Restriction map for
pF1KA1139
(Linear) (Six-base) MAPSORT of: KIAA1139.seq Check: 682 from: 1 to: 3606
KIAA1139.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1183 2082 2766 3606
Size: 1183 899 684 840
Fragments arranged by size:
1183 899 840 684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 3606
Size: 871 2735
AfeI AGC'GCT
Cuts at: 0 863 3606
Size: 863 2743
AgeI A'CCGG_T
Cuts at: 0 49 3606
Size: 49 3557
AleI CACnn'nnGTG
Cuts at: 0 515 3606
Size: 515 3091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 262 380 602 1136 3606
Size: 262 118 222 534 2470
Fragments arranged by size:
2470 534 262 222 118
AlwNI CAG_nnn'CTG
Cuts at: 0 18 63 624 828 1317 1344 1455 2806
Size: 18 45 561 204 489 27 111 1351
Cuts at: 2806 2965 3441 3606
Size: 159 476 165
Fragments arranged by size:
1351 561 489 476 204 165 159 111
45 27 18
ApaI G_GGCC'C
Cuts at: 0 2351 2589 2684 2705 2774 3421 3606
Size: 2351 238 95 21 69 647 185
Fragments arranged by size:
2351 647 238 185 95 69 21
ApaLI G'TGCA_C
Cuts at: 0 1703 3606
Size: 1703 1903
ApoI r'AATT_y
Cuts at: 0 469 3606
Size: 469 3137
AvaI C'yCGr_G
Cuts at: 0 1014 1163 1816 2549 2825 2912 3115 3606
Size: 1014 149 653 733 276 87 203 491
Fragments arranged by size:
1014 733 653 491 276 203 149 87
AvrII C'CTAG_G
Cuts at: 0 2466 3606
Size: 2466 1140
BamHI G'GATC_C
Cuts at: 0 845 3606
Size: 845 2761
BanI G'GyrC_C
Cuts at: 0 1907 3282 3499 3517 3606
Size: 1907 1375 217 18 89
Fragments arranged by size:
1907 1375 217 89 18
BanII G_rGCy'C
Cuts at: 0 106 191 1264 2284 2351 2589 2677 2684
Size: 106 85 1073 1020 67 238 88 7
Cuts at: 2684 2705 2747 2774 3122 3421 3606
Size: 21 42 27 348 299 185
Fragments arranged by size:
1073 1020 348 299 238 185 106 88
85 67 42 27 21 7
BbeI G_GCGC'C
Cuts at: 0 1911 3606
Size: 1911 1695
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 3606
Size: 451 3155
BbvCI CC'TCA_GC
Cuts at: 0 1174 1894 2432 2626 3606
Size: 1174 720 538 194 980
Fragments arranged by size:
1174 980 720 538 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 911 3549 3583 3606
Size: 877 34 2638 34 23
Fragments arranged by size:
2638 877 34 34 23
BglI GCCn_nnn'nGGC
Cuts at: 0 294 316 986 2411 2584 2709 3606
Size: 294 22 670 1425 173 125 897
Fragments arranged by size:
1425 897 670 294 173 125 22
BglII A'GATC_T
Cuts at: 0 1421 3606
Size: 1421 2185
BlpI GC'TnA_GC
Cuts at: 0 1492 1640 1645 3219 3606
Size: 1492 148 5 1574 387
Fragments arranged by size:
1574 1492 387 148 5
Bme1580I G_kGCm'C
Cuts at: 0 991 1273 1613 1707 2109 2206 2351 2511
Size: 991 282 340 94 402 97 145 160
Cuts at: 2511 2589 2684 2705 2774 2930 3287 3421 3520
Size: 78 95 21 69 156 357 134 99
Cuts at: 3520 3606
Size: 86
Fragments arranged by size:
991 402 357 340 282 160 156 145
134 99 97 95 94 86 78 69
21
BmgBI CAC'GTC
Cuts at: 0 738 1437 3606
Size: 738 699 2169
Fragments arranged by size:
2169 738 699
BmrI ACTGGGnnnn_n'
Cuts at: 0 1717 3373 3416 3606
Size: 1717 1656 43 190
Fragments arranged by size:
1717 1656 190 43
BmtI G_CTAG'C
Cuts at: 0 3358 3606
Size: 3358 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 304 388 550 742 1349 1477 1528
Size: 205 99 84 162 192 607 128 51
Cuts at: 1528 1711 1972 3077 3167 3463 3606
Size: 183 261 1105 90 296 143
Fragments arranged by size:
1105 607 296 261 205 192 183 162
143 128 99 90 84 51
Bpu10I CC'TnA_GC
Cuts at: 0 212 1174 1381 1894 2219 2381 2401 2432
Size: 212 962 207 513 325 162 20 31
Cuts at: 2432 2626 3350 3606
Size: 194 724 256
Fragments arranged by size:
962 724 513 325 256 212 207 194
162 31 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 850 3262 3606
Size: 466 384 2412 344
Fragments arranged by size:
2412 466 384 344
BsaAI yAC'GTr
Cuts at: 0 755 3606
Size: 755 2851
BsaBI GATnn'nnATC
Cuts at: 0 640 1939 3606
Size: 640 1299 1667
Fragments arranged by size:
1667 1299 640
BsaHI Gr'CG_yC
Cuts at: 0 448 1908 2815 3572 3593 3606
Size: 448 1460 907 757 21 13
Fragments arranged by size:
1460 907 757 448 21 13
BsaWI w'CCGG_w
Cuts at: 0 49 746 1926 2516 3606
Size: 49 697 1180 590 1090
Fragments arranged by size:
1180 1090 697 590 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1684 1714 2051 2081 3606
Size: 1684 30 337 30 1525
Fragments arranged by size:
1684 1525 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1801 1981 2549 2803 2806 2809 3080
Size: 391 1410 180 568 254 3 3 271
Cuts at: 3080 3265 3377 3606
Size: 185 112 229
Fragments arranged by size:
1410 568 391 271 254 229 185 180
112 3 3
BseYI C'CCAG_C
Cuts at: 0 491 631 1188 1310 1666 1839 1968 2086
Size: 491 140 557 122 356 173 129 118
Cuts at: 2086 2155 2170 2544 2644 2767 3110 3121 3163
Size: 69 15 374 100 123 343 11 42
Cuts at: 3163 3329 3431 3523 3606
Size: 166 102 92 83
Fragments arranged by size:
557 491 374 356 343 173 166 140
129 123 122 118 102 100 92 83
69 42 15 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 241 340 350 562 3309 3455 3606
Size: 76 165 99 10 212 2747 146 151
Fragments arranged by size:
2747 212 165 151 146 99 76 10
BsiEI CG_ry'CG
Cuts at: 0 954 3260 3606
Size: 954 2306 346
Fragments arranged by size:
2306 954 346
BsiHKAI G_wGCw'C
Cuts at: 0 191 1264 1707 3406 3606
Size: 191 1073 443 1699 200
Fragments arranged by size:
1699 1073 443 200 191
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2640 3606
Size: 2640 966
Bsp1286I G_dGCh'C
Cuts at: 0 106 191 991 1264 1273 1613 1707 2109
Size: 106 85 800 273 9 340 94 402
Cuts at: 2109 2206 2284 2351 2511 2589 2677 2684 2705
Size: 97 78 67 160 78 88 7 21
Cuts at: 2705 2747 2774 2930 3122 3287 3406 3421 3520
Size: 42 27 156 192 165 119 15 99
Cuts at: 3520 3606
Size: 86
Fragments arranged by size:
800 402 340 273 192 165 160 156
119 106 99 97 94 88 86 85
78 78 67 42 27 21 15 9
7
BspEI T'CCGG_A
Cuts at: 0 746 1926 2516 3606
Size: 746 1180 590 1090
Fragments arranged by size:
1180 1090 746 590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1183 1694 2082 2736 2766 3606
Size: 1183 511 388 654 30 840
Fragments arranged by size:
1183 840 654 511 388 30
BsrBI CCG'CTC
Cuts at: 0 1409 2081 2211 3606
Size: 1409 672 130 1395
Fragments arranged by size:
1409 1395 672 130
BsrDI GCAATG_nn'
Cuts at: 0 246 3606
Size: 246 3360
BsrFI r'CCGG_y
Cuts at: 0 49 278 1246 1537 1910 2387 3606
Size: 49 229 968 291 373 477 1219
Fragments arranged by size:
1219 968 477 373 291 229 49
BssHII G'CGCG_C
Cuts at: 0 306 3606
Size: 306 3300
BssSI C'ACGA_G
Cuts at: 0 676 973 3606
Size: 676 297 2633
Fragments arranged by size:
2633 676 297
BstAPI GCAn_nnn'nTGC
Cuts at: 0 828 3606
Size: 828 2778
BstEII G'GTnAC_C
Cuts at: 0 1596 3606
Size: 1596 2010
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1288 1764 3606
Size: 1288 476 1842
Fragments arranged by size:
1842 1288 476
BstYI r'GATC_y
Cuts at: 0 347 845 1421 2166 3606
Size: 347 498 576 745 1440
Fragments arranged by size:
1440 745 576 498 347
Bsu36I CC'TnA_GG
Cuts at: 0 840 1574 2807 3606
Size: 840 734 1233 799
Fragments arranged by size:
1233 840 799 734
BtgI C'CryG_G
Cuts at: 0 1281 1757 1984 2036 3391 3606
Size: 1281 476 227 52 1355 215
Fragments arranged by size:
1355 1281 476 227 215 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1635 3606
Size: 1635 1971
BtsI GCAGTG_nn'
Cuts at: 0 523 893 1221 1248 1315 1517 2511 3001
Size: 523 370 328 27 67 202 994 490
Cuts at: 3001 3013 3061 3606
Size: 12 48 545
Fragments arranged by size:
994 545 523 490 370 328 202 67
48 27 12
DraIII CAC_nnn'GTG
Cuts at: 0 2722 3606
Size: 2722 884
DrdI GACnn_nn'nnGTC
Cuts at: 0 3456 3606
Size: 3456 150
EaeI y'GGCC_r
Cuts at: 0 474 597 951 1278 1397 1441 1586 1769
Size: 474 123 354 327 119 44 145 183
Cuts at: 1769 1916 1937 2033 2124 2241 2342 2494 3032
Size: 147 21 96 91 117 101 152 538
Cuts at: 3032 3257 3606
Size: 225 349
Fragments arranged by size:
538 474 354 349 327 225 183 152
147 145 123 119 117 101 96 91
44 21
EagI C'GGCC_G
Cuts at: 0 951 3257 3606
Size: 951 2306 349
Fragments arranged by size:
2306 951 349
EarI CTCTTCn'nnn_
Cuts at: 0 3484 3606
Size: 3484 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1873 3606
Size: 1873 1733
EcoICRI GAG'CTC
Cuts at: 0 189 1262 3606
Size: 189 1073 2344
Fragments arranged by size:
2344 1073 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 304 388 550 742 871 1349 1477
Size: 205 99 84 162 192 129 478 128
Cuts at: 1477 1528 1711 1972 3077 3167 3463 3606
Size: 51 183 261 1105 90 296 143
Fragments arranged by size:
1105 478 296 261 205 192 183 162
143 129 128 99 90 84 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1879 2197 2243 3277 3606
Size: 1879 318 46 1034 329
Fragments arranged by size:
1879 1034 329 318 46
EcoO109I rG'GnC_Cy
Cuts at: 0 15 1578 1872 1890 2248 2348 2599 2680
Size: 15 1563 294 18 358 100 251 81
Cuts at: 2680 2701 2702 2803 3606
Size: 21 1 101 803
Fragments arranged by size:
1563 803 358 294 251 101 100 81
21 18 15 1
HaeII r_GCGC'y
Cuts at: 0 673 772 865 1911 2388 2625 2889 3361
Size: 673 99 93 1046 477 237 264 472
Cuts at: 3361 3530 3606
Size: 169 76
Fragments arranged by size:
1046 673 477 472 264 237 169 99
93 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 596 620 628 652 3606
Size: 133 32 431 24 8 24 2954
Fragments arranged by size:
2954 431 133 32 24 24 8
HincII GTy'rAC
Cuts at: 0 225 432 1809 3606
Size: 225 207 1377 1797
Fragments arranged by size:
1797 1377 225 207
HindIII A'AGCT_T
Cuts at: 0 2221 3606
Size: 2221 1385
KasI G'GCGC_C
Cuts at: 0 1907 3606
Size: 1907 1699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 3172 3606
Size: 440 2732 434
Fragments arranged by size:
2732 440 434
MscI TGG'CCA
Cuts at: 0 599 1280 1399 1918 1939 2126 2243 3034
Size: 599 681 119 519 21 187 117 791
Cuts at: 3034 3606
Size: 572
Fragments arranged by size:
791 681 599 572 519 187 119 117
21
MslI CAynn'nnrTG
Cuts at: 0 515 928 1594 3606
Size: 515 413 666 2012
Fragments arranged by size:
2012 666 515 413
MspA1I CmG'CkG
Cuts at: 0 255 495 956 1253 1452 1506 1734 1745
Size: 255 240 461 297 199 54 228 11
Cuts at: 1745 1978 2159 2899 3055 3333 3469 3588 3606
Size: 233 181 740 156 278 136 119 18
Fragments arranged by size:
740 461 297 278 255 240 233 228
199 181 156 136 119 54 18 11
NaeI GCC'GGC
Cuts at: 0 280 1248 1539 1912 3606
Size: 280 968 291 373 1694
Fragments arranged by size:
1694 968 373 291 280
NarI GG'CG_CC
Cuts at: 0 1908 3606
Size: 1908 1698
NcoI C'CATG_G
Cuts at: 0 1281 1757 2036 3606
Size: 1281 476 279 1570
Fragments arranged by size:
1570 1281 476 279
NgoMIV G'CCGG_C
Cuts at: 0 278 1246 1537 1910 3606
Size: 278 968 291 373 1696
Fragments arranged by size:
1696 968 373 291 278
NheI G'CTAG_C
Cuts at: 0 3354 3606
Size: 3354 252
NspI r_CATG'y
Cuts at: 0 249 3606
Size: 249 3357
PasI CC'CwG_GG
Cuts at: 0 2277 2302 2324 2677 3199 3606
Size: 2277 25 22 353 522 407
Fragments arranged by size:
2277 522 407 353 25 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 2314 2668 3079 3606
Size: 2314 354 411 527
Fragments arranged by size:
2314 527 411 354
PfoI T'CCnGG_A
Cuts at: 0 1102 3606
Size: 1102 2504
PpuMI rG'GwC_Cy
Cuts at: 0 15 1578 2248 3606
Size: 15 1563 670 1358
Fragments arranged by size:
1563 1358 670 15
PspOMI G'GGCC_C
Cuts at: 0 2347 2585 2680 2701 2770 3417 3606
Size: 2347 238 95 21 69 647 189
Fragments arranged by size:
2347 647 238 189 95 69 21
PstI C_TGCA'G
Cuts at: 0 1059 1535 1796 1883 3008 3606
Size: 1059 476 261 87 1125 598
Fragments arranged by size:
1125 1059 598 476 261 87
PvuII CAG'CTG
Cuts at: 0 495 1452 1506 1734 1978 3333 3469 3588
Size: 495 957 54 228 244 1355 136 119
Cuts at: 3588 3606
Size: 18
Fragments arranged by size:
1355 957 495 244 228 136 119 54
18
SacI G_AGCT'C
Cuts at: 0 191 1264 3606
Size: 191 1073 2342
Fragments arranged by size:
2342 1073 191
SapI GCTCTTCn'nnn_
Cuts at: 0 3484 3606
Size: 3484 122
SexAI A'CCwGG_T
Cuts at: 0 1337 3606
Size: 1337 2269
SfcI C'TryA_G
Cuts at: 0 1055 1149 1531 1792 1879 1973 2026 3004
Size: 1055 94 382 261 87 94 53 978
Cuts at: 3004 3606
Size: 602
Fragments arranged by size:
1055 978 602 382 261 94 94 87
53
SfoI GGC'GCC
Cuts at: 0 1909 3606
Size: 1909 1697
SmaI CCC'GGG
Cuts at: 0 1016 3606
Size: 1016 2590
SmlI C'TyrA_G
Cuts at: 0 481 865 3277 3606
Size: 481 384 2412 329
Fragments arranged by size:
2412 481 384 329
SnaBI TAC'GTA
Cuts at: 0 755 3606
Size: 755 2851
SphI G_CATG'C
Cuts at: 0 249 3606
Size: 249 3357
StuI AGG'CCT
Cuts at: 0 838 3606
Size: 838 2768
StyI C'CwwG_G
Cuts at: 0 1281 1757 1919 2036 2244 2466 2684 3138
Size: 1281 476 162 117 208 222 218 454
Cuts at: 3138 3503 3606
Size: 365 103
Fragments arranged by size:
1281 476 454 365 222 218 208 162
117 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 715 1092 1444 3343 3606
Size: 715 377 352 1899 263
Fragments arranged by size:
1899 715 377 352 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1348 1380 1684 1716 2051 2083 3606
Size: 1348 32 304 32 335 32 1523
Fragments arranged by size:
1523 1348 335 304 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 139 423 1764 1947 3459 3543 3606
Size: 139 284 1341 183 1512 84 63
Fragments arranged by size:
1512 1341 284 183 139 84 63
XmaI C'CCGG_G
Cuts at: 0 1014 3606
Size: 1014 2592
XmnI GAAnn'nnTTC
Cuts at: 0 787 3606
Size: 787 2819
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SapI SexAI SfcI SfoI SmaI SmlI SnaBI
SphI StuI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AloI
AscI AseI AsiSI BaeI BciVI BclI BfrBI BplI
BsaI BsiWI BsmI BspHI BsrGI BstBI BstZ17I ClaI
CspCI DraI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KpnI MfeI MluI NdeI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI ZraI