Restriction map for
pF1KA1109
(Linear) (Six-base) MAPSORT of: KIAA1109.seq Check: 858 from: 1 to: 9048
KIAA1109.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1285 9048
Size: 1285 7763
AatII G_ACGT'C
Cuts at: 0 6870 9048
Size: 6870 2178
AccI GT'mk_AC
Cuts at: 0 6420 7807 9048
Size: 6420 1387 1241
Fragments arranged by size:
6420 1387 1241
AclI AA'CG_TT
Cuts at: 0 4746 7274 9048
Size: 4746 2528 1774
Fragments arranged by size:
4746 2528 1774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2111 2195 2455 2704 3785 4148 4592 4976
Size: 2111 84 260 249 1081 363 444 384
Cuts at: 4976 5192 5923 6593 7130 7556 7566 7588 8129
Size: 216 731 670 537 426 10 22 541
Cuts at: 8129 8563 9048
Size: 434 485
Fragments arranged by size:
2111 1081 731 670 541 537 485 444
434 426 384 363 260 249 216 84
22 10
AflII C'TTAA_G
Cuts at: 0 2461 4915 7597 9048
Size: 2461 2454 2682 1451
Fragments arranged by size:
2682 2461 2454 1451
AflIII A'CryG_T
Cuts at: 0 2257 9048
Size: 2257 6791
AhdI GACnn_n'nnGTC
Cuts at: 0 3691 5055 5196 9048
Size: 3691 1364 141 3852
Fragments arranged by size:
3852 3691 1364 141
AleI CACnn'nnGTG
Cuts at: 0 76 8389 8591 9048
Size: 76 8313 202 457
Fragments arranged by size:
8313 457 202 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5620 9048
Size: 5620 3428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6666 6698 9048
Size: 6666 32 2350
Fragments arranged by size:
6666 2350 32
AlwNI CAG_nnn'CTG
Cuts at: 0 537 4342 4603 6616 9048
Size: 537 3805 261 2013 2432
Fragments arranged by size:
3805 2432 2013 537 261
ApaI G_GGCC'C
Cuts at: 0 8070 9048
Size: 8070 978
ApaLI G'TGCA_C
Cuts at: 0 1620 3071 9048
Size: 1620 1451 5977
Fragments arranged by size:
5977 1620 1451
ApoI r'AATT_y
Cuts at: 0 47 215 634 1998 2920 2968 3485 4055
Size: 47 168 419 1364 922 48 517 570
Cuts at: 4055 4256 4503 4739 6196 6821 6894 7511 7765
Size: 201 247 236 1457 625 73 617 254
Cuts at: 7765 8046 8397 9048
Size: 281 351 651
Fragments arranged by size:
1457 1364 922 651 625 617 570 517
419 351 281 254 247 236 201 168
73 48 47
AseI AT'TA_AT
Cuts at: 0 1570 8836 9048
Size: 1570 7266 212
Fragments arranged by size:
7266 1570 212
AvaI C'yCGr_G
Cuts at: 0 4940 9048
Size: 4940 4108
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4188 4221 9048
Size: 4188 33 4827
Fragments arranged by size:
4827 4188 33
BamHI G'GATC_C
Cuts at: 0 1382 2441 4413 8952 9048
Size: 1382 1059 1972 4539 96
Fragments arranged by size:
4539 1972 1382 1059 96
BanI G'GyrC_C
Cuts at: 0 4300 6691 9048
Size: 4300 2391 2357
Fragments arranged by size:
4300 2391 2357
BanII G_rGCy'C
Cuts at: 0 2245 4964 6605 7561 7803 8070 8615 9048
Size: 2245 2719 1641 956 242 267 545 433
Fragments arranged by size:
2719 2245 1641 956 545 433 267 242
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 1156 2201 2692 4484 4596 4911 5192
Size: 595 561 1045 491 1792 112 315 281
Cuts at: 5192 5246 6116 9048
Size: 54 870 2932
Fragments arranged by size:
2932 1792 1045 870 595 561 491 315
281 112 54
BbvCI CC'TCA_GC
Cuts at: 0 1203 2342 2738 2927 9048
Size: 1203 1139 396 189 6121
Fragments arranged by size:
6121 1203 1139 396 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 1389 4000 4216 5016 6735 7576 9048
Size: 1389 2611 216 800 1719 841 1472
Fragments arranged by size:
2611 1719 1472 1389 841 800 216
BclI T'GATC_A
Cuts at: 0 8142 9048
Size: 8142 906
BfrBI ATG'CAT
Cuts at: 0 97 236 6230 6491 9048
Size: 97 139 5994 261 2557
Fragments arranged by size:
5994 2557 261 139 97
BglI GCCn_nnn'nGGC
Cuts at: 0 6410 9048
Size: 6410 2638
BglII A'GATC_T
Cuts at: 0 82 2011 7734 9048
Size: 82 1929 5723 1314
Fragments arranged by size:
5723 1929 1314 82
BlpI GC'TnA_GC
Cuts at: 0 530 8609 9048
Size: 530 8079 439
Fragments arranged by size:
8079 530 439
Bme1580I G_kGCm'C
Cuts at: 0 1624 1647 2673 3075 8070 9048
Size: 1624 23 1026 402 4995 978
Fragments arranged by size:
4995 1624 1026 978 402 23
BmgBI CAC'GTC
Cuts at: 0 868 9048
Size: 868 8180
BmrI ACTGGGnnnn_n'
Cuts at: 0 7496 9048
Size: 7496 1552
BmtI G_CTAG'C
Cuts at: 0 4610 6368 9048
Size: 4610 1758 2680
Fragments arranged by size:
4610 2680 1758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 836 2596 4015 4118 5120 5709 6304
Size: 749 87 1760 1419 103 1002 589 595
Cuts at: 6304 6347 7744 7934 8121 8808 9048
Size: 43 1397 190 187 687 240
Fragments arranged by size:
1760 1419 1397 1002 749 687 595 589
240 190 187 103 87 43
Bpu10I CC'TnA_GC
Cuts at: 0 1203 2342 2738 2927 9048
Size: 1203 1139 396 189 6121
Fragments arranged by size:
6121 1203 1139 396 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 231 1573 6495 6667 7545 9048
Size: 157 74 1342 4922 172 878 1503
Fragments arranged by size:
4922 1503 1342 878 172 157 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 4094 6875 9048
Size: 4094 2781 2173
Fragments arranged by size:
4094 2781 2173
BsaBI GATnn'nnATC
Cuts at: 0 3671 3731 9048
Size: 3671 60 5317
Fragments arranged by size:
5317 3671 60
BsaHI Gr'CG_yC
Cuts at: 0 6867 8259 9048
Size: 6867 1392 789
Fragments arranged by size:
6867 1392 789
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 834 864 3141 3171 4539 4569 4803 4833
Size: 834 30 2277 30 1368 30 234 30
Cuts at: 4833 5657 5687 5784 5814 7167 7197 7415 7445
Size: 824 30 97 30 1353 30 218 30
Cuts at: 7445 9048
Size: 1603
Fragments arranged by size:
2277 1603 1368 1353 834 824 234 218
97 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 956 3481 5050 5789 5798 5915 6290 6613
Size: 956 2525 1569 739 9 117 375 323
Cuts at: 6613 7439 8438 8957 9048
Size: 826 999 519 91
Fragments arranged by size:
2525 1569 999 956 826 739 519 375
323 117 91 9
BseYI C'CCAG_C
Cuts at: 0 871 5858 7110 9048
Size: 871 4987 1252 1938
Fragments arranged by size:
4987 1938 1252 871
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 928 4064 8984 9048
Size: 355 573 3136 4920 64
Fragments arranged by size:
4920 3136 573 355 64
BsiHKAI G_wGCw'C
Cuts at: 0 1624 3075 4964 7803 8615 9048
Size: 1624 1451 1889 2839 812 433
Fragments arranged by size:
2839 1889 1624 1451 812 433
BsmI GAATG_Cn'
Cuts at: 0 797 4284 5156 6232 7985 9048
Size: 797 3487 872 1076 1753 1063
Fragments arranged by size:
3487 1753 1076 1063 872 797
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5294 9048
Size: 5294 3754
Bsp1286I G_dGCh'C
Cuts at: 0 1624 1647 2245 2673 3075 4964 6605 7561
Size: 1624 23 598 428 402 1889 1641 956
Cuts at: 7561 7803 8070 8615 9048
Size: 242 267 545 433
Fragments arranged by size:
1889 1641 1624 956 598 545 433 428
402 267 242 23
BspHI T'CATG_A
Cuts at: 0 1656 3383 8628 9048
Size: 1656 1727 5245 420
Fragments arranged by size:
5245 1727 1656 420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1285 9048
Size: 1285 7763
BsrDI GCAATG_nn'
Cuts at: 0 4490 5944 9048
Size: 4490 1454 3104
Fragments arranged by size:
4490 3104 1454
BsrFI r'CCGG_y
Cuts at: 0 695 5593 9048
Size: 695 4898 3455
Fragments arranged by size:
4898 3455 695
BsrGI T'GTAC_A
Cuts at: 0 267 3369 8160 9048
Size: 267 3102 4791 888
Fragments arranged by size:
4791 3102 888 267
BssSI C'ACGA_G
Cuts at: 0 7541 9048
Size: 7541 1507
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4603 9048
Size: 4603 4445
BstBI TT'CG_AA
Cuts at: 0 2772 9048
Size: 2772 6276
BstEII G'GTnAC_C
Cuts at: 0 8199 9048
Size: 8199 849
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4015 6842 9048
Size: 4015 2827 2206
Fragments arranged by size:
4015 2827 2206
BstYI r'GATC_y
Cuts at: 0 82 1382 1831 2011 2441 4413 5731 6087
Size: 82 1300 449 180 430 1972 1318 356
Cuts at: 6087 7734 8952 9048
Size: 1647 1218 96
Fragments arranged by size:
1972 1647 1318 1300 1218 449 430 356
180 96 82
BtgI C'CryG_G
Cuts at: 0 2445 7228 7677 8280 9048
Size: 2445 4783 449 603 768
Fragments arranged by size:
4783 2445 768 603 449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 428 9048
Size: 428 8620
BtsI GCAGTG_nn'
Cuts at: 0 125 475 836 984 1689 4151 6482 9048
Size: 125 350 361 148 705 2462 2331 2566
Fragments arranged by size:
2566 2462 2331 705 361 350 148 125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 579 614 1085 1120 1158 1193 2039 2074
Size: 579 35 471 35 38 35 846 35
Cuts at: 2074 8193 8228 9048
Size: 6119 35 820
Fragments arranged by size:
6119 846 820 579 471 38 35 35
35 35 35
DraI TTT'AAA
Cuts at: 0 185 504 3671 3750 6795 8490 9048
Size: 185 319 3167 79 3045 1695 558
Fragments arranged by size:
3167 3045 1695 558 319 185 79
DrdI GACnn_nn'nnGTC
Cuts at: 0 1160 8711 9048
Size: 1160 7551 337
Fragments arranged by size:
7551 1160 337
EaeI y'GGCC_r
Cuts at: 0 3533 5344 6411 9048
Size: 3533 1811 1067 2637
Fragments arranged by size:
3533 2637 1811 1067
EarI CTCTTCn'nnn_
Cuts at: 0 1370 2431 4167 4782 4952 5168 6147 7152
Size: 1370 1061 1736 615 170 216 979 1005
Cuts at: 7152 7327 8330 9048
Size: 175 1003 718
Fragments arranged by size:
1736 1370 1061 1005 1003 979 718 615
216 175 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7823 9048
Size: 7823 1225
EcoICRI GAG'CTC
Cuts at: 0 4962 7801 8613 9048
Size: 4962 2839 812 435
Fragments arranged by size:
4962 2839 812 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 836 2111 2195 2455 2596 2704 3785
Size: 749 87 1275 84 260 141 108 1081
Cuts at: 3785 4015 4118 4148 4592 4976 5120 5192 5709
Size: 230 103 30 444 384 144 72 517
Cuts at: 5709 5923 6304 6347 6593 7130 7556 7566 7588
Size: 214 381 43 246 537 426 10 22
Cuts at: 7588 7744 7934 8121 8129 8563 8808 9048
Size: 156 190 187 8 434 245 240
Fragments arranged by size:
1275 1081 749 537 517 444 434 426
384 381 260 246 245 240 230 214
190 187 156 144 141 108 103 87
84 72 43 30 22 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6572 9048
Size: 6572 2476
EcoO109I rG'GnC_Cy
Cuts at: 0 7223 8067 9048
Size: 7223 844 981
Fragments arranged by size:
7223 981 844
EcoRI G'AATT_C
Cuts at: 0 1998 8397 9048
Size: 1998 6399 651
Fragments arranged by size:
6399 1998 651
EcoRV GAT'ATC
Cuts at: 0 4659 7715 9048
Size: 4659 3056 1333
Fragments arranged by size:
4659 3056 1333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 595 2141 3835 6992 9048
Size: 595 1546 1694 3157 2056
Fragments arranged by size:
3157 2056 1694 1546 595
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1024 1054 1056 1086 1248 1280 2292 2324
Size: 1024 30 2 30 162 32 1012 32
Cuts at: 2324 3677 3709 3773 3805 4537 4569 4972 5004
Size: 1353 32 64 32 732 32 403 32
Cuts at: 5004 5041 5073 5182 5214 5490 5511 5522 5543
Size: 37 32 109 32 276 21 11 21
Cuts at: 5543 6288 6304 6320 6336 6766 6798 7455 7487
Size: 745 16 16 16 430 32 657 32
Cuts at: 7487 8097 8129 8476 8508 8623 8655 8725 8757
Size: 610 32 347 32 115 32 70 32
Cuts at: 8757 9048
Size: 291
Fragments arranged by size:
1353 1024 1012 745 732 657 610 430
403 347 291 276 162 115 109 70
64 37 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 21 21 16 16 16 11
2
HincII GTy'rAC
Cuts at: 0 35 3149 6421 7724 9048
Size: 35 3114 3272 1303 1324
Fragments arranged by size:
3272 3114 1324 1303 35
HindIII A'AGCT_T
Cuts at: 0 930 3614 6974 7600 9048
Size: 930 2684 3360 626 1448
Fragments arranged by size:
3360 2684 1448 930 626
MfeI C'AATT_G
Cuts at: 0 3839 9048
Size: 3839 5209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1216 2212 7413 9048
Size: 843 373 996 5201 1635
Fragments arranged by size:
5201 1635 996 843 373
MscI TGG'CCA
Cuts at: 0 5346 9048
Size: 5346 3702
MslI CAynn'nnrTG
Cuts at: 0 76 92 566 572 1033 2084 2525 3142
Size: 76 16 474 6 461 1051 441 617
Cuts at: 3142 6524 7024 8028 8389 8591 8593 9048
Size: 3382 500 1004 361 202 2 455
Fragments arranged by size:
3382 1051 1004 617 500 474 461 455
441 361 202 76 16 6 2
MspA1I CmG'CkG
Cuts at: 0 111 139 2131 4342 6043 9048
Size: 111 28 1992 2211 1701 3005
Fragments arranged by size:
3005 2211 1992 1701 111 28
NcoI C'CATG_G
Cuts at: 0 2445 7228 7677 8280 9048
Size: 2445 4783 449 603 768
Fragments arranged by size:
4783 2445 768 603 449
NdeI CA'TA_TG
Cuts at: 0 2081 3953 6525 7058 8018 9048
Size: 2081 1872 2572 533 960 1030
Fragments arranged by size:
2572 2081 1872 1030 960 533
NheI G'CTAG_C
Cuts at: 0 4606 6364 9048
Size: 4606 1758 2684
Fragments arranged by size:
4606 2684 1758
NsiI A_TGCA'T
Cuts at: 0 99 238 6232 6493 9048
Size: 99 139 5994 261 2555
Fragments arranged by size:
5994 2555 261 139 99
NspI r_CATG'y
Cuts at: 0 97 862 1732 2261 2350 4160 6234 6491
Size: 97 765 870 529 89 1810 2074 257
Cuts at: 6491 6495 7029 7386 8033 9048
Size: 4 534 357 647 1015
Fragments arranged by size:
2074 1810 1015 870 765 647 534 529
357 257 97 89 4
PasI CC'CwG_GG
Cuts at: 0 6913 9048
Size: 6913 2135
PciI A'CATG_T
Cuts at: 0 2257 9048
Size: 2257 6791
PflMI CCAn_nnn'nTGG
Cuts at: 0 142 6907 7975 8281 9048
Size: 142 6765 1068 306 767
Fragments arranged by size:
6765 1068 767 306 142
PfoI T'CCnGG_A
Cuts at: 0 953 4992 5018 5136 5515 5661 7760 9048
Size: 953 4039 26 118 379 146 2099 1288
Fragments arranged by size:
4039 2099 1288 953 379 146 118 26
PpuMI rG'GwC_Cy
Cuts at: 0 7223 9048
Size: 7223 1825
PsiI TTA'TAA
Cuts at: 0 1679 3896 9048
Size: 1679 2217 5152
Fragments arranged by size:
5152 2217 1679
PspOMI G'GGCC_C
Cuts at: 0 8066 9048
Size: 8066 982
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1002 1034 5399 5431 9048
Size: 1002 32 4365 32 3617
Fragments arranged by size:
4365 3617 1002 32 32
PstI C_TGCA'G
Cuts at: 0 5880 6312 9048
Size: 5880 432 2736
Fragments arranged by size:
5880 2736 432
PvuII CAG'CTG
Cuts at: 0 111 139 2131 4342 6043 9048
Size: 111 28 1992 2211 1701 3005
Fragments arranged by size:
3005 2211 1992 1701 111 28
SacI G_AGCT'C
Cuts at: 0 4964 7803 8615 9048
Size: 4964 2839 812 433
Fragments arranged by size:
4964 2839 812 433
SalI G'TCGA_C
Cuts at: 0 6419 9048
Size: 6419 2629
SanDI GG'GwC_CC
Cuts at: 0 7223 9048
Size: 7223 1825
SapI GCTCTTCn'nnn_
Cuts at: 0 4167 4952 7327 8330 9048
Size: 4167 785 2375 1003 718
Fragments arranged by size:
4167 2375 1003 785 718
ScaI AGT'ACT
Cuts at: 0 4143 9048
Size: 4143 4905
SexAI A'CCwGG_T
Cuts at: 0 705 3984 6381 9048
Size: 705 3279 2397 2667
Fragments arranged by size:
3279 2667 2397 705
SfcI C'TryA_G
Cuts at: 0 2246 2627 2664 2825 4325 5384 5617 5876
Size: 2246 381 37 161 1500 1059 233 259
Cuts at: 5876 6152 6308 9004 9048
Size: 276 156 2696 44
Fragments arranged by size:
2696 2246 1500 1059 381 276 259 233
161 156 44 37
SmlI C'TyrA_G
Cuts at: 0 172 210 1552 2461 4915 4940 6474 6646
Size: 172 38 1342 909 2454 25 1534 172
Cuts at: 6646 7560 7597 9048
Size: 914 37 1451
Fragments arranged by size:
2454 1534 1451 1342 914 909 172 172
38 37 25
SpeI A'CTAG_T
Cuts at: 0 6114 7321 9048
Size: 6114 1207 1727
Fragments arranged by size:
6114 1727 1207
SphI G_CATG'C
Cuts at: 0 4160 6491 9048
Size: 4160 2331 2557
Fragments arranged by size:
4160 2557 2331
SspI AAT'ATT
Cuts at: 0 2198 2538 5397 8094 9048
Size: 2198 340 2859 2697 954
Fragments arranged by size:
2859 2697 2198 954 340
StuI AGG'CCT
Cuts at: 0 4012 9048
Size: 4012 5036
StyI C'CwwG_G
Cuts at: 0 895 1314 2445 6876 7228 7677 8280 8470
Size: 895 419 1131 4431 352 449 603 190
Cuts at: 8470 9048
Size: 578
Fragments arranged by size:
4431 1131 895 603 578 449 419 352
190
SwaI ATTT'AAAT
Cuts at: 0 3671 9048
Size: 3671 5377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 818 1197 5468 5596 7036 9048
Size: 818 379 4271 128 1440 2012
Fragments arranged by size:
4271 2012 1440 818 379 128
TatI w'GTAC_w
Cuts at: 0 267 1421 2602 3369 4141 5445 8160 9048
Size: 267 1154 1181 767 772 1304 2715 888
Fragments arranged by size:
2715 1304 1181 1154 888 772 767 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 834 866 3226 3258 4377 4409 5835 5867
Size: 834 32 2360 32 1119 32 1426 32
Cuts at: 5867 7308 7340 9048
Size: 1441 32 1708
Fragments arranged by size:
2360 1708 1441 1426 1119 834 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 1906 8817 9048
Size: 1906 6911 231
Fragments arranged by size:
6911 1906 231
XhoI C'TCGA_G
Cuts at: 0 4940 9048
Size: 4940 4108
XmnI GAAnn'nnTTC
Cuts at: 0 3379 6627 7161 9048
Size: 3379 3248 534 1887
Fragments arranged by size:
3379 3248 1887 534
ZraI GAC'GTC
Cuts at: 0 6868 9048
Size: 6868 2180
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NheI NsiI NspI PasI
PciI PflMI PfoI PpuMI PsiI PspOMI PsrI PstI
PvuII SacI SalI SanDI SapI ScaI SexAI SfcI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AscI AsiSI AvrII BbeI BcgI
BplI BsaAI BsaWI BsiEI BsiWI BspEI BsrBI BssHII
BstZ17I Bsu36I ClaI DraIII EagI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI NaeI NarI NgoMIV
NotI NruI PacI PmeI PmlI PpiI PshAI PspXI
PvuI RsrII SacII SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI Tth111I XcmI XmaI