Restriction map for
pF1KSDA1098
(Linear) (Six-base) MAPSORT of: KIAA1098.seq Check: 9244 from: 1 to: 1479
KIAA1098.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 175 984 1479
Size: 144 31 809 495
Fragments arranged by size:
809 495 144 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1479
Size: 894 585
AflIII A'CryG_T
Cuts at: 0 17 1119 1479
Size: 17 1102 360
Fragments arranged by size:
1102 360 17
AhdI GACnn_n'nnGTC
Cuts at: 0 189 1479
Size: 189 1290
AleI CACnn'nnGTG
Cuts at: 0 1454 1479
Size: 1454 25
ApaLI G'TGCA_C
Cuts at: 0 1403 1479
Size: 1403 76
AvaI C'yCGr_G
Cuts at: 0 25 337 1134 1179 1479
Size: 25 312 797 45 300
Fragments arranged by size:
797 312 300 45 25
BamHI G'GATC_C
Cuts at: 0 557 1479
Size: 557 922
BanI G'GyrC_C
Cuts at: 0 392 1023 1411 1479
Size: 392 631 388 68
Fragments arranged by size:
631 392 388 68
BanII G_rGCy'C
Cuts at: 0 856 1042 1063 1479
Size: 856 186 21 416
Fragments arranged by size:
856 416 186 21
BbeI G_GCGC'C
Cuts at: 0 1027 1415 1479
Size: 1027 388 64
Fragments arranged by size:
1027 388 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 1041 1479
Size: 1041 438
BbvCI CC'TCA_GC
Cuts at: 0 1142 1479
Size: 1142 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 773 807 813 847 1479
Size: 773 34 6 34 632
Fragments arranged by size:
773 632 34 34 6
BglI GCCn_nnn'nGGC
Cuts at: 0 487 1037 1479
Size: 487 550 442
Fragments arranged by size:
550 487 442
BlpI GC'TnA_GC
Cuts at: 0 322 1479
Size: 322 1157
Bme1580I G_kGCm'C
Cuts at: 0 395 1407 1479
Size: 395 1012 72
Fragments arranged by size:
1012 395 72
BmgBI CAC'GTC
Cuts at: 0 18 1250 1479
Size: 18 1232 229
Fragments arranged by size:
1232 229 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 1479
Size: 166 1313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 1479
Size: 1167 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1122 1315 1406 1479
Size: 1122 193 91 73
Fragments arranged by size:
1122 193 91 73
Bpu10I CC'TnA_GC
Cuts at: 0 1142 1479
Size: 1142 337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1479
Size: 612 867
BsaAI yAC'GTr
Cuts at: 0 1120 1479
Size: 1120 359
BsaHI Gr'CG_yC
Cuts at: 0 1024 1412 1479
Size: 1024 388 67
Fragments arranged by size:
1024 388 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 838 868 1282 1312 1479
Size: 340 30 468 30 414 30 167
Fragments arranged by size:
468 414 340 167 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 268 616 646 700 1008 1318 1479
Size: 64 204 348 30 54 308 310 161
Fragments arranged by size:
348 310 308 204 161 64 54 30
BseYI C'CCAG_C
Cuts at: 0 143 1035 1242 1479
Size: 143 892 207 237
Fragments arranged by size:
892 237 207 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 292 421 462 777 1012 1479
Size: 172 120 129 41 315 235 467
Fragments arranged by size:
467 315 235 172 129 120 41
BsiHKAI G_wGCw'C
Cuts at: 0 1407 1479
Size: 1407 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 682 1479
Size: 682 797
Bsp1286I G_dGCh'C
Cuts at: 0 395 856 1042 1063 1407 1479
Size: 395 461 186 21 344 72
Fragments arranged by size:
461 395 344 186 72 21
BspHI T'CATG_A
Cuts at: 0 758 1479
Size: 758 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 175 212 984 1479
Size: 144 31 37 772 495
Fragments arranged by size:
772 495 144 37 31
BsrBI CCG'CTC
Cuts at: 0 6 38 717 1479
Size: 6 32 679 762
Fragments arranged by size:
762 679 32 6
BsrFI r'CCGG_y
Cuts at: 0 405 1479
Size: 405 1074
BsrGI T'GTAC_A
Cuts at: 0 1349 1479
Size: 1349 130
BssHII G'CGCG_C
Cuts at: 0 266 268 1479
Size: 266 2 1211
Fragments arranged by size:
1211 266 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 462 1479
Size: 399 63 1017
Fragments arranged by size:
1017 399 63
BstYI r'GATC_y
Cuts at: 0 557 1479
Size: 557 922
BtgI C'CryG_G
Cuts at: 0 123 312 623 797 978 1479
Size: 123 189 311 174 181 501
Fragments arranged by size:
501 311 189 181 174 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 498 1479
Size: 498 981
BtsI GCAGTG_nn'
Cuts at: 0 416 460 1224 1338 1479
Size: 416 44 764 114 141
Fragments arranged by size:
764 416 141 114 44
ClaI AT'CG_AT
Cuts at: 0 833 1479
Size: 833 646
DraIII CAC_nnn'GTG
Cuts at: 0 399 1230 1479
Size: 399 831 249
Fragments arranged by size:
831 399 249
EaeI y'GGCC_r
Cuts at: 0 108 626 656 1265 1479
Size: 108 518 30 609 214
Fragments arranged by size:
609 518 214 108 30
EarI CTCTTCn'nnn_
Cuts at: 0 334 793 1479
Size: 334 459 686
Fragments arranged by size:
686 459 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1122 1315 1406 1479
Size: 894 228 193 91 73
Fragments arranged by size:
894 228 193 91 73
FspI TGC'GCA
Cuts at: 0 208 1435 1479
Size: 208 1227 44
Fragments arranged by size:
1227 208 44
HaeII r_GCGC'y
Cuts at: 0 501 1027 1142 1338 1415 1479
Size: 501 526 115 196 77 64
Fragments arranged by size:
526 501 196 115 77 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1162 1194 1479
Size: 175 32 955 32 285
Fragments arranged by size:
955 285 175 32 32
HindIII A'AGCT_T
Cuts at: 0 577 1479
Size: 577 902
KasI G'GCGC_C
Cuts at: 0 1023 1411 1479
Size: 1023 388 68
Fragments arranged by size:
1023 388 68
MscI TGG'CCA
Cuts at: 0 1267 1479
Size: 1267 212
MslI CAynn'nnrTG
Cuts at: 0 1454 1479
Size: 1454 25
MspA1I CmG'CkG
Cuts at: 0 125 140 314 934 1160 1479
Size: 125 15 174 620 226 319
Fragments arranged by size:
620 319 226 174 125 15
NarI GG'CG_CC
Cuts at: 0 1024 1412 1479
Size: 1024 388 67
Fragments arranged by size:
1024 388 67
NcoI C'CATG_G
Cuts at: 0 623 797 978 1479
Size: 623 174 181 501
Fragments arranged by size:
623 501 181 174
NruI TCG'CGA
Cuts at: 0 584 1479
Size: 584 895
NspI r_CATG'y
Cuts at: 0 460 828 1479
Size: 460 368 651
Fragments arranged by size:
651 460 368
PspXI vC'TCGA_Gb
Cuts at: 0 337 1479
Size: 337 1142
PstI C_TGCA'G
Cuts at: 0 725 863 1097 1479
Size: 725 138 234 382
Fragments arranged by size:
725 382 234 138
PvuII CAG'CTG
Cuts at: 0 934 1160 1479
Size: 934 226 319
Fragments arranged by size:
934 319 226
SacII CC_GC'GG
Cuts at: 0 126 315 1479
Size: 126 189 1164
Fragments arranged by size:
1164 189 126
SexAI A'CCwGG_T
Cuts at: 0 230 1479
Size: 230 1249
SfcI C'TryA_G
Cuts at: 0 721 859 1093 1479
Size: 721 138 234 386
Fragments arranged by size:
721 386 234 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 487 1479
Size: 487 992
SfoI GGC'GCC
Cuts at: 0 1025 1413 1479
Size: 1025 388 66
Fragments arranged by size:
1025 388 66
SmaI CCC'GGG
Cuts at: 0 27 1479
Size: 27 1452
SmlI C'TyrA_G
Cuts at: 0 337 591 1479
Size: 337 254 888
Fragments arranged by size:
888 337 254
SphI G_CATG'C
Cuts at: 0 460 828 1479
Size: 460 368 651
Fragments arranged by size:
651 460 368
StuI AGG'CCT
Cuts at: 0 481 1479
Size: 481 998
StyI C'CwwG_G
Cuts at: 0 476 623 797 978 1083 1479
Size: 476 147 174 181 105 396
Fragments arranged by size:
476 396 181 174 147 105
TatI w'GTAC_w
Cuts at: 0 1349 1479
Size: 1349 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 1282 1314 1479
Size: 340 32 910 32 165
Fragments arranged by size:
910 340 165 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 91 1479
Size: 91 1388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1366 1479
Size: 1254 112 113
Fragments arranged by size:
1254 113 112
XhoI C'TCGA_G
Cuts at: 0 337 1479
Size: 337 1142
XmaI C'CCGG_G
Cuts at: 0 25 1479
Size: 25 1454
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstYI
BtgI BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI
FspI HaeII Hin4I HindIII KasI MscI MslI MspA1I
NarI NcoI NruI NspI PspXI PstI PvuII SacII
SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BglII BmtI BsaI BsaBI
BsaWI BsiEI BsiWI BsmI BspEI BsrDI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspAI HincII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TaqII XbaI XmnI ZraI