Restriction map for
pF1KA1111
(Linear) (Six-base) MAPSORT of: KIAA1111.seq Check: 7630 from: 1 to: 3180
KIAA1111.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2196 3180
Size: 2196 984
AccI GT'mk_AC
Cuts at: 0 1895 3180
Size: 1895 1285
Acc65I G'GTAC_C
Cuts at: 0 875 3180
Size: 875 2305
AclI AA'CG_TT
Cuts at: 0 1448 3180
Size: 1448 1732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 453 511 1167 1517 1612 1726 2827
Size: 439 14 58 656 350 95 114 1101
Cuts at: 2827 3044 3180
Size: 217 136
Fragments arranged by size:
1101 656 439 350 217 136 114 95
58 14
AflIII A'CryG_T
Cuts at: 0 33 179 3180
Size: 33 146 3001
Fragments arranged by size:
3001 146 33
AleI CACnn'nnGTG
Cuts at: 0 1534 3180
Size: 1534 1646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 532 3180
Size: 532 2648
AlwNI CAG_nnn'CTG
Cuts at: 0 1704 2737 3180
Size: 1704 1033 443
Fragments arranged by size:
1704 1033 443
ApaI G_GGCC'C
Cuts at: 0 284 3180
Size: 284 2896
ApoI r'AATT_y
Cuts at: 0 448 688 1936 2017 2616 3180
Size: 448 240 1248 81 599 564
Fragments arranged by size:
1248 599 564 448 240 81
AvaI C'yCGr_G
Cuts at: 0 2699 3180
Size: 2699 481
BamHI G'GATC_C
Cuts at: 0 1058 3180
Size: 1058 2122
BanI G'GyrC_C
Cuts at: 0 117 862 875 1747 3053 3180
Size: 117 745 13 872 1306 127
Fragments arranged by size:
1306 872 745 127 117 13
BanII G_rGCy'C
Cuts at: 0 284 361 380 1810 2380 3180
Size: 284 77 19 1430 570 800
Fragments arranged by size:
1430 800 570 284 77 19
BbeI G_GCGC'C
Cuts at: 0 3057 3180
Size: 3057 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 1436 1462 1714 1937 3030 3180
Size: 355 1081 26 252 223 1093 150
Fragments arranged by size:
1093 1081 355 252 223 150 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 3154 3180
Size: 3154 26
BglII A'GATC_T
Cuts at: 0 899 1401 3180
Size: 899 502 1779
Fragments arranged by size:
1779 899 502
BlpI GC'TnA_GC
Cuts at: 0 1165 3180
Size: 1165 2015
Bme1580I G_kGCm'C
Cuts at: 0 284 479 2398 3180
Size: 284 195 1919 782
Fragments arranged by size:
1919 782 284 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 2360 3180
Size: 2360 820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 972 2364 2484 2584 3180
Size: 76 896 1392 120 100 596
Fragments arranged by size:
1392 896 596 120 100 76
Bpu10I CC'TnA_GC
Cuts at: 0 2921 3180
Size: 2921 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 2176 2816 3180
Size: 871 1305 640 364
Fragments arranged by size:
1305 871 640 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 783 1195 3180
Size: 783 412 1985
Fragments arranged by size:
1985 783 412
BsaHI Gr'CG_yC
Cuts at: 0 1075 3054 3180
Size: 1075 1979 126
Fragments arranged by size:
1979 1075 126
BsaWI w'CCGG_w
Cuts at: 0 380 3180
Size: 380 2800
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1261 1291 3180
Size: 1261 30 1889
Fragments arranged by size:
1889 1261 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 2116 2494 2497 2500 2503 2889 2892
Size: 238 1878 378 3 3 3 386 3
Cuts at: 2892 2906 3180
Size: 14 274
Fragments arranged by size:
1878 386 378 274 238 14 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 1491 2244 2254 2391 2420 2750 3047 3180
Size: 1491 753 10 137 29 330 297 133
Fragments arranged by size:
1491 753 330 297 137 133 29 10
BsiEI CG_ry'CG
Cuts at: 0 81 3141 3180
Size: 81 3060 39
Fragments arranged by size:
3060 81 39
BsiHKAI G_wGCw'C
Cuts at: 0 380 1171 2380 3180
Size: 380 791 1209 800
Fragments arranged by size:
1209 800 791 380
BsmI GAATG_Cn'
Cuts at: 0 2177 3180
Size: 2177 1003
Bsp1286I G_dGCh'C
Cuts at: 0 284 361 380 479 1171 1810 2380 2398
Size: 284 77 19 99 692 639 570 18
Cuts at: 2398 3180
Size: 782
Fragments arranged by size:
782 692 639 570 284 99 77 19
18
BspEI T'CCGG_A
Cuts at: 0 380 3180
Size: 380 2800
BspHI T'CATG_A
Cuts at: 0 164 815 3180
Size: 164 651 2365
Fragments arranged by size:
2365 651 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2196 2306 3180
Size: 2196 110 874
Fragments arranged by size:
2196 874 110
BsrBI CCG'CTC
Cuts at: 0 2974 3180
Size: 2974 206
BsrFI r'CCGG_y
Cuts at: 0 76 120 137 1495 2319 3180
Size: 76 44 17 1358 824 861
Fragments arranged by size:
1358 861 824 76 44 17
BsrGI T'GTAC_A
Cuts at: 0 798 3180
Size: 798 2382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2285 2737 3180
Size: 2285 452 443
Fragments arranged by size:
2285 452 443
BstBI TT'CG_AA
Cuts at: 0 738 3180
Size: 738 2442
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2462 3024 3163 3180
Size: 2462 562 139 17
Fragments arranged by size:
2462 562 139 17
BstYI r'GATC_y
Cuts at: 0 307 899 1058 1364 1401 1415 1484 3180
Size: 307 592 159 306 37 14 69 1696
Fragments arranged by size:
1696 592 307 306 159 69 37 14
BtgI C'CryG_G
Cuts at: 0 303 443 1290 2632 3180
Size: 303 140 847 1342 548
Fragments arranged by size:
1342 847 548 303 140
BtsI GCAGTG_nn'
Cuts at: 0 252 1555 2376 2391 3180
Size: 252 1303 821 15 789
Fragments arranged by size:
1303 821 789 252 15
DraIII CAC_nnn'GTG
Cuts at: 0 1055 3180
Size: 1055 2125
EaeI y'GGCC_r
Cuts at: 0 74 78 766 1406 1659 2291 2452 3116
Size: 74 4 688 640 253 632 161 664
Cuts at: 3116 3138 3180
Size: 22 42
Fragments arranged by size:
688 664 640 632 253 161 74 42
22 4
EagI C'GGCC_G
Cuts at: 0 78 3138 3180
Size: 78 3060 42
Fragments arranged by size:
3060 78 42
EarI CTCTTCn'nnn_
Cuts at: 0 215 649 1186 1612 1846 3180
Size: 215 434 537 426 234 1334
Fragments arranged by size:
1334 537 434 426 234 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1408 3180
Size: 904 504 1772
Fragments arranged by size:
1772 904 504
EcoICRI GAG'CTC
Cuts at: 0 378 2378 3180
Size: 378 2000 802
Fragments arranged by size:
2000 802 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 439 453 511 972 1167 1517 1612
Size: 76 363 14 58 461 195 350 95
Cuts at: 1612 1726 2364 2484 2584 2827 3044 3180
Size: 114 638 120 100 243 217 136
Fragments arranged by size:
638 461 363 350 243 217 195 136
120 114 100 95 76 58 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 1329 2236 3180
Size: 493 836 907 944
Fragments arranged by size:
944 907 836 493
EcoO109I rG'GnC_Cy
Cuts at: 0 281 1651 2215 2241 2696 2937 3111 3180
Size: 281 1370 564 26 455 241 174 69
Fragments arranged by size:
1370 564 455 281 241 174 69 26
EcoRI G'AATT_C
Cuts at: 0 448 688 2616 3180
Size: 448 240 1928 564
Fragments arranged by size:
1928 564 448 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1623 2167 3180
Size: 1623 544 1013
Fragments arranged by size:
1623 1013 544
FseI GG_CCGG'CC
Cuts at: 0 80 3180
Size: 80 3100
HaeII r_GCGC'y
Cuts at: 0 2171 3057 3180
Size: 2171 886 123
Fragments arranged by size:
2171 886 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 1691 1759 1791 2646 2678 2926 2958
Size: 1659 32 68 32 855 32 248 32
Cuts at: 2958 3180
Size: 222
Fragments arranged by size:
1659 855 248 222 68 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 619 2149 3180
Size: 619 1530 1031
Fragments arranged by size:
1530 1031 619
KasI G'GCGC_C
Cuts at: 0 3053 3180
Size: 3053 127
KpnI G_GTAC'C
Cuts at: 0 879 3180
Size: 879 2301
MluI A'CGCG_T
Cuts at: 0 33 3180
Size: 33 3147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1464 3180
Size: 1464 1716
MscI TGG'CCA
Cuts at: 0 768 1408 1661 2293 3118 3180
Size: 768 640 253 632 825 62
Fragments arranged by size:
825 768 640 632 253 62
MslI CAynn'nnrTG
Cuts at: 0 820 1129 1358 1534 3022 3161 3180
Size: 820 309 229 176 1488 139 19
Fragments arranged by size:
1488 820 309 229 176 139 19
MspA1I CmG'CkG
Cuts at: 0 650 2737 3051 3180
Size: 650 2087 314 129
Fragments arranged by size:
2087 650 314 129
NaeI GCC'GGC
Cuts at: 0 78 139 1497 3180
Size: 78 61 1358 1683
Fragments arranged by size:
1683 1358 78 61
NarI GG'CG_CC
Cuts at: 0 3054 3180
Size: 3054 126
NcoI C'CATG_G
Cuts at: 0 1290 2632 3180
Size: 1290 1342 548
Fragments arranged by size:
1342 1290 548
NgoMIV G'CCGG_C
Cuts at: 0 76 137 1495 3180
Size: 76 61 1358 1685
Fragments arranged by size:
1685 1358 76 61
NspI r_CATG'y
Cuts at: 0 183 2283 3180
Size: 183 2100 897
Fragments arranged by size:
2100 897 183
PasI CC'CwG_GG
Cuts at: 0 2431 3180
Size: 2431 749
PciI A'CATG_T
Cuts at: 0 179 3180
Size: 179 3001
PflMI CCAn_nnn'nTGG
Cuts at: 0 1055 1211 3180
Size: 1055 156 1969
Fragments arranged by size:
1969 1055 156
PfoI T'CCnGG_A
Cuts at: 0 452 1232 1367 3180
Size: 452 780 135 1813
Fragments arranged by size:
1813 780 452 135
PpuMI rG'GwC_Cy
Cuts at: 0 2215 2696 3180
Size: 2215 481 484
Fragments arranged by size:
2215 484 481
PspOMI G'GGCC_C
Cuts at: 0 280 3180
Size: 280 2900
PstI C_TGCA'G
Cuts at: 0 1481 1967 2303 2339 2733 3180
Size: 1481 486 336 36 394 447
Fragments arranged by size:
1481 486 447 394 336 36
PvuII CAG'CTG
Cuts at: 0 2737 3180
Size: 2737 443
SacI G_AGCT'C
Cuts at: 0 380 2380 3180
Size: 380 2000 800
Fragments arranged by size:
2000 800 380
SanDI GG'GwC_CC
Cuts at: 0 2696 3180
Size: 2696 484
SbfI CC_TGCA'GG
Cuts at: 0 2339 3180
Size: 2339 841
ScaI AGT'ACT
Cuts at: 0 808 3180
Size: 808 2372
SexAI A'CCwGG_T
Cuts at: 0 992 1283 3180
Size: 992 291 1897
Fragments arranged by size:
1897 992 291
SfcI C'TryA_G
Cuts at: 0 1477 1963 2299 2335 2729 2761 3180
Size: 1477 486 336 36 394 32 419
Fragments arranged by size:
1477 486 419 394 336 36 32
SfoI GGC'GCC
Cuts at: 0 3055 3180
Size: 3055 125
SmaI CCC'GGG
Cuts at: 0 2701 3180
Size: 2701 479
SmlI C'TyrA_G
Cuts at: 0 886 2191 2831 3180
Size: 886 1305 640 349
Fragments arranged by size:
1305 886 640 349
SphI G_CATG'C
Cuts at: 0 2283 3180
Size: 2283 897
StuI AGG'CCT
Cuts at: 0 1589 2341 3180
Size: 1589 752 839
Fragments arranged by size:
1589 839 752
StyI C'CwwG_G
Cuts at: 0 1290 1598 1646 2632 3180
Size: 1290 308 48 986 548
Fragments arranged by size:
1290 986 548 308 48
TatI w'GTAC_w
Cuts at: 0 798 806 882 2966 3180
Size: 798 8 76 2084 214
Fragments arranged by size:
2084 798 214 76 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 2148 2180 2961 2993 3180
Size: 1261 32 855 32 781 32 187
Fragments arranged by size:
1261 855 781 187 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1447 2205 2388 3111 3180
Size: 1447 758 183 723 69
Fragments arranged by size:
1447 758 723 183 69
XmaI C'CCGG_G
Cuts at: 0 2699 3180
Size: 2699 481
XmnI GAAnn'nnTTC
Cuts at: 0 413 3180
Size: 413 2767
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BciVI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BstAPI BstBI BstXI BstYI BtgI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI HaeII Hin4I HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SanDI SbfI ScaI SexAI SfcI
SfoI SmaI SmlI SphI StuI StyI TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI
BglI BmgBI BmtI BplI BsaAI BsaBI BsgI BsiWI
BsmBI BsrDI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EcoRV FspI FspAI HincII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI
ZraI