Restriction map for
pF1KA1154
(Linear) (Six-base) MAPSORT of: KIAA1154.seq Check: 6172 from: 1 to: 1428
KIAA1154.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 1040 1334 1428
Size: 1040 294 94
Fragments arranged by size:
1040 294 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 388 841 1428
Size: 163 225 453 587
Fragments arranged by size:
587 453 225 163
AflIII A'CryG_T
Cuts at: 0 377 1428
Size: 377 1051
AlwNI CAG_nnn'CTG
Cuts at: 0 819 1428
Size: 819 609
ApaLI G'TGCA_C
Cuts at: 0 414 1428
Size: 414 1014
ApoI r'AATT_y
Cuts at: 0 849 894 1428
Size: 849 45 534
Fragments arranged by size:
849 534 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 986 1019 1428
Size: 986 33 409
Fragments arranged by size:
986 409 33
BamHI G'GATC_C
Cuts at: 0 736 790 1428
Size: 736 54 638
Fragments arranged by size:
736 638 54
BanI G'GyrC_C
Cuts at: 0 92 168 1219 1428
Size: 92 76 1051 209
Fragments arranged by size:
1051 209 92 76
BbeI G_GCGC'C
Cuts at: 0 96 1428
Size: 96 1332
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 848 1097 1428
Size: 129 719 249 331
Fragments arranged by size:
719 331 249 129
BbvCI CC'TCA_GC
Cuts at: 0 812 1428
Size: 812 616
Bme1580I G_kGCm'C
Cuts at: 0 418 1428
Size: 418 1010
BmgBI CAC'GTC
Cuts at: 0 846 1428
Size: 846 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 962 1428
Size: 284 678 466
Fragments arranged by size:
678 466 284
Bpu10I CC'TnA_GC
Cuts at: 0 812 1154 1428
Size: 812 342 274
Fragments arranged by size:
812 342 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 1428
Size: 1283 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 1428
Size: 434 994
BsaAI yAC'GTr
Cuts at: 0 258 1428
Size: 258 1170
BsaHI Gr'CG_yC
Cuts at: 0 93 1428
Size: 93 1335
BsaWI w'CCGG_w
Cuts at: 0 221 1428
Size: 221 1207
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 552 800 1180 1243 1255 1428
Size: 552 248 380 63 12 173
Fragments arranged by size:
552 380 248 173 63 12
BseYI C'CCAG_C
Cuts at: 0 453 1428
Size: 453 975
BsiHKAI G_wGCw'C
Cuts at: 0 418 1428
Size: 418 1010
Bsp1286I G_dGCh'C
Cuts at: 0 418 1428
Size: 418 1010
BspEI T'CCGG_A
Cuts at: 0 221 1428
Size: 221 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1242 1428
Size: 1242 186
BsrBI CCG'CTC
Cuts at: 0 5 245 542 1428
Size: 5 240 297 886
Fragments arranged by size:
886 297 240 5
BsrFI r'CCGG_y
Cuts at: 0 154 1428
Size: 154 1274
BssSI C'ACGA_G
Cuts at: 0 1205 1428
Size: 1205 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1201 1428
Size: 1201 227
BstBI TT'CG_AA
Cuts at: 0 131 1428
Size: 131 1297
BstEII G'GTnAC_C
Cuts at: 0 150 1111 1428
Size: 150 961 317
Fragments arranged by size:
961 317 150
BstYI r'GATC_y
Cuts at: 0 236 736 790 1428
Size: 236 500 54 638
Fragments arranged by size:
638 500 236 54
BtgI C'CryG_G
Cuts at: 0 202 1421 1428
Size: 202 1219 7
Fragments arranged by size:
1219 202 7
BtsI GCAGTG_nn'
Cuts at: 0 1317 1428
Size: 1317 111
EaeI y'GGCC_r
Cuts at: 0 210 1428
Size: 210 1218
EarI CTCTTCn'nnn_
Cuts at: 0 43 817 1115 1428
Size: 43 774 298 313
Fragments arranged by size:
774 313 298 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 284 388 841 962 1428
Size: 163 121 104 453 121 466
Fragments arranged by size:
466 453 163 121 121 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 731 1120 1282 1428
Size: 145 586 389 162 146
Fragments arranged by size:
586 389 162 146 145
EcoO109I rG'GnC_Cy
Cuts at: 0 75 1428
Size: 75 1353
EcoRI G'AATT_C
Cuts at: 0 849 894 1428
Size: 849 45 534
Fragments arranged by size:
849 534 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 595 1428
Size: 595 833
HaeII r_GCGC'y
Cuts at: 0 17 96 1428
Size: 17 79 1332
Fragments arranged by size:
1332 79 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 790 822 1161 1193 1428
Size: 757 32 1 32 339 32 235
Fragments arranged by size:
757 339 235 32 32 32 1
HincII GTy'rAC
Cuts at: 0 683 782 1428
Size: 683 99 646
Fragments arranged by size:
683 646 99
HindIII A'AGCT_T
Cuts at: 0 710 1428
Size: 710 718
KasI G'GCGC_C
Cuts at: 0 92 1428
Size: 92 1336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 768 1428
Size: 581 187 660
Fragments arranged by size:
660 581 187
MscI TGG'CCA
Cuts at: 0 212 1428
Size: 212 1216
MspA1I CmG'CkG
Cuts at: 0 204 1428
Size: 204 1224
NarI GG'CG_CC
Cuts at: 0 93 1428
Size: 93 1335
PpuMI rG'GwC_Cy
Cuts at: 0 75 1428
Size: 75 1353
PstI C_TGCA'G
Cuts at: 0 1250 1428
Size: 1250 178
SacII CC_GC'GG
Cuts at: 0 205 1428
Size: 205 1223
SanDI GG'GwC_CC
Cuts at: 0 75 1428
Size: 75 1353
SapI GCTCTTCn'nnn_
Cuts at: 0 43 1428
Size: 43 1385
SfcI C'TryA_G
Cuts at: 0 1142 1246 1428
Size: 1142 104 182
Fragments arranged by size:
1142 182 104
SfoI GGC'GCC
Cuts at: 0 94 1428
Size: 94 1334
SmlI C'TyrA_G
Cuts at: 0 1298 1428
Size: 1298 130
StuI AGG'CCT
Cuts at: 0 696 1428
Size: 696 732
StyI C'CwwG_G
Cuts at: 0 1351 1428
Size: 1351 77
TatI w'GTAC_w
Cuts at: 0 504 1428
Size: 504 924
XmnI GAAnn'nnTTC
Cuts at: 0 908 1428
Size: 908 520
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI BaeI BamHI
BanI BbeI BbsI BbvCI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI
MmeI MscI MspA1I NarI PpuMI PstI SacII SanDI
SapI SfcI SfoI SmlI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BanII BcgI BciVI BclI BfrBI BglI BglII
BlpI BmrI BmtI BplI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HpaI
KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI ZraI