Restriction map for
pF1KA1168
(Linear) (Six-base) MAPSORT of: KIAA1168.seq Check: 9458 from: 1 to: 3834
KIAA1168.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 897 1371 3626 3834
Size: 897 474 2255 208
Fragments arranged by size:
2255 897 474 208
AccI GT'mk_AC
Cuts at: 0 2180 3176 3834
Size: 2180 996 658
Fragments arranged by size:
2180 996 658
AclI AA'CG_TT
Cuts at: 0 2247 3834
Size: 2247 1587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2341 3031 3694 3834
Size: 2341 690 663 140
Fragments arranged by size:
2341 690 663 140
AflIII A'CryG_T
Cuts at: 0 497 2331 3047 3834
Size: 497 1834 716 787
Fragments arranged by size:
1834 787 716 497
AhdI GACnn_n'nnGTC
Cuts at: 0 1834 3834
Size: 1834 2000
AlwNI CAG_nnn'CTG
Cuts at: 0 582 2130 3060 3834
Size: 582 1548 930 774
Fragments arranged by size:
1548 930 774 582
ApaI G_GGCC'C
Cuts at: 0 3406 3834
Size: 3406 428
ApaLI G'TGCA_C
Cuts at: 0 999 3834
Size: 999 2835
ApoI r'AATT_y
Cuts at: 0 889 1339 1945 2790 3834
Size: 889 450 606 845 1044
Fragments arranged by size:
1044 889 845 606 450
AseI AT'TA_AT
Cuts at: 0 869 3834
Size: 869 2965
AvaI C'yCGr_G
Cuts at: 0 269 1686 3834
Size: 269 1417 2148
Fragments arranged by size:
2148 1417 269
BamHI G'GATC_C
Cuts at: 0 2740 2984 3834
Size: 2740 244 850
Fragments arranged by size:
2740 850 244
BanI G'GyrC_C
Cuts at: 0 912 1285 1635 3834
Size: 912 373 350 2199
Fragments arranged by size:
2199 912 373 350
BanII G_rGCy'C
Cuts at: 0 1088 1123 1166 1481 1577 3038 3406 3834
Size: 1088 35 43 315 96 1461 368 428
Fragments arranged by size:
1461 1088 428 368 315 96 43 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 334 1401 1810 2592 3834
Size: 334 1067 409 782 1242
Fragments arranged by size:
1242 1067 782 409 334
BbvCI CC'TCA_GC
Cuts at: 0 2150 3278 3834
Size: 2150 1128 556
Fragments arranged by size:
2150 1128 556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 378 412 1715 1749 3834
Size: 378 34 1303 34 2085
Fragments arranged by size:
2085 1303 378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2300 3834
Size: 2300 1534
BclI T'GATC_A
Cuts at: 0 1367 2169 3834
Size: 1367 802 1665
Fragments arranged by size:
1665 1367 802
BfrBI ATG'CAT
Cuts at: 0 83 151 3834
Size: 83 68 3683
Fragments arranged by size:
3683 83 68
BglI GCCn_nnn'nGGC
Cuts at: 0 1485 1730 3834
Size: 1485 245 2104
Fragments arranged by size:
2104 1485 245
BglII A'GATC_T
Cuts at: 0 317 2198 3707 3834
Size: 317 1881 1509 127
Fragments arranged by size:
1881 1509 317 127
BlpI GC'TnA_GC
Cuts at: 0 3518 3834
Size: 3518 316
Bme1580I G_kGCm'C
Cuts at: 0 917 1003 1640 3406 3671 3834
Size: 917 86 637 1766 265 163
Fragments arranged by size:
1766 917 637 265 163 86
BmgBI CAC'GTC
Cuts at: 0 12 998 1218 2334 3048 3432 3834
Size: 12 986 220 1116 714 384 402
Fragments arranged by size:
1116 986 714 402 384 220 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 597 657 764 1017 1566 3555 3834
Size: 597 60 107 253 549 1989 279
Fragments arranged by size:
1989 597 549 279 253 107 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1248 2991 3834
Size: 1248 1743 843
Fragments arranged by size:
1743 1248 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 238 364 1975 2485 2497 2893 3010
Size: 183 55 126 1611 510 12 396 117
Cuts at: 3010 3220 3834
Size: 210 614
Fragments arranged by size:
1611 614 510 396 210 183 126 117
55 12
Bpu10I CC'TnA_GC
Cuts at: 0 1122 2150 3278 3834
Size: 1122 1028 1128 556
Fragments arranged by size:
1128 1122 1028 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1614 3834
Size: 775 839 2220
Fragments arranged by size:
2220 839 775
BsaI GGTCTCn'nnnn_
Cuts at: 0 952 3834
Size: 952 2882
BsaHI Gr'CG_yC
Cuts at: 0 2276 3422 3834
Size: 2276 1146 412
Fragments arranged by size:
2276 1146 412
BsaWI w'CCGG_w
Cuts at: 0 3206 3689 3834
Size: 3206 483 145
Fragments arranged by size:
3206 483 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 770 800 2434 2452 2464 2482 3313 3343
Size: 770 30 1634 18 12 18 831 30
Cuts at: 3343 3834
Size: 491
Fragments arranged by size:
1634 831 770 491 30 30 18 18
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 718 778 1165 1827 3085 3136 3834
Size: 718 60 387 662 1258 51 698
Fragments arranged by size:
1258 718 698 662 387 60 51
BseYI C'CCAG_C
Cuts at: 0 1928 2378 3267 3573 3834
Size: 1928 450 889 306 261
Fragments arranged by size:
1928 889 450 306 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 3649 3834
Size: 555 3094 185
Fragments arranged by size:
3094 555 185
BsiEI CG_ry'CG
Cuts at: 0 3690 3834
Size: 3690 144
BsiHKAI G_wGCw'C
Cuts at: 0 969 1003 1088 1166 1388 3038 3834
Size: 969 34 85 78 222 1650 796
Fragments arranged by size:
1650 969 796 222 85 78 34
BsmI GAATG_Cn'
Cuts at: 0 153 3368 3834
Size: 153 3215 466
Fragments arranged by size:
3215 466 153
Bsp1286I G_dGCh'C
Cuts at: 0 917 969 1003 1088 1123 1166 1388 1481
Size: 917 52 34 85 35 43 222 93
Cuts at: 1481 1577 1640 3038 3406 3671 3834
Size: 96 63 1398 368 265 163
Fragments arranged by size:
1398 917 368 265 222 163 96 93
85 63 52 43 35 34
BspEI T'CCGG_A
Cuts at: 0 3206 3834
Size: 3206 628
BspHI T'CATG_A
Cuts at: 0 365 3834
Size: 365 3469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 897 1371 2718 3389 3626 3834
Size: 53 844 474 1347 671 237 208
Fragments arranged by size:
1347 844 671 474 237 208 53
BsrBI CCG'CTC
Cuts at: 0 402 3240 3264 3324 3834
Size: 402 2838 24 60 510
Fragments arranged by size:
2838 510 402 60 24
BsrDI GCAATG_nn'
Cuts at: 0 164 1444 1778 2756 3081 3290 3834
Size: 164 1280 334 978 325 209 544
Fragments arranged by size:
1280 978 544 334 325 209 164
BsrFI r'CCGG_y
Cuts at: 0 2486 3455 3834
Size: 2486 969 379
Fragments arranged by size:
2486 969 379
BsrGI T'GTAC_A
Cuts at: 0 248 1748 2081 3834
Size: 248 1500 333 1753
Fragments arranged by size:
1753 1500 333 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 912 2502 3834
Size: 912 1590 1332
Fragments arranged by size:
1590 1332 912
BstBI TT'CG_AA
Cuts at: 0 1539 3834
Size: 1539 2295
BstEII G'GTnAC_C
Cuts at: 0 354 3304 3834
Size: 354 2950 530
Fragments arranged by size:
2950 530 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 3834
Size: 639 3195
BstYI r'GATC_y
Cuts at: 0 317 597 1052 2076 2198 2740 2984 3707
Size: 317 280 455 1024 122 542 244 723
Cuts at: 3707 3834
Size: 127
Fragments arranged by size:
1024 723 542 455 317 280 244 127
122
Bsu36I CC'TnA_GG
Cuts at: 0 1810 3061 3834
Size: 1810 1251 773
Fragments arranged by size:
1810 1251 773
BtgI C'CryG_G
Cuts at: 0 1289 1964 2219 3755 3834
Size: 1289 675 255 1536 79
Fragments arranged by size:
1536 1289 675 255 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2832 3548 3834
Size: 2832 716 286
Fragments arranged by size:
2832 716 286
BtsI GCAGTG_nn'
Cuts at: 0 1918 2235 2314 3529 3834
Size: 1918 317 79 1215 305
Fragments arranged by size:
1918 1215 317 305 79
DraI TTT'AAA
Cuts at: 0 2118 3834
Size: 2118 1716
DraIII CAC_nnn'GTG
Cuts at: 0 665 2565 3834
Size: 665 1900 1269
Fragments arranged by size:
1900 1269 665
EaeI y'GGCC_r
Cuts at: 0 641 944 2580 3683 3821 3834
Size: 641 303 1636 1103 138 13
Fragments arranged by size:
1636 1103 641 303 138 13
EarI CTCTTCn'nnn_
Cuts at: 0 47 856 971 1171 1722 2878 3091 3834
Size: 47 809 115 200 551 1156 213 743
Fragments arranged by size:
1156 809 743 551 213 200 115 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2559 3834
Size: 2559 1275
EcoICRI GAG'CTC
Cuts at: 0 1086 1164 3036 3834
Size: 1086 78 1872 798
Fragments arranged by size:
1872 1086 798 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 238 364 1975 2341 2485 2497 2893
Size: 183 55 126 1611 366 144 12 396
Cuts at: 2893 3010 3031 3220 3694 3834
Size: 117 21 189 474 140
Fragments arranged by size:
1611 474 396 366 189 183 144 140
126 117 55 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 564 1486 3316 3834
Size: 564 922 1830 518
Fragments arranged by size:
1830 922 564 518
EcoO109I rG'GnC_Cy
Cuts at: 0 1608 2442 3272 3387 3402 3834
Size: 1608 834 830 115 15 432
Fragments arranged by size:
1608 834 830 432 115 15
EcoRI G'AATT_C
Cuts at: 0 1945 3834
Size: 1945 1889
EcoRV GAT'ATC
Cuts at: 0 1629 3834
Size: 1629 2205
HaeII r_GCGC'y
Cuts at: 0 1214 2096 3198 3470 3834
Size: 1214 882 1102 272 364
Fragments arranged by size:
1214 1102 882 364 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 302 334 442 474 582 589
Size: 16 32 254 32 108 32 108 7
Cuts at: 589 614 621 855 887 951 970 983 1002
Size: 25 7 234 32 64 19 13 19
Cuts at: 1002 1613 1645 1820 1852 2434 2466 3164 3196
Size: 611 32 175 32 582 32 698 32
Cuts at: 3196 3201 3233 3834
Size: 5 32 601
Fragments arranged by size:
698 611 601 582 254 234 175 108
108 64 32 32 32 32 32 32
32 32 32 25 19 19 16 13
7 7 5
HincII GTy'rAC
Cuts at: 0 726 1961 3834
Size: 726 1235 1873
Fragments arranged by size:
1873 1235 726
HpaI GTT'AAC
Cuts at: 0 1961 3834
Size: 1961 1873
MfeI C'AATT_G
Cuts at: 0 2354 3834
Size: 2354 1480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 2219 3834
Size: 59 2160 1615
Fragments arranged by size:
2160 1615 59
MscI TGG'CCA
Cuts at: 0 643 946 3823 3834
Size: 643 303 2877 11
Fragments arranged by size:
2877 643 303 11
MslI CAynn'nnrTG
Cuts at: 0 233 496 733 1225 1993 2851 2969 3434
Size: 233 263 237 492 768 858 118 465
Cuts at: 3434 3493 3834
Size: 59 341
Fragments arranged by size:
858 768 492 465 341 263 237 233
118 59
MspA1I CmG'CkG
Cuts at: 0 263 903 1291 2340 3009 3517 3834
Size: 263 640 388 1049 669 508 317
Fragments arranged by size:
1049 669 640 508 388 317 263
NcoI C'CATG_G
Cuts at: 0 1964 2219 3834
Size: 1964 255 1615
Fragments arranged by size:
1964 1615 255
NdeI CA'TA_TG
Cuts at: 0 782 2516 3834
Size: 782 1734 1318
Fragments arranged by size:
1734 1318 782
NruI TCG'CGA
Cuts at: 0 1160 3299 3834
Size: 1160 2139 535
Fragments arranged by size:
2139 1160 535
NsiI A_TGCA'T
Cuts at: 0 85 153 3834
Size: 85 68 3681
Fragments arranged by size:
3681 85 68
NspI r_CATG'y
Cuts at: 0 501 933 2599 3787 3834
Size: 501 432 1666 1188 47
Fragments arranged by size:
1666 1188 501 432 47
PasI CC'CwG_GG
Cuts at: 0 2980 3834
Size: 2980 854
PciI A'CATG_T
Cuts at: 0 497 3834
Size: 497 3337
PflMI CCAn_nnn'nTGG
Cuts at: 0 1221 2006 2028 2343 3819 3834
Size: 1221 785 22 315 1476 15
Fragments arranged by size:
1476 1221 785 315 22 15
PfoI T'CCnGG_A
Cuts at: 0 509 611 1055 1952 2027 2987 3834
Size: 509 102 444 897 75 960 847
Fragments arranged by size:
960 897 847 509 444 102 75
PpuMI rG'GwC_Cy
Cuts at: 0 1608 2442 3272 3387 3834
Size: 1608 834 830 115 447
Fragments arranged by size:
1608 834 830 447 115
PspOMI G'GGCC_C
Cuts at: 0 3402 3834
Size: 3402 432
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 742 774 1420 1452 3834
Size: 742 32 646 32 2382
Fragments arranged by size:
2382 742 646 32 32
PstI C_TGCA'G
Cuts at: 0 412 908 1193 1382 1910 2827 3386 3834
Size: 412 496 285 189 528 917 559 448
Fragments arranged by size:
917 559 528 496 448 412 285 189
PvuII CAG'CTG
Cuts at: 0 263 903 2340 3009 3517 3834
Size: 263 640 1437 669 508 317
Fragments arranged by size:
1437 669 640 508 317 263
SacI G_AGCT'C
Cuts at: 0 1088 1166 3038 3834
Size: 1088 78 1872 796
Fragments arranged by size:
1872 1088 796 78
SacII CC_GC'GG
Cuts at: 0 1292 3834
Size: 1292 2542
SanDI GG'GwC_CC
Cuts at: 0 3272 3834
Size: 3272 562
SapI GCTCTTCn'nnn_
Cuts at: 0 47 1171 2878 3834
Size: 47 1124 1707 956
Fragments arranged by size:
1707 1124 956 47
SbfI CC_TGCA'GG
Cuts at: 0 1382 3386 3834
Size: 1382 2004 448
Fragments arranged by size:
2004 1382 448
SexAI A'CCwGG_T
Cuts at: 0 3245 3834
Size: 3245 589
SfcI C'TryA_G
Cuts at: 0 188 408 904 1095 1189 1378 1528 1906
Size: 188 220 496 191 94 189 150 378
Cuts at: 1906 2763 2775 2784 2823 3382 3834
Size: 857 12 9 39 559 452
Fragments arranged by size:
857 559 496 452 378 220 191 189
188 150 94 39 12 9
SmaI CCC'GGG
Cuts at: 0 271 3834
Size: 271 3563
SmlI C'TyrA_G
Cuts at: 0 790 1593 3834
Size: 790 803 2241
Fragments arranged by size:
2241 803 790
StuI AGG'CCT
Cuts at: 0 466 1376 1672 3834
Size: 466 910 296 2162
Fragments arranged by size:
2162 910 466 296
StyI C'CwwG_G
Cuts at: 0 480 1964 2219 2408 2895 3317 3818 3834
Size: 480 1484 255 189 487 422 501 16
Fragments arranged by size:
1484 501 487 480 422 255 189 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 1018 2701 3819 3834
Size: 676 342 1683 1118 15
Fragments arranged by size:
1683 1118 676 342 15
TatI w'GTAC_w
Cuts at: 0 248 1025 1229 1748 2081 3482 3834
Size: 248 777 204 519 333 1401 352
Fragments arranged by size:
1401 777 519 352 333 248 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1849 1881 2452 2484 2551 2583 3313 3345
Size: 1849 32 571 32 67 32 730 32
Cuts at: 3345 3834
Size: 489
Fragments arranged by size:
1849 730 571 489 67 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 454 721 3834
Size: 454 267 3113
Fragments arranged by size:
3113 454 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2460 3834
Size: 2460 1374
XmaI C'CCGG_G
Cuts at: 0 269 3834
Size: 269 3565
XmnI GAAnn'nnTTC
Cuts at: 0 56 3834
Size: 56 3778
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NruI NsiI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AleI AlfI AloI
AscI AsiSI AvrII BaeI BbeI BmtI BsaAI BsaBI
BsiWI BsmBI BssHII BssSI BstZ17I ClaI CspCI DrdI
EagI FalI FseI FspI FspAI HindIII KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SalI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI