Restriction map for
pF1KA1097
(Linear) (Six-base) MAPSORT of: KIAA1097.seq Check: 4102 from: 1 to: 2733
KIAA1097.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1082 1168 2733
Size: 1082 86 1565
Fragments arranged by size:
1565 1082 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1058 1115 1816 2012 2104 2621 2666
Size: 947 111 57 701 196 92 517 45
Cuts at: 2666 2733
Size: 67
Fragments arranged by size:
947 701 517 196 111 92 67 57
45
AhdI GACnn_n'nnGTC
Cuts at: 0 1389 2314 2733
Size: 1389 925 419
Fragments arranged by size:
1389 925 419
AleI CACnn'nnGTG
Cuts at: 0 1527 2733
Size: 1527 1206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2476 2733
Size: 2476 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2099 2131 2733
Size: 2099 32 602
Fragments arranged by size:
2099 602 32
AlwNI CAG_nnn'CTG
Cuts at: 0 646 1374 2587 2733
Size: 646 728 1213 146
Fragments arranged by size:
1213 728 646 146
ApoI r'AATT_y
Cuts at: 0 727 1032 1045 1969 2088 2152 2393 2613
Size: 727 305 13 924 119 64 241 220
Cuts at: 2613 2733
Size: 120
Fragments arranged by size:
924 727 305 241 220 120 119 64
13
AseI AT'TA_AT
Cuts at: 0 1022 2733
Size: 1022 1711
AvaI C'yCGr_G
Cuts at: 0 1402 2733
Size: 1402 1331
BanI G'GyrC_C
Cuts at: 0 1257 2733
Size: 1257 1476
BbsI GAAGACnn'nnnn_
Cuts at: 0 839 1451 2252 2733
Size: 839 612 801 481
Fragments arranged by size:
839 801 612 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1498 1532 2733
Size: 1498 34 1201
Fragments arranged by size:
1498 1201 34
BclI T'GATC_A
Cuts at: 0 1977 2733
Size: 1977 756
BglII A'GATC_T
Cuts at: 0 1416 2733
Size: 1416 1317
BlpI GC'TnA_GC
Cuts at: 0 1454 2733
Size: 1454 1279
BmrI ACTGGGnnnn_n'
Cuts at: 0 359 2733
Size: 359 2374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 2420 2498 2733
Size: 151 2269 78 235
Fragments arranged by size:
2269 235 151 78
Bpu10I CC'TnA_GC
Cuts at: 0 1847 2569 2733
Size: 1847 722 164
Fragments arranged by size:
1847 722 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 2733
Size: 707 2026
BsaI GGTCTCn'nnnn_
Cuts at: 0 1399 2733
Size: 1399 1334
BsaBI GATnn'nnATC
Cuts at: 0 2507 2733
Size: 2507 226
BsaWI w'CCGG_w
Cuts at: 0 2664 2733
Size: 2664 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2099 2129 2733
Size: 2099 30 604
Fragments arranged by size:
2099 604 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 377 832 2088 2733
Size: 377 455 1256 645
Fragments arranged by size:
1256 645 455 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 2733
Size: 1387 1346
BsmI GAATG_Cn'
Cuts at: 0 501 1461 2733
Size: 501 960 1272
Fragments arranged by size:
1272 960 501
BspHI T'CATG_A
Cuts at: 0 756 2733
Size: 756 1977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 591 2285 2733
Size: 591 1694 448
Fragments arranged by size:
1694 591 448
BsrDI GCAATG_nn'
Cuts at: 0 301 2733
Size: 301 2432
BsrGI T'GTAC_A
Cuts at: 0 1667 2010 2327 2733
Size: 1667 343 317 406
Fragments arranged by size:
1667 406 343 317
BstBI TT'CG_AA
Cuts at: 0 12 1156 2733
Size: 12 1144 1577
Fragments arranged by size:
1577 1144 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 119 1529 2733
Size: 119 1410 1204
Fragments arranged by size:
1410 1204 119
BstYI r'GATC_y
Cuts at: 0 643 795 1178 1416 1827 2508 2733
Size: 643 152 383 238 411 681 225
Fragments arranged by size:
681 643 411 383 238 225 152
BtsI GCAGTG_nn'
Cuts at: 0 1375 2470 2733
Size: 1375 1095 263
Fragments arranged by size:
1375 1095 263
DraI TTT'AAA
Cuts at: 0 357 2733
Size: 357 2376
EaeI y'GGCC_r
Cuts at: 0 1246 2589 2733
Size: 1246 1343 144
Fragments arranged by size:
1343 1246 144
EarI CTCTTCn'nnn_
Cuts at: 0 239 1552 2041 2051 2733
Size: 239 1313 489 10 682
Fragments arranged by size:
1313 682 489 239 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2371 2733
Size: 2371 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 947 1058 1115 1816 2012 2104 2420
Size: 151 796 111 57 701 196 92 316
Cuts at: 2420 2498 2621 2666 2733
Size: 78 123 45 67
Fragments arranged by size:
796 701 316 196 151 123 111 92
78 67 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1232 2733
Size: 1232 1501
EcoO109I rG'GnC_Cy
Cuts at: 0 2518 2641 2733
Size: 2518 123 92
Fragments arranged by size:
2518 123 92
EcoRI G'AATT_C
Cuts at: 0 727 2733
Size: 727 2006
EcoRV GAT'ATC
Cuts at: 0 2084 2733
Size: 2084 649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 457 748 1639 2733
Size: 457 291 891 1094
Fragments arranged by size:
1094 891 457 291
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 1048 1054 1080 1086 1375 1407
Size: 847 32 169 6 26 6 289 32
Cuts at: 1407 1863 1870 1895 1902 2250 2282 2300 2332
Size: 456 7 25 7 348 32 18 32
Cuts at: 2332 2733
Size: 401
Fragments arranged by size:
847 456 401 348 289 169 32 32
32 32 26 25 18 7 7 6
6
HincII GTy'rAC
Cuts at: 0 1083 2319 2733
Size: 1083 1236 414
Fragments arranged by size:
1236 1083 414
HindIII A'AGCT_T
Cuts at: 0 2024 2733
Size: 2024 709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 827 1501 2733
Size: 715 112 674 1232
Fragments arranged by size:
1232 715 674 112
MscI TGG'CCA
Cuts at: 0 1248 2591 2733
Size: 1248 1343 142
Fragments arranged by size:
1343 1248 142
MslI CAynn'nnrTG
Cuts at: 0 1157 1527 2213 2458 2733
Size: 1157 370 686 245 275
Fragments arranged by size:
1157 686 370 275 245
MspA1I CmG'CkG
Cuts at: 0 2314 2733
Size: 2314 419
NdeI CA'TA_TG
Cuts at: 0 147 1515 1875 2733
Size: 147 1368 360 858
Fragments arranged by size:
1368 858 360 147
NspI r_CATG'y
Cuts at: 0 128 2733
Size: 128 2605
PflMI CCAn_nnn'nTGG
Cuts at: 0 1435 2733
Size: 1435 1298
PfoI T'CCnGG_A
Cuts at: 0 347 2733
Size: 347 2386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2099 2131 2733
Size: 2099 32 602
Fragments arranged by size:
2099 602 32
PpuMI rG'GwC_Cy
Cuts at: 0 2518 2733
Size: 2518 215
PstI C_TGCA'G
Cuts at: 0 2624 2733
Size: 2624 109
PvuII CAG'CTG
Cuts at: 0 2314 2733
Size: 2314 419
SalI G'TCGA_C
Cuts at: 0 1081 2733
Size: 1081 1652
SapI GCTCTTCn'nnn_
Cuts at: 0 2041 2733
Size: 2041 692
SexAI A'CCwGG_T
Cuts at: 0 2249 2733
Size: 2249 484
SfcI C'TryA_G
Cuts at: 0 1484 2040 2620 2733
Size: 1484 556 580 113
Fragments arranged by size:
1484 580 556 113
SmlI C'TyrA_G
Cuts at: 0 722 1402 2733
Size: 722 680 1331
Fragments arranged by size:
1331 722 680
SspI AAT'ATT
Cuts at: 0 474 2733
Size: 474 2259
StyI C'CwwG_G
Cuts at: 0 78 105 2733
Size: 78 27 2628
Fragments arranged by size:
2628 78 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2714 2733
Size: 2714 19
TatI w'GTAC_w
Cuts at: 0 1667 1719 2010 2327 2733
Size: 1667 52 291 317 406
Fragments arranged by size:
1667 406 317 291 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 635 667 1163 1195 2733
Size: 635 32 496 32 1538
Fragments arranged by size:
1538 635 496 32 32
XbaI T'CTAG_A
Cuts at: 0 913 2733
Size: 913 1820
XhoI C'TCGA_G
Cuts at: 0 1402 2733
Size: 1402 1331
XmnI GAAnn'nnTTC
Cuts at: 0 731 2733
Size: 731 2002
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AloI AlwNI ApoI
AseI AvaI BanI BbsI BcgI BclI BglII BlpI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BsgI BsmI BspHI BspMI BsrDI BsrGI BstBI
BstXI BstYI BtsI DraI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NdeI NspI PflMI PfoI
PpiI PpuMI PstI PvuII SalI SapI SexAI SfcI
SmlI SspI StyI TaqII TatI TstI XbaI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII
BbeI BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmtI
BplI BsaAI BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EcoICRI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XcmI XmaI ZraI