Restriction map for
pF1KSDA1171
(Linear) (Six-base) MAPSORT of: KIAA1171.seq Check: 9235 from: 1 to: 1677
KIAA1171.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 1677
Size: 288 1389
AatII G_ACGT'C
Cuts at: 0 692 1677
Size: 692 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 952 1677
Size: 124 828 725
Fragments arranged by size:
828 725 124
AfeI AGC'GCT
Cuts at: 0 640 1078 1321 1677
Size: 640 438 243 356
Fragments arranged by size:
640 438 356 243
AhdI GACnn_n'nnGTC
Cuts at: 0 1059 1677
Size: 1059 618
AleI CACnn'nnGTG
Cuts at: 0 298 1677
Size: 298 1379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 283 641 1677
Size: 109 174 358 1036
Fragments arranged by size:
1036 358 174 109
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 1677
Size: 850 32 795
Fragments arranged by size:
850 795 32
AlwNI CAG_nnn'CTG
Cuts at: 0 63 102 171 263 311 777 906 1511
Size: 63 39 69 92 48 466 129 605
Cuts at: 1511 1677
Size: 166
Fragments arranged by size:
605 466 166 129 92 69 63 48
39
ApaI G_GGCC'C
Cuts at: 0 71 135 1677
Size: 71 64 1542
Fragments arranged by size:
1542 71 64
AscI GG'CGCG_CC
Cuts at: 0 115 1677
Size: 115 1562
AvaI C'yCGr_G
Cuts at: 0 277 677 1072 1348 1677
Size: 277 400 395 276 329
Fragments arranged by size:
400 395 329 277 276
BanI G'GyrC_C
Cuts at: 0 300 1677
Size: 300 1377
BanII G_rGCy'C
Cuts at: 0 71 135 1388 1677
Size: 71 64 1253 289
Fragments arranged by size:
1253 289 71 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 838 1677
Size: 684 154 839
Fragments arranged by size:
839 684 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1063 1097 1677
Size: 1063 34 580
Fragments arranged by size:
1063 580 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 7 1677
Size: 7 1670
BclI T'GATC_A
Cuts at: 0 1337 1677
Size: 1337 340
BglI GCCn_nnn'nGGC
Cuts at: 0 403 412 1645 1677
Size: 403 9 1233 32
Fragments arranged by size:
1233 403 32 9
BlpI GC'TnA_GC
Cuts at: 0 79 1677
Size: 79 1598
Bme1580I G_kGCm'C
Cuts at: 0 71 135 305 1677
Size: 71 64 170 1372
Fragments arranged by size:
1372 170 71 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 1273 1677
Size: 138 1135 404
Fragments arranged by size:
1135 404 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 877 1254 1677
Size: 799 78 377 423
Fragments arranged by size:
799 423 377 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1677
Size: 727 950
BsaHI Gr'CG_yC
Cuts at: 0 209 689 1677
Size: 209 480 988
Fragments arranged by size:
988 480 209
BsaWI w'CCGG_w
Cuts at: 0 1279 1677
Size: 1279 398
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 1396 1426 1456 1486 1677
Size: 850 30 516 30 30 30 191
Fragments arranged by size:
850 516 191 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1101 1404 1544 1677
Size: 1101 303 140 133
Fragments arranged by size:
1101 303 140 133
BseYI C'CCAG_C
Cuts at: 0 304 1677
Size: 304 1373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 409 1336 1677
Size: 69 340 927 341
Fragments arranged by size:
927 341 340 69
BsiEI CG_ry'CG
Cuts at: 0 407 1677
Size: 407 1270
BsmI GAATG_Cn'
Cuts at: 0 324 1291 1677
Size: 324 967 386
Fragments arranged by size:
967 386 324
Bsp1286I G_dGCh'C
Cuts at: 0 71 135 305 1388 1677
Size: 71 64 170 1083 289
Fragments arranged by size:
1083 289 170 71 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 609 1475 1677
Size: 288 321 866 202
Fragments arranged by size:
866 321 288 202
BsrBI CCG'CTC
Cuts at: 0 1022 1453 1677
Size: 1022 431 224
Fragments arranged by size:
1022 431 224
BsrDI GCAATG_nn'
Cuts at: 0 489 1631 1677
Size: 489 1142 46
Fragments arranged by size:
1142 489 46
BsrFI r'CCGG_y
Cuts at: 0 402 1677
Size: 402 1275
BsrGI T'GTAC_A
Cuts at: 0 218 990 1677
Size: 218 772 687
Fragments arranged by size:
772 687 218
BssHII G'CGCG_C
Cuts at: 0 115 1677
Size: 115 1562
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 1511 1677
Size: 263 1248 166
Fragments arranged by size:
1248 263 166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1494 1677
Size: 1494 183
BstYI r'GATC_y
Cuts at: 0 347 896 1677
Size: 347 549 781
Fragments arranged by size:
781 549 347
Bsu36I CC'TnA_GG
Cuts at: 0 1310 1677
Size: 1310 367
BtgI C'CryG_G
Cuts at: 0 407 1640 1677
Size: 407 1233 37
Fragments arranged by size:
1233 407 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 810 1677
Size: 810 867
BtsI GCAGTG_nn'
Cuts at: 0 1584 1677
Size: 1584 93
ClaI AT'CG_AT
Cuts at: 0 1553 1677
Size: 1553 124
EaeI y'GGCC_r
Cuts at: 0 362 404 596 734 914 998 1136 1448
Size: 362 42 192 138 180 84 138 312
Cuts at: 1448 1536 1677
Size: 88 141
Fragments arranged by size:
362 312 192 180 141 138 138 88
84 42
EagI C'GGCC_G
Cuts at: 0 404 1677
Size: 404 1273
EarI CTCTTCn'nnn_
Cuts at: 0 955 1677
Size: 955 722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 1398 1677
Size: 865 533 279
Fragments arranged by size:
865 533 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 799 877 952 1254 1677
Size: 124 675 78 75 302 423
Fragments arranged by size:
675 423 302 124 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 700 1677
Size: 700 977
EcoO109I rG'GnC_Cy
Cuts at: 0 60 67 552 1068 1560 1662 1677
Size: 60 7 485 516 492 102 15
Fragments arranged by size:
516 492 485 102 60 15 7
FspI TGC'GCA
Cuts at: 0 343 1677
Size: 343 1334
HaeII r_GCGC'y
Cuts at: 0 171 642 733 1080 1323 1546 1677
Size: 171 471 91 347 243 223 131
Fragments arranged by size:
471 347 243 223 171 131 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1420 1452 1677
Size: 1045 32 343 32 225
Fragments arranged by size:
1045 343 225 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 764 1677
Size: 584 180 913
Fragments arranged by size:
913 584 180
MscI TGG'CCA
Cuts at: 0 364 736 916 1138 1677
Size: 364 372 180 222 539
Fragments arranged by size:
539 372 364 222 180
MslI CAynn'nnrTG
Cuts at: 0 298 949 1492 1677
Size: 298 651 543 185
Fragments arranged by size:
651 543 298 185
MspA1I CmG'CkG
Cuts at: 0 111 263 273 777 1426 1677
Size: 111 152 10 504 649 251
Fragments arranged by size:
649 504 251 152 111 10
NaeI GCC'GGC
Cuts at: 0 404 1677
Size: 404 1273
NgoMIV G'CCGG_C
Cuts at: 0 402 1677
Size: 402 1275
NruI TCG'CGA
Cuts at: 0 829 1677
Size: 829 848
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 1232 1677
Size: 63 1169 445
Fragments arranged by size:
1169 445 63
PfoI T'CCnGG_A
Cuts at: 0 9 56 176 1658 1677
Size: 9 47 120 1482 19
Fragments arranged by size:
1482 120 47 19 9
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 1006 1038 1474 1506 1677
Size: 850 32 124 32 436 32 171
Fragments arranged by size:
850 436 171 124 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 60 552 1068 1560 1662 1677
Size: 60 492 516 492 102 15
Fragments arranged by size:
516 492 492 102 60 15
PspOMI G'GGCC_C
Cuts at: 0 67 131 1677
Size: 67 64 1546
Fragments arranged by size:
1546 67 64
PstI C_TGCA'G
Cuts at: 0 98 620 911 1121 1307 1677
Size: 98 522 291 210 186 370
Fragments arranged by size:
522 370 291 210 186 98
PvuII CAG'CTG
Cuts at: 0 111 263 1677
Size: 111 152 1414
Fragments arranged by size:
1414 152 111
SanDI GG'GwC_CC
Cuts at: 0 1068 1677
Size: 1068 609
SbfI CC_TGCA'GG
Cuts at: 0 620 1677
Size: 620 1057
ScaI AGT'ACT
Cuts at: 0 568 1677
Size: 568 1109
SexAI A'CCwGG_T
Cuts at: 0 554 1677
Size: 554 1123
SfcI C'TryA_G
Cuts at: 0 94 429 616 907 1117 1303 1677
Size: 94 335 187 291 210 186 374
Fragments arranged by size:
374 335 291 210 187 186 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 412 1677
Size: 412 1265
SmaI CCC'GGG
Cuts at: 0 679 1677
Size: 679 998
SmlI C'TyrA_G
Cuts at: 0 742 1677
Size: 742 935
StuI AGG'CCT
Cuts at: 0 463 1648 1677
Size: 463 1185 29
Fragments arranged by size:
1185 463 29
StyI C'CwwG_G
Cuts at: 0 71 1677
Size: 71 1606
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1320 1495 1677
Size: 1320 175 182
Fragments arranged by size:
1320 182 175
TatI w'GTAC_w
Cuts at: 0 218 566 990 1677
Size: 218 348 424 687
Fragments arranged by size:
687 424 348 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1396 1428 1677
Size: 1396 32 249
Fragments arranged by size:
1396 249 32
Tth111I GACn'n_nGTC
Cuts at: 0 837 1482 1677
Size: 837 645 195
Fragments arranged by size:
837 645 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 1677
Size: 60 1617
XmaI C'CCGG_G
Cuts at: 0 677 1677
Size: 677 1000
XmnI GAAnn'nnTTC
Cuts at: 0 865 1677
Size: 865 812
ZraI GAC'GTC
Cuts at: 0 690 1677
Size: 690 987
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AleI AlfI AloI
AlwNI ApaI AscI AvaI BanI BanII BbsI BcgI
BciVI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NruI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI
SbfI ScaI SexAI SfcI SfiI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsiHKAI BsiWI BsmBI BspEI BspHI BssSI BstBI BstEII
BstZ17I CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NsiI
NspI PacI PasI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI