Restriction map for
pF1KSDA1093
(Linear) (Six-base) MAPSORT of: KIAA1093.seq Check: 1532 from: 1 to: 5166
KIAA1093.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4878 5166
Size: 4878 288
AccI GT'mk_AC
Cuts at: 0 575 1435 5166
Size: 575 860 3731
Fragments arranged by size:
3731 860 575
Acc65I G'GTAC_C
Cuts at: 0 3717 5166
Size: 3717 1449
AclI AA'CG_TT
Cuts at: 0 2549 4328 5166
Size: 2549 1779 838
Fragments arranged by size:
2549 1779 838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 2663 3086 3774 4325 5166
Size: 879 1784 423 688 551 841
Fragments arranged by size:
1784 879 841 688 551 423
AfeI AGC'GCT
Cuts at: 0 425 1965 5001 5166
Size: 425 1540 3036 165
Fragments arranged by size:
3036 1540 425 165
AflII C'TTAA_G
Cuts at: 0 1146 5166
Size: 1146 4020
AflIII A'CryG_T
Cuts at: 0 4778 5166
Size: 4778 388
AleI CACnn'nnGTG
Cuts at: 0 3310 4781 5166
Size: 3310 1471 385
Fragments arranged by size:
3310 1471 385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1367 1399 5166
Size: 1367 32 3767
Fragments arranged by size:
3767 1367 32
AlwNI CAG_nnn'CTG
Cuts at: 0 388 1312 3606 4911 5166
Size: 388 924 2294 1305 255
Fragments arranged by size:
2294 1305 924 388 255
ApaI G_GGCC'C
Cuts at: 0 2752 3446 3695 5051 5166
Size: 2752 694 249 1356 115
Fragments arranged by size:
2752 1356 694 249 115
ApaLI G'TGCA_C
Cuts at: 0 998 3623 5166
Size: 998 2625 1543
Fragments arranged by size:
2625 1543 998
ApoI r'AATT_y
Cuts at: 0 495 1097 1593 2736 3121 3324 3491 4102
Size: 495 602 496 1143 385 203 167 611
Cuts at: 4102 5166
Size: 1064
Fragments arranged by size:
1143 1064 611 602 496 495 385 203
167
AvaI C'yCGr_G
Cuts at: 0 263 510 2759 3151 3950 4981 5166
Size: 263 247 2249 392 799 1031 185
Fragments arranged by size:
2249 1031 799 392 263 247 185
BanI G'GyrC_C
Cuts at: 0 270 532 1914 3717 4288 5166
Size: 270 262 1382 1803 571 878
Fragments arranged by size:
1803 1382 878 571 270 262
BanII G_rGCy'C
Cuts at: 0 511 2048 2752 2862 3446 3695 3743 4982
Size: 511 1537 704 110 584 249 48 1239
Cuts at: 4982 5051 5166
Size: 69 115
Fragments arranged by size:
1537 1239 704 584 511 249 115 110
69 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 2219 5166
Size: 47 2172 2947
Fragments arranged by size:
2947 2172 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 269 303 3282 3316 3592 3626 4653 4687
Size: 269 34 2979 34 276 34 1027 34
Cuts at: 4687 5166
Size: 479
Fragments arranged by size:
2979 1027 479 276 269 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4141 5166
Size: 4141 1025
BglI GCCn_nnn'nGGC
Cuts at: 0 167 5166
Size: 167 4999
BglII A'GATC_T
Cuts at: 0 1678 5166
Size: 1678 3488
BlpI GC'TnA_GC
Cuts at: 0 3475 4856 5166
Size: 3475 1381 310
Fragments arranged by size:
3475 1381 310
Bme1580I G_kGCm'C
Cuts at: 0 119 1002 2752 3446 3627 3695 4720 5051
Size: 119 883 1750 694 181 68 1025 331
Cuts at: 5051 5166
Size: 115
Fragments arranged by size:
1750 1025 883 694 331 181 119 115
68
BmrI ACTGGGnnnn_n'
Cuts at: 0 1265 1406 1553 2133 2609 3329 3354 3441
Size: 1265 141 147 580 476 720 25 87
Cuts at: 3441 3492 4009 4985 5166
Size: 51 517 976 181
Fragments arranged by size:
1265 976 720 580 517 476 181 147
141 87 51 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1194 1262 1625 2525 2592 4082 4425
Size: 410 784 68 363 900 67 1490 343
Cuts at: 4425 4527 4599 4933 5166
Size: 102 72 334 233
Fragments arranged by size:
1490 900 784 410 363 343 334 233
102 72 68 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3379 3560 4677 5166
Size: 3379 181 1117 489
Fragments arranged by size:
3379 1117 489 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 1521 2684 4909 5166
Size: 1521 1163 2225 257
Fragments arranged by size:
2225 1521 1163 257
BsaHI Gr'CG_yC
Cuts at: 0 2205 5166
Size: 2205 2961
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 520 550 1120 1150 1367 1397 4572 4602
Size: 520 30 570 30 217 30 3175 30
Cuts at: 4602 5166
Size: 564
Fragments arranged by size:
3175 570 564 520 217 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 510 2144 2896 2899 2909 3446 5162
Size: 362 148 1634 752 3 10 537 1716
Cuts at: 5162 5166
Size: 4
Fragments arranged by size:
1716 1634 752 537 362 148 10 4
3
BseYI C'CCAG_C
Cuts at: 0 1865 1994 2008 2176 2409 2751 2828 2869
Size: 1865 129 14 168 233 342 77 41
Cuts at: 2869 2873 2951 4182 5166
Size: 4 78 1231 984
Fragments arranged by size:
1865 1231 984 342 233 168 129 78
77 41 14 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 1298 3286 3334 4754 4759 5166
Size: 363 935 1988 48 1420 5 407
Fragments arranged by size:
1988 1420 935 407 363 48 5
BsiEI CG_ry'CG
Cuts at: 0 155 1774 5166
Size: 155 1619 3392
Fragments arranged by size:
3392 1619 155
BsiHKAI G_wGCw'C
Cuts at: 0 688 1002 1219 1853 2048 2862 2986 3627
Size: 688 314 217 634 195 814 124 641
Cuts at: 3627 5166
Size: 1539
Fragments arranged by size:
1539 814 688 641 634 314 217 195
124
BsmI GAATG_Cn'
Cuts at: 0 1184 5166
Size: 1184 3982
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2416 5166
Size: 2416 2750
Bsp1286I G_dGCh'C
Cuts at: 0 119 511 688 1002 1219 1853 2048 2752
Size: 119 392 177 314 217 634 195 704
Cuts at: 2752 2862 2986 3446 3627 3695 3743 4720 4982
Size: 110 124 460 181 68 48 977 262
Cuts at: 4982 5051 5166
Size: 69 115
Fragments arranged by size:
977 704 634 460 392 314 262 217
195 181 177 124 119 115 110 69
68 48
BspHI T'CATG_A
Cuts at: 0 3170 5166
Size: 3170 1996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2780 4047 4878 4956 5166
Size: 2780 1267 831 78 210
Fragments arranged by size:
2780 1267 831 210 78
BsrDI GCAATG_nn'
Cuts at: 0 1659 2367 4089 5166
Size: 1659 708 1722 1077
Fragments arranged by size:
1722 1659 1077 708
BsrFI r'CCGG_y
Cuts at: 0 4918 5166
Size: 4918 248
BstEII G'GTnAC_C
Cuts at: 0 3959 4034 5134 5166
Size: 3959 75 1100 32
Fragments arranged by size:
3959 1100 75 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 929 3216 5166
Size: 216 713 2287 1950
Fragments arranged by size:
2287 1950 713 216
BstYI r'GATC_y
Cuts at: 0 1267 1678 2404 4380 4935 5094 5166
Size: 1267 411 726 1976 555 159 72
Fragments arranged by size:
1976 1267 726 555 411 159 72
Bsu36I CC'TnA_GG
Cuts at: 0 788 1925 2600 5166
Size: 788 1137 675 2566
Fragments arranged by size:
2566 1137 788 675
BtgI C'CryG_G
Cuts at: 0 1163 4120 4940 5166
Size: 1163 2957 820 226
Fragments arranged by size:
2957 1163 820 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 3157 5166
Size: 164 2993 2009
Fragments arranged by size:
2993 2009 164
BtsI GCAGTG_nn'
Cuts at: 0 227 2931 3625 4684 4718 4771 4993 5166
Size: 227 2704 694 1059 34 53 222 173
Fragments arranged by size:
2704 1059 694 227 222 173 53 34
ClaI AT'CG_AT
Cuts at: 0 3183 5166
Size: 3183 1983
DraI TTT'AAA
Cuts at: 0 3855 5166
Size: 3855 1311
DraIII CAC_nnn'GTG
Cuts at: 0 306 1898 4003 4288 5166
Size: 306 1592 2105 285 878
Fragments arranged by size:
2105 1592 878 306 285
DrdI GACnn_nn'nnGTC
Cuts at: 0 2848 4162 5166
Size: 2848 1314 1004
Fragments arranged by size:
2848 1314 1004
EaeI y'GGCC_r
Cuts at: 0 152 931 1764 4005 4754 4920 5166
Size: 152 779 833 2241 749 166 246
Fragments arranged by size:
2241 833 779 749 246 166 152
EagI C'GGCC_G
Cuts at: 0 152 5166
Size: 152 5014
EarI CTCTTCn'nnn_
Cuts at: 0 587 676 1904 2121 2621 2873 5166
Size: 587 89 1228 217 500 252 2293
Fragments arranged by size:
2293 1228 587 500 252 217 89
EcoICRI GAG'CTC
Cuts at: 0 2046 2860 5166
Size: 2046 814 2306
Fragments arranged by size:
2306 2046 814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 879 1194 1262 1625 2525 2592 2663
Size: 410 469 315 68 363 900 67 71
Cuts at: 2663 3086 3774 4082 4325 4425 4527 4599 4933
Size: 423 688 308 243 100 102 72 334
Cuts at: 4933 5166
Size: 233
Fragments arranged by size:
900 688 469 423 410 363 334 315
308 243 233 102 100 72 71 68
67
EcoO109I rG'GnC_Cy
Cuts at: 0 367 1288 1445 2748 3442 3443 4015 4945
Size: 367 921 157 1303 694 1 572 930
Cuts at: 4945 5047 5048 5166
Size: 102 1 118
Fragments arranged by size:
1303 930 921 694 572 367 157 118
102 1 1
EcoRI G'AATT_C
Cuts at: 0 2736 4102 5166
Size: 2736 1366 1064
Fragments arranged by size:
2736 1366 1064
EcoRV GAT'ATC
Cuts at: 0 3168 3981 5166
Size: 3168 813 1185
Fragments arranged by size:
3168 1185 813
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2390 5166
Size: 2390 2776
HaeII r_GCGC'y
Cuts at: 0 248 427 1967 3929 5003 5036 5166
Size: 248 179 1540 1962 1074 33 130
Fragments arranged by size:
1962 1540 1074 248 179 130 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 1294 1326 1918 1950 2797 2829
Size: 610 32 652 32 592 32 847 32
Cuts at: 2829 3006 3038 3165 3197 3411 3443 4135 4167
Size: 177 32 127 32 214 32 692 32
Cuts at: 4167 4348 4380 4576 4608 4683 4715 5166
Size: 181 32 196 32 75 32 451
Fragments arranged by size:
847 692 652 610 592 451 214 196
181 177 127 75 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 4652 5166
Size: 4652 514
KpnI G_GTAC'C
Cuts at: 0 3721 5166
Size: 3721 1445
MfeI C'AATT_G
Cuts at: 0 164 2277 5166
Size: 164 2113 2889
Fragments arranged by size:
2889 2113 164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 836 1245 1686 2144 2632 4131 4436
Size: 548 288 409 441 458 488 1499 305
Cuts at: 4436 5135 5166
Size: 699 31
Fragments arranged by size:
1499 699 548 488 458 441 409 305
288 31
MscI TGG'CCA
Cuts at: 0 933 4007 5166
Size: 933 3074 1159
Fragments arranged by size:
3074 1159 933
MslI CAynn'nnrTG
Cuts at: 0 2782 3310 3920 4781 4783 5166
Size: 2782 528 610 861 2 383
Fragments arranged by size:
2782 861 610 528 383 2
MspA1I CmG'CkG
Cuts at: 0 2570 2579 2873 3606 4458 4894 5015 5166
Size: 2570 9 294 733 852 436 121 151
Fragments arranged by size:
2570 852 733 436 294 151 121 9
NcoI C'CATG_G
Cuts at: 0 1163 4120 5166
Size: 1163 2957 1046
Fragments arranged by size:
2957 1163 1046
NspI r_CATG'y
Cuts at: 0 261 348 3515 4674 4782 5166
Size: 261 87 3167 1159 108 384
Fragments arranged by size:
3167 1159 384 261 108 87
PasI CC'CwG_GG
Cuts at: 0 1844 4713 5166
Size: 1844 2869 453
Fragments arranged by size:
2869 1844 453
PciI A'CATG_T
Cuts at: 0 4778 5166
Size: 4778 388
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 1123 2444 2621 3772 4474 5106 5166
Size: 193 930 1321 177 1151 702 632 60
Fragments arranged by size:
1321 1151 930 702 632 193 177 60
PfoI T'CCnGG_A
Cuts at: 0 1210 1366 1447 2443 4441 5166
Size: 1210 156 81 996 1998 725
Fragments arranged by size:
1998 1210 996 725 156 81
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1367 1399 2098 2130 3831 3863
Size: 658 32 677 32 699 32 1701 32
Cuts at: 3863 5166
Size: 1303
Fragments arranged by size:
1701 1303 699 677 658 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 1445 4015 4945 5166
Size: 1445 2570 930 221
Fragments arranged by size:
2570 1445 930 221
PsiI TTA'TAA
Cuts at: 0 2705 5166
Size: 2705 2461
PspOMI G'GGCC_C
Cuts at: 0 2748 3442 3691 5047 5166
Size: 2748 694 249 1356 119
Fragments arranged by size:
2748 1356 694 249 119
PstI C_TGCA'G
Cuts at: 0 434 3632 4875 5166
Size: 434 3198 1243 291
Fragments arranged by size:
3198 1243 434 291
PvuII CAG'CTG
Cuts at: 0 2570 2873 3606 4894 5166
Size: 2570 303 733 1288 272
Fragments arranged by size:
2570 1288 733 303 272
SacI G_AGCT'C
Cuts at: 0 2048 2862 5166
Size: 2048 814 2304
Fragments arranged by size:
2304 2048 814
SapI GCTCTTCn'nnn_
Cuts at: 0 676 2621 5166
Size: 676 1945 2545
Fragments arranged by size:
2545 1945 676
SexAI A'CCwGG_T
Cuts at: 0 4284 5130 5166
Size: 4284 846 36
Fragments arranged by size:
4284 846 36
SfcI C'TryA_G
Cuts at: 0 430 821 2057 3098 3628 3890 3929 4299
Size: 430 391 1236 1041 530 262 39 370
Cuts at: 4299 4871 5166
Size: 572 295
Fragments arranged by size:
1236 1041 572 530 430 391 370 295
262 39
SmaI CCC'GGG
Cuts at: 0 3952 5166
Size: 3952 1214
SmlI C'TyrA_G
Cuts at: 0 1146 2759 3394 3575 4656 5166
Size: 1146 1613 635 181 1081 510
Fragments arranged by size:
1613 1146 1081 635 510 181
SpeI A'CTAG_T
Cuts at: 0 706 5166
Size: 706 4460
SphI G_CATG'C
Cuts at: 0 348 4674 5166
Size: 348 4326 492
Fragments arranged by size:
4326 492 348
StuI AGG'CCT
Cuts at: 0 1923 5166
Size: 1923 3243
StyI C'CwwG_G
Cuts at: 0 351 465 1163 1647 1918 2742 4120 4757
Size: 351 114 698 484 271 824 1378 637
Cuts at: 4757 5166
Size: 409
Fragments arranged by size:
1378 824 698 637 484 409 351 271
114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1760 2022 2300 2375 3991 5166
Size: 1760 262 278 75 1616 1175
Fragments arranged by size:
1760 1616 1175 278 262 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 846 878 1882 1914 2176 2208 4572 4604
Size: 846 32 1004 32 262 32 2364 32
Cuts at: 4604 5166
Size: 562
Fragments arranged by size:
2364 1004 846 562 262 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1306 1545 5166
Size: 1306 239 3621
Fragments arranged by size:
3621 1306 239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 554 3702 4118 4502 4898 5166
Size: 554 3148 416 384 396 268
Fragments arranged by size:
3148 554 416 396 384 268
XhoI C'TCGA_G
Cuts at: 0 2759 5166
Size: 2759 2407
XmaI C'CCGG_G
Cuts at: 0 3950 5166
Size: 3950 1216
XmnI GAAnn'nnTTC
Cuts at: 0 1680 3108 3570 5166
Size: 1680 1428 462 1596
Fragments arranged by size:
1680 1596 1428 462
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BcgI BciVI BglI BglII BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI
SmlI SpeI SphI StuI StyI TaqII TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AlfI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I CspCI EciI
EcoNI FseI FspI FspAI HindIII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PshAI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SrfI SspI SwaI TatI XbaI ZraI