Restriction map for
pF1KSDA1095
(Linear) (Six-base) MAPSORT of: KIAA1095.seq Check: 2217 from: 1 to: 3198
KIAA1095.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 990 1828 3198
Size: 990 838 1370
Fragments arranged by size:
1370 990 838
AccI GT'mk_AC
Cuts at: 0 3167 3198
Size: 3167 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1842 2497 2863 3198
Size: 67 1775 655 366 335
Fragments arranged by size:
1775 655 366 335 67
AfeI AGC'GCT
Cuts at: 0 310 494 1933 3198
Size: 310 184 1439 1265
Fragments arranged by size:
1439 1265 310 184
AflIII A'CryG_T
Cuts at: 0 395 3198
Size: 395 2803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2218 3198
Size: 2218 980
AlwNI CAG_nnn'CTG
Cuts at: 0 291 360 695 2502 2562 2943 3198
Size: 291 69 335 1807 60 381 255
Fragments arranged by size:
1807 381 335 291 255 69 60
ApoI r'AATT_y
Cuts at: 0 655 896 3198
Size: 655 241 2302
Fragments arranged by size:
2302 655 241
AseI AT'TA_AT
Cuts at: 0 1418 3198
Size: 1418 1780
AvaI C'yCGr_G
Cuts at: 0 304 2262 2364 3198
Size: 304 1958 102 834
Fragments arranged by size:
1958 834 304 102
BamHI G'GATC_C
Cuts at: 0 1143 1910 3198
Size: 1143 767 1288
Fragments arranged by size:
1288 1143 767
BanI G'GyrC_C
Cuts at: 0 2452 3198
Size: 2452 746
BanII G_rGCy'C
Cuts at: 0 194 989 1750 1946 1955 2219 2237 2545
Size: 194 795 761 196 9 264 18 308
Cuts at: 2545 2731 3198
Size: 186 467
Fragments arranged by size:
795 761 467 308 264 196 194 186
18 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 1331 1655 3198
Size: 99 1232 324 1543
Fragments arranged by size:
1543 1232 324 99
BbvCI CC'TCA_GC
Cuts at: 0 1173 3198
Size: 1173 2025
BciVI GTATCCnnnnn_n'
Cuts at: 0 858 3198
Size: 858 2340
BglI GCCn_nnn'nGGC
Cuts at: 0 431 604 3198
Size: 431 173 2594
Fragments arranged by size:
2594 431 173
BglII A'GATC_T
Cuts at: 0 2315 3198
Size: 2315 883
BlpI GC'TnA_GC
Cuts at: 0 688 3198
Size: 688 2510
Bme1580I G_kGCm'C
Cuts at: 0 2455 3198
Size: 2455 743
BmgBI CAC'GTC
Cuts at: 0 33 105 201 375 2202 3198
Size: 33 72 96 174 1827 996
Fragments arranged by size:
1827 996 174 96 72 33
BmtI G_CTAG'C
Cuts at: 0 1622 3198
Size: 1622 1576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 3198
Size: 1188 2010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 502 1264 1270 1498 1603 1969 2074
Size: 94 408 762 6 228 105 366 105
Cuts at: 2074 2110 2332 2937 3198
Size: 36 222 605 261
Fragments arranged by size:
762 605 408 366 261 228 222 105
105 94 36 6
Bpu10I CC'TnA_GC
Cuts at: 0 1173 2098 2725 3198
Size: 1173 925 627 473
Fragments arranged by size:
1173 925 627 473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 211 535 640 1939 2116 2355 2992
Size: 196 15 324 105 1299 177 239 637
Cuts at: 2992 3198
Size: 206
Fragments arranged by size:
1299 637 324 239 206 196 177 105
15
BsaI GGTCTCn'nnnn_
Cuts at: 0 1394 3198
Size: 1394 1804
BsaBI GATnn'nnATC
Cuts at: 0 1148 1342 3115 3198
Size: 1148 194 1773 83
Fragments arranged by size:
1773 1148 194 83
BsaHI Gr'CG_yC
Cuts at: 0 1775 1895 2015 3198
Size: 1775 120 120 1183
Fragments arranged by size:
1775 1183 120 120
BsaWI w'CCGG_w
Cuts at: 0 1310 3198
Size: 1310 1888
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1942 1972 2566 2596 3198
Size: 754 30 1158 30 594 30 602
Fragments arranged by size:
1158 754 602 594 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1177 1252 1600 2352 2941 3198
Size: 1177 75 348 752 589 257
Fragments arranged by size:
1177 752 589 348 257 75
BseYI C'CCAG_C
Cuts at: 0 571 599 1126 1290 2551 2562 2630 3198
Size: 571 28 527 164 1261 11 68 568
Fragments arranged by size:
1261 571 568 527 164 68 28 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 523 1018 1453 1552 1872 2146 3198
Size: 160 363 495 435 99 320 274 1052
Fragments arranged by size:
1052 495 435 363 320 274 160 99
BsiEI CG_ry'CG
Cuts at: 0 174 270 802 1883 2675 3198
Size: 174 96 532 1081 792 523
Fragments arranged by size:
1081 792 532 523 174 96
BsiHKAI G_wGCw'C
Cuts at: 0 194 302 1732 1750 1946 1955 2219 2237
Size: 194 108 1430 18 196 9 264 18
Cuts at: 2237 2731 3198
Size: 494 467
Fragments arranged by size:
1430 494 467 264 196 194 108 18
18 9
BsiWI C'GTAC_G
Cuts at: 0 248 3198
Size: 248 2950
Bsp1286I G_dGCh'C
Cuts at: 0 194 302 989 1732 1750 1946 1955 2219
Size: 194 108 687 743 18 196 9 264
Cuts at: 2219 2237 2455 2545 2731 3198
Size: 18 218 90 186 467
Fragments arranged by size:
743 687 467 264 218 196 194 186
108 90 18 18 9
BspHI T'CATG_A
Cuts at: 0 900 948 2981 3198
Size: 900 48 2033 217
Fragments arranged by size:
2033 900 217 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 990 1574 1828 1859 2585 2984 3198
Size: 990 584 254 31 726 399 214
Fragments arranged by size:
990 726 584 399 254 214 31
BsrBI CCG'CTC
Cuts at: 0 210 2304 2344 2517 3198
Size: 210 2094 40 173 681
Fragments arranged by size:
2094 681 210 173 40
BsrDI GCAATG_nn'
Cuts at: 0 1367 1489 3198
Size: 1367 122 1709
Fragments arranged by size:
1709 1367 122
BsrFI r'CCGG_y
Cuts at: 0 115 797 2017 3198
Size: 115 682 1220 1181
Fragments arranged by size:
1220 1181 682 115
BssHII G'CGCG_C
Cuts at: 0 53 160 328 465 485 512 533 567
Size: 53 107 168 137 20 27 21 34
Cuts at: 567 671 673 2109 2835 3198
Size: 104 2 1436 726 363
Fragments arranged by size:
1436 726 363 168 137 107 104 53
34 27 21 20 2
BssSI C'ACGA_G
Cuts at: 0 1693 2212 3198
Size: 1693 519 986
Fragments arranged by size:
1693 986 519
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 2360 3198
Size: 291 2069 838
Fragments arranged by size:
2069 838 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1420 1797 3198
Size: 1420 377 1401
Fragments arranged by size:
1420 1401 377
BstYI r'GATC_y
Cuts at: 0 1143 1910 2315 2439 2771 2846 3198
Size: 1143 767 405 124 332 75 352
Fragments arranged by size:
1143 767 405 352 332 124 75
BstZ17I GTA'TAC
Cuts at: 0 3168 3198
Size: 3168 30
BtgI C'CryG_G
Cuts at: 0 168 608 2390 2675 3135 3198
Size: 168 440 1782 285 460 63
Fragments arranged by size:
1782 460 440 285 168 63
BtsI GCAGTG_nn'
Cuts at: 0 477 3198
Size: 477 2721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 328 363 3198
Size: 328 35 2835
Fragments arranged by size:
2835 328 35
DraIII CAC_nnn'GTG
Cuts at: 0 95 1304 2946 3198
Size: 95 1209 1642 252
Fragments arranged by size:
1642 1209 252 95
EaeI y'GGCC_r
Cuts at: 0 99 182 267 351 409 474 605 701
Size: 99 83 85 84 58 65 131 96
Cuts at: 701 795 799 1880 2672 3198
Size: 94 4 1081 792 526
Fragments arranged by size:
1081 792 526 131 99 96 94 85
84 83 65 58 4
EagI C'GGCC_G
Cuts at: 0 267 799 1880 2672 3198
Size: 267 532 1081 792 526
Fragments arranged by size:
1081 792 532 526 267
EarI CTCTTCn'nnn_
Cuts at: 0 1439 1463 1963 2063 2849 3076 3198
Size: 1439 24 500 100 786 227 122
Fragments arranged by size:
1439 786 500 227 122 100 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1731 2362 3198
Size: 1731 631 836
Fragments arranged by size:
1731 836 631
EcoICRI GAG'CTC
Cuts at: 0 192 1748 1944 1953 2217 2235 2729 3198
Size: 192 1556 196 9 264 18 494 469
Fragments arranged by size:
1556 494 469 264 196 192 18 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 94 502 1264 1270 1498 1603 1842
Size: 67 27 408 762 6 228 105 239
Cuts at: 1842 1969 2074 2110 2332 2497 2863 2937 3198
Size: 127 105 36 222 165 366 74 261
Fragments arranged by size:
762 408 366 261 239 228 222 165
127 105 105 74 67 36 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2947 3198
Size: 2947 251
EcoO109I rG'GnC_Cy
Cuts at: 0 69 78 2721 3198
Size: 69 9 2643 477
Fragments arranged by size:
2643 477 69 9
EcoRV GAT'ATC
Cuts at: 0 2226 3198
Size: 2226 972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3114 3198
Size: 3114 84
FseI GG_CCGG'CC
Cuts at: 0 801 3198
Size: 801 2397
FspI TGC'GCA
Cuts at: 0 2077 2206 3198
Size: 2077 129 992
Fragments arranged by size:
2077 992 129
HaeII r_GCGC'y
Cuts at: 0 219 312 496 550 565 1935 1976 2270
Size: 219 93 184 54 15 1370 41 294
Cuts at: 2270 2573 2913 3198
Size: 303 340 285
Fragments arranged by size:
1370 340 303 294 285 219 184 93
54 41 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 811 843 1107 1139 1732 1764
Size: 754 32 25 32 264 32 593 32
Cuts at: 1764 2201 2233 2713 2745 3198
Size: 437 32 480 32 453
Fragments arranged by size:
754 593 480 453 437 264 32 32
32 32 32 32 25
HincII GTy'rAC
Cuts at: 0 861 921 3198
Size: 861 60 2277
Fragments arranged by size:
2277 861 60
HindIII A'AGCT_T
Cuts at: 0 965 3198
Size: 965 2233
MluI A'CGCG_T
Cuts at: 0 395 3198
Size: 395 2803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 1814 2273 2723 2978 3198
Size: 1709 105 459 450 255 220
Fragments arranged by size:
1709 459 450 255 220 105
MslI CAynn'nnrTG
Cuts at: 0 994 1114 1576 2200 3198
Size: 994 120 462 624 998
Fragments arranged by size:
998 994 624 462 120
MspA1I CmG'CkG
Cuts at: 0 84 152 170 291 422 603 610 695
Size: 84 68 18 121 131 181 7 85
Cuts at: 695 1056 1518 1706 1797 1844 2171 2360 2655
Size: 361 462 188 91 47 327 189 295
Cuts at: 2655 2864 2964 3198
Size: 209 100 234
Fragments arranged by size:
462 361 327 295 234 209 189 188
181 131 121 100 91 85 84 68
47 18 7
NaeI GCC'GGC
Cuts at: 0 117 799 2019 3198
Size: 117 682 1220 1179
Fragments arranged by size:
1220 1179 682 117
NgoMIV G'CCGG_C
Cuts at: 0 115 797 2017 3198
Size: 115 682 1220 1181
Fragments arranged by size:
1220 1181 682 115
NheI G'CTAG_C
Cuts at: 0 1618 3198
Size: 1618 1580
NspI r_CATG'y
Cuts at: 0 390 1581 2652 2715 3198
Size: 390 1191 1071 63 483
Fragments arranged by size:
1191 1071 483 390 63
PasI CC'CwG_GG
Cuts at: 0 131 774 3198
Size: 131 643 2424
Fragments arranged by size:
2424 643 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 960 2502 2562 3198
Size: 960 1542 60 636
Fragments arranged by size:
1542 960 636 60
PfoI T'CCnGG_A
Cuts at: 0 71 1946 2152 2237 2849 3198
Size: 71 1875 206 85 612 349
Fragments arranged by size:
1875 612 349 206 85 71
PpuMI rG'GwC_Cy
Cuts at: 0 69 78 2721 3198
Size: 69 9 2643 477
Fragments arranged by size:
2643 477 69 9
PshAI GACnn'nnGTC
Cuts at: 0 748 3198
Size: 748 2450
PspXI vC'TCGA_Gb
Cuts at: 0 304 2262 3198
Size: 304 1958 936
Fragments arranged by size:
1958 936 304
PstI C_TGCA'G
Cuts at: 0 287 629 876 1631 1825 3198
Size: 287 342 247 755 194 1373
Fragments arranged by size:
1373 755 342 287 247 194
PvuII CAG'CTG
Cuts at: 0 152 291 603 1056 1518 2360 2655 3198
Size: 152 139 312 453 462 842 295 543
Fragments arranged by size:
842 543 462 453 312 295 152 139
RsrII CG'GwC_CG
Cuts at: 0 1718 3198
Size: 1718 1480
SacI G_AGCT'C
Cuts at: 0 194 1750 1946 1955 2219 2237 2731 3198
Size: 194 1556 196 9 264 18 494 467
Fragments arranged by size:
1556 494 467 264 196 194 18 9
SacII CC_GC'GG
Cuts at: 0 171 611 3198
Size: 171 440 2587
Fragments arranged by size:
2587 440 171
SapI GCTCTTCn'nnn_
Cuts at: 0 1463 1963 2063 2849 3198
Size: 1463 500 100 786 349
Fragments arranged by size:
1463 786 500 349 100
SexAI A'CCwGG_T
Cuts at: 0 2942 3198
Size: 2942 256
SfcI C'TryA_G
Cuts at: 0 283 625 872 1266 1627 1821 2463 3198
Size: 283 342 247 394 361 194 642 735
Fragments arranged by size:
735 642 394 361 342 283 247 194
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 604 3198
Size: 604 2594
SmlI C'TyrA_G
Cuts at: 0 211 226 304 550 619 1954 2131 2262
Size: 211 15 78 246 69 1335 177 131
Cuts at: 2262 2334 3007 3198
Size: 72 673 191
Fragments arranged by size:
1335 673 246 211 191 177 131 78
72 69 15
SspI AAT'ATT
Cuts at: 0 786 3198
Size: 786 2412
StuI AGG'CCT
Cuts at: 0 890 1506 3198
Size: 890 616 1692
Fragments arranged by size:
1692 890 616
StyI C'CwwG_G
Cuts at: 0 878 980 1018 1376 1835 2954 3198
Size: 878 102 38 358 459 1119 244
Fragments arranged by size:
1119 878 459 358 244 102 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 1735 3198
Size: 123 1612 1463
Fragments arranged by size:
1612 1463 123
TatI w'GTAC_w
Cuts at: 0 1991 3198
Size: 1991 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 226 258 1942 1974 2424 2456
Size: 34 32 160 32 1684 32 450 32
Cuts at: 2456 3198
Size: 742
Fragments arranged by size:
1684 742 450 160 34 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2499 2559 3198
Size: 2499 60 639
Fragments arranged by size:
2499 639 60
XhoI C'TCGA_G
Cuts at: 0 304 2262 3198
Size: 304 1958 936
Fragments arranged by size:
1958 936 304
XmnI GAAnn'nnTTC
Cuts at: 0 655 3097 3198
Size: 655 2442 101
Fragments arranged by size:
2442 655 101
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApoI
AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
BstZ17I BtgI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII MluI MmeI MslI
MspA1I NaeI NgoMIV NheI NspI PasI PflMI PfoI
PpuMI PshAI PspXI PstI PvuII RsrII SacI SacII
SapI SexAI SfcI SfiI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI
BclI BfrBI BmrI BsaAI BsmI BsmBI BspEI BsrGI
BstBI BstEII Bsu36I BtgZI ClaI DraI DrdI EcoRI
FspAI HpaI KasI KpnI MfeI MscI NarI NcoI
NdeI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PspOMI PsrI PvuI SalI SanDI SbfI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XmaI ZraI