Restriction map for
pF1KA1091
(Linear) (Six-base) MAPSORT of: KIAA1091.seq Check: 5195 from: 1 to: 3861
KIAA1091.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2132 3861
Size: 2132 1729
AatII G_ACGT'C
Cuts at: 0 3838 3861
Size: 3838 23
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1286 1324 2284 2722 3861
Size: 197 1089 38 960 438 1139
Fragments arranged by size:
1139 1089 960 438 197 38
AfeI AGC'GCT
Cuts at: 0 568 2749 3861
Size: 568 2181 1112
Fragments arranged by size:
2181 1112 568
AflII C'TTAA_G
Cuts at: 0 1387 3861
Size: 1387 2474
AflIII A'CryG_T
Cuts at: 0 3737 3861
Size: 3737 124
AgeI A'CCGG_T
Cuts at: 0 1109 3861
Size: 1109 2752
AhdI GACnn_n'nnGTC
Cuts at: 0 102 3861
Size: 102 3759
AleI CACnn'nnGTG
Cuts at: 0 550 2356 3861
Size: 550 1806 1505
Fragments arranged by size:
1806 1505 550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 3861
Size: 463 3398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3434 3466 3861
Size: 3434 32 395
Fragments arranged by size:
3434 395 32
AlwNI CAG_nnn'CTG
Cuts at: 0 960 3660 3861
Size: 960 2700 201
Fragments arranged by size:
2700 960 201
ApoI r'AATT_y
Cuts at: 0 186 1256 3861
Size: 186 1070 2605
Fragments arranged by size:
2605 1070 186
AvaI C'yCGr_G
Cuts at: 0 877 1817 3861
Size: 877 940 2044
Fragments arranged by size:
2044 940 877
BamHI G'GATC_C
Cuts at: 0 3176 3861
Size: 3176 685
BanI G'GyrC_C
Cuts at: 0 30 148 750 3861
Size: 30 118 602 3111
Fragments arranged by size:
3111 602 118 30
BanII G_rGCy'C
Cuts at: 0 28 344 1700 2729 2905 3585 3861
Size: 28 316 1356 1029 176 680 276
Fragments arranged by size:
1356 1029 680 316 276 176 28
BbeI G_GCGC'C
Cuts at: 0 34 3861
Size: 34 3827
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 1475 2835 3483 3861
Size: 548 927 1360 648 378
Fragments arranged by size:
1360 927 648 548 378
BbvCI CC'TCA_GC
Cuts at: 0 698 3861
Size: 698 3163
BclI T'GATC_A
Cuts at: 0 3758 3861
Size: 3758 103
BglI GCCn_nnn'nGGC
Cuts at: 0 306 3712 3861
Size: 306 3406 149
Fragments arranged by size:
3406 306 149
BglII A'GATC_T
Cuts at: 0 434 3861
Size: 434 3427
Bme1580I G_kGCm'C
Cuts at: 0 915 3861
Size: 915 2946
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 1445 2048 2076 3251 3323 3861
Size: 29 1416 603 28 1175 72 538
Fragments arranged by size:
1416 1175 603 538 72 29 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 1311 1347 3703 3861
Size: 396 915 36 2356 158
Fragments arranged by size:
2356 915 396 158 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 940 1141 1747 2430 3190 3850 3861
Size: 109 831 201 606 683 760 660 11
Fragments arranged by size:
831 760 683 660 606 201 109 11
Bpu10I CC'TnA_GC
Cuts at: 0 698 1694 2012 3861
Size: 698 996 318 1849
Fragments arranged by size:
1849 996 698 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1115 1701 3861
Size: 739 376 586 2160
Fragments arranged by size:
2160 739 586 376
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 509 810 1723 3861
Size: 73 436 301 913 2138
Fragments arranged by size:
2138 913 436 301 73
BsaBI GATnn'nnATC
Cuts at: 0 3465 3861
Size: 3465 396
BsaHI Gr'CG_yC
Cuts at: 0 31 3835 3861
Size: 31 3804 26
Fragments arranged by size:
3804 31 26
BsaWI w'CCGG_w
Cuts at: 0 1109 3266 3861
Size: 1109 2157 595
Fragments arranged by size:
2157 1109 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 877 907 1844 1874 3861
Size: 877 30 937 30 1987
Fragments arranged by size:
1987 937 877 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2701 3861
Size: 2701 1160
BseYI C'CCAG_C
Cuts at: 0 1035 1474 1612 2895 3203 3861
Size: 1035 439 138 1283 308 658
Fragments arranged by size:
1283 1035 658 439 308 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3716 3861
Size: 3716 145
BsiHKAI G_wGCw'C
Cuts at: 0 2729 3861
Size: 2729 1132
BsmI GAATG_Cn'
Cuts at: 0 293 313 1089 1371 2076 2275 3861
Size: 293 20 776 282 705 199 1586
Fragments arranged by size:
1586 776 705 293 282 199 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 612 970 3826 3861
Size: 612 358 2856 35
Fragments arranged by size:
2856 612 358 35
Bsp1286I G_dGCh'C
Cuts at: 0 28 344 915 1700 2729 2905 3585 3861
Size: 28 316 571 785 1029 176 680 276
Fragments arranged by size:
1029 785 680 571 316 276 176 28
BspEI T'CCGG_A
Cuts at: 0 3266 3861
Size: 3266 595
BspHI T'CATG_A
Cuts at: 0 644 1773 3861
Size: 644 1129 2088
Fragments arranged by size:
2088 1129 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1712 2132 3861
Size: 1712 420 1729
Fragments arranged by size:
1729 1712 420
BsrBI CCG'CTC
Cuts at: 0 756 3174 3861
Size: 756 2418 687
Fragments arranged by size:
2418 756 687
BsrDI GCAATG_nn'
Cuts at: 0 452 1278 1352 3805 3861
Size: 452 826 74 2453 56
Fragments arranged by size:
2453 826 452 74 56
BsrFI r'CCGG_y
Cuts at: 0 770 1109 2480 3134 3861
Size: 770 339 1371 654 727
Fragments arranged by size:
1371 770 727 654 339
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3807 3861
Size: 3807 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1101 1212 2175 3072 3861
Size: 1101 111 963 897 789
Fragments arranged by size:
1101 963 897 789 111
BstYI r'GATC_y
Cuts at: 0 434 1488 1544 2405 2582 2927 3176 3320
Size: 434 1054 56 861 177 345 249 144
Cuts at: 3320 3861
Size: 541
Fragments arranged by size:
1054 861 541 434 345 249 177 144
56
Bsu36I CC'TnA_GG
Cuts at: 0 2517 2578 3209 3560 3861
Size: 2517 61 631 351 301
Fragments arranged by size:
2517 631 351 301 61
BtgI C'CryG_G
Cuts at: 0 2672 2923 3861
Size: 2672 251 938
Fragments arranged by size:
2672 938 251
BtsI GCAGTG_nn'
Cuts at: 0 447 709 1290 2912 3605 3728 3861
Size: 447 262 581 1622 693 123 133
Fragments arranged by size:
1622 693 581 447 262 133 123
DraI TTT'AAA
Cuts at: 0 3465 3861
Size: 3465 396
DrdI GACnn_nn'nnGTC
Cuts at: 0 55 853 3861
Size: 55 798 3008
Fragments arranged by size:
3008 798 55
EaeI y'GGCC_r
Cuts at: 0 768 3039 3132 3861
Size: 768 2271 93 729
Fragments arranged by size:
2271 768 729 93
EarI CTCTTCn'nnn_
Cuts at: 0 410 1300 2357 3484 3861
Size: 410 890 1057 1127 377
Fragments arranged by size:
1127 1057 890 410 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 32 988 2106 2179 3861
Size: 32 956 1118 73 1682
Fragments arranged by size:
1682 1118 956 73 32
EcoICRI GAG'CTC
Cuts at: 0 2727 3861
Size: 2727 1134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 197 940 1141 1286 1324 1747 2284
Size: 109 88 743 201 145 38 423 537
Cuts at: 2284 2430 2722 3190 3850 3861
Size: 146 292 468 660 11
Fragments arranged by size:
743 660 537 468 423 292 201 146
145 109 88 38 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2263 2593 3844 3861
Size: 2263 330 1251 17
Fragments arranged by size:
2263 1251 330 17
EcoO109I rG'GnC_Cy
Cuts at: 0 228 2256 3861
Size: 228 2028 1605
Fragments arranged by size:
2028 1605 228
EcoRI G'AATT_C
Cuts at: 0 1256 3861
Size: 1256 2605
EcoRV GAT'ATC
Cuts at: 0 241 3861
Size: 241 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1291 3861
Size: 1291 2570
HaeII r_GCGC'y
Cuts at: 0 34 570 2751 3861
Size: 34 536 2181 1110
Fragments arranged by size:
2181 1110 536 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 972 1004 1096 1128 1612 1644
Size: 88 32 852 32 92 32 484 32
Cuts at: 1644 1657 1689 1842 1874 2397 2429 2545 2577
Size: 13 32 153 32 523 32 116 32
Cuts at: 2577 3861
Size: 1284
Fragments arranged by size:
1284 852 523 484 153 116 92 88
32 32 32 32 32 32 32 32
13
HincII GTy'rAC
Cuts at: 0 1824 3861
Size: 1824 2037
HindIII A'AGCT_T
Cuts at: 0 3019 3861
Size: 3019 842
KasI G'GCGC_C
Cuts at: 0 30 3861
Size: 30 3831
MfeI C'AATT_G
Cuts at: 0 1913 2238 3861
Size: 1913 325 1623
Fragments arranged by size:
1913 1623 325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1436 1632 2231 3861
Size: 637 799 196 599 1630
Fragments arranged by size:
1630 799 637 599 196
MscI TGG'CCA
Cuts at: 0 3041 3861
Size: 3041 820
MslI CAynn'nnrTG
Cuts at: 0 550 634 1019 1094 1283 1576 1778 2356
Size: 550 84 385 75 189 293 202 578
Cuts at: 2356 3740 3861
Size: 1384 121
Fragments arranged by size:
1384 578 550 385 293 202 189 121
84 75
MspA1I CmG'CkG
Cuts at: 0 3243 3861
Size: 3243 618
NarI GG'CG_CC
Cuts at: 0 31 3861
Size: 31 3830
NcoI C'CATG_G
Cuts at: 0 2672 2923 3861
Size: 2672 251 938
Fragments arranged by size:
2672 938 251
NspI r_CATG'y
Cuts at: 0 468 496 656 1018 3741 3861
Size: 468 28 160 362 2723 120
Fragments arranged by size:
2723 468 362 160 120 28
PasI CC'CwG_GG
Cuts at: 0 335 3861
Size: 335 3526
PciI A'CATG_T
Cuts at: 0 3737 3861
Size: 3737 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 766 2657 2924 3012 3861
Size: 766 1891 267 88 849
Fragments arranged by size:
1891 849 766 267 88
PfoI T'CCnGG_A
Cuts at: 0 1223 1547 1724 2159 2396 3323 3506 3861
Size: 1223 324 177 435 237 927 183 355
Fragments arranged by size:
1223 927 435 355 324 237 183 177
PpuMI rG'GwC_Cy
Cuts at: 0 228 3861
Size: 228 3633
PstI C_TGCA'G
Cuts at: 0 442 566 671 2021 3248 3803 3861
Size: 442 124 105 1350 1227 555 58
Fragments arranged by size:
1350 1227 555 442 124 105 58
SacI G_AGCT'C
Cuts at: 0 2729 3861
Size: 2729 1132
SapI GCTCTTCn'nnn_
Cuts at: 0 3484 3861
Size: 3484 377
SfcI C'TryA_G
Cuts at: 0 438 562 667 2017 3244 3799 3861
Size: 438 124 105 1350 1227 555 62
Fragments arranged by size:
1350 1227 555 438 124 105 62
SfoI GGC'GCC
Cuts at: 0 32 3861
Size: 32 3829
SmlI C'TyrA_G
Cuts at: 0 718 1130 1387 1716 3861
Size: 718 412 257 329 2145
Fragments arranged by size:
2145 718 412 329 257
StyI C'CwwG_G
Cuts at: 0 1421 2672 2923 3437 3861
Size: 1421 1251 251 514 424
Fragments arranged by size:
1421 1251 514 424 251
SwaI ATTT'AAAT
Cuts at: 0 3465 3861
Size: 3465 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 1970 2649 2938 3303 3861
Size: 648 1322 679 289 365 558
Fragments arranged by size:
1322 679 648 558 365 289
TatI w'GTAC_w
Cuts at: 0 119 1797 2028 2153 3861
Size: 119 1678 231 125 1708
Fragments arranged by size:
1708 1678 231 125 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1844 1876 2411 2443 3861
Size: 1844 32 535 32 1418
Fragments arranged by size:
1844 1418 535 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2292 3049 3072 3861
Size: 2292 757 23 789
Fragments arranged by size:
2292 789 757 23
XmnI GAAnn'nnTTC
Cuts at: 0 3364 3861
Size: 3364 497
ZraI GAC'GTC
Cuts at: 0 3836 3861
Size: 3836 25
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BclI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NspI PasI PciI
PflMI PfoI PpuMI PstI SacI SapI SfcI SfoI
SmlI StyI SwaI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BfrBI BlpI BmgBI BmtI
BsaAI BsiEI BsiWI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraIII EagI FseI FspI
FspAI HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI Tth111I
XbaI XhoI XmaI