Restriction map for
pF1KSDA1125
(Linear) (Six-base) MAPSORT of: KIAA1125.seq Check: 4521 from: 1 to: 3558
KIAA1125.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AatII G_ACGT'C
Cuts at: 0 3328 3558
Size: 3328 230
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1359 2089 3275 3558
Size: 874 485 730 1186 283
Fragments arranged by size:
1186 874 730 485 283
AflIII A'CryG_T
Cuts at: 0 3481 3558
Size: 3481 77
AgeI A'CCGG_T
Cuts at: 0 2280 3558
Size: 2280 1278
AleI CACnn'nnGTG
Cuts at: 0 1376 2251 2656 2713 3558
Size: 1376 875 405 57 845
Fragments arranged by size:
1376 875 845 405 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 926 1258 3558
Size: 926 332 2300
Fragments arranged by size:
2300 926 332
AlwNI CAG_nnn'CTG
Cuts at: 0 229 941 1978 2916 2985 3134 3199 3558
Size: 229 712 1037 938 69 149 65 359
Fragments arranged by size:
1037 938 712 359 229 149 69 65
ApaLI G'TGCA_C
Cuts at: 0 1318 3558
Size: 1318 2240
ApoI r'AATT_y
Cuts at: 0 980 3558
Size: 980 2578
AvaI C'yCGr_G
Cuts at: 0 320 2694 3270 3558
Size: 320 2374 576 288
Fragments arranged by size:
2374 576 320 288
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3486 3519 3558
Size: 3486 33 39
Fragments arranged by size:
3486 39 33
BanI G'GyrC_C
Cuts at: 0 2320 3558
Size: 2320 1238
BanII G_rGCy'C
Cuts at: 0 314 1330 2635 2933 3268 3558
Size: 314 1016 1305 298 335 290
Fragments arranged by size:
1305 1016 335 314 298 290
BbeI G_GCGC'C
Cuts at: 0 2324 3558
Size: 2324 1234
BbsI GAAGACnn'nnnn_
Cuts at: 0 1850 2162 2555 3558
Size: 1850 312 393 1003
Fragments arranged by size:
1850 1003 393 312
BbvCI CC'TCA_GC
Cuts at: 0 2754 2887 3206 3224 3558
Size: 2754 133 319 18 334
Fragments arranged by size:
2754 334 319 133 18
BfrBI ATG'CAT
Cuts at: 0 413 514 3558
Size: 413 101 3044
Fragments arranged by size:
3044 413 101
BglI GCCn_nnn'nGGC
Cuts at: 0 640 3558
Size: 640 2918
BlpI GC'TnA_GC
Cuts at: 0 1594 3558
Size: 1594 1964
Bme1580I G_kGCm'C
Cuts at: 0 1258 1322 3558
Size: 1258 64 2236
Fragments arranged by size:
2236 1258 64
BmgBI CAC'GTC
Cuts at: 0 1335 1364 1952 2342 2642 3558
Size: 1335 29 588 390 300 916
Fragments arranged by size:
1335 916 588 390 300 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1915 2386 3183 3558
Size: 1915 471 797 375
Fragments arranged by size:
1915 797 471 375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3369 3405 3558
Size: 3369 36 153
Fragments arranged by size:
3369 153 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2000 2893 3007 3052 3360 3558
Size: 2000 893 114 45 308 198
Fragments arranged by size:
2000 893 308 198 114 45
Bpu10I CC'TnA_GC
Cuts at: 0 2006 2754 2887 3167 3206 3224 3558
Size: 2006 748 133 280 39 18 334
Fragments arranged by size:
2006 748 334 280 133 39 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 3558
Size: 457 3101
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 3055 3558
Size: 412 2643 503
Fragments arranged by size:
2643 503 412
BsaAI yAC'GTr
Cuts at: 0 1047 3558
Size: 1047 2511
BsaHI Gr'CG_yC
Cuts at: 0 2321 3325 3385 3558
Size: 2321 1004 60 173
Fragments arranged by size:
2321 1004 173 60
BsaWI w'CCGG_w
Cuts at: 0 2280 3558
Size: 2280 1278
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1312 1342 1510 1540 3444 3474 3558
Size: 1312 30 168 30 1904 30 84
Fragments arranged by size:
1904 1312 168 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1402 1475 1951 3194 3258 3381 3511 3558
Size: 1402 73 476 1243 64 123 130 47
Fragments arranged by size:
1402 1243 476 130 123 73 64 47
BseYI C'CCAG_C
Cuts at: 0 67 272 1150 3558
Size: 67 205 878 2408
Fragments arranged by size:
2408 878 205 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 2461 2902 3168 3558
Size: 609 1852 441 266 390
Fragments arranged by size:
1852 609 441 390 266
BsiEI CG_ry'CG
Cuts at: 0 1748 2438 3558
Size: 1748 690 1120
Fragments arranged by size:
1748 1120 690
BsiHKAI G_wGCw'C
Cuts at: 0 314 1306 1322 1417 1757 2288 2710 2933
Size: 314 992 16 95 340 531 422 223
Cuts at: 2933 3173 3268 3277 3558
Size: 240 95 9 281
Fragments arranged by size:
992 531 422 340 314 281 240 223
95 95 16 9
BsmI GAATG_Cn'
Cuts at: 0 415 514 606 3558
Size: 415 99 92 2952
Fragments arranged by size:
2952 415 99 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2077 3316 3376 3558
Size: 2077 1239 60 182
Fragments arranged by size:
2077 1239 182 60
Bsp1286I G_dGCh'C
Cuts at: 0 314 1258 1306 1322 1330 1417 1757 2288
Size: 314 944 48 16 8 87 340 531
Cuts at: 2288 2635 2710 2933 3173 3268 3277 3558
Size: 347 75 223 240 95 9 281
Fragments arranged by size:
944 531 347 340 314 281 240 223
95 87 75 48 16 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 894 1652 2744 3558
Size: 476 418 758 1092 814
Fragments arranged by size:
1092 814 758 476 418
BsrBI CCG'CTC
Cuts at: 0 2294 2997 3558
Size: 2294 703 561
Fragments arranged by size:
2294 703 561
BsrFI r'CCGG_y
Cuts at: 0 1156 1534 2280 3002 3558
Size: 1156 378 746 722 556
Fragments arranged by size:
1156 746 722 556 378
BsrGI T'GTAC_A
Cuts at: 0 586 3558
Size: 586 2972
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2508 3558
Size: 2508 1050
BstEII G'GTnAC_C
Cuts at: 0 2352 2658 2685 3558
Size: 2352 306 27 873
Fragments arranged by size:
2352 873 306 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2253 2655 3558
Size: 2253 402 903
Fragments arranged by size:
2253 903 402
BstYI r'GATC_y
Cuts at: 0 24 1223 3558
Size: 24 1199 2335
Fragments arranged by size:
2335 1199 24
BtgI C'CryG_G
Cuts at: 0 1394 2312 2348 2963 3558
Size: 1394 918 36 615 595
Fragments arranged by size:
1394 918 615 595 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2660 2727 2994 3558
Size: 2660 67 267 564
Fragments arranged by size:
2660 564 267 67
BtsI GCAGTG_nn'
Cuts at: 0 1160 2747 3082 3465 3558
Size: 1160 1587 335 383 93
Fragments arranged by size:
1587 1160 383 335 93
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 818 853 1904 1939 3330 3365 3558
Size: 818 35 1051 35 1391 35 193
Fragments arranged by size:
1391 1051 818 193 35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 904 3558
Size: 904 2654
EaeI y'GGCC_r
Cuts at: 0 90 1745 2435 3299 3558
Size: 90 1655 690 864 259
Fragments arranged by size:
1655 864 690 259 90
EagI C'GGCC_G
Cuts at: 0 1745 2435 3558
Size: 1745 690 1123
Fragments arranged by size:
1745 1123 690
EarI CTCTTCn'nnn_
Cuts at: 0 1317 2301 3463 3508 3558
Size: 1317 984 1162 45 50
Fragments arranged by size:
1317 1162 984 50 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2289 2983 3558
Size: 2289 694 575
Fragments arranged by size:
2289 694 575
EcoICRI GAG'CTC
Cuts at: 0 312 2931 3266 3558
Size: 312 2619 335 292
Fragments arranged by size:
2619 335 312 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1359 2000 2089 2893 3007 3052 3275
Size: 874 485 641 89 804 114 45 223
Cuts at: 3275 3360 3558
Size: 85 198
Fragments arranged by size:
874 804 641 485 223 198 114 89
85 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2989 3558
Size: 2989 569
EcoO109I rG'GnC_Cy
Cuts at: 0 938 3558
Size: 938 2620
EcoRV GAT'ATC
Cuts at: 0 6 2581 3558
Size: 6 2575 977
Fragments arranged by size:
2575 977 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1029 1215 3558
Size: 1029 186 2343
Fragments arranged by size:
2343 1029 186
HaeII r_GCGC'y
Cuts at: 0 1157 1650 2324 3308 3558
Size: 1157 493 674 984 250
Fragments arranged by size:
1157 984 674 493 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1510 1542 1810 1842 2499 2531
Size: 16 32 1462 32 268 32 657 32
Cuts at: 2531 2565 2597 3558
Size: 34 32 961
Fragments arranged by size:
1462 961 657 268 34 32 32 32
32 32 16
HincII GTy'rAC
Cuts at: 0 2727 3558
Size: 2727 831
KasI G'GCGC_C
Cuts at: 0 2320 3558
Size: 2320 1238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 1355 3017 3100 3430 3434 3558
Size: 1259 96 1662 83 330 4 124
Fragments arranged by size:
1662 1259 330 124 96 83 4
MscI TGG'CCA
Cuts at: 0 92 3558
Size: 92 3466
MslI CAynn'nnrTG
Cuts at: 0 439 1376 2251 2656 2713 3558
Size: 439 937 875 405 57 845
Fragments arranged by size:
937 875 845 439 405 57
MspA1I CmG'CkG
Cuts at: 0 1671 2314 2445 2758 2770 3030 3331 3558
Size: 1671 643 131 313 12 260 301 227
Fragments arranged by size:
1671 643 313 301 260 227 131 12
NaeI GCC'GGC
Cuts at: 0 1158 3558
Size: 1158 2400
NarI GG'CG_CC
Cuts at: 0 2321 3558
Size: 2321 1237
NcoI C'CATG_G
Cuts at: 0 1394 2963 3558
Size: 1394 1569 595
Fragments arranged by size:
1569 1394 595
NgoMIV G'CCGG_C
Cuts at: 0 1156 3558
Size: 1156 2402
NsiI A_TGCA'T
Cuts at: 0 415 516 3558
Size: 415 101 3042
Fragments arranged by size:
3042 415 101
NspI r_CATG'y
Cuts at: 0 2502 3558
Size: 2502 1056
PasI CC'CwG_GG
Cuts at: 0 2568 3258 3558
Size: 2568 690 300
Fragments arranged by size:
2568 690 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 3558
Size: 576 2982
PfoI T'CCnGG_A
Cuts at: 0 1403 3430 3558
Size: 1403 2027 128
Fragments arranged by size:
2027 1403 128
PsiI TTA'TAA
Cuts at: 0 680 3558
Size: 680 2878
PspXI vC'TCGA_Gb
Cuts at: 0 3270 3558
Size: 3270 288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3115 3147 3558
Size: 3115 32 411
Fragments arranged by size:
3115 411 32
PstI C_TGCA'G
Cuts at: 0 39 3558
Size: 39 3519
PvuII CAG'CTG
Cuts at: 0 1671 2758 3030 3331 3558
Size: 1671 1087 272 301 227
Fragments arranged by size:
1671 1087 301 272 227
SacI G_AGCT'C
Cuts at: 0 314 2933 3268 3558
Size: 314 2619 335 290
Fragments arranged by size:
2619 335 314 290
SacII CC_GC'GG
Cuts at: 0 2315 3558
Size: 2315 1243
SapI GCTCTTCn'nnn_
Cuts at: 0 2301 3508 3558
Size: 2301 1207 50
Fragments arranged by size:
2301 1207 50
SfcI C'TryA_G
Cuts at: 0 35 3558
Size: 35 3523
SfoI GGC'GCC
Cuts at: 0 2322 3558
Size: 2322 1236
SgrAI Cr'CCGG_yG
Cuts at: 0 2280 3558
Size: 2280 1278
SmaI CCC'GGG
Cuts at: 0 322 3558
Size: 322 3236
SmlI C'TyrA_G
Cuts at: 0 472 3270 3558
Size: 472 2798 288
Fragments arranged by size:
2798 472 288
StyI C'CwwG_G
Cuts at: 0 1394 2963 3414 3558
Size: 1394 1569 451 144
Fragments arranged by size:
1569 1394 451 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1125 1593 3202 3558
Size: 1125 468 1609 356
Fragments arranged by size:
1609 1125 468 356
TatI w'GTAC_w
Cuts at: 0 586 2783 3558
Size: 586 2197 775
Fragments arranged by size:
2197 775 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1312 1344 1611 1643 2378 2410 3163 3195
Size: 1312 32 267 32 735 32 753 32
Cuts at: 3195 3444 3476 3558
Size: 249 32 82
Fragments arranged by size:
1312 753 735 267 249 82 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 188 3558
Size: 188 3370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 368 1032 1401 2652 3558
Size: 368 664 369 1251 906
Fragments arranged by size:
1251 906 664 369 368
XhoI C'TCGA_G
Cuts at: 0 3270 3558
Size: 3270 288
XmaI C'CCGG_G
Cuts at: 0 320 3558
Size: 320 3238
XmnI GAAnn'nnTTC
Cuts at: 0 262 3558
Size: 262 3296
ZraI GAC'GTC
Cuts at: 0 3326 3558
Size: 3326 232
Enzymes that do cut:
AatII AcuI AflIII AgeI AleI AlfI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PsiI
PspXI PsrI PstI PvuII SacI SacII SapI SfcI
SfoI SgrAI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AloI
ApaI AscI AseI AsiSI AvrII BamHI BcgI BciVI
BclI BglII BmtI BsaBI BsiWI BspEI BspHI BsrDI
BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI DraIII
EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI