Restriction map for
pF1KA1106
(Linear) (Six-base) MAPSORT of: KIAA1106.seq Check: 5379 from: 1 to: 3396
KIAA1106.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 2300 3313 3396
Size: 2300 1013 83
Fragments arranged by size:
2300 1013 83
Acc65I G'GTAC_C
Cuts at: 0 2801 3396
Size: 2801 595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1124 3157 3396
Size: 308 816 2033 239
Fragments arranged by size:
2033 816 308 239
AflIII A'CryG_T
Cuts at: 0 1616 2744 3396
Size: 1616 1128 652
Fragments arranged by size:
1616 1128 652
AgeI A'CCGG_T
Cuts at: 0 2255 3396
Size: 2255 1141
AhdI GACnn_n'nnGTC
Cuts at: 0 708 3396
Size: 708 2688
AleI CACnn'nnGTG
Cuts at: 0 149 1084 3396
Size: 149 935 2312
Fragments arranged by size:
2312 935 149
AlwNI CAG_nnn'CTG
Cuts at: 0 2085 2106 2195 2982 3219 3396
Size: 2085 21 89 787 237 177
Fragments arranged by size:
2085 787 237 177 89 21
ApoI r'AATT_y
Cuts at: 0 3276 3386 3396
Size: 3276 110 10
Fragments arranged by size:
3276 110 10
AvaI C'yCGr_G
Cuts at: 0 1264 1363 1750 2407 2707 2931 3396
Size: 1264 99 387 657 300 224 465
Fragments arranged by size:
1264 657 465 387 300 224 99
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2425 2458 3396
Size: 2425 33 938
Fragments arranged by size:
2425 938 33
BamHI G'GATC_C
Cuts at: 0 3258 3396
Size: 3258 138
BanI G'GyrC_C
Cuts at: 0 2768 2801 3396
Size: 2768 33 595
Fragments arranged by size:
2768 595 33
BanII G_rGCy'C
Cuts at: 0 1015 1751 2222 3396
Size: 1015 736 471 1174
Fragments arranged by size:
1174 1015 736 471
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 356 551 2369 3145 3396
Size: 296 60 195 1818 776 251
Fragments arranged by size:
1818 776 296 251 195 60
BbvCI CC'TCA_GC
Cuts at: 0 479 2161 3396
Size: 479 1682 1235
Fragments arranged by size:
1682 1235 479
BciVI GTATCCnnnnn_n'
Cuts at: 0 2414 3396
Size: 2414 982
BfrBI ATG'CAT
Cuts at: 0 1467 3396
Size: 1467 1929
BglI GCCn_nnn'nGGC
Cuts at: 0 496 2029 3396
Size: 496 1533 1367
Fragments arranged by size:
1533 1367 496
Bme1580I G_kGCm'C
Cuts at: 0 1489 2554 2773 3396
Size: 1489 1065 219 623
Fragments arranged by size:
1489 1065 623 219
BmgBI CAC'GTC
Cuts at: 0 1617 2889 2942 3396
Size: 1617 1272 53 454
Fragments arranged by size:
1617 1272 454 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 1400 2744 2811 3396
Size: 1400 1344 67 585
Fragments arranged by size:
1400 1344 585 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3233 3396
Size: 3233 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1018 1165 1433 1699 2236 2342 2990
Size: 844 174 147 268 266 537 106 648
Cuts at: 2990 3396
Size: 406
Fragments arranged by size:
844 648 537 406 268 266 174 147
106
Bpu10I CC'TnA_GC
Cuts at: 0 479 1651 1945 2086 2161 2880 3208 3396
Size: 479 1172 294 141 75 719 328 188
Fragments arranged by size:
1172 719 479 328 294 188 141 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1311 1465 2530 3396
Size: 1311 154 1065 866
Fragments arranged by size:
1311 1065 866 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 3396
Size: 865 2531
BsaAI yAC'GTr
Cuts at: 0 1386 1979 3291 3396
Size: 1386 593 1312 105
Fragments arranged by size:
1386 1312 593 105
BsaBI GATnn'nnATC
Cuts at: 0 3329 3396
Size: 3329 67
BsaHI Gr'CG_yC
Cuts at: 0 955 3396
Size: 955 2441
BsaWI w'CCGG_w
Cuts at: 0 1420 1552 2255 2700 3396
Size: 1420 132 703 445 696
Fragments arranged by size:
1420 703 696 445 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1039 1069 1519 1549 2209 2239
Size: 991 30 18 30 450 30 660 30
Cuts at: 2239 3396
Size: 1157
Fragments arranged by size:
1157 991 660 450 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 243 322 337 340 805 817 1015
Size: 31 212 79 15 3 465 12 198
Cuts at: 1015 1230 3166 3178 3396
Size: 215 1936 12 218
Fragments arranged by size:
1936 465 218 215 212 198 79 31
15 12 12 3
BseYI C'CCAG_C
Cuts at: 0 1210 1891 1897 1927 2231 2268 2285 3396
Size: 1210 681 6 30 304 37 17 1111
Fragments arranged by size:
1210 1111 681 304 37 30 17 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1485 2001 3396
Size: 1485 516 1395
Fragments arranged by size:
1485 1395 516
BsiEI CG_ry'CG
Cuts at: 0 1021 1565 3396
Size: 1021 544 1831
Fragments arranged by size:
1831 1021 544
BsiHKAI G_wGCw'C
Cuts at: 0 671 1652 1751 2092 3396
Size: 671 981 99 341 1304
Fragments arranged by size:
1304 981 671 341 99
BsmI GAATG_Cn'
Cuts at: 0 2510 3396
Size: 2510 886
Bsp1286I G_dGCh'C
Cuts at: 0 671 1015 1489 1652 1751 2092 2222 2554
Size: 671 344 474 163 99 341 130 332
Cuts at: 2554 2773 3396
Size: 219 623
Fragments arranged by size:
671 623 474 344 341 332 219 163
130 99
BspEI T'CCGG_A
Cuts at: 0 1420 1552 3396
Size: 1420 132 1844
Fragments arranged by size:
1844 1420 132
BspHI T'CATG_A
Cuts at: 0 1059 3396
Size: 1059 2337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 3396
Size: 219 3177
BsrBI CCG'CTC
Cuts at: 0 1065 3396
Size: 1065 2331
BsrFI r'CCGG_y
Cuts at: 0 1378 2255 2587 3396
Size: 1378 877 332 809
Fragments arranged by size:
1378 877 809 332
BssSI C'ACGA_G
Cuts at: 0 3193 3396
Size: 3193 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1979 2855 3396
Size: 1979 876 541
Fragments arranged by size:
1979 876 541
BstBI TT'CG_AA
Cuts at: 0 2514 3396
Size: 2514 882
BstEII G'GTnAC_C
Cuts at: 0 2415 3396
Size: 2415 981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 1161 1390 1804 2734 3396
Size: 540 621 229 414 930 662
Fragments arranged by size:
930 662 621 540 414 229
BstYI r'GATC_y
Cuts at: 0 3258 3396
Size: 3258 138
BstZ17I GTA'TAC
Cuts at: 0 3314 3396
Size: 3314 82
BtgI C'CryG_G
Cuts at: 0 59 1202 2182 3396
Size: 59 1143 980 1214
Fragments arranged by size:
1214 1143 980 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 13 812 1140 2737 2962 3396
Size: 13 799 328 1597 225 434
Fragments arranged by size:
1597 799 434 328 225 13
BtsI GCAGTG_nn'
Cuts at: 0 120 2249 3396
Size: 120 2129 1147
Fragments arranged by size:
2129 1147 120
DrdI GACnn_nn'nnGTC
Cuts at: 0 1300 3396
Size: 1300 2096
EaeI y'GGCC_r
Cuts at: 0 446 854 928 1199 1380 1583 1739 2033
Size: 446 408 74 271 181 203 156 294
Cuts at: 2033 2525 2718 2780 3396
Size: 492 193 62 616
Fragments arranged by size:
616 492 446 408 294 271 203 193
181 156 74 62
EarI CTCTTCn'nnn_
Cuts at: 0 326 929 1100 3143 3396
Size: 326 603 171 2043 253
Fragments arranged by size:
2043 603 326 253 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2515 3396
Size: 2515 881
EcoICRI GAG'CTC
Cuts at: 0 1749 3396
Size: 1749 1647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 844 1018 1124 1165 1433 1699 2236
Size: 308 536 174 106 41 268 266 537
Cuts at: 2236 2342 2990 3157 3396
Size: 106 648 167 239
Fragments arranged by size:
648 537 536 308 268 266 239 174
167 106 106 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1741 3396
Size: 1741 1655
EcoO109I rG'GnC_Cy
Cuts at: 0 1443 1887 2103 2150 3396
Size: 1443 444 216 47 1246
Fragments arranged by size:
1443 1246 444 216 47
EcoRI G'AATT_C
Cuts at: 0 3386 3396
Size: 3386 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2010 3396
Size: 2010 1386
HaeII r_GCGC'y
Cuts at: 0 1970 2762 3396
Size: 1970 792 634
Fragments arranged by size:
1970 792 634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 694 726 1066 1098 1231 1263
Size: 238 32 424 32 340 32 133 32
Cuts at: 1263 1338 1370 1547 1579 2209 2241 2326 2358
Size: 75 32 177 32 630 32 85 32
Cuts at: 2358 3396
Size: 1038
Fragments arranged by size:
1038 630 424 340 238 177 133 85
75 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1521 3396
Size: 1521 1875
HindIII A'AGCT_T
Cuts at: 0 2962 3396
Size: 2962 434
KpnI G_GTAC'C
Cuts at: 0 2805 3396
Size: 2805 591
MluI A'CGCG_T
Cuts at: 0 2744 3396
Size: 2744 652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1381 1757 3396
Size: 556 825 376 1639
Fragments arranged by size:
1639 825 556 376
MscI TGG'CCA
Cuts at: 0 448 856 1201 1585 1741 2527 3396
Size: 448 408 345 384 156 786 869
Fragments arranged by size:
869 786 448 408 384 345 156
MslI CAynn'nnrTG
Cuts at: 0 125 149 1084 1472 1802 2134 2311 3396
Size: 125 24 935 388 330 332 177 1085
Fragments arranged by size:
1085 935 388 332 330 177 125 24
MspA1I CmG'CkG
Cuts at: 0 89 1037 1677 2184 2192 2268 2894 2997
Size: 89 948 640 507 8 76 626 103
Cuts at: 2997 3246 3396
Size: 249 150
Fragments arranged by size:
948 640 626 507 249 150 103 89
76 8
NcoI C'CATG_G
Cuts at: 0 1202 3396
Size: 1202 2194
NdeI CA'TA_TG
Cuts at: 0 1236 3396
Size: 1236 2160
NsiI A_TGCA'T
Cuts at: 0 1469 3396
Size: 1469 1927
NspI r_CATG'y
Cuts at: 0 711 925 981 1519 2853 3396
Size: 711 214 56 538 1334 543
Fragments arranged by size:
1334 711 543 538 214 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 3198 3231 3396
Size: 3198 33 165
Fragments arranged by size:
3198 165 33
PfoI T'CCnGG_A
Cuts at: 0 2378 2824 3396
Size: 2378 446 572
Fragments arranged by size:
2378 572 446
PmlI CAC'GTG
Cuts at: 0 1979 3396
Size: 1979 1417
PpuMI rG'GwC_Cy
Cuts at: 0 1443 1887 2103 2150 3396
Size: 1443 444 216 47 1246
Fragments arranged by size:
1443 1246 444 216 47
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 56 88 3099 3131 3396
Size: 56 32 3011 32 265
Fragments arranged by size:
3011 265 56 32 32
PstI C_TGCA'G
Cuts at: 0 1572 3396
Size: 1572 1824
PvuI CG_AT'CG
Cuts at: 0 1565 3396
Size: 1565 1831
PvuII CAG'CTG
Cuts at: 0 89 1037 1677 2192 2268 3246 3396
Size: 89 948 640 515 76 978 150
Fragments arranged by size:
978 948 640 515 150 89 76
RsrII CG'GwC_CG
Cuts at: 0 1641 3396
Size: 1641 1755
SacI G_AGCT'C
Cuts at: 0 1751 3396
Size: 1751 1645
SacII CC_GC'GG
Cuts at: 0 2185 3396
Size: 2185 1211
SanDI GG'GwC_CC
Cuts at: 0 1443 2150 3396
Size: 1443 707 1246
Fragments arranged by size:
1443 1246 707
SfcI C'TryA_G
Cuts at: 0 1568 1698 3396
Size: 1568 130 1698
Fragments arranged by size:
1698 1568 130
SmaI CCC'GGG
Cuts at: 0 1266 1365 2409 2709 3396
Size: 1266 99 1044 300 687
Fragments arranged by size:
1266 1044 687 300 99
SmlI C'TyrA_G
Cuts at: 0 1326 1480 1750 2545 3396
Size: 1326 154 270 795 851
Fragments arranged by size:
1326 851 795 270 154
SspI AAT'ATT
Cuts at: 0 1759 3396
Size: 1759 1637
StuI AGG'CCT
Cuts at: 0 1636 3396
Size: 1636 1760
StyI C'CwwG_G
Cuts at: 0 167 651 1124 1202 1586 1742 1826 2774
Size: 167 484 473 78 384 156 84 948
Cuts at: 2774 3396
Size: 622
Fragments arranged by size:
948 622 484 473 384 167 156 84
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1043 2823 3396
Size: 1043 1780 573
Fragments arranged by size:
1780 1043 573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 852 884 1039 1071 3396
Size: 852 32 155 32 2325
Fragments arranged by size:
2325 852 155 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 507 3396
Size: 507 2889
XbaI T'CTAG_A
Cuts at: 0 2388 3396
Size: 2388 1008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3078 3396
Size: 3078 318
XhoI C'TCGA_G
Cuts at: 0 1750 3396
Size: 1750 1646
XmaI C'CCGG_G
Cuts at: 0 1264 1363 2407 2707 3396
Size: 1264 99 1044 300 689
Fragments arranged by size:
1264 1044 689 300 99
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AhdI AleI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KpnI MluI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI
PmlI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SacII SanDI SfcI SmaI SmlI SspI StuI StyI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BbeI BcgI BclI
BglII BlpI BmtI BsiWI BsmBI BsrDI BsrGI BssHII
Bsu36I ClaI CspCI DraI DraIII EagI EcoRV FseI
FspI FspAI HpaI KasI MfeI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TatI XmnI ZraI