Restriction map for
pF1KSDA1129
(Linear) (Six-base) MAPSORT of: KIAA1129.seq Check: 1372 from: 1 to: 1761
KIAA1129.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 496 1009 1723 1761
Size: 496 513 714 38
Fragments arranged by size:
714 513 496 38
AatII G_ACGT'C
Cuts at: 0 1126 1761
Size: 1126 635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 608 1761
Size: 368 240 1153
Fragments arranged by size:
1153 368 240
AflIII A'CryG_T
Cuts at: 0 245 711 1043 1571 1761
Size: 245 466 332 528 190
Fragments arranged by size:
528 466 332 245 190
AgeI A'CCGG_T
Cuts at: 0 974 1761
Size: 974 787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 1761
Size: 530 1231
AlwNI CAG_nnn'CTG
Cuts at: 0 36 300 366 1368 1761
Size: 36 264 66 1002 393
Fragments arranged by size:
1002 393 264 66 36
ApoI r'AATT_y
Cuts at: 0 559 1761
Size: 559 1202
AvrII C'CTAG_G
Cuts at: 0 733 1761
Size: 733 1028
BamHI G'GATC_C
Cuts at: 0 1639 1761
Size: 1639 122
BanI G'GyrC_C
Cuts at: 0 927 1761
Size: 927 834
BanII G_rGCy'C
Cuts at: 0 28 1613 1761
Size: 28 1585 148
Fragments arranged by size:
1585 148 28
BglI GCCn_nnn'nGGC
Cuts at: 0 1442 1761
Size: 1442 319
BlpI GC'TnA_GC
Cuts at: 0 22 992 1462 1761
Size: 22 970 470 299
Fragments arranged by size:
970 470 299 22
BmgBI CAC'GTC
Cuts at: 0 153 285 1761
Size: 153 132 1476
Fragments arranged by size:
1476 153 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 1168 1761
Size: 1168 593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1761
Size: 869 892
BsaAI yAC'GTr
Cuts at: 0 1044 1416 1761
Size: 1044 372 345
Fragments arranged by size:
1044 372 345
BsaBI GATnn'nnATC
Cuts at: 0 274 1761
Size: 274 1487
BsaHI Gr'CG_yC
Cuts at: 0 1123 1761
Size: 1123 638
BsaWI w'CCGG_w
Cuts at: 0 974 1761
Size: 974 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 961 991 1761
Size: 961 30 770
Fragments arranged by size:
961 770 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 820 982 1761
Size: 820 162 779
Fragments arranged by size:
820 779 162
BseYI C'CCAG_C
Cuts at: 0 46 1392 1761
Size: 46 1346 369
Fragments arranged by size:
1346 369 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 609 1619 1761
Size: 474 135 1010 142
Fragments arranged by size:
1010 474 142 135
BsiHKAI G_wGCw'C
Cuts at: 0 28 692 1156 1175 1375 1432 1717 1761
Size: 28 664 464 19 200 57 285 44
Fragments arranged by size:
664 464 285 200 57 44 28 19
BsmI GAATG_Cn'
Cuts at: 0 106 1761
Size: 106 1655
Bsp1286I G_dGCh'C
Cuts at: 0 28 692 1156 1175 1375 1432 1613 1717
Size: 28 664 464 19 200 57 181 104
Cuts at: 1717 1761
Size: 44
Fragments arranged by size:
664 464 200 181 104 57 44 28
19
BspHI T'CATG_A
Cuts at: 0 905 1761
Size: 905 856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 496 1009 1723 1761
Size: 496 513 714 38
Fragments arranged by size:
714 513 496 38
BsrDI GCAATG_nn'
Cuts at: 0 1127 1761
Size: 1127 634
BsrFI r'CCGG_y
Cuts at: 0 974 1577 1761
Size: 974 603 184
Fragments arranged by size:
974 603 184
BsrGI T'GTAC_A
Cuts at: 0 480 1761
Size: 480 1281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1738 1761
Size: 1738 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 1761
Size: 456 1305
BstYI r'GATC_y
Cuts at: 0 1639 1761
Size: 1639 122
BtgI C'CryG_G
Cuts at: 0 1052 1761
Size: 1052 709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 244 1761
Size: 244 1517
BtsI GCAGTG_nn'
Cuts at: 0 951 1072 1199 1302 1446 1761
Size: 951 121 127 103 144 315
Fragments arranged by size:
951 315 144 127 121 103
DraIII CAC_nnn'GTG
Cuts at: 0 195 1761
Size: 195 1566
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 1761
Size: 745 1016
EaeI y'GGCC_r
Cuts at: 0 921 1038 1761
Size: 921 117 723
Fragments arranged by size:
921 723 117
EarI CTCTTCn'nnn_
Cuts at: 0 344 623 1507 1761
Size: 344 279 884 254
Fragments arranged by size:
884 344 279 254
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1305 1435 1737 1761
Size: 1305 130 302 24
Fragments arranged by size:
1305 302 130 24
EcoICRI GAG'CTC
Cuts at: 0 26 1761
Size: 26 1735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 608 869 1761
Size: 368 240 261 892
Fragments arranged by size:
892 368 261 240
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 898 1761
Size: 808 90 863
Fragments arranged by size:
863 808 90
EcoRV GAT'ATC
Cuts at: 0 599 1761
Size: 599 1162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 580 1761
Size: 580 1181
HaeII r_GCGC'y
Cuts at: 0 1149 1761
Size: 1149 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 710 742 1624 1656 1761
Size: 710 32 882 32 105
Fragments arranged by size:
882 710 105 32 32
HincII GTy'rAC
Cuts at: 0 84 1761
Size: 84 1677
HindIII A'AGCT_T
Cuts at: 0 767 1761
Size: 767 994
MfeI C'AATT_G
Cuts at: 0 450 1761
Size: 450 1311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 1434 1605 1726 1761
Size: 741 693 171 121 35
Fragments arranged by size:
741 693 171 121 35
MscI TGG'CCA
Cuts at: 0 1040 1761
Size: 1040 721
MslI CAynn'nnrTG
Cuts at: 0 910 1046 1570 1761
Size: 910 136 524 191
Fragments arranged by size:
910 524 191 136
MspA1I CmG'CkG
Cuts at: 0 141 1168 1473 1761
Size: 141 1027 305 288
Fragments arranged by size:
1027 305 288 141
NaeI GCC'GGC
Cuts at: 0 1579 1761
Size: 1579 182
NgoMIV G'CCGG_C
Cuts at: 0 1577 1761
Size: 1577 184
NspI r_CATG'y
Cuts at: 0 249 715 1140 1575 1761
Size: 249 466 425 435 186
Fragments arranged by size:
466 435 425 249 186
PciI A'CATG_T
Cuts at: 0 245 711 1571 1761
Size: 245 466 860 190
Fragments arranged by size:
860 466 245 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 1259 1406 1761
Size: 195 1064 147 355
Fragments arranged by size:
1064 355 195 147
PfoI T'CCnGG_A
Cuts at: 0 1533 1761
Size: 1533 228
PmlI CAC'GTG
Cuts at: 0 1044 1761
Size: 1044 717
PshAI GACnn'nnGTC
Cuts at: 0 886 1761
Size: 886 875
PstI C_TGCA'G
Cuts at: 0 214 392 431 506 1761
Size: 214 178 39 75 1255
Fragments arranged by size:
1255 214 178 75 39
PvuII CAG'CTG
Cuts at: 0 141 1473 1761
Size: 141 1332 288
Fragments arranged by size:
1332 288 141
SacI G_AGCT'C
Cuts at: 0 28 1761
Size: 28 1733
SapI GCTCTTCn'nnn_
Cuts at: 0 1507 1761
Size: 1507 254
ScaI AGT'ACT
Cuts at: 0 1215 1666 1761
Size: 1215 451 95
Fragments arranged by size:
1215 451 95
SexAI A'CCwGG_T
Cuts at: 0 581 1761
Size: 581 1180
SfcI C'TryA_G
Cuts at: 0 210 388 427 502 1239 1761
Size: 210 178 39 75 737 522
Fragments arranged by size:
737 522 210 178 75 39
SphI G_CATG'C
Cuts at: 0 1140 1761
Size: 1140 621
StyI C'CwwG_G
Cuts at: 0 733 865 899 932 1761
Size: 733 132 34 33 829
Fragments arranged by size:
829 733 132 34 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 945 1291 1761
Size: 945 346 470
Fragments arranged by size:
945 470 346
TatI w'GTAC_w
Cuts at: 0 480 1213 1385 1664 1761
Size: 480 733 172 279 97
Fragments arranged by size:
733 480 279 172 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 571 603 961 993 1761
Size: 571 32 358 32 768
Fragments arranged by size:
768 571 358 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 1761
Size: 453 1308
XmnI GAAnn'nnTTC
Cuts at: 0 106 560 1761
Size: 106 454 1201
Fragments arranged by size:
1201 454 106
ZraI GAC'GTC
Cuts at: 0 1124 1761
Size: 1124 637
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AlfI AlwNI ApoI
AvrII BamHI BanI BanII BglI BlpI BmgBI BmrI
BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRV
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI
PmlI PshAI PstI PvuII SacI SapI ScaI SexAI
SfcI SphI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmtI BplI Bpu10I BpuEI BsaI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI EagI EcoO109I EcoRI FseI FspI
FspAI HpaI KasI KpnI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI