Restriction map for
pF1KA1160
(Linear) (Six-base) MAPSORT of: KIAA1160.seq Check: 1794 from: 1 to: 993
KIAA1160.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AflIII A'CryG_T
Cuts at: 0 439 993
Size: 439 554
ApoI r'AATT_y
Cuts at: 0 199 206 993
Size: 199 7 787
Fragments arranged by size:
787 199 7
BanI G'GyrC_C
Cuts at: 0 971 993
Size: 971 22
BanII G_rGCy'C
Cuts at: 0 452 960 993
Size: 452 508 33
Fragments arranged by size:
508 452 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 993
Size: 90 903
BglI GCCn_nnn'nGGC
Cuts at: 0 28 924 993
Size: 28 896 69
Fragments arranged by size:
896 69 28
BglII A'GATC_T
Cuts at: 0 155 856 993
Size: 155 701 137
Fragments arranged by size:
701 155 137
BlpI GC'TnA_GC
Cuts at: 0 53 446 993
Size: 53 393 547
Fragments arranged by size:
547 393 53
BmgBI CAC'GTC
Cuts at: 0 726 993
Size: 726 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 267 993
Size: 267 726
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 599 993
Size: 599 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 993
Size: 775 218
Bpu10I CC'TnA_GC
Cuts at: 0 33 241 993
Size: 33 208 752
Fragments arranged by size:
752 208 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 993
Size: 802 191
BsaAI yAC'GTr
Cuts at: 0 442 993
Size: 442 551
BsaBI GATnn'nnATC
Cuts at: 0 637 993
Size: 637 356
BsaWI w'CCGG_w
Cuts at: 0 266 993
Size: 266 727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 611 625 628 637 670 673 769
Size: 416 195 14 3 9 33 3 96
Cuts at: 769 778 968 993
Size: 9 190 25
Fragments arranged by size:
416 195 190 96 33 25 14 9
9 3 3
BseYI C'CCAG_C
Cuts at: 0 279 993
Size: 279 714
BsiEI CG_ry'CG
Cuts at: 0 871 993
Size: 871 122
BsiHKAI G_wGCw'C
Cuts at: 0 452 960 993
Size: 452 508 33
Fragments arranged by size:
508 452 33
BsmI GAATG_Cn'
Cuts at: 0 189 993
Size: 189 804
BsmBI CGTCTCn'nnnn_
Cuts at: 0 129 993
Size: 129 864
Bsp1286I G_dGCh'C
Cuts at: 0 452 960 993
Size: 452 508 33
Fragments arranged by size:
508 452 33
BspEI T'CCGG_A
Cuts at: 0 266 993
Size: 266 727
BspHI T'CATG_A
Cuts at: 0 324 452 993
Size: 324 128 541
Fragments arranged by size:
541 324 128
BsrBI CCG'CTC
Cuts at: 0 881 993
Size: 881 112
BsrDI GCAATG_nn'
Cuts at: 0 716 993
Size: 716 277
BsrFI r'CCGG_y
Cuts at: 0 870 993
Size: 870 123
BstBI TT'CG_AA
Cuts at: 0 204 993
Size: 204 789
BstEII G'GTnAC_C
Cuts at: 0 481 993
Size: 481 512
BstYI r'GATC_y
Cuts at: 0 155 856 993
Size: 155 701 137
Fragments arranged by size:
701 155 137
BtsI GCAGTG_nn'
Cuts at: 0 920 993
Size: 920 73
ClaI AT'CG_AT
Cuts at: 0 464 993
Size: 464 529
EaeI y'GGCC_r
Cuts at: 0 868 872 993
Size: 868 4 121
Fragments arranged by size:
868 121 4
EagI C'GGCC_G
Cuts at: 0 868 993
Size: 868 125
EarI CTCTTCn'nnn_
Cuts at: 0 56 425 602 614 623 993
Size: 56 369 177 12 9 370
Fragments arranged by size:
370 369 177 56 12 9
EcoICRI GAG'CTC
Cuts at: 0 450 958 993
Size: 450 508 35
Fragments arranged by size:
508 450 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 993
Size: 775 218
EcoO109I rG'GnC_Cy
Cuts at: 0 286 993
Size: 286 707
EcoRI G'AATT_C
Cuts at: 0 206 993
Size: 206 787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 993
Size: 436 557
FseI GG_CCGG'CC
Cuts at: 0 874 993
Size: 874 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 646 678 993
Size: 128 32 486 32 315
Fragments arranged by size:
486 315 128 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 993
Size: 644 349
MspA1I CmG'CkG
Cuts at: 0 57 993
Size: 57 936
NaeI GCC'GGC
Cuts at: 0 872 993
Size: 872 121
NgoMIV G'CCGG_C
Cuts at: 0 870 993
Size: 870 123
PmlI CAC'GTG
Cuts at: 0 442 993
Size: 442 551
PpuMI rG'GwC_Cy
Cuts at: 0 286 993
Size: 286 707
PstI C_TGCA'G
Cuts at: 0 818 856 993
Size: 818 38 137
Fragments arranged by size:
818 137 38
PvuII CAG'CTG
Cuts at: 0 57 993
Size: 57 936
SacI G_AGCT'C
Cuts at: 0 452 960 993
Size: 452 508 33
Fragments arranged by size:
508 452 33
SbfI CC_TGCA'GG
Cuts at: 0 818 993
Size: 818 175
ScaI AGT'ACT
Cuts at: 0 364 475 993
Size: 364 111 518
Fragments arranged by size:
518 364 111
SfcI C'TryA_G
Cuts at: 0 814 852 993
Size: 814 38 141
Fragments arranged by size:
814 141 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 28 993
Size: 28 965
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 912 993
Size: 912 81
TatI w'GTAC_w
Cuts at: 0 106 362 473 993
Size: 106 256 111 520
Fragments arranged by size:
520 256 111 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 843 993
Size: 843 150
XmnI GAAnn'nnTTC
Cuts at: 0 419 993
Size: 419 574
Enzymes that do cut:
AflIII ApoI BanI BanII BbsI BglI BglII BlpI
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BstBI BstEII BstYI
BtsI ClaI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI Hin4I MmeI MspA1I NaeI NgoMIV
PmlI PpuMI PstI PvuII SacI SbfI ScaI SfcI
SfiI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI Bme1580I BmtI BpuEI
BsaHI BsaXI BsgI BsiWI BspMI BsrGI BssHII BssSI
BstAPI BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraI
DraIII DrdI EciI EcoNI EcoRV FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SexAI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI