Restriction map for
pF1KSDA1092
(Linear) (Six-base) MAPSORT of: KIAA1092.seq Check: 7291 from: 1 to: 3381
KIAA1092.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
Acc65I G'GTAC_C
Cuts at: 0 740 967 3381
Size: 740 227 2414
Fragments arranged by size:
2414 740 227
AclI AA'CG_TT
Cuts at: 0 2007 3381
Size: 2007 1374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1514 2202 3010 3381
Size: 659 855 688 808 371
Fragments arranged by size:
855 808 688 659 371
AflII C'TTAA_G
Cuts at: 0 928 3381
Size: 928 2453
AlwNI CAG_nnn'CTG
Cuts at: 0 1871 3058 3381
Size: 1871 1187 323
Fragments arranged by size:
1871 1187 323
ApaI G_GGCC'C
Cuts at: 0 39 58 2909 3381
Size: 39 19 2851 472
Fragments arranged by size:
2851 472 39 19
ApaLI G'TGCA_C
Cuts at: 0 2404 3381
Size: 2404 977
ApoI r'AATT_y
Cuts at: 0 470 988 1028 1066 1894 2446 2518 3100
Size: 470 518 40 38 828 552 72 582
Cuts at: 3100 3381
Size: 281
Fragments arranged by size:
828 582 552 518 470 281 72 40
38
AseI AT'TA_AT
Cuts at: 0 419 3381
Size: 419 2962
AvaI C'yCGr_G
Cuts at: 0 51 203 278 3381
Size: 51 152 75 3103
Fragments arranged by size:
3103 152 75 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 724 757 959 992 3381
Size: 724 33 202 33 2389
Fragments arranged by size:
2389 724 202 33 33
BanI G'GyrC_C
Cuts at: 0 22 67 76 740 967 2317 3127 3301
Size: 22 45 9 664 227 1350 810 174
Cuts at: 3301 3381
Size: 80
Fragments arranged by size:
1350 810 664 227 174 80 45 22
9
BanII G_rGCy'C
Cuts at: 0 39 58 1386 2909 3381
Size: 39 19 1328 1523 472
Fragments arranged by size:
1523 1328 472 39 19
BbeI G_GCGC'C
Cuts at: 0 26 71 80 3381
Size: 26 45 9 3301
Fragments arranged by size:
3301 45 26 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 1547 3381
Size: 1547 1834
BfrBI ATG'CAT
Cuts at: 0 1519 2716 3381
Size: 1519 1197 665
Fragments arranged by size:
1519 1197 665
BglI GCCn_nnn'nGGC
Cuts at: 0 20 34 3381
Size: 20 14 3347
Fragments arranged by size:
3347 20 14
BglII A'GATC_T
Cuts at: 0 2793 3213 3381
Size: 2793 420 168
Fragments arranged by size:
2793 420 168
Bme1580I G_kGCm'C
Cuts at: 0 39 58 2408 2909 3381
Size: 39 19 2350 501 472
Fragments arranged by size:
2350 501 472 39 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 1935 2024 3381
Size: 1935 89 1357
Fragments arranged by size:
1935 1357 89
BmtI G_CTAG'C
Cuts at: 0 212 3381
Size: 212 3169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 1642 2226 3381
Size: 139 1503 584 1155
Fragments arranged by size:
1503 1155 584 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2615 3381
Size: 2615 766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 2051 3016 3381
Size: 1120 931 965 365
Fragments arranged by size:
1120 965 931 365
BsaI GGTCTCn'nnnn_
Cuts at: 0 2214 2256 3381
Size: 2214 42 1125
Fragments arranged by size:
2214 1125 42
BsaAI yAC'GTr
Cuts at: 0 2631 3381
Size: 2631 750
BsaHI Gr'CG_yC
Cuts at: 0 23 68 77 3381
Size: 23 45 9 3304
Fragments arranged by size:
3304 45 23 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2218 2666 2669 2841 3381
Size: 2218 448 3 172 540
Fragments arranged by size:
2218 540 448 172 3
BseYI C'CCAG_C
Cuts at: 0 253 2768 3381
Size: 253 2515 613
Fragments arranged by size:
2515 613 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2402 3381
Size: 2402 979
BsiEI CG_ry'CG
Cuts at: 0 15 263 3381
Size: 15 248 3118
Fragments arranged by size:
3118 248 15
BsiHKAI G_wGCw'C
Cuts at: 0 1386 2408 3381
Size: 1386 1022 973
Fragments arranged by size:
1386 1022 973
BsmI GAATG_Cn'
Cuts at: 0 1519 2769 2820 3381
Size: 1519 1250 51 561
Fragments arranged by size:
1519 1250 561 51
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2411 3381
Size: 2411 970
Bsp1286I G_dGCh'C
Cuts at: 0 39 58 1386 2408 2909 3381
Size: 39 19 1328 1022 501 472
Fragments arranged by size:
1328 1022 501 472 39 19
BspHI T'CATG_A
Cuts at: 0 1005 2195 3381
Size: 1005 1190 1186
Fragments arranged by size:
1190 1186 1005
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2468 3381
Size: 2468 913
BsrDI GCAATG_nn'
Cuts at: 0 621 886 3381
Size: 621 265 2495
Fragments arranged by size:
2495 621 265
BsrFI r'CCGG_y
Cuts at: 0 28 3381
Size: 28 3353
BssHII G'CGCG_C
Cuts at: 0 139 3381
Size: 139 3242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 712 1525 3381
Size: 712 813 1856
Fragments arranged by size:
1856 813 712
BstYI r'GATC_y
Cuts at: 0 2337 2793 3213 3381
Size: 2337 456 420 168
Fragments arranged by size:
2337 456 420 168
Bsu36I CC'TnA_GG
Cuts at: 0 514 3381
Size: 514 2867
BtgI C'CryG_G
Cuts at: 0 129 228 2361 2474 2744 3095 3381
Size: 129 99 2133 113 270 351 286
Fragments arranged by size:
2133 351 286 270 129 113 99
BtsI GCAGTG_nn'
Cuts at: 0 408 1497 2878 3173 3381
Size: 408 1089 1381 295 208
Fragments arranged by size:
1381 1089 408 295 208
DraI TTT'AAA
Cuts at: 0 908 3381
Size: 908 2473
EaeI y'GGCC_r
Cuts at: 0 12 126 2185 2471 2526 2858 3381
Size: 12 114 2059 286 55 332 523
Fragments arranged by size:
2059 523 332 286 114 55 12
EagI C'GGCC_G
Cuts at: 0 12 3381
Size: 12 3369
EarI CTCTTCn'nnn_
Cuts at: 0 337 2643 3381
Size: 337 2306 738
Fragments arranged by size:
2306 738 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3381
Size: 19 3362
EcoICRI GAG'CTC
Cuts at: 0 1384 3381
Size: 1384 1997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1514 2202 2615 3010 3381
Size: 659 855 688 413 395 371
Fragments arranged by size:
855 688 659 413 395 371
EcoO109I rG'GnC_Cy
Cuts at: 0 35 36 223 1083 1354 2601 2676 2905
Size: 35 1 187 860 271 1247 75 229
Cuts at: 2905 3381
Size: 476
Fragments arranged by size:
1247 860 476 271 229 187 75 35
1
EcoRI G'AATT_C
Cuts at: 0 1066 2518 3100 3381
Size: 1066 1452 582 281
Fragments arranged by size:
1452 1066 582 281
EcoRV GAT'ATC
Cuts at: 0 635 3381
Size: 635 2746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1087 3232 3381
Size: 1087 2145 149
Fragments arranged by size:
2145 1087 149
HaeII r_GCGC'y
Cuts at: 0 26 71 80 2225 3381
Size: 26 45 9 2145 1156
Fragments arranged by size:
2145 1156 45 26 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 619 651 3381
Size: 550 32 37 32 2730
Fragments arranged by size:
2730 550 37 32 32
HindIII A'AGCT_T
Cuts at: 0 2439 3381
Size: 2439 942
KasI G'GCGC_C
Cuts at: 0 22 67 76 3381
Size: 22 45 9 3305
Fragments arranged by size:
3305 45 22 9
KpnI G_GTAC'C
Cuts at: 0 744 971 3381
Size: 744 227 2410
Fragments arranged by size:
2410 744 227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 485 1385 3381
Size: 188 297 900 1996
Fragments arranged by size:
1996 900 297 188
MscI TGG'CCA
Cuts at: 0 2473 2860 3381
Size: 2473 387 521
Fragments arranged by size:
2473 521 387
MslI CAynn'nnrTG
Cuts at: 0 1127 1160 2411 3381
Size: 1127 33 1251 970
Fragments arranged by size:
1251 1127 970 33
MspA1I CmG'CkG
Cuts at: 0 152 230 3381
Size: 152 78 3151
Fragments arranged by size:
3151 152 78
NaeI GCC'GGC
Cuts at: 0 30 3381
Size: 30 3351
NarI GG'CG_CC
Cuts at: 0 23 68 77 3381
Size: 23 45 9 3304
Fragments arranged by size:
3304 45 23 9
NcoI C'CATG_G
Cuts at: 0 2361 2474 3095 3381
Size: 2361 113 621 286
Fragments arranged by size:
2361 621 286 113
NgoMIV G'CCGG_C
Cuts at: 0 28 3381
Size: 28 3353
NheI G'CTAG_C
Cuts at: 0 208 3381
Size: 208 3173
NsiI A_TGCA'T
Cuts at: 0 1521 2718 3381
Size: 1521 1197 663
Fragments arranged by size:
1521 1197 663
NspI r_CATG'y
Cuts at: 0 381 507 2614 2811 2880 3381
Size: 381 126 2107 197 69 501
Fragments arranged by size:
2107 501 381 197 126 69
PasI CC'CwG_GG
Cuts at: 0 2245 3381
Size: 2245 1136
PmlI CAC'GTG
Cuts at: 0 2631 3381
Size: 2631 750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1330 1362 3381
Size: 916 32 382 32 2019
Fragments arranged by size:
2019 916 382 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 223 1083 1354 2601 3381
Size: 223 860 271 1247 780
Fragments arranged by size:
1247 860 780 271 223
PsiI TTA'TAA
Cuts at: 0 1309 3381
Size: 1309 2072
PspOMI G'GGCC_C
Cuts at: 0 35 54 2905 3381
Size: 35 19 2851 476
Fragments arranged by size:
2851 476 35 19
PstI C_TGCA'G
Cuts at: 0 178 2585 2969 3381
Size: 178 2407 384 412
Fragments arranged by size:
2407 412 384 178
SacI G_AGCT'C
Cuts at: 0 1386 3381
Size: 1386 1995
SacII CC_GC'GG
Cuts at: 0 231 3381
Size: 231 3150
SanDI GG'GwC_CC
Cuts at: 0 223 3381
Size: 223 3158
ScaI AGT'ACT
Cuts at: 0 1924 3381
Size: 1924 1457
SfcI C'TryA_G
Cuts at: 0 174 2581 2965 3381
Size: 174 2407 384 416
Fragments arranged by size:
2407 416 384 174
SfoI GGC'GCC
Cuts at: 0 24 69 78 3381
Size: 24 45 9 3303
Fragments arranged by size:
3303 45 24 9
SgrAI Cr'CCGG_yG
Cuts at: 0 28 3381
Size: 28 3353
SmaI CCC'GGG
Cuts at: 0 53 205 280 3381
Size: 53 152 75 3101
Fragments arranged by size:
3101 152 75 53
SmlI C'TyrA_G
Cuts at: 0 928 1099 2066 3031 3381
Size: 928 171 967 965 350
Fragments arranged by size:
967 965 928 350 171
SphI G_CATG'C
Cuts at: 0 381 3381
Size: 381 3000
SrfI GCCC'GGGC
Cuts at: 0 205 3381
Size: 205 3176
SspI AAT'ATT
Cuts at: 0 2142 3189 3381
Size: 2142 1047 192
Fragments arranged by size:
2142 1047 192
StyI C'CwwG_G
Cuts at: 0 71 849 1079 2361 2474 3095 3131 3381
Size: 71 778 230 1282 113 621 36 250
Fragments arranged by size:
1282 778 621 250 230 113 71 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1656 3381
Size: 1656 1725
TatI w'GTAC_w
Cuts at: 0 1015 1922 2531 3381
Size: 1015 907 609 850
Fragments arranged by size:
1015 907 850 609
Tth111I GACn'n_nGTC
Cuts at: 0 2239 3381
Size: 2239 1142
XbaI T'CTAG_A
Cuts at: 0 466 3381
Size: 466 2915
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2328 3381
Size: 2328 1053
XmaI C'CCGG_G
Cuts at: 0 51 203 278 3381
Size: 51 152 75 3103
Fragments arranged by size:
3103 152 75 51
XmnI GAAnn'nnTTC
Cuts at: 0 1151 2046 3381
Size: 1151 895 1335
Fragments arranged by size:
1335 1151 895
Enzymes that do cut:
Acc65I AclI AcuI AflII AlwNI ApaI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbeI BciVI BfrBI
BglI BglII Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssHII
BstAPI BstYI Bsu36I BtgI BtsI DraI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PmlI PpiI PpuMI PsiI PspOMI PstI SacI
SacII SanDI ScaI SfcI SfoI SgrAI SmaI SmlI
SphI SrfI SspI StyI TaqII TatI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflIII AgeI AhdI AleI
AlfI AloI AscI AsiSI AvrII BamHI BbsI BbvCI
BcgI BclI BlpI BmgBI Bpu10I BsaBI BsaWI BsaXI
BsiWI BspEI BsrBI BsrGI BssSI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraIII DrdI EcoNI FseI
FspI FspAI HincII HpaI MfeI MluI NdeI NotI
NruI PacI PciI PflMI PfoI PmeI PshAI PspXI
PsrI PvuI PvuII RsrII SalI SapI SbfI SexAI
SfiI SgfI SnaBI SpeI StuI SwaI TstI XhoI
ZraI