Restriction map for
pF1KA1159
(Linear) (Six-base) MAPSORT of: KIAA1159.seq Check: 6012 from: 1 to: 756
KIAA1159.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AccI GT'mk_AC
Cuts at: 0 662 756
Size: 662 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 756
Size: 372 384
AvaI C'yCGr_G
Cuts at: 0 131 599 756
Size: 131 468 157
Fragments arranged by size:
468 157 131
AvrII C'CTAG_G
Cuts at: 0 195 756
Size: 195 561
BanI G'GyrC_C
Cuts at: 0 135 756
Size: 135 621
BanII G_rGCy'C
Cuts at: 0 619 756
Size: 619 137
BbvCI CC'TCA_GC
Cuts at: 0 276 756
Size: 276 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 756
Size: 26 730
BglII A'GATC_T
Cuts at: 0 485 590 756
Size: 485 105 166
Fragments arranged by size:
485 166 105
BlpI GC'TnA_GC
Cuts at: 0 618 756
Size: 618 138
Bme1580I G_kGCm'C
Cuts at: 0 449 712 756
Size: 449 263 44
Fragments arranged by size:
449 263 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 756
Size: 446 310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 756
Size: 648 108
Bpu10I CC'TnA_GC
Cuts at: 0 98 276 756
Size: 98 178 480
Fragments arranged by size:
480 178 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 596 756
Size: 596 160
BsaBI GATnn'nnATC
Cuts at: 0 490 756
Size: 490 266
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 756
Size: 301 30 425
Fragments arranged by size:
425 301 30
BseYI C'CCAG_C
Cuts at: 0 302 582 638 756
Size: 302 280 56 118
Fragments arranged by size:
302 280 118 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 756
Size: 55 701
BsiHKAI G_wGCw'C
Cuts at: 0 619 756
Size: 619 137
BsmI GAATG_Cn'
Cuts at: 0 230 756
Size: 230 526
Bsp1286I G_dGCh'C
Cuts at: 0 449 619 712 756
Size: 449 170 93 44
Fragments arranged by size:
449 170 93 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 756
Size: 353 403
BsrDI GCAATG_nn'
Cuts at: 0 384 756
Size: 384 372
BssSI C'ACGA_G
Cuts at: 0 112 442 756
Size: 112 330 314
Fragments arranged by size:
330 314 112
BstXI CCAn_nnnn'nTGG
Cuts at: 0 73 379 756
Size: 73 306 377
Fragments arranged by size:
377 306 73
BstYI r'GATC_y
Cuts at: 0 485 590 756
Size: 485 105 166
Fragments arranged by size:
485 166 105
BtgI C'CryG_G
Cuts at: 0 176 372 756
Size: 176 196 384
Fragments arranged by size:
384 196 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 651 756
Size: 651 105
EaeI y'GGCC_r
Cuts at: 0 63 179 756
Size: 63 116 577
Fragments arranged by size:
577 116 63
EcoICRI GAG'CTC
Cuts at: 0 617 756
Size: 617 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 648 756
Size: 372 276 108
Fragments arranged by size:
372 276 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 756
Size: 34 722
EcoO109I rG'GnC_Cy
Cuts at: 0 93 383 756
Size: 93 290 373
Fragments arranged by size:
373 290 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 756
Size: 312 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 756
Size: 301 32 423
Fragments arranged by size:
423 301 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 426 756
Size: 344 82 330
Fragments arranged by size:
344 330 82
MscI TGG'CCA
Cuts at: 0 65 181 756
Size: 65 116 575
Fragments arranged by size:
575 116 65
MslI CAynn'nnrTG
Cuts at: 0 377 756
Size: 377 379
NcoI C'CATG_G
Cuts at: 0 176 372 756
Size: 176 196 384
Fragments arranged by size:
384 196 176
PpuMI rG'GwC_Cy
Cuts at: 0 93 383 756
Size: 93 290 373
Fragments arranged by size:
373 290 93
SacI G_AGCT'C
Cuts at: 0 619 756
Size: 619 137
SfcI C'TryA_G
Cuts at: 0 461 675 756
Size: 461 214 81
Fragments arranged by size:
461 214 81
SmaI CCC'GGG
Cuts at: 0 133 756
Size: 133 623
StuI AGG'CCT
Cuts at: 0 505 756
Size: 505 251
StyI C'CwwG_G
Cuts at: 0 176 195 281 372 500 756
Size: 176 19 86 91 128 256
Fragments arranged by size:
256 176 128 91 86 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 756
Size: 589 167
XmaI C'CCGG_G
Cuts at: 0 131 756
Size: 131 625
Enzymes that do cut:
AccI AcuI AvaI AvrII BanI BanII BbvCI BcgI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BssSI BstXI BstYI BtgI BtgZI EaeI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I MmeI MscI MslI NcoI
PpuMI SacI SfcI SmaI StuI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglI BmgBI BmtI BplI BpuEI BsaAI
BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI