Restriction map for
pF1KSDA1099
(Linear) (Six-base) MAPSORT of: KIAA1099.seq Check: 6789 from: 1 to: 2412
KIAA1099.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 765 2054 2412
Size: 765 1289 358
Fragments arranged by size:
1289 765 358
AatII G_ACGT'C
Cuts at: 0 2225 2412
Size: 2225 187
AccI GT'mk_AC
Cuts at: 0 1943 2412
Size: 1943 469
Acc65I G'GTAC_C
Cuts at: 0 269 2412
Size: 269 2143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1502 2412
Size: 414 1088 910
Fragments arranged by size:
1088 910 414
AfeI AGC'GCT
Cuts at: 0 55 2329 2412
Size: 55 2274 83
Fragments arranged by size:
2274 83 55
AflIII A'CryG_T
Cuts at: 0 628 2412
Size: 628 1784
AgeI A'CCGG_T
Cuts at: 0 266 2412
Size: 266 2146
AhdI GACnn_n'nnGTC
Cuts at: 0 194 1467 2412
Size: 194 1273 945
Fragments arranged by size:
1273 945 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 797 2173 2314 2412
Size: 797 1376 141 98
Fragments arranged by size:
1376 797 141 98
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1958 1990 2412
Size: 1958 32 422
Fragments arranged by size:
1958 422 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 711 1602 1904 2046 2202 2412
Size: 18 693 891 302 142 156 210
Fragments arranged by size:
891 693 302 210 156 142 18
ApaI G_GGCC'C
Cuts at: 0 382 2412
Size: 382 2030
ApaLI G'TGCA_C
Cuts at: 0 262 612 787 2412
Size: 262 350 175 1625
Fragments arranged by size:
1625 350 262 175
ApoI r'AATT_y
Cuts at: 0 1497 2412
Size: 1497 915
AvaI C'yCGr_G
Cuts at: 0 558 1232 1817 2108 2231 2412
Size: 558 674 585 291 123 181
Fragments arranged by size:
674 585 558 291 181 123
BamHI G'GATC_C
Cuts at: 0 1787 1973 2412
Size: 1787 186 439
Fragments arranged by size:
1787 439 186
BanI G'GyrC_C
Cuts at: 0 269 1804 2397 2412
Size: 269 1535 593 15
Fragments arranged by size:
1535 593 269 15
BanII G_rGCy'C
Cuts at: 0 215 382 1764 1859 2412
Size: 215 167 1382 95 553
Fragments arranged by size:
1382 553 215 167 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 654 2412
Size: 420 234 1758
Fragments arranged by size:
1758 420 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 613 647 2412
Size: 613 34 1765
Fragments arranged by size:
1765 613 34
BclI T'GATC_A
Cuts at: 0 362 2412
Size: 362 2050
BglI GCCn_nnn'nGGC
Cuts at: 0 28 40 1472 2272 2412
Size: 28 12 1432 800 140
Fragments arranged by size:
1432 800 140 28 12
Bme1580I G_kGCm'C
Cuts at: 0 266 283 382 616 791 2402 2412
Size: 266 17 99 234 175 1611 10
Fragments arranged by size:
1611 266 234 175 99 17 10
BmgBI CAC'GTC
Cuts at: 0 147 629 1241 2298 2412
Size: 147 482 612 1057 114
Fragments arranged by size:
1057 612 482 147 114
BmrI ACTGGGnnnn_n'
Cuts at: 0 1139 1352 1749 2412
Size: 1139 213 397 663
Fragments arranged by size:
1139 663 397 213
BmtI G_CTAG'C
Cuts at: 0 1894 2412
Size: 1894 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1018 1245 1335 1350 2262 2412
Size: 121 897 227 90 15 912 150
Fragments arranged by size:
912 897 227 150 121 90 15
Bpu10I CC'TnA_GC
Cuts at: 0 1782 2412
Size: 1782 630
BsaI GGTCTCn'nnnn_
Cuts at: 0 1717 2119 2412
Size: 1717 402 293
Fragments arranged by size:
1717 402 293
BsaAI yAC'GTr
Cuts at: 0 617 2412
Size: 617 1795
BsaHI Gr'CG_yC
Cuts at: 0 575 1571 2222 2412
Size: 575 996 651 190
Fragments arranged by size:
996 651 575 190
BsaWI w'CCGG_w
Cuts at: 0 266 303 1685 1793 2375 2412
Size: 266 37 1382 108 582 37
Fragments arranged by size:
1382 582 266 108 37 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 379 409 820 850 2207 2237
Size: 67 30 282 30 411 30 1357 30
Cuts at: 2237 2412
Size: 175
Fragments arranged by size:
1357 411 282 175 67 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 310 828 1576 2412
Size: 127 183 518 748 836
Fragments arranged by size:
836 748 518 183 127
BseYI C'CCAG_C
Cuts at: 0 748 856 1332 2412
Size: 748 108 476 1080
Fragments arranged by size:
1080 748 476 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2004 2412
Size: 2004 408
BsiEI CG_ry'CG
Cuts at: 0 1029 2148 2412
Size: 1029 1119 264
Fragments arranged by size:
1119 1029 264
BsiHKAI G_wGCw'C
Cuts at: 0 146 215 266 616 791 1859 2339 2412
Size: 146 69 51 350 175 1068 480 73
Fragments arranged by size:
1068 480 350 175 146 73 69 51
BsmI GAATG_Cn'
Cuts at: 0 1783 2412
Size: 1783 629
BsmBI CGTCTCn'nnnn_
Cuts at: 0 619 2135 2412
Size: 619 1516 277
Fragments arranged by size:
1516 619 277
Bsp1286I G_dGCh'C
Cuts at: 0 146 215 266 283 382 616 791 1764
Size: 146 69 51 17 99 234 175 973
Cuts at: 1764 1859 2339 2402 2412
Size: 95 480 63 10
Fragments arranged by size:
973 480 234 175 146 99 95 69
63 51 17 10
BspEI T'CCGG_A
Cuts at: 0 303 1685 2412
Size: 303 1382 727
Fragments arranged by size:
1382 727 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 765 2054 2081 2137 2412
Size: 305 460 1289 27 56 275
Fragments arranged by size:
1289 460 305 275 56 27
BsrBI CCG'CTC
Cuts at: 0 1025 1566 2412
Size: 1025 541 846
Fragments arranged by size:
1025 846 541
BsrFI r'CCGG_y
Cuts at: 0 117 266 938 2412
Size: 117 149 672 1474
Fragments arranged by size:
1474 672 149 117
BssSI C'ACGA_G
Cuts at: 0 501 2412
Size: 501 1911
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1778 2049 2412
Size: 1778 271 363
Fragments arranged by size:
1778 363 271
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 2412
Size: 408 2004
BstYI r'GATC_y
Cuts at: 0 47 134 1598 1787 1973 2412
Size: 47 87 1464 189 186 439
Fragments arranged by size:
1464 439 189 186 87 47
BtgI C'CryG_G
Cuts at: 0 1667 2241 2412
Size: 1667 574 171
Fragments arranged by size:
1667 574 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 135 2412
Size: 135 2277
BtsI GCAGTG_nn'
Cuts at: 0 896 1475 2394 2412
Size: 896 579 919 18
Fragments arranged by size:
919 896 579 18
ClaI AT'CG_AT
Cuts at: 0 569 884 2412
Size: 569 315 1528
Fragments arranged by size:
1528 569 315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2370 2405 2412
Size: 2370 35 7
Fragments arranged by size:
2370 35 7
DraIII CAC_nnn'GTG
Cuts at: 0 1115 1713 2412
Size: 1115 598 699
Fragments arranged by size:
1115 699 598
DrdI GACnn_nn'nnGTC
Cuts at: 0 1576 2412
Size: 1576 836
EaeI y'GGCC_r
Cuts at: 0 20 239 485 1026 1527 1604 1846 2081
Size: 20 219 246 541 501 77 242 235
Cuts at: 2081 2145 2342 2412
Size: 64 197 70
Fragments arranged by size:
541 501 246 242 235 219 197 77
70 64 20
EagI C'GGCC_G
Cuts at: 0 1026 2145 2412
Size: 1026 1119 267
Fragments arranged by size:
1119 1026 267
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1904 1952 2005 2412
Size: 1216 688 48 53 407
Fragments arranged by size:
1216 688 407 53 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1941 2412
Size: 1941 471
EcoICRI GAG'CTC
Cuts at: 0 213 1857 2412
Size: 213 1644 555
Fragments arranged by size:
1644 555 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 414 1018 1245 1335 1350 1502 2262
Size: 121 293 604 227 90 15 152 760
Cuts at: 2262 2412
Size: 150
Fragments arranged by size:
760 604 293 227 152 150 121 90
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1285 2412
Size: 1285 1127
EcoO109I rG'GnC_Cy
Cuts at: 0 378 379 721 2070 2412
Size: 378 1 342 1349 342
Fragments arranged by size:
1349 378 342 342 1
FspI TGC'GCA
Cuts at: 0 1251 2412
Size: 1251 1161
HaeII r_GCGC'y
Cuts at: 0 57 939 2158 2331 2412
Size: 57 882 1219 173 81
Fragments arranged by size:
1219 882 173 81 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 180 212 820 852 1125 1157 1453 1485
Size: 180 32 608 32 273 32 296 32
Cuts at: 1485 1716 1748 2207 2239 2412
Size: 231 32 459 32 173
Fragments arranged by size:
608 459 296 273 231 180 173 32
32 32 32 32 32
KpnI G_GTAC'C
Cuts at: 0 273 2412
Size: 273 2139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 968 2412
Size: 620 348 1444
Fragments arranged by size:
1444 620 348
MscI TGG'CCA
Cuts at: 0 22 241 487 1529 1606 1848 2344 2412
Size: 22 219 246 1042 77 242 496 68
Fragments arranged by size:
1042 496 246 242 219 77 68 22
MslI CAynn'nnrTG
Cuts at: 0 406 1864 2296 2412
Size: 406 1458 432 116
Fragments arranged by size:
1458 432 406 116
MspA1I CmG'CkG
Cuts at: 0 18 245 711 2013 2412
Size: 18 227 466 1302 399
Fragments arranged by size:
1302 466 399 227 18
NcoI C'CATG_G
Cuts at: 0 1667 2412
Size: 1667 745
NdeI CA'TA_TG
Cuts at: 0 285 2412
Size: 285 2127
NheI G'CTAG_C
Cuts at: 0 1890 2412
Size: 1890 522
NspI r_CATG'y
Cuts at: 0 1056 1164 1695 2412
Size: 1056 108 531 717
Fragments arranged by size:
1056 717 531 108
PasI CC'CwG_GG
Cuts at: 0 1216 2034 2412
Size: 1216 818 378
Fragments arranged by size:
1216 818 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 1602 1713 2412
Size: 1602 111 699
Fragments arranged by size:
1602 699 111
PfoI T'CCnGG_A
Cuts at: 0 137 206 290 665 2359 2412
Size: 137 69 84 375 1694 53
Fragments arranged by size:
1694 375 137 84 69 53
PpuMI rG'GwC_Cy
Cuts at: 0 721 2070 2412
Size: 721 1349 342
Fragments arranged by size:
1349 721 342
PspOMI G'GGCC_C
Cuts at: 0 378 2412
Size: 378 2034
PstI C_TGCA'G
Cuts at: 0 128 1616 1679 2309 2412
Size: 128 1488 63 630 103
Fragments arranged by size:
1488 630 128 103 63
PvuII CAG'CTG
Cuts at: 0 18 711 2412
Size: 18 693 1701
Fragments arranged by size:
1701 693 18
SacI G_AGCT'C
Cuts at: 0 215 1859 2412
Size: 215 1644 553
Fragments arranged by size:
1644 553 215
SapI GCTCTTCn'nnn_
Cuts at: 0 2005 2412
Size: 2005 407
SfcI C'TryA_G
Cuts at: 0 124 474 1612 1675 2305 2412
Size: 124 350 1138 63 630 107
Fragments arranged by size:
1138 630 350 124 107 63
SmaI CCC'GGG
Cuts at: 0 2110 2412
Size: 2110 302
SphI G_CATG'C
Cuts at: 0 1056 2412
Size: 1056 1356
StuI AGG'CCT
Cuts at: 0 995 2275 2412
Size: 995 1280 137
Fragments arranged by size:
1280 995 137
StyI C'CwwG_G
Cuts at: 0 1286 1667 2412
Size: 1286 381 745
Fragments arranged by size:
1286 745 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 396 1269 1324 1329 1822 2412
Size: 54 342 873 55 5 493 590
Fragments arranged by size:
873 590 493 342 55 54 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 805 1752 1798 2091 2412
Size: 805 947 46 293 321
Fragments arranged by size:
947 805 321 293 46
XmaI C'CCGG_G
Cuts at: 0 2108 2412
Size: 2108 304
XmnI GAAnn'nnTTC
Cuts at: 0 1999 2412
Size: 1999 413
ZraI GAC'GTC
Cuts at: 0 2223 2412
Size: 2223 189
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BcgI BclI BglI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NcoI NdeI NheI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SapI SfcI SmaI
SphI StuI StyI TaqII XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AleI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BfrBI BglII BlpI BplI BpuEI
BsaBI BsiWI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I DraI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI MfeI MluI NaeI NarI
NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI