Restriction map for
pF1KSDA1135
(Linear) (Six-base) MAPSORT of: KIAA1135.seq Check: 241 from: 1 to: 2268
KIAA1135.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1323 2268
Size: 1323 945
AccI GT'mk_AC
Cuts at: 0 1423 2268
Size: 1423 845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 2228 2268
Size: 1478 750 40
Fragments arranged by size:
1478 750 40
AflII C'TTAA_G
Cuts at: 0 733 856 2268
Size: 733 123 1412
Fragments arranged by size:
1412 733 123
AflIII A'CryG_T
Cuts at: 0 2210 2268
Size: 2210 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1355 1891 2268
Size: 1355 536 377
Fragments arranged by size:
1355 536 377
AlwNI CAG_nnn'CTG
Cuts at: 0 1605 1707 2268
Size: 1605 102 561
Fragments arranged by size:
1605 561 102
ApaI G_GGCC'C
Cuts at: 0 2181 2268
Size: 2181 87
ApaLI G'TGCA_C
Cuts at: 0 665 2268
Size: 665 1603
ApoI r'AATT_y
Cuts at: 0 1504 1642 2268
Size: 1504 138 626
Fragments arranged by size:
1504 626 138
AvaI C'yCGr_G
Cuts at: 0 76 107 2268
Size: 76 31 2161
Fragments arranged by size:
2161 76 31
BanI G'GyrC_C
Cuts at: 0 280 294 1335 2268
Size: 280 14 1041 933
Fragments arranged by size:
1041 933 280 14
BanII G_rGCy'C
Cuts at: 0 77 106 692 1368 2181 2268
Size: 77 29 586 676 813 87
Fragments arranged by size:
813 676 586 87 77 29
BbeI G_GCGC'C
Cuts at: 0 284 298 2268
Size: 284 14 1970
Fragments arranged by size:
1970 284 14
BfrBI ATG'CAT
Cuts at: 0 1692 2268
Size: 1692 576
BglI GCCn_nnn'nGGC
Cuts at: 0 23 1617 2268
Size: 23 1594 651
Fragments arranged by size:
1594 651 23
BlpI GC'TnA_GC
Cuts at: 0 118 2268
Size: 118 2150
Bme1580I G_kGCm'C
Cuts at: 0 669 810 2181 2268
Size: 669 141 1371 87
Fragments arranged by size:
1371 669 141 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 322 813 1648 2268
Size: 322 491 835 620
Fragments arranged by size:
835 620 491 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 291 1492 2041 2106 2268
Size: 145 146 1201 549 65 162
Fragments arranged by size:
1201 549 162 146 145 65
Bpu10I CC'TnA_GC
Cuts at: 0 5 2268
Size: 5 2263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1657 2268
Size: 1657 611
BsaHI Gr'CG_yC
Cuts at: 0 281 295 2268
Size: 281 14 1973
Fragments arranged by size:
1973 281 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1088 1118 1465 1495 2268
Size: 157 30 901 30 347 30 773
Fragments arranged by size:
901 773 347 157 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 178 1180 1348 2007 2268
Size: 142 36 1002 168 659 261
Fragments arranged by size:
1002 659 261 168 142 36
BseYI C'CCAG_C
Cuts at: 0 1529 2268
Size: 1529 739
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 1399 2268
Size: 1325 74 869
Fragments arranged by size:
1325 869 74
BsiEI CG_ry'CG
Cuts at: 0 98 254 305 2268
Size: 98 156 51 1963
Fragments arranged by size:
1963 156 98 51
BsiHKAI G_wGCw'C
Cuts at: 0 669 692 1011 1858 2092 2268
Size: 669 23 319 847 234 176
Fragments arranged by size:
847 669 319 234 176 23
BsmI GAATG_Cn'
Cuts at: 0 1666 2268
Size: 1666 602
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1533 1551 2268
Size: 1533 18 717
Fragments arranged by size:
1533 717 18
Bsp1286I G_dGCh'C
Cuts at: 0 77 106 669 692 810 1011 1368 1858
Size: 77 29 563 23 118 201 357 490
Cuts at: 1858 2092 2181 2268
Size: 234 89 87
Fragments arranged by size:
563 490 357 234 201 118 89 87
77 29 23
BspHI T'CATG_A
Cuts at: 0 596 1011 2268
Size: 596 415 1257
Fragments arranged by size:
1257 596 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1323 2268
Size: 1323 945
BsrBI CCG'CTC
Cuts at: 0 873 1631 2268
Size: 873 758 637
Fragments arranged by size:
873 758 637
BsrDI GCAATG_nn'
Cuts at: 0 513 1196 2003 2268
Size: 513 683 807 265
Fragments arranged by size:
807 683 513 265
BsrFI r'CCGG_y
Cuts at: 0 85 1848 2147 2268
Size: 85 1763 299 121
Fragments arranged by size:
1763 299 121 85
BsrGI T'GTAC_A
Cuts at: 0 143 2268
Size: 143 2125
BssHII G'CGCG_C
Cuts at: 0 207 2268
Size: 207 2061
BstAPI GCAn_nnn'nTGC
Cuts at: 0 673 1605 2268
Size: 673 932 663
Fragments arranged by size:
932 673 663
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 2268
Size: 489 1779
BstYI r'GATC_y
Cuts at: 0 131 260 1036 2268
Size: 131 129 776 1232
Fragments arranged by size:
1232 776 131 129
BtgI C'CryG_G
Cuts at: 0 37 67 299 1090 1096 2093 2268
Size: 37 30 232 791 6 997 175
Fragments arranged by size:
997 791 232 175 37 30 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 776 2268
Size: 776 1492
BtsI GCAGTG_nn'
Cuts at: 0 56 1976 2268
Size: 56 1920 292
Fragments arranged by size:
1920 292 56
ClaI AT'CG_AT
Cuts at: 0 470 2268
Size: 470 1798
DraI TTT'AAA
Cuts at: 0 1052 2268
Size: 1052 1216
DraIII CAC_nnn'GTG
Cuts at: 0 243 2268
Size: 243 2025
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 2268
Size: 283 1985
EaeI y'GGCC_r
Cuts at: 0 95 251 302 479 1093 1195 1376 1990
Size: 95 156 51 177 614 102 181 614
Cuts at: 1990 2165 2268
Size: 175 103
Fragments arranged by size:
614 614 181 177 175 156 103 102
95 51
EagI C'GGCC_G
Cuts at: 0 95 251 302 2268
Size: 95 156 51 1966
Fragments arranged by size:
1966 156 95 51
EarI CTCTTCn'nnn_
Cuts at: 0 779 1454 1910 2268
Size: 779 675 456 358
Fragments arranged by size:
779 675 456 358
EcoICRI GAG'CTC
Cuts at: 0 690 2268
Size: 690 1578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 291 1478 1492 2041 2106 2228 2268
Size: 145 146 1187 14 549 65 122 40
Fragments arranged by size:
1187 549 146 145 122 65 40 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1711 2268
Size: 1711 557
EcoO109I rG'GnC_Cy
Cuts at: 0 1704 1924 2177 2224 2268
Size: 1704 220 253 47 44
Fragments arranged by size:
1704 253 220 47 44
EcoRI G'AATT_C
Cuts at: 0 1642 2268
Size: 1642 626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 748 1797 2268
Size: 748 1049 471
Fragments arranged by size:
1049 748 471
HaeII r_GCGC'y
Cuts at: 0 284 298 2268
Size: 284 14 1970
Fragments arranged by size:
1970 284 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1465 1497 2268
Size: 1465 32 771
Fragments arranged by size:
1465 771 32
HincII GTy'rAC
Cuts at: 0 1424 2268
Size: 1424 844
KasI G'GCGC_C
Cuts at: 0 280 294 2268
Size: 280 14 1974
Fragments arranged by size:
1974 280 14
MscI TGG'CCA
Cuts at: 0 481 2167 2268
Size: 481 1686 101
Fragments arranged by size:
1686 481 101
MslI CAynn'nnrTG
Cuts at: 0 2098 2268
Size: 2098 170
MspA1I CmG'CkG
Cuts at: 0 39 69 301 1347 2268
Size: 39 30 232 1046 921
Fragments arranged by size:
1046 921 232 39 30
NaeI GCC'GGC
Cuts at: 0 87 2149 2268
Size: 87 2062 119
Fragments arranged by size:
2062 119 87
NarI GG'CG_CC
Cuts at: 0 281 295 2268
Size: 281 14 1973
Fragments arranged by size:
1973 281 14
NcoI C'CATG_G
Cuts at: 0 2093 2268
Size: 2093 175
NgoMIV G'CCGG_C
Cuts at: 0 85 2147 2268
Size: 85 2062 121
Fragments arranged by size:
2062 121 85
NsiI A_TGCA'T
Cuts at: 0 1694 2268
Size: 1694 574
NspI r_CATG'y
Cuts at: 0 1209 2214 2268
Size: 1209 1005 54
Fragments arranged by size:
1209 1005 54
PciI A'CATG_T
Cuts at: 0 2210 2268
Size: 2210 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 1707 2268
Size: 1707 561
PfoI T'CCnGG_A
Cuts at: 0 1469 2268
Size: 1469 799
PpuMI rG'GwC_Cy
Cuts at: 0 1704 1924 2268
Size: 1704 220 344
Fragments arranged by size:
1704 344 220
PsiI TTA'TAA
Cuts at: 0 401 2268
Size: 401 1867
PspOMI G'GGCC_C
Cuts at: 0 2177 2268
Size: 2177 91
PstI C_TGCA'G
Cuts at: 0 531 2268
Size: 531 1737
SacI G_AGCT'C
Cuts at: 0 692 2268
Size: 692 1576
SacII CC_GC'GG
Cuts at: 0 40 70 302 2268
Size: 40 30 232 1966
Fragments arranged by size:
1966 232 40 30
SalI G'TCGA_C
Cuts at: 0 1422 2268
Size: 1422 846
SapI GCTCTTCn'nnn_
Cuts at: 0 779 1454 2268
Size: 779 675 814
Fragments arranged by size:
814 779 675
ScaI AGT'ACT
Cuts at: 0 2236 2268
Size: 2236 32
SfcI C'TryA_G
Cuts at: 0 527 2268
Size: 527 1741
SfoI GGC'GCC
Cuts at: 0 282 296 2268
Size: 282 14 1972
Fragments arranged by size:
1972 282 14
SmaI CCC'GGG
Cuts at: 0 78 109 2268
Size: 78 31 2159
Fragments arranged by size:
2159 78 31
SmlI C'TyrA_G
Cuts at: 0 733 856 1636 2268
Size: 733 123 780 632
Fragments arranged by size:
780 733 632 123
SspI AAT'ATT
Cuts at: 0 1118 2268
Size: 1118 1150
StuI AGG'CCT
Cuts at: 0 539 2268
Size: 539 1729
StyI C'CwwG_G
Cuts at: 0 2093 2268
Size: 2093 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 569 2268
Size: 569 1699
TatI w'GTAC_w
Cuts at: 0 143 878 2234 2268
Size: 143 735 1356 34
Fragments arranged by size:
1356 735 143 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 157 189 1088 1120 2244 2268
Size: 157 32 899 32 1124 24
Fragments arranged by size:
1124 899 157 32 32 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2073 2268
Size: 2073 195
XmaI C'CCGG_G
Cuts at: 0 76 107 2268
Size: 76 31 2161
Fragments arranged by size:
2161 76 31
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BfrBI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PpuMI PsiI PspOMI PstI SacI SacII
SalI SapI ScaI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsiWI BspEI BssSI BstBI BstEII
BstZ17I Bsu36I CspCI EciI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI PacI PasI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI PvuII RsrII SanDI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmnI ZraI