Restriction map for
pF1KA1104
(Linear) (Six-base) MAPSORT of: KIAA1104.seq Check: 1623 from: 1 to: 3111
KIAA1104.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 1318 2816 3111
Size: 1318 1498 295
Fragments arranged by size:
1498 1318 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 2202 2891 3111
Size: 646 1556 689 220
Fragments arranged by size:
1556 689 646 220
AflII C'TTAA_G
Cuts at: 0 748 3111
Size: 748 2363
AflIII A'CryG_T
Cuts at: 0 686 934 3111
Size: 686 248 2177
Fragments arranged by size:
2177 686 248
AgeI A'CCGG_T
Cuts at: 0 383 3111
Size: 383 2728
AleI CACnn'nnGTG
Cuts at: 0 886 3111
Size: 886 2225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 904 2996 3111
Size: 776 128 2092 115
Fragments arranged by size:
2092 776 128 115
AlwNI CAG_nnn'CTG
Cuts at: 0 27 828 1431 3111
Size: 27 801 603 1680
Fragments arranged by size:
1680 801 603 27
ApaI G_GGCC'C
Cuts at: 0 1070 3111
Size: 1070 2041
ApoI r'AATT_y
Cuts at: 0 836 859 922 1417 1447 2138 2677 2693
Size: 836 23 63 495 30 691 539 16
Cuts at: 2693 2836 3111
Size: 143 275
Fragments arranged by size:
836 691 539 495 275 143 63 30
23 16
AvaI C'yCGr_G
Cuts at: 0 172 1822 3067 3111
Size: 172 1650 1245 44
Fragments arranged by size:
1650 1245 172 44
AvrII C'CTAG_G
Cuts at: 0 916 3111
Size: 916 2195
BanI G'GyrC_C
Cuts at: 0 888 2153 3111
Size: 888 1265 958
Fragments arranged by size:
1265 958 888
BanII G_rGCy'C
Cuts at: 0 111 1070 1391 2129 3111
Size: 111 959 321 738 982
Fragments arranged by size:
982 959 738 321 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 251 1033 1040 1930 2396 2566 2868 3111
Size: 251 782 7 890 466 170 302 243
Fragments arranged by size:
890 782 466 302 251 243 170 7
BbvCI CC'TCA_GC
Cuts at: 0 37 1831 2357 3111
Size: 37 1794 526 754
Fragments arranged by size:
1794 754 526 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 446 3111
Size: 446 2665
BfrBI ATG'CAT
Cuts at: 0 2606 3111
Size: 2606 505
BglII A'GATC_T
Cuts at: 0 1607 3111
Size: 1607 1504
BlpI GC'TnA_GC
Cuts at: 0 46 1907 3111
Size: 46 1861 1204
Fragments arranged by size:
1861 1204 46
Bme1580I G_kGCm'C
Cuts at: 0 1070 1847 2156 2468 3111
Size: 1070 777 309 312 643
Fragments arranged by size:
1070 777 643 312 309
BmgBI CAC'GTC
Cuts at: 0 1725 3111
Size: 1725 1386
BmrI ACTGGGnnnn_n'
Cuts at: 0 2829 3041 3111
Size: 2829 212 70
Fragments arranged by size:
2829 212 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1961 2992 3111
Size: 1961 1031 119
Fragments arranged by size:
1961 1031 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1760 1788 2779 3111
Size: 1760 28 991 332
Fragments arranged by size:
1760 991 332 28
Bpu10I CC'TnA_GC
Cuts at: 0 37 1831 2357 3111
Size: 37 1794 526 754
Fragments arranged by size:
1794 754 526 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1549 1637 3111
Size: 337 1212 88 1474
Fragments arranged by size:
1474 1212 337 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 353 705 1198 2206 3111
Size: 344 9 352 493 1008 905
Fragments arranged by size:
1008 905 493 352 344 9
BsaAI yAC'GTr
Cuts at: 0 689 800 1953 2299 2595 3111
Size: 689 111 1153 346 296 516
Fragments arranged by size:
1153 689 516 346 296 111
BsaHI Gr'CG_yC
Cuts at: 0 580 3111
Size: 580 2531
BsaWI w'CCGG_w
Cuts at: 0 383 1002 1927 3111
Size: 383 619 925 1184
Fragments arranged by size:
1184 925 619 383
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 1840 1995 2101 2345 3111
Size: 466 1374 155 106 244 766
Fragments arranged by size:
1374 766 466 244 155 106
BseYI C'CCAG_C
Cuts at: 0 653 1766 1872 2467 3111
Size: 653 1113 106 595 644
Fragments arranged by size:
1113 653 644 595 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 375 3111
Size: 289 86 2736
Fragments arranged by size:
2736 289 86
BsiEI CG_ry'CG
Cuts at: 0 2387 2412 2453 3111
Size: 2387 25 41 658
Fragments arranged by size:
2387 658 41 25
BsiHKAI G_wGCw'C
Cuts at: 0 1391 1958 2093 2129 3043 3111
Size: 1391 567 135 36 914 68
Fragments arranged by size:
1391 914 567 135 68 36
BsiWI C'GTAC_G
Cuts at: 0 800 3111
Size: 800 2311
BsmI GAATG_Cn'
Cuts at: 0 2352 2608 3111
Size: 2352 256 503
Fragments arranged by size:
2352 503 256
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2782 3111
Size: 2782 329
Bsp1286I G_dGCh'C
Cuts at: 0 111 1070 1391 1847 1958 2093 2129 2156
Size: 111 959 321 456 111 135 36 27
Cuts at: 2156 2468 3043 3111
Size: 312 575 68
Fragments arranged by size:
959 575 456 321 312 135 111 111
68 36 27
BspHI T'CATG_A
Cuts at: 0 401 3111
Size: 401 2710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2036 2213 3111
Size: 2036 177 898
Fragments arranged by size:
2036 898 177
BsrBI CCG'CTC
Cuts at: 0 50 3111
Size: 50 3061
BsrDI GCAATG_nn'
Cuts at: 0 789 3111
Size: 789 2322
BsrFI r'CCGG_y
Cuts at: 0 383 3111
Size: 383 2728
BssSI C'ACGA_G
Cuts at: 0 841 1149 1531 3111
Size: 841 308 382 1580
Fragments arranged by size:
1580 841 382 308
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1995 3111
Size: 1995 1116
BstEII G'GTnAC_C
Cuts at: 0 709 3111
Size: 709 2402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 1782 2803 3111
Size: 288 1494 1021 308
Fragments arranged by size:
1494 1021 308 288
BstYI r'GATC_y
Cuts at: 0 134 960 1607 3111
Size: 134 826 647 1504
Fragments arranged by size:
1504 826 647 134
Bsu36I CC'TnA_GG
Cuts at: 0 2284 3111
Size: 2284 827
BtgI C'CryG_G
Cuts at: 0 138 287 884 2499 3111
Size: 138 149 597 1615 612
Fragments arranged by size:
1615 612 597 149 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 93 3111
Size: 93 3018
BtsI GCAGTG_nn'
Cuts at: 0 197 3111
Size: 197 2914
ClaI AT'CG_AT
Cuts at: 0 1507 3111
Size: 1507 1604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 866 901 3111
Size: 866 35 2210
Fragments arranged by size:
2210 866 35
DraI TTT'AAA
Cuts at: 0 594 3111
Size: 594 2517
DrdI GACnn_nn'nnGTC
Cuts at: 0 2796 3111
Size: 2796 315
EaeI y'GGCC_r
Cuts at: 0 236 3005 3053 3111
Size: 236 2769 48 58
Fragments arranged by size:
2769 236 58 48
EarI CTCTTCn'nnn_
Cuts at: 0 1855 2078 2242 3031 3111
Size: 1855 223 164 789 80
Fragments arranged by size:
1855 789 223 164 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 68 3111
Size: 68 3043
EcoICRI GAG'CTC
Cuts at: 0 1389 2127 3111
Size: 1389 738 984
Fragments arranged by size:
1389 984 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1760 1788 2202 2779 2891 3111
Size: 646 1114 28 414 577 112 220
Fragments arranged by size:
1114 646 577 414 220 112 28
EcoO109I rG'GnC_Cy
Cuts at: 0 24 1377 1835 2202 2772 3090 3111
Size: 24 1353 458 367 570 318 21
Fragments arranged by size:
1353 570 458 367 318 24 21
EcoRI G'AATT_C
Cuts at: 0 922 2138 3111
Size: 922 1216 973
Fragments arranged by size:
1216 973 922
EcoRV GAT'ATC
Cuts at: 0 1746 2271 3111
Size: 1746 525 840
Fragments arranged by size:
1746 840 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2328 2413 2583 2884 3111
Size: 2328 85 170 301 227
Fragments arranged by size:
2328 301 227 170 85
HaeII r_GCGC'y
Cuts at: 0 9 3111
Size: 9 3102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 1296 1328 1570 1602 2547 2579
Size: 119 32 1145 32 242 32 945 32
Cuts at: 2579 3111
Size: 532
Fragments arranged by size:
1145 945 532 242 119 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1639 2817 3111
Size: 1639 1178 294
Fragments arranged by size:
1639 1178 294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1700 2392 3111
Size: 1113 587 692 719
Fragments arranged by size:
1113 719 692 587
MscI TGG'CCA
Cuts at: 0 238 3055 3111
Size: 238 2817 56
Fragments arranged by size:
2817 238 56
MslI CAynn'nnrTG
Cuts at: 0 286 400 886 3111
Size: 286 114 486 2225
Fragments arranged by size:
2225 486 286 114
MspA1I CmG'CkG
Cuts at: 0 1738 2222 3111
Size: 1738 484 889
Fragments arranged by size:
1738 889 484
NcoI C'CATG_G
Cuts at: 0 138 287 2499 3111
Size: 138 149 2212 612
Fragments arranged by size:
2212 612 149 138
NsiI A_TGCA'T
Cuts at: 0 2608 3111
Size: 2608 503
NspI r_CATG'y
Cuts at: 0 61 938 3111
Size: 61 877 2173
Fragments arranged by size:
2173 877 61
PasI CC'CwG_GG
Cuts at: 0 905 3111
Size: 905 2206
PciI A'CATG_T
Cuts at: 0 934 3111
Size: 934 2177
PfoI T'CCnGG_A
Cuts at: 0 725 3097 3111
Size: 725 2372 14
Fragments arranged by size:
2372 725 14
PmlI CAC'GTG
Cuts at: 0 1953 3111
Size: 1953 1158
PpuMI rG'GwC_Cy
Cuts at: 0 1377 2202 2772 3090 3111
Size: 1377 825 570 318 21
Fragments arranged by size:
1377 825 570 318 21
PspOMI G'GGCC_C
Cuts at: 0 1066 3111
Size: 1066 2045
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1688 1720 3111
Size: 1688 32 1391
Fragments arranged by size:
1688 1391 32
PstI C_TGCA'G
Cuts at: 0 116 192 1436 1858 2210 3111
Size: 116 76 1244 422 352 901
Fragments arranged by size:
1244 901 422 352 116 76
PvuII CAG'CTG
Cuts at: 0 1738 3111
Size: 1738 1373
SacI G_AGCT'C
Cuts at: 0 1391 2129 3111
Size: 1391 738 982
Fragments arranged by size:
1391 982 738
SalI G'TCGA_C
Cuts at: 0 2815 3111
Size: 2815 296
SanDI GG'GwC_CC
Cuts at: 0 1377 2772 3111
Size: 1377 1395 339
Fragments arranged by size:
1395 1377 339
SapI GCTCTTCn'nnn_
Cuts at: 0 3031 3111
Size: 3031 80
SbfI CC_TGCA'GG
Cuts at: 0 1436 2210 3111
Size: 1436 774 901
Fragments arranged by size:
1436 901 774
ScaI AGT'ACT
Cuts at: 0 297 1759 3111
Size: 297 1462 1352
Fragments arranged by size:
1462 1352 297
SexAI A'CCwGG_T
Cuts at: 0 155 3111
Size: 155 2956
SfcI C'TryA_G
Cuts at: 0 112 188 956 1432 1854 2206 3111
Size: 112 76 768 476 422 352 905
Fragments arranged by size:
905 768 476 422 352 112 76
SmlI C'TyrA_G
Cuts at: 0 316 748 1564 1652 3111
Size: 316 432 816 88 1459
Fragments arranged by size:
1459 816 432 316 88
SspI AAT'ATT
Cuts at: 0 2060 3111
Size: 2060 1051
StuI AGG'CCT
Cuts at: 0 1159 2433 3111
Size: 1159 1274 678
Fragments arranged by size:
1274 1159 678
StyI C'CwwG_G
Cuts at: 0 138 287 458 584 916 1107 1179 2499
Size: 138 149 171 126 332 191 72 1320
Cuts at: 2499 3086 3111
Size: 587 25
Fragments arranged by size:
1320 587 332 191 171 149 138 126
72 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 217 791 2398 2587 3111
Size: 217 574 1607 189 524
Fragments arranged by size:
1607 574 524 217 189
TatI w'GTAC_w
Cuts at: 0 295 1757 3111
Size: 295 1462 1354
Fragments arranged by size:
1462 1354 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 707 739 3111
Size: 438 32 237 32 2372
Fragments arranged by size:
2372 438 237 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1726 3111
Size: 1726 1385
XbaI T'CTAG_A
Cuts at: 0 1623 3111
Size: 1623 1488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 934 1774 1779 2497 3111
Size: 934 840 5 718 614
Fragments arranged by size:
934 840 718 614 5
XmnI GAAnn'nnTTC
Cuts at: 0 409 3111
Size: 409 2702
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AleI AlfI AlwNI
ApaI ApoI AvaI AvrII BanI BanII BbsI BbvCI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PfoI
PmlI PpuMI PspOMI PsrI PstI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AhdI AloI ApaLI
AscI AseI AsiSI BaeI BamHI BbeI BcgI BclI
BglI BmtI BsaBI BsaXI BspEI BsrGI BssHII BstBI
BstZ17I DraIII EagI EcoNI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
XhoI XmaI ZraI