Restriction map for
pF1KA1140
(Linear) (Six-base) MAPSORT of: KIAA1140.seq Check: 337 from: 1 to: 2217
KIAA1140.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1361 1464 2217
Size: 1361 103 753
Fragments arranged by size:
1361 753 103
Acc65I G'GTAC_C
Cuts at: 0 893 2217
Size: 893 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1180 1339 1759 2217
Size: 418 762 159 420 458
Fragments arranged by size:
762 458 420 418 159
AflIII A'CryG_T
Cuts at: 0 37 2217
Size: 37 2180
AhdI GACnn_n'nnGTC
Cuts at: 0 829 2217
Size: 829 1388
AleI CACnn'nnGTG
Cuts at: 0 1459 2217
Size: 1459 758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1534 1854 2217
Size: 1534 320 363
Fragments arranged by size:
1534 363 320
AlwNI CAG_nnn'CTG
Cuts at: 0 612 1203 1221 1479 1539 1657 2097 2217
Size: 612 591 18 258 60 118 440 120
Fragments arranged by size:
612 591 440 258 120 118 60 18
ApaI G_GGCC'C
Cuts at: 0 1750 2217
Size: 1750 467
ApoI r'AATT_y
Cuts at: 0 880 2217
Size: 880 1337
AvaI C'yCGr_G
Cuts at: 0 713 2217
Size: 713 1504
BanI G'GyrC_C
Cuts at: 0 735 893 1224 2217
Size: 735 158 331 993
Fragments arranged by size:
993 735 331 158
BanII G_rGCy'C
Cuts at: 0 350 434 550 1215 1750 2216 2217
Size: 350 84 116 665 535 466 1
Fragments arranged by size:
665 535 466 350 116 84 1
BbeI G_GCGC'C
Cuts at: 0 1228 2217
Size: 1228 989
BbsI GAAGACnn'nnnn_
Cuts at: 0 295 2217
Size: 295 1922
BbvCI CC'TCA_GC
Cuts at: 0 1522 1907 2217
Size: 1522 385 310
Fragments arranged by size:
1522 385 310
BclI T'GATC_A
Cuts at: 0 230 1067 2217
Size: 230 837 1150
Fragments arranged by size:
1150 837 230
BfrBI ATG'CAT
Cuts at: 0 1989 2217
Size: 1989 228
BglI GCCn_nnn'nGGC
Cuts at: 0 607 2217
Size: 607 1610
BglII A'GATC_T
Cuts at: 0 1283 1778 2217
Size: 1283 495 439
Fragments arranged by size:
1283 495 439
BlpI GC'TnA_GC
Cuts at: 0 727 1214 1796 2217
Size: 727 487 582 421
Fragments arranged by size:
727 582 487 421
Bme1580I G_kGCm'C
Cuts at: 0 995 1750 2217
Size: 995 755 467
Fragments arranged by size:
995 755 467
BmgBI CAC'GTC
Cuts at: 0 308 2217
Size: 308 1909
BmrI ACTGGGnnnn_n'
Cuts at: 0 1223 2217
Size: 1223 994
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 267 661 2217
Size: 267 394 1556
Fragments arranged by size:
1556 394 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 564 871 1225 1272 1489 1717 1945
Size: 358 206 307 354 47 217 228 228
Cuts at: 1945 2188 2217
Size: 243 29
Fragments arranged by size:
358 354 307 243 228 228 217 206
47 29
Bpu10I CC'TnA_GC
Cuts at: 0 263 799 1522 1907 2217
Size: 263 536 723 385 310
Fragments arranged by size:
723 536 385 310 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 2182 2217
Size: 1807 375 35
Fragments arranged by size:
1807 375 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 1129 2217
Size: 451 678 1088
Fragments arranged by size:
1088 678 451
BsaAI yAC'GTr
Cuts at: 0 2075 2217
Size: 2075 142
BsaBI GATnn'nnATC
Cuts at: 0 1066 2217
Size: 1066 1151
BsaHI Gr'CG_yC
Cuts at: 0 1151 1225 2217
Size: 1151 74 992
Fragments arranged by size:
1151 992 74
BsaWI w'CCGG_w
Cuts at: 0 755 1184 2217
Size: 755 429 1033
Fragments arranged by size:
1033 755 429
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 1645 1675 1693 1723 2217
Size: 1534 30 81 30 18 30 494
Fragments arranged by size:
1534 494 81 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 547 601 676 679 763 1714 1942
Size: 235 312 54 75 3 84 951 228
Cuts at: 1942 2217
Size: 275
Fragments arranged by size:
951 312 275 235 228 84 75 54
3
BseYI C'CCAG_C
Cuts at: 0 1553 1557 2016 2217
Size: 1553 4 459 201
Fragments arranged by size:
1553 459 201 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 2217
Size: 502 1715
BsiHKAI G_wGCw'C
Cuts at: 0 350 434 1052 1430 2216 2217
Size: 350 84 618 378 786 1
Fragments arranged by size:
786 618 378 350 84 1
BsmI GAATG_Cn'
Cuts at: 0 780 2217
Size: 780 1437
Bsp1286I G_dGCh'C
Cuts at: 0 350 434 550 995 1052 1215 1430 1750
Size: 350 84 116 445 57 163 215 320
Cuts at: 1750 2216 2217
Size: 466 1
Fragments arranged by size:
466 445 350 320 215 163 116 84
57 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1361 1464 1516 1822 2217
Size: 1361 103 52 306 395
Fragments arranged by size:
1361 395 306 103 52
BsrFI r'CCGG_y
Cuts at: 0 1898 2217
Size: 1898 319
BsrGI T'GTAC_A
Cuts at: 0 1541 1943 2217
Size: 1541 402 274
Fragments arranged by size:
1541 402 274
BssSI C'ACGA_G
Cuts at: 0 1501 2080 2217
Size: 1501 579 137
Fragments arranged by size:
1501 579 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 1296 2217
Size: 501 795 921
Fragments arranged by size:
921 795 501
BstYI r'GATC_y
Cuts at: 0 1283 1778 2217
Size: 1283 495 439
Fragments arranged by size:
1283 495 439
BtgI C'CryG_G
Cuts at: 0 43 704 911 1295 2057 2217
Size: 43 661 207 384 762 160
Fragments arranged by size:
762 661 384 207 160 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 174 1665 2217
Size: 174 1491 552
Fragments arranged by size:
1491 552 174
BtsI GCAGTG_nn'
Cuts at: 0 1192 1360 2217
Size: 1192 168 857
Fragments arranged by size:
1192 857 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 1240 2217
Size: 1240 977
EaeI y'GGCC_r
Cuts at: 0 491 1001 1415 2217
Size: 491 510 414 802
Fragments arranged by size:
802 510 491 414
EarI CTCTTCn'nnn_
Cuts at: 0 1372 1734 1867 2217
Size: 1372 362 133 350
Fragments arranged by size:
1372 362 350 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 193 2217
Size: 193 2024
EcoICRI GAG'CTC
Cuts at: 0 348 432 2214 2217
Size: 348 84 1782 3
Fragments arranged by size:
1782 348 84 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 418 564 871 1180 1225 1272 1339
Size: 358 60 146 307 309 45 47 67
Cuts at: 1339 1489 1717 1759 1945 2188 2217
Size: 150 228 42 186 243 29
Fragments arranged by size:
358 309 307 243 228 186 150 146
67 60 47 45 42 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 529 1078 1102 1570 1741 1927 2101
Size: 400 129 549 24 468 171 186 174
Cuts at: 2101 2217
Size: 116
Fragments arranged by size:
549 468 400 186 174 171 129 116
24
EcoO109I rG'GnC_Cy
Cuts at: 0 146 248 510 609 1022 1314 1593 1746
Size: 146 102 262 99 413 292 279 153
Cuts at: 1746 1747 2094 2217
Size: 1 347 123
Fragments arranged by size:
413 347 292 279 262 153 146 123
102 99 1
FspI TGC'GCA
Cuts at: 0 1981 2217
Size: 1981 236
FspAI rTGC'GCAy
Cuts at: 0 1981 2217
Size: 1981 236
HaeII r_GCGC'y
Cuts at: 0 383 1228 1957 2217
Size: 383 845 729 260
Fragments arranged by size:
845 729 383 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 815 847 1639 1645 1671 1677
Size: 24 32 759 32 792 6 26 6
Cuts at: 1677 1693 1725 1995 2027 2217
Size: 16 32 270 32 190
Fragments arranged by size:
792 759 270 190 32 32 32 32
26 24 16 6 6
HincII GTy'rAC
Cuts at: 0 1410 2142 2217
Size: 1410 732 75
Fragments arranged by size:
1410 732 75
KasI G'GCGC_C
Cuts at: 0 1224 2217
Size: 1224 993
KpnI G_GTAC'C
Cuts at: 0 897 2217
Size: 897 1320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1302 2217
Size: 61 1241 915
Fragments arranged by size:
1241 915 61
MscI TGG'CCA
Cuts at: 0 1417 2217
Size: 1417 800
MslI CAynn'nnrTG
Cuts at: 0 9 42 629 1063 1391 1459 2217
Size: 9 33 587 434 328 68 758
Fragments arranged by size:
758 587 434 328 68 33 9
MspA1I CmG'CkG
Cuts at: 0 1221 1266 1305 1557 1758 1941 2217
Size: 1221 45 39 252 201 183 276
Fragments arranged by size:
1221 276 252 201 183 45 39
NaeI GCC'GGC
Cuts at: 0 1900 2217
Size: 1900 317
NarI GG'CG_CC
Cuts at: 0 1225 2217
Size: 1225 992
NcoI C'CATG_G
Cuts at: 0 43 704 911 1295 2217
Size: 43 661 207 384 922
Fragments arranged by size:
922 661 384 207 43
NgoMIV G'CCGG_C
Cuts at: 0 1898 2217
Size: 1898 319
NsiI A_TGCA'T
Cuts at: 0 1991 2217
Size: 1991 226
NspI r_CATG'y
Cuts at: 0 14 41 106 1396 1623 2217
Size: 14 27 65 1290 227 594
Fragments arranged by size:
1290 594 227 65 27 14
PasI CC'CwG_GG
Cuts at: 0 2118 2217
Size: 2118 99
PciI A'CATG_T
Cuts at: 0 37 2217
Size: 37 2180
PflMI CCAn_nnn'nTGG
Cuts at: 0 1356 2217
Size: 1356 861
PfoI T'CCnGG_A
Cuts at: 0 253 359 434 1844 2217
Size: 253 106 75 1410 373
Fragments arranged by size:
1410 373 253 106 75
PpuMI rG'GwC_Cy
Cuts at: 0 146 510 1022 2217
Size: 146 364 512 1195
Fragments arranged by size:
1195 512 364 146
PspOMI G'GGCC_C
Cuts at: 0 1746 2217
Size: 1746 471
PstI C_TGCA'G
Cuts at: 0 154 278 1142 1199 1265 1310 1513 1790
Size: 154 124 864 57 66 45 203 277
Cuts at: 1790 2114 2217
Size: 324 103
Fragments arranged by size:
864 324 277 203 154 124 103 66
57 45
PvuII CAG'CTG
Cuts at: 0 1221 1266 1305 1557 1758 1941 2217
Size: 1221 45 39 252 201 183 276
Fragments arranged by size:
1221 276 252 201 183 45 39
SacI G_AGCT'C
Cuts at: 0 350 434 2216 2217
Size: 350 84 1782 1
Fragments arranged by size:
1782 350 84 1
SanDI GG'GwC_CC
Cuts at: 0 1022 2217
Size: 1022 1195
SapI GCTCTTCn'nnn_
Cuts at: 0 1372 2217
Size: 1372 845
SbfI CC_TGCA'GG
Cuts at: 0 154 278 1142 2217
Size: 154 124 864 1075
Fragments arranged by size:
1075 864 154 124
ScaI AGT'ACT
Cuts at: 0 1883 2217
Size: 1883 334
SexAI A'CCwGG_T
Cuts at: 0 896 2217
Size: 896 1321
SfcI C'TryA_G
Cuts at: 0 150 274 1131 1138 1195 1261 1306 1509
Size: 150 124 857 7 57 66 45 203
Cuts at: 1509 1786 2110 2217
Size: 277 324 107
Fragments arranged by size:
857 324 277 203 150 124 107 66
57 45 7
SfoI GGC'GCC
Cuts at: 0 1226 2217
Size: 1226 991
SmlI C'TyrA_G
Cuts at: 0 713 1822 2161 2217
Size: 713 1109 339 56
Fragments arranged by size:
1109 713 339 56
SphI G_CATG'C
Cuts at: 0 106 1396 1623 2217
Size: 106 1290 227 594
Fragments arranged by size:
1290 594 227 106
StuI AGG'CCT
Cuts at: 0 161 1565 2217
Size: 161 1404 652
Fragments arranged by size:
1404 652 161
StyI C'CwwG_G
Cuts at: 0 43 656 704 911 1103 1295 1457 2217
Size: 43 613 48 207 192 192 162 760
Fragments arranged by size:
760 613 207 192 192 162 48 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1722 2217
Size: 1722 495
TatI w'GTAC_w
Cuts at: 0 1541 1881 1943 2184 2217
Size: 1541 340 62 241 33
Fragments arranged by size:
1541 340 241 62 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 1464 2217
Size: 1110 354 753
Fragments arranged by size:
1110 753 354
XhoI C'TCGA_G
Cuts at: 0 713 2217
Size: 713 1504
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI
BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI FspAI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BmtI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII EagI EcoRI EcoRV FalI FseI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XmaI
XmnI ZraI