Restriction map for
pF1KA1181
(Linear) (Six-base) MAPSORT of: KIAA1181.seq Check: 3453 from: 1 to: 870
KIAA1181.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 212 870
Size: 212 658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 870
Size: 586 284
AflIII A'CryG_T
Cuts at: 0 392 870
Size: 392 478
AlwNI CAG_nnn'CTG
Cuts at: 0 744 832 870
Size: 744 88 38
Fragments arranged by size:
744 88 38
AvaI C'yCGr_G
Cuts at: 0 511 870
Size: 511 359
BanI G'GyrC_C
Cuts at: 0 39 870
Size: 39 831
BanII G_rGCy'C
Cuts at: 0 131 167 870
Size: 131 36 703
Fragments arranged by size:
703 131 36
BbvCI CC'TCA_GC
Cuts at: 0 703 870
Size: 703 167
BfrBI ATG'CAT
Cuts at: 0 818 867 870
Size: 818 49 3
Fragments arranged by size:
818 49 3
BglI GCCn_nnn'nGGC
Cuts at: 0 665 835 870
Size: 665 170 35
Fragments arranged by size:
665 170 35
Bme1580I G_kGCm'C
Cuts at: 0 44 578 870
Size: 44 534 292
Fragments arranged by size:
534 292 44
Bpu10I CC'TnA_GC
Cuts at: 0 703 870
Size: 703 167
BsaAI yAC'GTr
Cuts at: 0 393 870
Size: 393 477
BsaHI Gr'CG_yC
Cuts at: 0 209 870
Size: 209 661
BsaWI w'CCGG_w
Cuts at: 0 133 870
Size: 133 737
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 870
Size: 442 30 398
Fragments arranged by size:
442 398 30
BseYI C'CCAG_C
Cuts at: 0 416 855 870
Size: 416 439 15
Fragments arranged by size:
439 416 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 870
Size: 223 647
BsiHKAI G_wGCw'C
Cuts at: 0 131 167 870
Size: 131 36 703
Fragments arranged by size:
703 131 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 726 870
Size: 726 144
Bsp1286I G_dGCh'C
Cuts at: 0 44 131 167 578 870
Size: 44 87 36 411 292
Fragments arranged by size:
411 292 87 44 36
BsrFI r'CCGG_y
Cuts at: 0 799 870
Size: 799 71
BsrGI T'GTAC_A
Cuts at: 0 746 870
Size: 746 124
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 870
Size: 543 327
BstYI r'GATC_y
Cuts at: 0 317 848 870
Size: 317 531 22
Fragments arranged by size:
531 317 22
BtgI C'CryG_G
Cuts at: 0 492 578 870
Size: 492 86 292
Fragments arranged by size:
492 292 86
BtsI GCAGTG_nn'
Cuts at: 0 240 870
Size: 240 630
DraIII CAC_nnn'GTG
Cuts at: 0 291 406 870
Size: 291 115 464
Fragments arranged by size:
464 291 115
EaeI y'GGCC_r
Cuts at: 0 632 870
Size: 632 238
EarI CTCTTCn'nnn_
Cuts at: 0 106 115 121 870
Size: 106 9 6 749
Fragments arranged by size:
749 106 9 6
EcoICRI GAG'CTC
Cuts at: 0 129 165 870
Size: 129 36 705
Fragments arranged by size:
705 129 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 870
Size: 586 284
EcoO109I rG'GnC_Cy
Cuts at: 0 48 375 785 870
Size: 48 327 410 85
Fragments arranged by size:
410 327 85 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 870
Size: 477 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 870
Size: 560 310
MscI TGG'CCA
Cuts at: 0 634 870
Size: 634 236
MspA1I CmG'CkG
Cuts at: 0 194 324 609 744 855 870
Size: 194 130 285 135 111 15
Fragments arranged by size:
285 194 135 130 111 15
NaeI GCC'GGC
Cuts at: 0 801 870
Size: 801 69
NgoMIV G'CCGG_C
Cuts at: 0 799 870
Size: 799 71
NsiI A_TGCA'T
Cuts at: 0 820 869 870
Size: 820 49 1
Fragments arranged by size:
820 49 1
NspI r_CATG'y
Cuts at: 0 445 870
Size: 445 425
PfoI T'CCnGG_A
Cuts at: 0 806 870
Size: 806 64
PmlI CAC'GTG
Cuts at: 0 393 870
Size: 393 477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 870
Size: 343 32 495
Fragments arranged by size:
495 343 32
PpuMI rG'GwC_Cy
Cuts at: 0 48 375 785 870
Size: 48 327 410 85
Fragments arranged by size:
410 327 85 48
PshAI GACnn'nnGTC
Cuts at: 0 212 442 870
Size: 212 230 428
Fragments arranged by size:
428 230 212
PsiI TTA'TAA
Cuts at: 0 142 870
Size: 142 728
PvuII CAG'CTG
Cuts at: 0 855 870
Size: 855 15
SacI G_AGCT'C
Cuts at: 0 131 167 870
Size: 131 36 703
Fragments arranged by size:
703 131 36
SfcI C'TryA_G
Cuts at: 0 30 475 654 870
Size: 30 445 179 216
Fragments arranged by size:
445 216 179 30
StuI AGG'CCT
Cuts at: 0 838 870
Size: 838 32
StyI C'CwwG_G
Cuts at: 0 44 870
Size: 44 826
TatI w'GTAC_w
Cuts at: 0 623 722 746 870
Size: 623 99 24 124
Fragments arranged by size:
623 124 99 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 870
Size: 442 32 396
Fragments arranged by size:
442 396 32
ZraI GAC'GTC
Cuts at: 0 210 870
Size: 210 660
Enzymes that do cut:
AatII AcuI AflIII AlwNI AvaI BanI BanII BbvCI
BfrBI BglI Bme1580I Bpu10I BsaAI BsaHI BsaWI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BstXI
BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I FalI MmeI MscI MspA1I NaeI NgoMIV NsiI
NspI PfoI PmlI PpiI PpuMI PshAI PsiI PvuII
SacI SfcI StuI StyI TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BglII BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BseRI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NcoI NdeI NheI NotI NruI
PacI PasI PciI PflMI PmeI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI