
NspI r_CATG'y
Cuts at: 0 1005 1781 3357 4095
Size: 1005 776 1576 738
Fragments arranged by size:
1576 1005 776 738
PflMI CCAn_nnn'nTGG
Cuts at: 0 628 1380 4095
Size: 628 752 2715
Fragments arranged by size:
2715 752 628
PfoI T'CCnGG_A
Cuts at: 0 2092 2639 4095
Size: 2092 547 1456
Fragments arranged by size:
2092 1456 547
PpuMI rG'GwC_Cy
Cuts at: 0 1599 2050 4095
Size: 1599 451 2045
Fragments arranged by size:
2045 1599 451
PshAI GACnn'nnGTC
Cuts at: 0 1333 4095
Size: 1333 2762
PspOMI G'GGCC_C
Cuts at: 0 956 4095
Size: 956 3139
PstI C_TGCA'G
Cuts at: 0 173 726 1101 1227 2122 2617 3513 4095
Size: 173 553 375 126 895 495 896 582
Fragments arranged by size:
896 895 582 553 495 375 173 126
PvuII CAG'CTG
Cuts at: 0 2013 2288 2597 3428 3750 3941 4095
Size: 2013 275 309 831 322 191 154
Fragments arranged by size:
2013 831 322 309 275 191 154
SacII CC_GC'GG
Cuts at: 0 3143 4095
Size: 3143 952
SapI GCTCTTCn'nnn_
Cuts at: 0 829 1445 4095
Size: 829 616 2650
Fragments arranged by size:
2650 829 616
SbfI CC_TGCA'GG
Cuts at: 0 173 3513 4095
Size: 173 3340 582
Fragments arranged by size:
3340 582 173
ScaI AGT'ACT
Cuts at: 0 1263 4095
Size: 1263 2832
SfcI C'TryA_G
Cuts at: 0 169 722 1097 1223 1604 2118 2613 2670
Size: 169 553 375 126 381 514 495 57
Cuts at: 2670 3362 3509 4095
Size: 692 147 586
Fragments arranged by size:
692 586 553 514 495 381 375 169
147 126 57
SmaI CCC'GGG
Cuts at: 0 253 1895 4095
Size: 253 1642 2200
Fragments arranged by size:
2200 1642 253
SmlI C'TyrA_G
Cuts at: 0 576 1070 1741 3451 4095
Size: 576 494 671 1710 644
Fragments arranged by size:
1710 671 644 576 494
SspI AAT'ATT
Cuts at: 0 2721 4095
Size: 2721 1374
StuI AGG'CCT
Cuts at: 0 3784 4095
Size: 3784 311
StyI C'CwwG_G
Cuts at: 0 700 1079 1721 2082 2323 3779 3807 3841
Size: 700 379 642 361 241 1456 28 34
Cuts at: 3841 4095
Size: 254
Fragments arranged by size:
1456 700 642 379 361 254 241 34
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 918 1439 1647 2643 3358 3984 4095
Size: 918 521 208 996 715 626 111
Fragments arranged by size:
996 918 715 626 521 208 111
TatI w'GTAC_w
Cuts at: 0 1261 4095
Size: 1261 2834
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 1990 2022 2396 2428 4095
Size: 62 32 1896 32 374 32 1667
Fragments arranged by size:
1896 1667 374 62 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 818 1518 4095
Size: 818 700 2577
Fragments arranged by size:
2577 818 700
XmaI C'CCGG_G
Cuts at: 0 251 1893 4095
Size: 251 1642 2202
Fragments arranged by size:
2202 1642 251
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuII SacII SapI SbfI
ScaI SfcI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AloI AscI AseI
AsiSI AvrII BamHI BbeI BclI BmtI BsaWI BsiEI
BsiWI BspEI BspHI BsrGI BssHII BstBI BstXI BstZ17I
BtgZI ClaI DraI DraIII DrdI EagI EcoICRI FseI
FspI FspAI KasI KpnI MluI MscI NarI NdeI
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmnI ZraI