Restriction map for
pF1KA1190
(Linear) (Six-base) MAPSORT of: KIAA1190.seq Check: 5030 from: 1 to: 2241
KIAA1190.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1654 2183 2241
Size: 1654 529 58
Fragments arranged by size:
1654 529 58
Acc65I G'GTAC_C
Cuts at: 0 1165 1346 2241
Size: 1165 181 895
Fragments arranged by size:
1165 895 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 2241
Size: 1199 1042
AfeI AGC'GCT
Cuts at: 0 1411 1750 2002 2241
Size: 1411 339 252 239
Fragments arranged by size:
1411 339 252 239
AflIII A'CryG_T
Cuts at: 0 737 1480 1988 2241
Size: 737 743 508 253
Fragments arranged by size:
743 737 508 253
AleI CACnn'nnGTG
Cuts at: 0 95 2241
Size: 95 2146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1634 2241
Size: 1634 607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1708 1740 2241
Size: 1708 32 501
Fragments arranged by size:
1708 501 32
AlwNI CAG_nnn'CTG
Cuts at: 0 517 535 732 1261 2157 2241
Size: 517 18 197 529 896 84
Fragments arranged by size:
896 529 517 197 84 18
ApaI G_GGCC'C
Cuts at: 0 478 608 2241
Size: 478 130 1633
Fragments arranged by size:
1633 478 130
ApaLI G'TGCA_C
Cuts at: 0 1534 1693 2241
Size: 1534 159 548
Fragments arranged by size:
1534 548 159
AvaI C'yCGr_G
Cuts at: 0 1142 1244 2139 2241
Size: 1142 102 895 102
Fragments arranged by size:
1142 895 102 102
BanI G'GyrC_C
Cuts at: 0 63 186 670 766 1157 1165 1346 1946
Size: 63 123 484 96 391 8 181 600
Cuts at: 1946 2241
Size: 295
Fragments arranged by size:
600 484 391 295 181 123 96 63
8
BanII G_rGCy'C
Cuts at: 0 478 608 1033 2241
Size: 478 130 425 1208
Fragments arranged by size:
1208 478 425 130
BbeI G_GCGC'C
Cuts at: 0 1161 2241
Size: 1161 1080
BbsI GAAGACnn'nnnn_
Cuts at: 0 1876 2241
Size: 1876 365
BglI GCCn_nnn'nGGC
Cuts at: 0 379 2241
Size: 379 1862
BglII A'GATC_T
Cuts at: 0 458 1388 1568 1904 2241
Size: 458 930 180 336 337
Fragments arranged by size:
930 458 337 336 180
BlpI GC'TnA_GC
Cuts at: 0 268 2241
Size: 268 1973
Bme1580I G_kGCm'C
Cuts at: 0 68 361 478 608 965 1324 1538 1697
Size: 68 293 117 130 357 359 214 159
Cuts at: 1697 2241
Size: 544
Fragments arranged by size:
544 359 357 293 214 159 130 117
68
BmgBI CAC'GTC
Cuts at: 0 48 1989 2241
Size: 48 1941 252
Fragments arranged by size:
1941 252 48
BmtI G_CTAG'C
Cuts at: 0 106 2241
Size: 106 2135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 380 649 1244 1309 1372 1441 2241
Size: 196 184 269 595 65 63 69 800
Fragments arranged by size:
800 595 269 196 184 69 65 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 2008 2241
Size: 1672 336 233
Fragments arranged by size:
1672 336 233
BsaI GGTCTCn'nnnn_
Cuts at: 0 1645 2241
Size: 1645 596
BsaAI yAC'GTr
Cuts at: 0 738 1602 2241
Size: 738 864 639
Fragments arranged by size:
864 738 639
BsaHI Gr'CG_yC
Cuts at: 0 329 1158 2078 2241
Size: 329 829 920 163
Fragments arranged by size:
920 829 329 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1708 1738 2241
Size: 1708 30 503
Fragments arranged by size:
1708 503 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 607 847 850 853 865 868 871
Size: 586 21 240 3 3 12 3 3
Cuts at: 871 874 916 919 922 925 964 967 1000
Size: 3 42 3 3 3 39 3 33
Cuts at: 1000 1003 1012 1051 1054 1057 1066 1306 2241
Size: 3 9 39 3 3 9 240 935
Fragments arranged by size:
935 586 240 240 42 39 39 33
21 12 9 9 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 68 374 554 609 780 1063 1186 1269
Size: 68 306 180 55 171 283 123 83
Cuts at: 1269 2108 2241
Size: 839 133
Fragments arranged by size:
839 306 283 180 171 133 123 83
68 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 718 1423 2241
Size: 375 343 705 818
Fragments arranged by size:
818 705 375 343
BsiHKAI G_wGCw'C
Cuts at: 0 269 1033 1538 1697 1865 2241
Size: 269 764 505 159 168 376
Fragments arranged by size:
764 505 376 269 168 159
Bsp1286I G_dGCh'C
Cuts at: 0 68 269 361 478 608 965 1033 1324
Size: 68 201 92 117 130 357 68 291
Cuts at: 1324 1538 1697 1865 2241
Size: 214 159 168 376
Fragments arranged by size:
376 357 291 214 201 168 159 130
117 92 68 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1654 1663 2183 2241
Size: 1654 9 520 58
Fragments arranged by size:
1654 520 58 9
BsrBI CCG'CTC
Cuts at: 0 335 2193 2241
Size: 335 1858 48
Fragments arranged by size:
1858 335 48
BsrDI GCAATG_nn'
Cuts at: 0 280 1749 2241
Size: 280 1469 492
Fragments arranged by size:
1469 492 280
BsrFI r'CCGG_y
Cuts at: 0 378 598 1928 1949 2068 2241
Size: 378 220 1330 21 119 173
Fragments arranged by size:
1330 378 220 173 119 21
BssSI C'ACGA_G
Cuts at: 0 259 1325 2241
Size: 259 1066 916
Fragments arranged by size:
1066 916 259
BstAPI GCAn_nnn'nTGC
Cuts at: 0 517 1701 2127 2241
Size: 517 1184 426 114
Fragments arranged by size:
1184 517 426 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 2241
Size: 237 2004
BstYI r'GATC_y
Cuts at: 0 206 458 1388 1568 1904 2241
Size: 206 252 930 180 336 337
Fragments arranged by size:
930 337 336 252 206 180
BtgI C'CryG_G
Cuts at: 0 743 788 1081 1592 2241
Size: 743 45 293 511 649
Fragments arranged by size:
743 649 511 293 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 285 2241
Size: 285 1956
BtsI GCAGTG_nn'
Cuts at: 0 730 897 1699 2125 2204 2241
Size: 730 167 802 426 79 37
Fragments arranged by size:
802 730 426 167 79 37
DraIII CAC_nnn'GTG
Cuts at: 0 193 264 2241
Size: 193 71 1977
Fragments arranged by size:
1977 193 71
EaeI y'GGCC_r
Cuts at: 0 248 1130 1797 1995 2066 2241
Size: 248 882 667 198 71 175
Fragments arranged by size:
882 667 248 198 175 71
EarI CTCTTCn'nnn_
Cuts at: 0 31 139 635 647 863 869 911 932
Size: 31 108 496 12 216 6 42 21
Cuts at: 932 941 971 977 989 1198 2200 2241
Size: 9 30 6 12 209 1002 41
Fragments arranged by size:
1002 496 216 209 108 42 41 31
30 21 12 12 9 6 6
EcoICRI GAG'CTC
Cuts at: 0 1031 2241
Size: 1031 1210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 380 649 1199 1244 1309 1372 1441
Size: 196 184 269 550 45 65 63 69
Cuts at: 1441 2241
Size: 800
Fragments arranged by size:
800 550 269 196 184 69 65 63
45
EcoO109I rG'GnC_Cy
Cuts at: 0 474 475 605 1006 2241
Size: 474 1 130 401 1235
Fragments arranged by size:
1235 474 401 130 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1869 2241
Size: 1869 372
HaeII r_GCGC'y
Cuts at: 0 24 275 1161 1413 1752 2004 2241
Size: 24 251 886 252 339 252 237
Fragments arranged by size:
886 339 252 252 251 237 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 472 504 2241
Size: 319 32 121 32 1737
Fragments arranged by size:
1737 319 121 32 32
HincII GTy'rAC
Cuts at: 0 243 2241
Size: 243 1998
HindIII A'AGCT_T
Cuts at: 0 1490 1501 2241
Size: 1490 11 740
Fragments arranged by size:
1490 740 11
KasI G'GCGC_C
Cuts at: 0 1157 2241
Size: 1157 1084
KpnI G_GTAC'C
Cuts at: 0 1169 1350 2241
Size: 1169 181 891
Fragments arranged by size:
1169 891 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 2039 2241
Size: 626 1413 202
Fragments arranged by size:
1413 626 202
MscI TGG'CCA
Cuts at: 0 1132 1799 1997 2241
Size: 1132 667 198 244
Fragments arranged by size:
1132 667 244 198
MslI CAynn'nnrTG
Cuts at: 0 95 1364 1366 2241
Size: 95 1269 2 875
Fragments arranged by size:
1269 875 95 2
MspA1I CmG'CkG
Cuts at: 0 153 389 400 1343 1770 2086 2241
Size: 153 236 11 943 427 316 155
Fragments arranged by size:
943 427 316 236 155 153 11
NaeI GCC'GGC
Cuts at: 0 380 1930 2070 2241
Size: 380 1550 140 171
Fragments arranged by size:
1550 380 171 140
NarI GG'CG_CC
Cuts at: 0 1158 2241
Size: 1158 1083
NcoI C'CATG_G
Cuts at: 0 1592 2241
Size: 1592 649
NgoMIV G'CCGG_C
Cuts at: 0 378 1928 2068 2241
Size: 378 1550 140 173
Fragments arranged by size:
1550 378 173 140
NheI G'CTAG_C
Cuts at: 0 102 2241
Size: 102 2139
NspI r_CATG'y
Cuts at: 0 1386 1484 1638 1683 2022 2241
Size: 1386 98 154 45 339 219
Fragments arranged by size:
1386 339 219 154 98 45
PciI A'CATG_T
Cuts at: 0 1480 2241
Size: 1480 761
PflMI CCAn_nnn'nTGG
Cuts at: 0 1222 2064 2241
Size: 1222 842 177
Fragments arranged by size:
1222 842 177
PmlI CAC'GTG
Cuts at: 0 738 1602 2241
Size: 738 864 639
Fragments arranged by size:
864 738 639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1540 1567 1572 1599 1708 1740 2241
Size: 1540 27 5 27 109 32 501
Fragments arranged by size:
1540 501 109 32 27 27 5
PspOMI G'GGCC_C
Cuts at: 0 474 604 2241
Size: 474 130 1637
Fragments arranged by size:
1637 474 130
PstI C_TGCA'G
Cuts at: 0 158 203 290 818 1706 2241
Size: 158 45 87 528 888 535
Fragments arranged by size:
888 535 528 158 87 45
PvuII CAG'CTG
Cuts at: 0 153 389 400 1770 2241
Size: 153 236 11 1370 471
Fragments arranged by size:
1370 471 236 153 11
SacI G_AGCT'C
Cuts at: 0 1033 2241
Size: 1033 1208
SapI GCTCTTCn'nnn_
Cuts at: 0 647 2200 2241
Size: 647 1553 41
Fragments arranged by size:
1553 647 41
ScaI AGT'ACT
Cuts at: 0 1852 2241
Size: 1852 389
SexAI A'CCwGG_T
Cuts at: 0 189 348 2241
Size: 189 159 1893
Fragments arranged by size:
1893 189 159
SfcI C'TryA_G
Cuts at: 0 111 154 199 286 675 814 1702 2241
Size: 111 43 45 87 389 139 888 539
Fragments arranged by size:
888 539 389 139 111 87 45 43
SfoI GGC'GCC
Cuts at: 0 1159 2241
Size: 1159 1082
SgrAI Cr'CCGG_yG
Cuts at: 0 1949 2241
Size: 1949 292
SmaI CCC'GGG
Cuts at: 0 1144 2141 2241
Size: 1144 997 100
Fragments arranged by size:
1144 997 100
SmlI C'TyrA_G
Cuts at: 0 1687 2023 2241
Size: 1687 336 218
Fragments arranged by size:
1687 336 218
SphI G_CATG'C
Cuts at: 0 1386 2241
Size: 1386 855
StuI AGG'CCT
Cuts at: 0 449 2096 2241
Size: 449 1647 145
Fragments arranged by size:
1647 449 145
StyI C'CwwG_G
Cuts at: 0 1277 1592 1628 2241
Size: 1277 315 36 613
Fragments arranged by size:
1277 613 315 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 2241
Size: 154 2087
TatI w'GTAC_w
Cuts at: 0 1757 1850 2241
Size: 1757 93 391
Fragments arranged by size:
1757 391 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 507 539 750 782 2241
Size: 507 32 211 32 1459
Fragments arranged by size:
1459 507 211 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2077 2241
Size: 2077 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1266 1284 2241
Size: 792 474 18 957
Fragments arranged by size:
957 792 474 18
XmaI C'CCGG_G
Cuts at: 0 1142 2139 2241
Size: 1142 997 102
Fragments arranged by size:
1142 997 102
XmnI GAAnn'nnTTC
Cuts at: 0 1555 1582 1723 2044 2241
Size: 1555 27 141 321 197
Fragments arranged by size:
1555 321 197 141 27
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BglI BglII BlpI Bme1580I BmgBI BmtI BpmI BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PmlI PpiI PspOMI
PstI PvuII SacI SapI ScaI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BmrI BplI Bpu10I BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI
MfeI MluI NdeI NotI NruI NsiI PacI PasI
PfoI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SgfI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI ZraI