Restriction map for
pF1KSDA1169
(Linear) (Six-base) MAPSORT of: KIAA1169.seq Check: 5416 from: 1 to: 2448
KIAA1169.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 771 1495 2329 2448
Size: 758 13 724 834 119
Fragments arranged by size:
834 758 724 119 13
AfeI AGC'GCT
Cuts at: 0 1135 1654 2431 2448
Size: 1135 519 777 17
Fragments arranged by size:
1135 777 519 17
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 2448
Size: 630 1818
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1396 1428 1663 1695 2448
Size: 868 32 496 32 235 32 753
Fragments arranged by size:
868 753 496 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 684 1629 2448
Size: 684 945 819
Fragments arranged by size:
945 819 684
ApaI G_GGCC'C
Cuts at: 0 1495 2448
Size: 1495 953
AseI AT'TA_AT
Cuts at: 0 1301 2448
Size: 1301 1147
AvaI C'yCGr_G
Cuts at: 0 602 2059 2448
Size: 602 1457 389
Fragments arranged by size:
1457 602 389
BamHI G'GATC_C
Cuts at: 0 1370 2448
Size: 1370 1078
BanII G_rGCy'C
Cuts at: 0 1495 1592 1928 2177 2448
Size: 1495 97 336 249 271
Fragments arranged by size:
1495 336 271 249 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 885 1502 1770 2064 2448
Size: 885 617 268 294 384
Fragments arranged by size:
885 617 384 294 268
BbvCI CC'TCA_GC
Cuts at: 0 1630 2448
Size: 1630 818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1198 1232 2448
Size: 1198 34 1216
Fragments arranged by size:
1216 1198 34
BclI T'GATC_A
Cuts at: 0 728 2448
Size: 728 1720
BglI GCCn_nnn'nGGC
Cuts at: 0 67 2448
Size: 67 2381
BglII A'GATC_T
Cuts at: 0 671 2448
Size: 671 1777
BlpI GC'TnA_GC
Cuts at: 0 1180 2448
Size: 1180 1268
Bme1580I G_kGCm'C
Cuts at: 0 63 1427 1495 2448
Size: 63 1364 68 953
Fragments arranged by size:
1364 953 68 63
BmgBI CAC'GTC
Cuts at: 0 24 2448
Size: 24 2424
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 207 2448
Size: 143 64 2241
Fragments arranged by size:
2241 143 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2247 2448
Size: 2247 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 217 448 817 1449 1606 2007 2191
Size: 165 52 231 369 632 157 401 184
Cuts at: 2191 2242 2448
Size: 51 206
Fragments arranged by size:
632 401 369 231 206 184 165 157
52 51
Bpu10I CC'TnA_GC
Cuts at: 0 1630 2302 2448
Size: 1630 672 146
Fragments arranged by size:
1630 672 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2152 2448
Size: 2152 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 2218 2448
Size: 2218 230
BsaAI yAC'GTr
Cuts at: 0 1422 2448
Size: 1422 1026
BsaBI GATnn'nnATC
Cuts at: 0 2038 2448
Size: 2038 410
BsaHI Gr'CG_yC
Cuts at: 0 650 1964 2448
Size: 650 1314 484
Fragments arranged by size:
1314 650 484
BsaWI w'CCGG_w
Cuts at: 0 1513 2448
Size: 1513 935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 745 775 1396 1426 1492 1522 2448
Size: 745 30 621 30 66 30 926
Fragments arranged by size:
926 745 621 66 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1071 1273 2190 2338 2359 2448
Size: 1071 202 917 148 21 89
Fragments arranged by size:
1071 917 202 148 89 21
BseYI C'CCAG_C
Cuts at: 0 483 2405 2448
Size: 483 1922 43
Fragments arranged by size:
1922 483 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 991 2411 2448
Size: 565 426 1420 37
Fragments arranged by size:
1420 565 426 37
BsiHKAI G_wGCw'C
Cuts at: 0 609 1253 1928 2177 2448
Size: 609 644 675 249 271
Fragments arranged by size:
675 644 609 271 249
Bsp1286I G_dGCh'C
Cuts at: 0 63 609 1253 1427 1495 1592 1928 2177
Size: 63 546 644 174 68 97 336 249
Cuts at: 2177 2448
Size: 271
Fragments arranged by size:
644 546 336 271 249 174 97 68
63
BspEI T'CCGG_A
Cuts at: 0 1513 2448
Size: 1513 935
BspHI T'CATG_A
Cuts at: 0 725 932 2003 2448
Size: 725 207 1071 445
Fragments arranged by size:
1071 725 445 207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 947 2448
Size: 671 276 1501
Fragments arranged by size:
1501 671 276
BsrBI CCG'CTC
Cuts at: 0 30 2448
Size: 30 2418
BsrFI r'CCGG_y
Cuts at: 0 1066 1483 2448
Size: 1066 417 965
Fragments arranged by size:
1066 965 417
BssSI C'ACGA_G
Cuts at: 0 1375 1797 2448
Size: 1375 422 651
Fragments arranged by size:
1375 651 422
BstEII G'GTnAC_C
Cuts at: 0 597 2448
Size: 597 1851
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 288 2220 2448
Size: 84 204 1932 228
Fragments arranged by size:
1932 228 204 84
BstYI r'GATC_y
Cuts at: 0 671 1370 2327 2448
Size: 671 699 957 121
Fragments arranged by size:
957 699 671 121
BtgI C'CryG_G
Cuts at: 0 341 518 1838 1853 2024 2261 2448
Size: 341 177 1320 15 171 237 187
Fragments arranged by size:
1320 341 237 187 177 171 15
BtsI GCAGTG_nn'
Cuts at: 0 63 607 2394 2448
Size: 63 544 1787 54
Fragments arranged by size:
1787 544 63 54
DraIII CAC_nnn'GTG
Cuts at: 0 1839 2448
Size: 1839 609
EaeI y'GGCC_r
Cuts at: 0 338 1700 2448
Size: 338 1362 748
Fragments arranged by size:
1362 748 338
EarI CTCTTCn'nnn_
Cuts at: 0 89 325 2147 2448
Size: 89 236 1822 301
Fragments arranged by size:
1822 301 236 89
EcoICRI GAG'CTC
Cuts at: 0 1926 2175 2448
Size: 1926 249 273
Fragments arranged by size:
1926 273 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 217 448 758 771 817 1449 1495
Size: 165 52 231 310 13 46 632 46
Cuts at: 1495 1606 2007 2191 2242 2329 2448
Size: 111 401 184 51 87 119
Fragments arranged by size:
632 401 310 231 184 165 119 111
87 52 51 46 46 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 2134 2448
Size: 373 1761 314
Fragments arranged by size:
1761 373 314
EcoO109I rG'GnC_Cy
Cuts at: 0 144 378 1152 1492 2195 2448
Size: 144 234 774 340 703 253
Fragments arranged by size:
774 703 340 253 234 144
EcoRV GAT'ATC
Cuts at: 0 1203 2448
Size: 1203 1245
HaeII r_GCGC'y
Cuts at: 0 289 1137 1282 1573 1656 2433 2448
Size: 289 848 145 291 83 777 15
Fragments arranged by size:
848 777 291 289 145 83 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 2448
Size: 1492 32 924
Fragments arranged by size:
1492 924 32
HincII GTy'rAC
Cuts at: 0 1359 1938 2448
Size: 1359 579 510
Fragments arranged by size:
1359 579 510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 2448
Size: 101 2347
MscI TGG'CCA
Cuts at: 0 340 1702 2448
Size: 340 1362 746
Fragments arranged by size:
1362 746 340
MslI CAynn'nnrTG
Cuts at: 0 565 1954 2029 2448
Size: 565 1389 75 419
Fragments arranged by size:
1389 565 419 75
MspA1I CmG'CkG
Cuts at: 0 1626 1823 2448
Size: 1626 197 625
Fragments arranged by size:
1626 625 197
NaeI GCC'GGC
Cuts at: 0 1068 1485 2448
Size: 1068 417 963
Fragments arranged by size:
1068 963 417
NcoI C'CATG_G
Cuts at: 0 518 2024 2261 2448
Size: 518 1506 237 187
Fragments arranged by size:
1506 518 237 187
NgoMIV G'CCGG_C
Cuts at: 0 1066 1483 2448
Size: 1066 417 965
Fragments arranged by size:
1066 965 417
NspI r_CATG'y
Cuts at: 0 1033 2353 2448
Size: 1033 1320 95
Fragments arranged by size:
1320 1033 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 1839 2448
Size: 1839 609
PfoI T'CCnGG_A
Cuts at: 0 233 871 2168 2330 2448
Size: 233 638 1297 162 118
Fragments arranged by size:
1297 638 233 162 118
PmlI CAC'GTG
Cuts at: 0 1422 2448
Size: 1422 1026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1396 1428 2089 2121 2448
Size: 1396 32 661 32 327
Fragments arranged by size:
1396 661 327 32 32
PshAI GACnn'nnGTC
Cuts at: 0 520 2448
Size: 520 1928
PspOMI G'GGCC_C
Cuts at: 0 1491 2448
Size: 1491 957
PspXI vC'TCGA_Gb
Cuts at: 0 2059 2448
Size: 2059 389
PvuII CAG'CTG
Cuts at: 0 1626 2448
Size: 1626 822
SacI G_AGCT'C
Cuts at: 0 1928 2177 2448
Size: 1928 249 271
Fragments arranged by size:
1928 271 249
SapI GCTCTTCn'nnn_
Cuts at: 0 89 2147 2448
Size: 89 2058 301
Fragments arranged by size:
2058 301 89
ScaI AGT'ACT
Cuts at: 0 1504 2448
Size: 1504 944
SfcI C'TryA_G
Cuts at: 0 1077 2085 2448
Size: 1077 1008 363
Fragments arranged by size:
1077 1008 363
SmlI C'TyrA_G
Cuts at: 0 2059 2131 2448
Size: 2059 72 317
Fragments arranged by size:
2059 317 72
SphI G_CATG'C
Cuts at: 0 1033 2353 2448
Size: 1033 1320 95
Fragments arranged by size:
1320 1033 95
StuI AGG'CCT
Cuts at: 0 1062 1094 1324 2065 2448
Size: 1062 32 230 741 383
Fragments arranged by size:
1062 741 383 230 32
StyI C'CwwG_G
Cuts at: 0 41 272 281 518 1319 2024 2261 2448
Size: 41 231 9 237 801 705 237 187
Fragments arranged by size:
801 705 237 237 231 187 41 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 2448
Size: 280 2168
TatI w'GTAC_w
Cuts at: 0 1340 1502 1801 2448
Size: 1340 162 299 647
Fragments arranged by size:
1340 647 299 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 1752 2154 2268 2448
Size: 567 1185 402 114 180
Fragments arranged by size:
1185 567 402 180 114
XhoI C'TCGA_G
Cuts at: 0 2059 2448
Size: 2059 389
XmnI GAAnn'nnTTC
Cuts at: 0 1408 2448
Size: 1408 1040
Enzymes that do cut:
AcuI AfeI AlfI AloI AlwNI ApaI AseI AvaI
BamHI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PmlI PpiI PshAI PspOMI PspXI
PvuII SacI SapI ScaI SfcI SmlI SphI StuI
StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AsiSI AvrII BaeI
BanI BbeI BciVI BfrBI BmtI BsiEI BsiWI BsmI
BsmBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PpuMI PsiI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XmaI ZraI