Restriction map for
pF1KSDA1112
(Linear) (Six-base) MAPSORT of: KIAA1112.seq Check: 3424 from: 1 to: 2010
KIAA1112.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 10 914 1760 1809 2010
Size: 10 904 846 49 201
Fragments arranged by size:
904 846 201 49 10
AatII G_ACGT'C
Cuts at: 0 659 2010
Size: 659 1351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 2010
Size: 617 1393
AfeI AGC'GCT
Cuts at: 0 1786 2010
Size: 1786 224
AflIII A'CryG_T
Cuts at: 0 956 1193 1616 2010
Size: 956 237 423 394
Fragments arranged by size:
956 423 394 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 2010
Size: 445 1565
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 2010
Size: 1033 32 945
Fragments arranged by size:
1033 945 32
AlwNI CAG_nnn'CTG
Cuts at: 0 136 424 636 2010
Size: 136 288 212 1374
Fragments arranged by size:
1374 288 212 136
ApaI G_GGCC'C
Cuts at: 0 114 2010
Size: 114 1896
ApaLI G'TGCA_C
Cuts at: 0 280 1758 2010
Size: 280 1478 252
Fragments arranged by size:
1478 280 252
AvaI C'yCGr_G
Cuts at: 0 1506 1520 1771 2010
Size: 1506 14 251 239
Fragments arranged by size:
1506 251 239 14
AvrII C'CTAG_G
Cuts at: 0 163 2010
Size: 163 1847
BanI G'GyrC_C
Cuts at: 0 1084 1732 2010
Size: 1084 648 278
Fragments arranged by size:
1084 648 278
BanII G_rGCy'C
Cuts at: 0 114 444 569 770 1166 1450 1492 2010
Size: 114 330 125 201 396 284 42 518
Fragments arranged by size:
518 396 330 284 201 125 114 42
BbeI G_GCGC'C
Cuts at: 0 1736 2010
Size: 1736 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 223 2010
Size: 223 1787
BbvCI CC'TCA_GC
Cuts at: 0 748 877 2010
Size: 748 129 1133
Fragments arranged by size:
1133 748 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1066 1100 1414 1448 2010
Size: 1066 34 314 34 562
Fragments arranged by size:
1066 562 314 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 790 2010
Size: 790 1220
BglII A'GATC_T
Cuts at: 0 1118 1277 1473 2010
Size: 1118 159 196 537
Fragments arranged by size:
1118 537 196 159
BlpI GC'TnA_GC
Cuts at: 0 1183 2010
Size: 1183 827
Bme1580I G_kGCm'C
Cuts at: 0 114 284 1762 2010
Size: 114 170 1478 248
Fragments arranged by size:
1478 248 170 114
BmgBI CAC'GTC
Cuts at: 0 252 1617 2010
Size: 252 1365 393
Fragments arranged by size:
1365 393 252
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 1470 2010
Size: 26 1444 540
Fragments arranged by size:
1444 540 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1492 2010
Size: 1492 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 311 324 495 554 1060 1117 1675
Size: 232 79 13 171 59 506 57 558
Cuts at: 1675 1690 2010
Size: 15 320
Fragments arranged by size:
558 506 320 232 171 79 59 57
15 13
Bpu10I CC'TnA_GC
Cuts at: 0 748 877 1072 2010
Size: 748 129 195 938
Fragments arranged by size:
938 748 195 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1520 1777 2010
Size: 1435 85 257 233
Fragments arranged by size:
1435 257 233 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 1682 2010
Size: 1682 328
BsaAI yAC'GTr
Cuts at: 0 1194 2010
Size: 1194 816
BsaBI GATnn'nnATC
Cuts at: 0 1237 2010
Size: 1237 773
BsaHI Gr'CG_yC
Cuts at: 0 656 782 1733 2010
Size: 656 126 951 277
Fragments arranged by size:
951 656 277 126
BsaWI w'CCGG_w
Cuts at: 0 1266 2010
Size: 1266 744
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1033 1063 2010
Size: 475 30 528 30 947
Fragments arranged by size:
947 528 475 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 496 982 1934 2010
Size: 106 390 486 952 76
Fragments arranged by size:
952 486 390 106 76
BseYI C'CCAG_C
Cuts at: 0 636 649 1080 1328 1385 2010
Size: 636 13 431 248 57 625
Fragments arranged by size:
636 625 431 248 57 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 487 1291 1708 1843 2010
Size: 250 237 804 417 135 167
Fragments arranged by size:
804 417 250 237 167 135
BsiHKAI G_wGCw'C
Cuts at: 0 284 378 413 444 569 1166 1450 1492
Size: 284 94 35 31 125 597 284 42
Cuts at: 1492 1762 2010
Size: 270 248
Fragments arranged by size:
597 284 284 270 248 125 94 42
35 31
BsmI GAATG_Cn'
Cuts at: 0 1890 2010
Size: 1890 120
Bsp1286I G_dGCh'C
Cuts at: 0 114 284 378 413 444 569 770 1166
Size: 114 170 94 35 31 125 201 396
Cuts at: 1166 1450 1492 1762 2010
Size: 284 42 270 248
Fragments arranged by size:
396 284 270 248 201 170 125 114
94 42 35 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 509 914 1610 1760 1809 1890 2010
Size: 10 499 405 696 150 49 81 120
Fragments arranged by size:
696 499 405 150 120 81 49 10
BsrBI CCG'CTC
Cuts at: 0 182 808 888 1401 2010
Size: 182 626 80 513 609
Fragments arranged by size:
626 609 513 182 80
BsrFI r'CCGG_y
Cuts at: 0 1214 1634 2010
Size: 1214 420 376
Fragments arranged by size:
1214 420 376
BssHII G'CGCG_C
Cuts at: 0 948 2010
Size: 948 1062
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1878 2010
Size: 1878 132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 2010
Size: 99 1911
BstYI r'GATC_y
Cuts at: 0 872 1118 1277 1473 2010
Size: 872 246 159 196 537
Fragments arranged by size:
872 537 246 196 159
BtgI C'CryG_G
Cuts at: 0 620 770 1728 2010
Size: 620 150 958 282
Fragments arranged by size:
958 620 282 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 590 2010
Size: 590 1420
BtsI GCAGTG_nn'
Cuts at: 0 284 422 588 943 2010
Size: 284 138 166 355 1067
Fragments arranged by size:
1067 355 284 166 138
DrdI GACnn_nn'nnGTC
Cuts at: 0 454 2010
Size: 454 1556
EaeI y'GGCC_r
Cuts at: 0 403 791 1301 2010
Size: 403 388 510 709
Fragments arranged by size:
709 510 403 388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 838 1597 1941 2010
Size: 838 759 344 69
Fragments arranged by size:
838 759 344 69
EcoICRI GAG'CTC
Cuts at: 0 442 567 1164 1448 1490 2010
Size: 442 125 597 284 42 520
Fragments arranged by size:
597 520 442 284 125 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 311 324 495 554 617 1060 1117
Size: 232 79 13 171 59 63 443 57
Cuts at: 1117 1675 1690 2010
Size: 558 15 320
Fragments arranged by size:
558 443 320 232 171 79 63 59
57 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1803 2010
Size: 1803 207
EcoO109I rG'GnC_Cy
Cuts at: 0 111 486 1035 1599 2010
Size: 111 375 549 564 411
Fragments arranged by size:
564 549 411 375 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 1731 2010
Size: 102 1629 279
Fragments arranged by size:
1629 279 102
HaeII r_GCGC'y
Cuts at: 0 1065 1736 1788 2010
Size: 1065 671 52 222
Fragments arranged by size:
1065 671 222 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 741 773 2010
Size: 741 32 1237
Fragments arranged by size:
1237 741 32
KasI G'GCGC_C
Cuts at: 0 1732 2010
Size: 1732 278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2010
Size: 403 1607
MscI TGG'CCA
Cuts at: 0 405 2010
Size: 405 1605
MslI CAynn'nnrTG
Cuts at: 0 97 287 427 664 2010
Size: 97 190 140 237 1346
Fragments arranged by size:
1346 237 190 140 97
MspA1I CmG'CkG
Cuts at: 0 454 552 772 803 975 1013 1299 1827
Size: 454 98 220 31 172 38 286 528
Cuts at: 1827 2010
Size: 183
Fragments arranged by size:
528 454 286 220 183 172 98 38
31
NaeI GCC'GGC
Cuts at: 0 1216 1636 2010
Size: 1216 420 374
Fragments arranged by size:
1216 420 374
NarI GG'CG_CC
Cuts at: 0 1733 2010
Size: 1733 277
NcoI C'CATG_G
Cuts at: 0 620 2010
Size: 620 1390
NgoMIV G'CCGG_C
Cuts at: 0 1214 1634 2010
Size: 1214 420 376
Fragments arranged by size:
1214 420 376
NspI r_CATG'y
Cuts at: 0 960 1369 2010
Size: 960 409 641
Fragments arranged by size:
960 641 409
PasI CC'CwG_GG
Cuts at: 0 5 2010
Size: 5 2005
PciI A'CATG_T
Cuts at: 0 956 2010
Size: 956 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 2010
Size: 636 1374
PfoI T'CCnGG_A
Cuts at: 0 1094 2010
Size: 1094 916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 2010
Size: 1033 32 945
Fragments arranged by size:
1033 945 32
PpuMI rG'GwC_Cy
Cuts at: 0 486 1599 2010
Size: 486 1113 411
Fragments arranged by size:
1113 486 411
PshAI GACnn'nnGTC
Cuts at: 0 859 2010
Size: 859 1151
PspOMI G'GGCC_C
Cuts at: 0 110 2010
Size: 110 1900
PspXI vC'TCGA_Gb
Cuts at: 0 1520 2010
Size: 1520 490
PstI C_TGCA'G
Cuts at: 0 132 1226 1298 1865 2010
Size: 132 1094 72 567 145
Fragments arranged by size:
1094 567 145 132 72
PvuII CAG'CTG
Cuts at: 0 454 552 975 1299 1827 2010
Size: 454 98 423 324 528 183
Fragments arranged by size:
528 454 423 324 183 98
SacI G_AGCT'C
Cuts at: 0 444 569 1166 1450 1492 2010
Size: 444 125 597 284 42 518
Fragments arranged by size:
597 518 444 284 125 42
SacII CC_GC'GG
Cuts at: 0 773 2010
Size: 773 1237
ScaI AGT'ACT
Cuts at: 0 1132 2010
Size: 1132 878
SfcI C'TryA_G
Cuts at: 0 128 522 1222 1294 1861 2010
Size: 128 394 700 72 567 149
Fragments arranged by size:
700 567 394 149 128 72
SfoI GGC'GCC
Cuts at: 0 1734 2010
Size: 1734 276
SmlI C'TyrA_G
Cuts at: 0 1414 1520 1535 1792 2010
Size: 1414 106 15 257 218
Fragments arranged by size:
1414 257 218 106 15
SphI G_CATG'C
Cuts at: 0 1369 2010
Size: 1369 641
StuI AGG'CCT
Cuts at: 0 1024 1210 1798 2010
Size: 1024 186 588 212
Fragments arranged by size:
1024 588 212 186
StyI C'CwwG_G
Cuts at: 0 163 620 2010
Size: 163 457 1390
Fragments arranged by size:
1390 457 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1072 2010
Size: 1072 938
TatI w'GTAC_w
Cuts at: 0 1130 1196 1622 2010
Size: 1130 66 426 388
Fragments arranged by size:
1130 426 388 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 2010
Size: 190 32 1788
Fragments arranged by size:
1788 190 32
Tth111I GACn'n_nGTC
Cuts at: 0 613 2010
Size: 613 1397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1377 2010
Size: 1155 222 633
Fragments arranged by size:
1155 633 222
XhoI C'TCGA_G
Cuts at: 0 1520 2010
Size: 1520 490
XmnI GAAnn'nnTTC
Cuts at: 0 55 1024 1714 2010
Size: 55 969 690 296
Fragments arranged by size:
969 690 296 55
ZraI GAC'GTC
Cuts at: 0 657 2010
Size: 657 1353
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlfI AloI AlwNI
ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII ScaI SfcI SfoI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI ApoI
AscI AseI AsiSI BaeI BamHI BciVI BclI BfrBI
BmtI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII EagI EarI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PsiI PsrI
PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
XbaI XmaI