Restriction map for
pF1KSDA1094
(Linear) (Six-base) MAPSORT of: KIAA1094.seq Check: 2858 from: 1 to: 1614
KIAA1094.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 847 1614
Size: 705 142 767
Fragments arranged by size:
767 705 142
AfeI AGC'GCT
Cuts at: 0 631 1614
Size: 631 983
AlwNI CAG_nnn'CTG
Cuts at: 0 349 1274 1614
Size: 349 925 340
Fragments arranged by size:
925 349 340
ApaI G_GGCC'C
Cuts at: 0 1311 1614
Size: 1311 303
AvaI C'yCGr_G
Cuts at: 0 5 27 1614
Size: 5 22 1587
Fragments arranged by size:
1587 22 5
BanI G'GyrC_C
Cuts at: 0 293 751 1614
Size: 293 458 863
Fragments arranged by size:
863 458 293
BanII G_rGCy'C
Cuts at: 0 42 1311 1614
Size: 42 1269 303
Fragments arranged by size:
1269 303 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 729 1098 1614
Size: 201 528 369 516
Fragments arranged by size:
528 516 369 201
BglI GCCn_nnn'nGGC
Cuts at: 0 928 1614
Size: 928 686
BlpI GC'TnA_GC
Cuts at: 0 94 796 1614
Size: 94 702 818
Fragments arranged by size:
818 702 94
Bme1580I G_kGCm'C
Cuts at: 0 16 1311 1614
Size: 16 1295 303
Fragments arranged by size:
1295 303 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 397 658 1614
Size: 397 261 956
Fragments arranged by size:
956 397 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1138 1614
Size: 461 677 476
Fragments arranged by size:
677 476 461
BsaI GGTCTCn'nnnn_
Cuts at: 0 917 1614
Size: 917 697
BsaBI GATnn'nnATC
Cuts at: 0 1471 1614
Size: 1471 143
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 753 1313 1614
Size: 753 560 301
Fragments arranged by size:
753 560 301
BseYI C'CCAG_C
Cuts at: 0 304 1614
Size: 304 1310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 808 1260 1614
Size: 172 636 452 354
Fragments arranged by size:
636 452 354 172
BsmI GAATG_Cn'
Cuts at: 0 960 1614
Size: 960 654
Bsp1286I G_dGCh'C
Cuts at: 0 16 42 1311 1614
Size: 16 26 1269 303
Fragments arranged by size:
1269 303 26 16
BspHI T'CATG_A
Cuts at: 0 281 461 782 1614
Size: 281 180 321 832
Fragments arranged by size:
832 321 281 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 557 1238 1614
Size: 557 681 376
Fragments arranged by size:
681 557 376
BsrBI CCG'CTC
Cuts at: 0 291 1614
Size: 291 1323
BsrFI r'CCGG_y
Cuts at: 0 182 827 1064 1324 1614
Size: 182 645 237 260 290
Fragments arranged by size:
645 290 260 237 182
BssSI C'ACGA_G
Cuts at: 0 126 1614
Size: 126 1488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 354 1614
Size: 304 50 1260
Fragments arranged by size:
1260 304 50
BstYI r'GATC_y
Cuts at: 0 1388 1614
Size: 1388 226
BtgI C'CryG_G
Cuts at: 0 137 254 1379 1614
Size: 137 117 1125 235
Fragments arranged by size:
1125 235 137 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 987 1614
Size: 987 627
BtsI GCAGTG_nn'
Cuts at: 0 92 155 365 580 1069 1614
Size: 92 63 210 215 489 545
Fragments arranged by size:
545 489 215 210 92 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 157 192 1614
Size: 157 35 1422
Fragments arranged by size:
1422 157 35
EaeI y'GGCC_r
Cuts at: 0 920 1066 1614
Size: 920 146 548
Fragments arranged by size:
920 548 146
EarI CTCTTCn'nnn_
Cuts at: 0 373 877 1253 1614
Size: 373 504 376 361
Fragments arranged by size:
504 376 373 361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 705 847 1138 1614
Size: 461 244 142 291 476
Fragments arranged by size:
476 461 291 244 142
EcoO109I rG'GnC_Cy
Cuts at: 0 31 660 993 1307 1308 1614
Size: 31 629 333 314 1 306
Fragments arranged by size:
629 333 314 306 31 1
FspI TGC'GCA
Cuts at: 0 1445 1614
Size: 1445 169
HaeII r_GCGC'y
Cuts at: 0 206 388 633 1614
Size: 206 182 245 981
Fragments arranged by size:
981 245 206 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 109 141 434 466 1557 1589
Size: 1 32 76 32 293 32 1091 32
Cuts at: 1589 1614
Size: 25
Fragments arranged by size:
1091 293 76 32 32 32 32 25
1
MscI TGG'CCA
Cuts at: 0 922 1614
Size: 922 692
MslI CAynn'nnrTG
Cuts at: 0 259 337 352 358 1614
Size: 259 78 15 6 1256
Fragments arranged by size:
1256 259 78 15 6
MspA1I CmG'CkG
Cuts at: 0 45 560 1394 1614
Size: 45 515 834 220
Fragments arranged by size:
834 515 220 45
NcoI C'CATG_G
Cuts at: 0 254 1379 1614
Size: 254 1125 235
Fragments arranged by size:
1125 254 235
NspI r_CATG'y
Cuts at: 0 1245 1614
Size: 1245 369
PfoI T'CCnGG_A
Cuts at: 0 500 1115 1544 1614
Size: 500 615 429 70
Fragments arranged by size:
615 500 429 70
PpuMI rG'GwC_Cy
Cuts at: 0 660 1614
Size: 660 954
PspOMI G'GGCC_C
Cuts at: 0 1307 1614
Size: 1307 307
SanDI GG'GwC_CC
Cuts at: 0 660 1614
Size: 660 954
SfcI C'TryA_G
Cuts at: 0 190 1457 1500 1614
Size: 190 1267 43 114
Fragments arranged by size:
1267 190 114 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 928 1614
Size: 928 686
SmaI CCC'GGG
Cuts at: 0 29 1614
Size: 29 1585
SpeI A'CTAG_T
Cuts at: 0 276 1614
Size: 276 1338
StyI C'CwwG_G
Cuts at: 0 254 801 926 1379 1614
Size: 254 547 125 453 235
Fragments arranged by size:
547 453 254 235 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 583 1330 1336 1614
Size: 134 449 747 6 278
Fragments arranged by size:
747 449 278 134 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1095 1614
Size: 1095 519
XmaI C'CCGG_G
Cuts at: 0 27 1614
Size: 27 1587
XmnI GAAnn'nnTTC
Cuts at: 0 313 509 1614
Size: 313 196 1105
Fragments arranged by size:
1105 313 196
Enzymes that do cut:
AcuI AfeI AlwNI ApaI AvaI BanI BanII BbsI
BglI BlpI Bme1580I BmrI BpmI BsaI BsaBI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoO109I FspI HaeII Hin4I MscI MslI
MspA1I NcoI NspI PfoI PpuMI PspOMI SanDI SfcI
SfiI SmaI SpeI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI
SnaBI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI ZraI