Restriction map for
pF1KA1113
(Linear) (Six-base) MAPSORT of: KIAA1113.seq Check: 2063 from: 1 to: 3381
KIAA1113.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 379 1212 3381
Size: 379 833 2169
Fragments arranged by size:
2169 833 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 2363 3013 3381
Size: 629 1734 650 368
Fragments arranged by size:
1734 650 629 368
AflIII A'CryG_T
Cuts at: 0 718 868 883 3381
Size: 718 150 15 2498
Fragments arranged by size:
2498 718 150 15
AleI CACnn'nnGTG
Cuts at: 0 118 2215 3381
Size: 118 2097 1166
Fragments arranged by size:
2097 1166 118
AlwNI CAG_nnn'CTG
Cuts at: 0 793 1450 1624 3381
Size: 793 657 174 1757
Fragments arranged by size:
1757 793 657 174
ApaI G_GGCC'C
Cuts at: 0 86 185 3381
Size: 86 99 3196
Fragments arranged by size:
3196 99 86
ApoI r'AATT_y
Cuts at: 0 745 2941 3381
Size: 745 2196 440
Fragments arranged by size:
2196 745 440
AseI AT'TA_AT
Cuts at: 0 1118 3161 3381
Size: 1118 2043 220
Fragments arranged by size:
2043 1118 220
AvaI C'yCGr_G
Cuts at: 0 213 463 495 3381
Size: 213 250 32 2886
Fragments arranged by size:
2886 250 213 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 907 940 3381
Size: 907 33 2441
Fragments arranged by size:
2441 907 33
BanI G'GyrC_C
Cuts at: 0 539 3381
Size: 539 2842
BanII G_rGCy'C
Cuts at: 0 86 185 220 419 1933 2561 3381
Size: 86 99 35 199 1514 628 820
Fragments arranged by size:
1514 820 628 199 99 86 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 427 668 2293 2666 2896 3326 3381
Size: 427 241 1625 373 230 430 55
Fragments arranged by size:
1625 430 427 373 241 230 55
BbvCI CC'TCA_GC
Cuts at: 0 164 3381
Size: 164 3217
BciVI GTATCCnnnnn_n'
Cuts at: 0 1796 3381
Size: 1796 1585
BclI T'GATC_A
Cuts at: 0 764 1706 2310 3381
Size: 764 942 604 1071
Fragments arranged by size:
1071 942 764 604
BfrBI ATG'CAT
Cuts at: 0 2488 3381
Size: 2488 893
BglII A'GATC_T
Cuts at: 0 2691 3236 3381
Size: 2691 545 145
Fragments arranged by size:
2691 545 145
BlpI GC'TnA_GC
Cuts at: 0 481 2560 3381
Size: 481 2079 821
Fragments arranged by size:
2079 821 481
Bme1580I G_kGCm'C
Cuts at: 0 86 185 259 491 544 3381
Size: 86 99 74 232 53 2837
Fragments arranged by size:
2837 232 99 86 74 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 3381
Size: 538 2843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1793 2710 3258 3381
Size: 1793 917 548 123
Fragments arranged by size:
1793 917 548 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 281 284 1472 1531 2144 3381
Size: 278 3 3 1188 59 613 1237
Fragments arranged by size:
1237 1188 613 278 59 3 3
Bpu10I CC'TnA_GC
Cuts at: 0 164 3188 3381
Size: 164 3024 193
Fragments arranged by size:
3024 193 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 2061 3381
Size: 1012 1049 1320
Fragments arranged by size:
1320 1049 1012
BsaI GGTCTCn'nnnn_
Cuts at: 0 2214 3357 3381
Size: 2214 1143 24
Fragments arranged by size:
2214 1143 24
BsaHI Gr'CG_yC
Cuts at: 0 286 3381
Size: 286 3095
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 276 306 3381
Size: 276 30 3075
Fragments arranged by size:
3075 276 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 148 151 154 201 1688 2418 2639
Size: 145 3 3 3 47 1487 730 221
Cuts at: 2639 2702 3381
Size: 63 679
Fragments arranged by size:
1487 730 679 221 145 63 47 3
3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1051 3381
Size: 695 356 2330
Fragments arranged by size:
2330 695 356
BsiEI CG_ry'CG
Cuts at: 0 179 3381
Size: 179 3202
BsmI GAATG_Cn'
Cuts at: 0 565 1788 3381
Size: 565 1223 1593
Fragments arranged by size:
1593 1223 565
Bsp1286I G_dGCh'C
Cuts at: 0 86 185 220 259 419 491 544 1933
Size: 86 99 35 39 160 72 53 1389
Cuts at: 1933 2561 3381
Size: 628 820
Fragments arranged by size:
1389 820 628 160 99 86 72 53
39 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 379 1212 2471 2532 3381
Size: 379 833 1259 61 849
Fragments arranged by size:
1259 849 833 379 61
BsrBI CCG'CTC
Cuts at: 0 35 316 3381
Size: 35 281 3065
Fragments arranged by size:
3065 281 35
BsrFI r'CCGG_y
Cuts at: 0 60 157 288 401 3381
Size: 60 97 131 113 2980
Fragments arranged by size:
2980 131 113 97 60
BsrGI T'GTAC_A
Cuts at: 0 831 3381
Size: 831 2550
BssHII G'CGCG_C
Cuts at: 0 56 3381
Size: 56 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 393 1722 3381
Size: 393 1329 1659
Fragments arranged by size:
1659 1329 393
BstBI TT'CG_AA
Cuts at: 0 2592 3381
Size: 2592 789
BstXI CCAn_nnnn'nTGG
Cuts at: 0 526 2061 3381
Size: 526 1535 1320
Fragments arranged by size:
1535 1320 526
BstYI r'GATC_y
Cuts at: 0 2691 3236 3381
Size: 2691 545 145
Fragments arranged by size:
2691 545 145
BtgI C'CryG_G
Cuts at: 0 116 203 2879 3381
Size: 116 87 2676 502
Fragments arranged by size:
2676 502 116 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2018 3381
Size: 2018 1363
DraIII CAC_nnn'GTG
Cuts at: 0 1339 3381
Size: 1339 2042
EaeI y'GGCC_r
Cuts at: 0 176 200 1091 3074 3381
Size: 176 24 891 1983 307
Fragments arranged by size:
1983 891 307 176 24
EagI C'GGCC_G
Cuts at: 0 176 3381
Size: 176 3205
EarI CTCTTCn'nnn_
Cuts at: 0 3278 3381
Size: 3278 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 115 427 3381
Size: 19 96 312 2954
Fragments arranged by size:
2954 312 96 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 281 284 629 1472 1531 2144 2363
Size: 278 3 3 345 843 59 613 219
Cuts at: 2363 3013 3381
Size: 650 368
Fragments arranged by size:
843 650 613 368 345 278 219 59
3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2159 3381
Size: 2159 1222
EcoO109I rG'GnC_Cy
Cuts at: 0 322 1966 2164 3251 3381
Size: 322 1644 198 1087 130
Fragments arranged by size:
1644 1087 322 198 130
EcoRI G'AATT_C
Cuts at: 0 2941 3381
Size: 2941 440
FspI TGC'GCA
Cuts at: 0 2864 3381
Size: 2864 517
HaeII r_GCGC'y
Cuts at: 0 164 477 3381
Size: 164 313 2904
Fragments arranged by size:
2904 313 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 619 651 2059 2091 2433 2465
Size: 276 32 311 32 1408 32 342 32
Cuts at: 2465 3381
Size: 916
Fragments arranged by size:
1408 916 342 311 276 32 32 32
32
HincII GTy'rAC
Cuts at: 0 811 3381
Size: 811 2570
HindIII A'AGCT_T
Cuts at: 0 941 3025 3381
Size: 941 2084 356
Fragments arranged by size:
2084 941 356
MfeI C'AATT_G
Cuts at: 0 965 1256 3381
Size: 965 291 2125
Fragments arranged by size:
2125 965 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 2042 2770 3381
Size: 777 1265 728 611
Fragments arranged by size:
1265 777 728 611
MscI TGG'CCA
Cuts at: 0 1093 3381
Size: 1093 2288
MslI CAynn'nnrTG
Cuts at: 0 118 2215 3381
Size: 118 2097 1166
Fragments arranged by size:
2097 1166 118
MspA1I CmG'CkG
Cuts at: 0 50 118 205 1817 2258 3381
Size: 50 68 87 1612 441 1123
Fragments arranged by size:
1612 1123 441 87 68 50
NaeI GCC'GGC
Cuts at: 0 159 290 403 3381
Size: 159 131 113 2978
Fragments arranged by size:
2978 159 131 113
NgoMIV G'CCGG_C
Cuts at: 0 157 288 401 3381
Size: 157 131 113 2980
Fragments arranged by size:
2980 157 131 113
NsiI A_TGCA'T
Cuts at: 0 2490 3381
Size: 2490 891
NspI r_CATG'y
Cuts at: 0 722 872 887 1234 1557 1879 2418 3381
Size: 722 150 15 347 323 322 539 963
Fragments arranged by size:
963 722 539 347 323 322 150 15
PasI CC'CwG_GG
Cuts at: 0 1870 3381
Size: 1870 1511
PciI A'CATG_T
Cuts at: 0 718 868 883 3381
Size: 718 150 15 2498
Fragments arranged by size:
2498 718 150 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1394 1450 3381
Size: 1394 56 1931
Fragments arranged by size:
1931 1394 56
PfoI T'CCnGG_A
Cuts at: 0 342 362 2181 2945 3059 3381
Size: 342 20 1819 764 114 322
Fragments arranged by size:
1819 764 342 322 114 20
PpuMI rG'GwC_Cy
Cuts at: 0 322 1966 2164 3251 3381
Size: 322 1644 198 1087 130
Fragments arranged by size:
1644 1087 322 198 130
PsiI TTA'TAA
Cuts at: 0 2989 3381
Size: 2989 392
PspOMI G'GGCC_C
Cuts at: 0 82 181 3381
Size: 82 99 3200
Fragments arranged by size:
3200 99 82
PstI C_TGCA'G
Cuts at: 0 2529 3381
Size: 2529 852
PvuII CAG'CTG
Cuts at: 0 2258 3381
Size: 2258 1123
SacII CC_GC'GG
Cuts at: 0 119 206 3381
Size: 119 87 3175
Fragments arranged by size:
3175 119 87
SanDI GG'GwC_CC
Cuts at: 0 322 3381
Size: 322 3059
ScaI AGT'ACT
Cuts at: 0 2232 2241 3381
Size: 2232 9 1140
Fragments arranged by size:
2232 1140 9
SfcI C'TryA_G
Cuts at: 0 1186 1937 2525 3381
Size: 1186 751 588 856
Fragments arranged by size:
1186 856 751 588
SmaI CCC'GGG
Cuts at: 0 497 3381
Size: 497 2884
SmlI C'TyrA_G
Cuts at: 0 991 2076 3381
Size: 991 1085 1305
Fragments arranged by size:
1305 1085 991
SpeI A'CTAG_T
Cuts at: 0 652 3381
Size: 652 2729
SphI G_CATG'C
Cuts at: 0 1879 3381
Size: 1879 1502
SspI AAT'ATT
Cuts at: 0 1828 3381
Size: 1828 1553
StuI AGG'CCT
Cuts at: 0 1211 3381
Size: 1211 2170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2067 3381
Size: 2067 1314
TatI w'GTAC_w
Cuts at: 0 649 831 2230 2239 3212 3369 3381
Size: 649 182 1399 9 973 157 12
Fragments arranged by size:
1399 973 649 182 157 12 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2136 2168 3381
Size: 2136 32 1213
Fragments arranged by size:
2136 1213 32
XbaI T'CTAG_A
Cuts at: 0 2469 3381
Size: 2469 912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2660 2771 3381
Size: 2660 111 610
Fragments arranged by size:
2660 610 111
XmaI C'CCGG_G
Cuts at: 0 495 3381
Size: 495 2886
XmnI GAAnn'nnTTC
Cuts at: 0 856 944 2027 2903 3381
Size: 856 88 1083 876 478
Fragments arranged by size:
1083 876 856 478 88
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApoI AseI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiEI BsmI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstXI BstYI BtgI BtgZI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PpuMI
PsiI PspOMI PstI PvuII SacII SanDI ScaI SfcI
SmaI SmlI SpeI SphI SspI StuI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AsiSI AvrII BamHI BbeI
BcgI BglI BmgBI BmtI BsaAI BsaBI BsaWI BseYI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrDI BssSI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI
EcoRV FalI FseI FspAI HpaI KasI KpnI MluI
NarI NcoI NdeI NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SrfI StyI SwaI Tth111I XhoI ZraI