Restriction map for
pF1KSDA1158
(Linear) (Six-base) MAPSORT of: KIAA1158.seq Check: 911 from: 1 to: 645
KIAA1158.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 364 645
Size: 364 281
Acc65I G'GTAC_C
Cuts at: 0 630 645
Size: 630 15
AflIII A'CryG_T
Cuts at: 0 305 581 645
Size: 305 276 64
Fragments arranged by size:
305 276 64
AleI CACnn'nnGTG
Cuts at: 0 172 175 463 645
Size: 172 3 288 182
Fragments arranged by size:
288 182 172 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 645
Size: 313 332
AlwNI CAG_nnn'CTG
Cuts at: 0 62 645
Size: 62 583
AvaI C'yCGr_G
Cuts at: 0 196 368 645
Size: 196 172 277
Fragments arranged by size:
277 196 172
BanI G'GyrC_C
Cuts at: 0 269 630 645
Size: 269 361 15
Fragments arranged by size:
361 269 15
BanII G_rGCy'C
Cuts at: 0 259 645
Size: 259 386
BbeI G_GCGC'C
Cuts at: 0 273 645
Size: 273 372
BbsI GAAGACnn'nnnn_
Cuts at: 0 412 492 645
Size: 412 80 153
Fragments arranged by size:
412 153 80
BglI GCCn_nnn'nGGC
Cuts at: 0 565 645
Size: 565 80
Bme1580I G_kGCm'C
Cuts at: 0 89 645
Size: 89 556
BmgBI CAC'GTC
Cuts at: 0 306 645
Size: 306 339
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 645
Size: 60 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 645
Size: 467 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 474 555 645
Size: 474 81 90
Fragments arranged by size:
474 90 81
BsaBI GATnn'nnATC
Cuts at: 0 487 645
Size: 487 158
BsaHI Gr'CG_yC
Cuts at: 0 270 645
Size: 270 375
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 645
Size: 364 30 251
Fragments arranged by size:
364 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 454 645
Size: 169 285 191
Fragments arranged by size:
285 191 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 645
Size: 127 518
BsiHKAI G_wGCw'C
Cuts at: 0 323 645
Size: 323 322
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 645
Size: 88 557
Bsp1286I G_dGCh'C
Cuts at: 0 89 259 323 645
Size: 89 170 64 322
Fragments arranged by size:
322 170 89 64
BspHI T'CATG_A
Cuts at: 0 479 645
Size: 479 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 364 645
Size: 305 59 281
Fragments arranged by size:
305 281 59
BsrDI GCAATG_nn'
Cuts at: 0 366 645
Size: 366 279
BsrGI T'GTAC_A
Cuts at: 0 485 645
Size: 485 160
BssSI C'ACGA_G
Cuts at: 0 37 645
Size: 37 608
BstAPI GCAn_nnn'nTGC
Cuts at: 0 140 645
Size: 140 505
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 177 645
Size: 174 3 468
Fragments arranged by size:
468 174 3
BtgI C'CryG_G
Cuts at: 0 170 645
Size: 170 475
BtsI GCAGTG_nn'
Cuts at: 0 283 645
Size: 283 362
DraIII CAC_nnn'GTG
Cuts at: 0 512 645
Size: 512 133
EaeI y'GGCC_r
Cuts at: 0 237 645
Size: 237 408
EarI CTCTTCn'nnn_
Cuts at: 0 142 557 645
Size: 142 415 88
Fragments arranged by size:
415 142 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 645
Size: 467 178
EcoO109I rG'GnC_Cy
Cuts at: 0 294 645
Size: 294 351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 232 645
Size: 232 413
HaeII r_GCGC'y
Cuts at: 0 273 645
Size: 273 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 645
Size: 445 32 168
Fragments arranged by size:
445 168 32
KasI G'GCGC_C
Cuts at: 0 269 645
Size: 269 376
KpnI G_GTAC'C
Cuts at: 0 634 645
Size: 634 11
MluI A'CGCG_T
Cuts at: 0 581 645
Size: 581 64
MscI TGG'CCA
Cuts at: 0 239 645
Size: 239 406
MslI CAynn'nnrTG
Cuts at: 0 172 175 316 463 526 645
Size: 172 3 141 147 63 119
Fragments arranged by size:
172 147 141 119 63 3
NarI GG'CG_CC
Cuts at: 0 270 645
Size: 270 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 249 645
Size: 249 396
PpuMI rG'GwC_Cy
Cuts at: 0 294 645
Size: 294 351
PstI C_TGCA'G
Cuts at: 0 278 302 645
Size: 278 24 343
Fragments arranged by size:
343 278 24
SbfI CC_TGCA'GG
Cuts at: 0 302 645
Size: 302 343
SfcI C'TryA_G
Cuts at: 0 189 274 298 540 645
Size: 189 85 24 242 105
Fragments arranged by size:
242 189 105 85 24
SfoI GGC'GCC
Cuts at: 0 271 645
Size: 271 374
SmaI CCC'GGG
Cuts at: 0 370 645
Size: 370 275
TatI w'GTAC_w
Cuts at: 0 485 645
Size: 485 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 645
Size: 174 471
XmaI C'CCGG_G
Cuts at: 0 368 645
Size: 368 277
Enzymes that do cut:
AarI Acc65I AflIII AleI AlfI AlwNI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI
BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI
BtgI BtsI DraIII EaeI EarI Eco57MI EcoO109I FalI
HaeII Hin4I KasI KpnI MluI MscI MslI NarI
PflMI PpuMI PstI SbfI SfcI SfoI SmaI TatI
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaWI BseYI
BsiEI BsiWI BsmI BspEI BsrBI BsrFI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MmeI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI ZraI