(Linear) (Six-base) MAPSORT of: KIAA1121.seq Check: 1427 from: 1 to: 4059 KIAA1121.orf With 182 enzymes: SgfI * March 2, 2010 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2924 4059 Size: 2924 1135 AatII G_ACGT'C Cuts at: 0 942 4059 Size: 942 3117 AccI GT'mk_AC Cuts at: 0 942 2412 2792 4059 Size: 942 1470 380 1267 Fragments arranged by size: 1470 1267 942 380 AclI AA'CG_TT Cuts at: 0 2331 4059 Size: 2331 1728 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 1048 2438 2662 3205 3272 3307 4059 Size: 583 465 1390 224 543 67 35 752 Fragments arranged by size: 1390 752 583 543 465 224 67 35 AflIII A'CryG_T Cuts at: 0 1010 1655 2709 4059 Size: 1010 645 1054 1350 Fragments arranged by size: 1350 1054 1010 645 AgeI A'CCGG_T Cuts at: 0 473 4059 Size: 473 3586 AhdI GACnn_n'nnGTC Cuts at: 0 12 4059 Size: 12 4047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 82 4059 Size: 82 3977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 851 883 1150 1182 4059 Size: 851 32 267 32 2877 Fragments arranged by size: 2877 851 267 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 456 2859 4059 Size: 456 2403 1200 Fragments arranged by size: 2403 1200 456 ApoI r'AATT_y Cuts at: 0 718 976 1999 2902 4059 Size: 718 258 1023 903 1157 Fragments arranged by size: 1157 1023 903 718 258 AvaI C'yCGr_G Cuts at: 0 620 3673 4059 Size: 620 3053 386 Fragments arranged by size: 3053 620 386 BanI G'GyrC_C Cuts at: 0 154 160 1330 2221 3138 3184 4059 Size: 154 6 1170 891 917 46 875 Fragments arranged by size: 1170 917 891 875 154 46 6 BanII G_rGCy'C Cuts at: 0 124 706 1980 4059 Size: 124 582 1274 2079 Fragments arranged by size: 2079 1274 582 124 BbeI G_GCGC'C Cuts at: 0 158 164 2225 4059 Size: 158 6 2061 1834 Fragments arranged by size: 2061 1834 158 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 621 4049 4058 4059 Size: 621 3428 9 1 Fragments arranged by size: 3428 621 9 1 BbvCI CC'TCA_GC Cuts at: 0 682 1937 4059 Size: 682 1255 2122 Fragments arranged by size: 2122 1255 682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2165 2199 4059 Size: 2165 34 1860 Fragments arranged by size: 2165 1860 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1002 1086 4059 Size: 1002 84 2973 Fragments arranged by size: 2973 1002 84 BclI T'GATC_A Cuts at: 0 1299 3774 4059 Size: 1299 2475 285 Fragments arranged by size: 2475 1299 285 BglI GCCn_nnn'nGGC Cuts at: 0 251 4059 Size: 251 3808 BglII A'GATC_T Cuts at: 0 1518 3195 4059 Size: 1518 1677 864 Fragments arranged by size: 1677 1518 864 BlpI GC'TnA_GC Cuts at: 0 700 4059 Size: 700 3359 Bme1580I G_kGCm'C Cuts at: 0 1333 1892 4059 Size: 1333 559 2167 Fragments arranged by size: 2167 1333 559 BmgBI CAC'GTC Cuts at: 0 3684 4059 Size: 3684 375 BmrI ACTGGGnnnn_n' Cuts at: 0 2476 4059 Size: 2476 1583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 252 1084 2807 4059 Size: 252 832 1723 1252 Fragments arranged by size: 1723 1252 832 252 Bpu10I CC'TnA_GC Cuts at: 0 682 1937 4059 Size: 682 1255 2122 Fragments arranged by size: 2122 1255 682 BsaAI yAC'GTr Cuts at: 0 3699 4059 Size: 3699 360 BsaHI Gr'CG_yC Cuts at: 0 155 161 939 1403 2222 3215 3692 4059 Size: 155 6 778 464 819 993 477 367 Fragments arranged by size: 993 819 778 477 464 367 155 6 BsaWI w'CCGG_w Cuts at: 0 473 3824 3953 4059 Size: 473 3351 129 106 Fragments arranged by size: 3351 473 129 106 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 158 188 851 881 1015 1045 1150 1180 Size: 158 30 663 30 134 30 105 30 Cuts at: 1180 1787 1817 1916 1946 3775 3805 4059 Size: 607 30 99 30 1829 30 254 Fragments arranged by size: 1829 663 607 254 158 134 105 99 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 64 346 349 352 1036 1162 1295 Size: 61 3 282 3 3 684 126 133 Cuts at: 1295 2509 2704 3763 4059 Size: 1214 195 1059 296 Fragments arranged by size: 1214 1059 684 296 282 195 133 126 61 3 3 3 BseYI C'CCAG_C Cuts at: 0 103 141 238 274 1745 4059 Size: 103 38 97 36 1471 2314 Fragments arranged by size: 2314 1471 103 97 38 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 559 1660 1689 3226 3792 4059 Size: 200 359 1101 29 1537 566 267 Fragments arranged by size: 1537 1101 566 359 267 200 29 BsiEI CG_ry'CG Cuts at: 0 128 261 573 4059 Size: 128 133 312 3486 Fragments arranged by size: 3486 312 133 128 BsiHKAI G_wGCw'C Cuts at: 0 71 706 1399 1980 2696 4059 Size: 71 635 693 581 716 1363 Fragments arranged by size: 1363 716 693 635 581 71 BsmI GAATG_Cn' Cuts at: 0 1569 4059 Size: 1569 2490 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2761 3967 4059 Size: 2761 1206 92 Fragments arranged by size: 2761 1206 92 Bsp1286I G_dGCh'C Cuts at: 0 71 124 706 1333 1399 1892 1980 2696 Size: 71 53 582 627 66 493 88 716 Cuts at: 2696 4059 Size: 1363 Fragments arranged by size: 1363 716 627 582 493 88 71 66 53 BspEI T'CCGG_A Cuts at: 0 3953 4059 Size: 3953 106 BspHI T'CATG_A Cuts at: 0 527 4059 Size: 527 3532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1052 1632 2924 3248 4059 Size: 1052 580 1292 324 811 Fragments arranged by size: 1292 1052 811 580 324 BsrBI CCG'CTC Cuts at: 0 56 318 3003 4059 Size: 56 262 2685 1056 Fragments arranged by size: 2685 1056 262 56 BsrDI GCAATG_nn' Cuts at: 0 2901 4059 Size: 2901 1158 BsrFI r'CCGG_y Cuts at: 0 127 157 260 428 473 1071 2609 4059 Size: 127 30 103 168 45 598 1538 1450 Fragments arranged by size: 1538 1450 598 168 127 103 45 30 BsrGI T'GTAC_A Cuts at: 0 1013 3755 4059 Size: 1013 2742 304 Fragments arranged by size: 2742 1013 304 BssHII G'CGCG_C Cuts at: 0 89 91 4059 Size: 89 2 3968 Fragments arranged by size: 3968 89 2 BstBI TT'CG_AA Cuts at: 0 857 4059 Size: 857 3202 BstXI CCAn_nnnn'nTGG Cuts at: 0 420 1002 2281 2831 3657 4059 Size: 420 582 1279 550 826 402 Fragments arranged by size: 1279 826 582 550 420 402 BstYI r'GATC_y Cuts at: 0 1518 2955 3195 4059 Size: 1518 1437 240 864 Fragments arranged by size: 1518 1437 864 240 Bsu36I CC'TnA_GG Cuts at: 0 1697 4059 Size: 1697 2362 BtgI C'CryG_G Cuts at: 0 735 751 1865 2280 4059 Size: 735 16 1114 415 1779 Fragments arranged by size: 1779 1114 735 415 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 369 4049 4059 Size: 369 3680 10 Fragments arranged by size: 3680 369 10 BtsI GCAGTG_nn' Cuts at: 0 1360 4059 Size: 1360 2699 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1253 1288 4059 Size: 1253 35 2771 Fragments arranged by size: 2771 1253 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2775 4059 Size: 2775 1284 EaeI y'GGCC_r Cuts at: 0 125 258 713 1052 1764 2118 3686 4059 Size: 125 133 455 339 712 354 1568 373 Fragments arranged by size: 1568 712 455 373 354 339 133 125 EagI C'GGCC_G Cuts at: 0 125 258 4059 Size: 125 133 3801 Fragments arranged by size: 3801 133 125 EarI CTCTTCn'nnn_ Cuts at: 0 302 343 559 643 737 1603 2013 2573 Size: 302 41 216 84 94 866 410 560 Cuts at: 2573 3251 4059 Size: 678 808 Fragments arranged by size: 866 808 678 560 410 302 216 94 84 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 597 3700 4059 Size: 597 3103 359 Fragments arranged by size: 3103 597 359 EcoICRI GAG'CTC Cuts at: 0 704 1978 4059 Size: 704 1274 2081 Fragments arranged by size: 2081 1274 704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 252 583 1048 1084 2438 2662 2807 3205 Size: 252 331 465 36 1354 224 145 398 Cuts at: 3205 3272 3307 4059 Size: 67 35 752 Fragments arranged by size: 1354 752 465 398 331 252 224 145 67 36 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 685 4059 Size: 685 3374 EcoO109I rG'GnC_Cy Cuts at: 0 747 3237 3912 4059 Size: 747 2490 675 147 Fragments arranged by size: 2490 747 675 147 FseI GG_CCGG'CC Cuts at: 0 264 4059 Size: 264 3795 FspI TGC'GCA Cuts at: 0 658 4059 Size: 658 3401 HaeII r_GCGC'y Cuts at: 0 137 158 164 2225 4059 Size: 137 21 6 2061 1834 Fragments arranged by size: 2061 1834 137 21 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 30 1015 1047 2992 3024 3163 3195 4059 Size: 30 985 32 1945 32 139 32 864 Fragments arranged by size: 1945 985 864 139 32 32 32 30 HincII GTy'rAC Cuts at: 0 1041 2413 2544 3099 3818 4059 Size: 1041 1372 131 555 719 241 Fragments arranged by size: 1372 1041 719 555 241 131 HindIII A'AGCT_T Cuts at: 0 2632 4059 Size: 2632 1427 HpaI GTT'AAC Cuts at: 0 3099 4059 Size: 3099 960 KasI G'GCGC_C Cuts at: 0 154 160 2221 4059 Size: 154 6 2061 1838 Fragments arranged by size: 2061 1838 154 6 MfeI C'AATT_G Cuts at: 0 1711 2972 4059 Size: 1711 1261 1087 Fragments arranged by size: 1711 1261 1087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 293 1394 2036 4059 Size: 293 1101 642 2023 Fragments arranged by size: 2023 1101 642 293 MscI TGG'CCA Cuts at: 0 715 1054 2120 4059 Size: 715 339 1066 1939 Fragments arranged by size: 1939 1066 715 339 MslI CAynn'nnrTG Cuts at: 0 418 1138 2279 3144 3463 3799 4021 4059 Size: 418 720 1141 865 319 336 222 38 Fragments arranged by size: 1141 865 720 418 336 319 222 38 MspA1I CmG'CkG Cuts at: 0 200 212 360 456 753 888 1369 1941 Size: 200 12 148 96 297 135 481 572 Cuts at: 1941 2856 4059 Size: 915 1203 Fragments arranged by size: 1203 915 572 481 297 200 148 135 96 12 NaeI GCC'GGC Cuts at: 0 129 159 262 430 4059 Size: 129 30 103 168 3629 Fragments arranged by size: 3629 168 129 103 30 NarI GG'CG_CC Cuts at: 0 155 161 2222 4059 Size: 155 6 2061 1837 Fragments arranged by size: 2061 1837 155 6 NcoI C'CATG_G Cuts at: 0 2280 4059 Size: 2280 1779 NgoMIV G'CCGG_C Cuts at: 0 127 157 260 428 4059 Size: 127 30 103 168 3631 Fragments arranged by size: 3631 168 127 103 30 NspI r_CATG'y Cuts at: 0 1014 1071 2713 2764 4059 Size: 1014 57 1642 51 1295 Fragments arranged by size: 1642 1295 1014 57 51 PasI CC'CwG_GG Cuts at: 0 1335 4059 Size: 1335 2724 PciI A'CATG_T Cuts at: 0 1010 2709 4059 Size: 1010 1699 1350 Fragments arranged by size: 1699 1350 1010 PflMI CCAn_nnn'nTGG Cuts at: 0 510 4059 Size: 510 3549 PfoI T'CCnGG_A Cuts at: 0 706 3914 4059 Size: 706 3208 145 Fragments arranged by size: 3208 706 145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 851 883 1150 1182 4059 Size: 851 32 267 32 2877 Fragments arranged by size: 2877 851 267 32 32 PpuMI rG'GwC_Cy Cuts at: 0 747 3237 3912 4059 Size: 747 2490 675 147 Fragments arranged by size: 2490 747 675 147 PshAI GACnn'nnGTC Cuts at: 0 1900 3706 4059 Size: 1900 1806 353 Fragments arranged by size: 1900 1806 353 PstI C_TGCA'G Cuts at: 0 326 359 461 4013 4059 Size: 326 33 102 3552 46 Fragments arranged by size: 3552 326 102 46 33 PvuII CAG'CTG Cuts at: 0 360 456 888 1369 1941 2856 4059 Size: 360 96 432 481 572 915 1203 Fragments arranged by size: 1203 915 572 481 432 360 96 RsrII CG'GwC_CG Cuts at: 0 3822 4059 Size: 3822 237 SacI G_AGCT'C Cuts at: 0 706 1980 4059 Size: 706 1274 2079 Fragments arranged by size: 2079 1274 706 SacII CC_GC'GG Cuts at: 0 754 4059 Size: 754 3305 SalI G'TCGA_C Cuts at: 0 2411 4059 Size: 2411 1648 SapI GCTCTTCn'nnn_ Cuts at: 0 559 4059 Size: 559 3500 SexAI A'CCwGG_T Cuts at: 0 3811 4059 Size: 3811 248 SfcI C'TryA_G Cuts at: 0 322 355 457 1291 2250 3888 4009 4059 Size: 322 33 102 834 959 1638 121 50 Fragments arranged by size: 1638 959 834 322 121 102 50 33 SfoI GGC'GCC Cuts at: 0 156 162 2223 4059 Size: 156 6 2061 1836 Fragments arranged by size: 2061 1836 156 6 SgrAI Cr'CCGG_yG Cuts at: 0 157 4059 Size: 157 3902 SmaI CCC'GGG Cuts at: 0 622 3675 4059 Size: 622 3053 384 Fragments arranged by size: 3053 622 384 SpeI A'CTAG_T Cuts at: 0 2661 4059 Size: 2661 1398 SphI G_CATG'C Cuts at: 0 1071 2764 4059 Size: 1071 1693 1295 Fragments arranged by size: 1693 1295 1071 StuI AGG'CCT Cuts at: 0 1226 2292 4059 Size: 1226 1066 1767 Fragments arranged by size: 1767 1226 1066 StyI C'CwwG_G Cuts at: 0 1221 1919 2280 4059 Size: 1221 698 361 1779 Fragments arranged by size: 1779 1221 698 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 166 1348 4059 Size: 166 1182 2711 Fragments arranged by size: 2711 1182 166 TatI w'GTAC_w Cuts at: 0 1013 1652 2130 3755 4059 Size: 1013 639 478 1625 304 Fragments arranged by size: 1625 1013 639 478 304 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 158 190 1111 1143 1787 1819 4059 Size: 158 32 921 32 644 32 2240 Fragments arranged by size: 2240 921 644 158 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 282 838 1137 4059 Size: 282 556 299 2922 Fragments arranged by size: 2922 556 299 282 XmaI C'CCGG_G Cuts at: 0 620 3673 4059 Size: 620 3053 386 Fragments arranged by size: 3053 620 386 XmnI GAAnn'nnTTC Cuts at: 0 1384 2675 4059 Size: 1384 1291 1384 Fragments arranged by size: 1384 1384 1291 ZraI GAC'GTC Cuts at: 0 940 4059 Size: 940 3119 Enzymes that do cut: AarI AatII AccI AclI AcuI AflIII AgeI AhdI AlfI AloI AlwNI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PstI PvuII RsrII SacI SacII SalI SapI SexAI SfcI SfoI SgrAI SmaI SpeI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AfeI AflII AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BmtI BplI BpuEI BsaI BsaBI BsiWI BssSI BstAPI BstEII BstZ17I ClaI DraI DraIII EcoRI EcoRV FalI FspAI KpnI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI SanDI SbfI ScaI SfiI SgfI SmlI SnaBI SrfI SspI SwaI Tth111I XbaI XhoI