Restriction map for
pF1KA1128
(Linear) (Six-base) MAPSORT of: KIAA1128.seq Check: 6382 from: 1 to: 3141
KIAA1128.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1035 3141
Size: 1035 2106
AccI GT'mk_AC
Cuts at: 0 2690 3141
Size: 2690 451
Acc65I G'GTAC_C
Cuts at: 0 2793 3141
Size: 2793 348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1362 2212 2591 2647 2866 3141
Size: 1362 850 379 56 219 275
Fragments arranged by size:
1362 850 379 275 219 56
AflIII A'CryG_T
Cuts at: 0 291 1036 1532 2475 2863 3141
Size: 291 745 496 943 388 278
Fragments arranged by size:
943 745 496 388 291 278
AlwNI CAG_nnn'CTG
Cuts at: 0 2809 2925 3141
Size: 2809 116 216
Fragments arranged by size:
2809 216 116
ApaLI G'TGCA_C
Cuts at: 0 224 2450 3141
Size: 224 2226 691
Fragments arranged by size:
2226 691 224
ApoI r'AATT_y
Cuts at: 0 258 451 687 816 1020 2318 3141
Size: 258 193 236 129 204 1298 823
Fragments arranged by size:
1298 823 258 236 204 193 129
AseI AT'TA_AT
Cuts at: 0 638 3141
Size: 638 2503
AvaI C'yCGr_G
Cuts at: 0 2674 3141
Size: 2674 467
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1155 1188 3141
Size: 1155 33 1953
Fragments arranged by size:
1953 1155 33
BanI G'GyrC_C
Cuts at: 0 2793 3141
Size: 2793 348
BbsI GAAGACnn'nnnn_
Cuts at: 0 1241 1271 3112 3141
Size: 1241 30 1841 29
Fragments arranged by size:
1841 1241 30 29
BbvCI CC'TCA_GC
Cuts at: 0 1819 3141
Size: 1819 1322
BfrBI ATG'CAT
Cuts at: 0 2439 3141
Size: 2439 702
BlpI GC'TnA_GC
Cuts at: 0 606 3141
Size: 606 2535
Bme1580I G_kGCm'C
Cuts at: 0 228 2454 3141
Size: 228 2226 687
Fragments arranged by size:
2226 687 228
BmgBI CAC'GTC
Cuts at: 0 2478 3141
Size: 2478 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1274 2331 2786 3141
Size: 617 657 1057 455 355
Fragments arranged by size:
1057 657 617 455 355
Bpu10I CC'TnA_GC
Cuts at: 0 1067 1819 3141
Size: 1067 752 1322
Fragments arranged by size:
1322 1067 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1666 1881 2312 2585 3141
Size: 287 1379 215 431 273 556
Fragments arranged by size:
1379 556 431 287 273 215
BsaBI GATnn'nnATC
Cuts at: 0 1655 1673 3141
Size: 1655 18 1468
Fragments arranged by size:
1655 1468 18
BsaWI w'CCGG_w
Cuts at: 0 2457 3141
Size: 2457 684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1413 1443 3141
Size: 1413 30 1698
Fragments arranged by size:
1698 1413 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 2315 3141
Size: 421 1894 826
Fragments arranged by size:
1894 826 421
BsiHKAI G_wGCw'C
Cuts at: 0 228 2454 3141
Size: 228 2226 687
Fragments arranged by size:
2226 687 228
BsmI GAATG_Cn'
Cuts at: 0 2425 3081 3141
Size: 2425 656 60
Fragments arranged by size:
2425 656 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2056 2307 3141
Size: 2056 251 834
Fragments arranged by size:
2056 834 251
Bsp1286I G_dGCh'C
Cuts at: 0 228 2454 3141
Size: 228 2226 687
Fragments arranged by size:
2226 687 228
BspEI T'CCGG_A
Cuts at: 0 2457 3141
Size: 2457 684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1035 3141
Size: 1035 2106
BsrDI GCAATG_nn'
Cuts at: 0 76 2080 2101 3141
Size: 76 2004 21 1040
Fragments arranged by size:
2004 1040 76 21
BsrGI T'GTAC_A
Cuts at: 0 1429 3141
Size: 1429 1712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 2420 3141
Size: 87 2333 721
Fragments arranged by size:
2333 721 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1579 3141
Size: 1579 1562
BstYI r'GATC_y
Cuts at: 0 744 1674 2210 3141
Size: 744 930 536 931
Fragments arranged by size:
931 930 744 536
Bsu36I CC'TnA_GG
Cuts at: 0 1682 1760 2952 3141
Size: 1682 78 1192 189
Fragments arranged by size:
1682 1192 189 78
BtgI C'CryG_G
Cuts at: 0 1845 3141
Size: 1845 1296
BtsI GCAGTG_nn'
Cuts at: 0 2015 2421 3141
Size: 2015 406 720
Fragments arranged by size:
2015 720 406
ClaI AT'CG_AT
Cuts at: 0 3108 3141
Size: 3108 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 372 407 2531 2566 3141
Size: 372 35 2124 35 575
Fragments arranged by size:
2124 575 372 35 35
DraI TTT'AAA
Cuts at: 0 2768 3141
Size: 2768 373
DraIII CAC_nnn'GTG
Cuts at: 0 232 3141
Size: 232 2909
EarI CTCTTCn'nnn_
Cuts at: 0 233 1307 2504 2666 3141
Size: 233 1074 1197 162 475
Fragments arranged by size:
1197 1074 475 233 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2714 2932 3141
Size: 2714 218 209
Fragments arranged by size:
2714 218 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1274 1362 2212 2331 2591 2647 2786
Size: 617 657 88 850 119 260 56 139
Cuts at: 2786 2866 3141
Size: 80 275
Fragments arranged by size:
850 657 617 275 260 139 119 88
80 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 302 1763 3141
Size: 302 1461 1378
Fragments arranged by size:
1461 1378 302
EcoRI G'AATT_C
Cuts at: 0 687 2318 3141
Size: 687 1631 823
Fragments arranged by size:
1631 823 687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 368 3141
Size: 368 2773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 486 518 1285 1317 3141
Size: 160 32 294 32 767 32 1824
Fragments arranged by size:
1824 767 294 160 32 32 32
HindIII A'AGCT_T
Cuts at: 0 603 2466 3003 3141
Size: 603 1863 537 138
Fragments arranged by size:
1863 603 537 138
KpnI G_GTAC'C
Cuts at: 0 2797 3141
Size: 2797 344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 2238 2688 3141
Size: 1295 943 450 453
Fragments arranged by size:
1295 943 453 450
MslI CAynn'nnrTG
Cuts at: 0 784 1325 1533 1844 2063 3141
Size: 784 541 208 311 219 1078
Fragments arranged by size:
1078 784 541 311 219 208
MspA1I CmG'CkG
Cuts at: 0 544 3141
Size: 544 2597
NcoI C'CATG_G
Cuts at: 0 1845 3141
Size: 1845 1296
NsiI A_TGCA'T
Cuts at: 0 2441 3141
Size: 2441 700
NspI r_CATG'y
Cuts at: 0 555 1040 1536 2439 2643 2867 3141
Size: 555 485 496 903 204 224 274
Fragments arranged by size:
903 555 496 485 274 224 204
PasI CC'CwG_GG
Cuts at: 0 2376 3141
Size: 2376 765
PciI A'CATG_T
Cuts at: 0 1036 1532 2863 3141
Size: 1036 496 1331 278
Fragments arranged by size:
1331 1036 496 278
PflMI CCAn_nnn'nTGG
Cuts at: 0 52 991 2128 3141
Size: 52 939 1137 1013
Fragments arranged by size:
1137 1013 939 52
PvuII CAG'CTG
Cuts at: 0 544 3141
Size: 544 2597
SapI GCTCTTCn'nnn_
Cuts at: 0 233 2504 3141
Size: 233 2271 637
Fragments arranged by size:
2271 637 233
ScaI AGT'ACT
Cuts at: 0 836 2333 3141
Size: 836 1497 808
Fragments arranged by size:
1497 836 808
SfcI C'TryA_G
Cuts at: 0 401 1055 1476 1878 2335 3141
Size: 401 654 421 402 457 806
Fragments arranged by size:
806 654 457 421 402 401
SmlI C'TyrA_G
Cuts at: 0 302 1645 1860 2327 2600 3141
Size: 302 1343 215 467 273 541
Fragments arranged by size:
1343 541 467 302 273 215
SphI G_CATG'C
Cuts at: 0 555 3141
Size: 555 2586
StuI AGG'CCT
Cuts at: 0 561 876 3141
Size: 561 315 2265
Fragments arranged by size:
2265 561 315
StyI C'CwwG_G
Cuts at: 0 1358 1845 3028 3141
Size: 1358 487 1183 113
Fragments arranged by size:
1358 1183 487 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1748 2389 3141
Size: 1748 641 752
Fragments arranged by size:
1748 752 641
TatI w'GTAC_w
Cuts at: 0 73 834 1429 2331 2560 2683 3141
Size: 73 761 595 902 229 123 458
Fragments arranged by size:
902 761 595 458 229 123 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1790 1795 3141
Size: 1790 5 1346
Fragments arranged by size:
1790 1346 5
XmnI GAAnn'nnTTC
Cuts at: 0 480 591 691 2076 3141
Size: 480 111 100 1385 1065
Fragments arranged by size:
1385 1065 480 111 100
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApaLI ApoI
AseI AvaI BaeI BanI BbsI BbvCI BfrBI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraI DraIII EarI EciI Eco57MI EcoNI EcoRI
FalI Hin4I HindIII KpnI MmeI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PvuII SapI ScaI
SfcI SmlI SphI StuI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvrII BamHI BanII BbeI
BcgI BciVI BclI BglI BglII BmrI BmtI BplI
BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiWI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
DrdI EaeI EagI EcoICRI EcoO109I EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI