Restriction map for
pF1KSDA1197
(Linear) (Six-base) MAPSORT of: KIAA1197.seq Check: 317 from: 1 to: 4266
KIAA1197.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1512 3264 4266
Size: 1512 1752 1002
Fragments arranged by size:
1752 1512 1002
Acc65I G'GTAC_C
Cuts at: 0 1956 4266
Size: 1956 2310
AclI AA'CG_TT
Cuts at: 0 2215 4266
Size: 2215 2051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1187 2288 2936 4102 4266
Size: 940 247 1101 648 1166 164
Fragments arranged by size:
1166 1101 940 648 247 164
AflIII A'CryG_T
Cuts at: 0 301 4049 4266
Size: 301 3748 217
Fragments arranged by size:
3748 301 217
AleI CACnn'nnGTG
Cuts at: 0 1762 4266
Size: 1762 2504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1314 1484 4266
Size: 1314 170 2782
Fragments arranged by size:
2782 1314 170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3940 3972 4266
Size: 3940 32 294
Fragments arranged by size:
3940 294 32
AlwNI CAG_nnn'CTG
Cuts at: 0 201 1816 2022 2538 3313 3982 4266
Size: 201 1615 206 516 775 669 284
Fragments arranged by size:
1615 775 669 516 284 206 201
ApoI r'AATT_y
Cuts at: 0 93 340 1619 2253 3985 4266
Size: 93 247 1279 634 1732 281
Fragments arranged by size:
1732 1279 634 281 247 93
AseI AT'TA_AT
Cuts at: 0 740 1484 3487 4266
Size: 740 744 2003 779
Fragments arranged by size:
2003 779 744 740
AvaI C'yCGr_G
Cuts at: 0 101 3781 3862 4266
Size: 101 3680 81 404
Fragments arranged by size:
3680 404 101 81
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 299 332 1124 1157 4266
Size: 299 33 792 33 3109
Fragments arranged by size:
3109 792 299 33 33
BamHI G'GATC_C
Cuts at: 0 2191 4266
Size: 2191 2075
BanI G'GyrC_C
Cuts at: 0 1956 2404 3686 4266
Size: 1956 448 1282 580
Fragments arranged by size:
1956 1282 580 448
BanII G_rGCy'C
Cuts at: 0 3782 4266
Size: 3782 484
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1013 2794 2927 3334 4266
Size: 265 748 1781 133 407 932
Fragments arranged by size:
1781 932 748 407 265 133
BbvCI CC'TCA_GC
Cuts at: 0 3889 4266
Size: 3889 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3192 3226 4266
Size: 3192 34 1040
Fragments arranged by size:
3192 1040 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 531 3102 4129 4266
Size: 531 2571 1027 137
Fragments arranged by size:
2571 1027 531 137
BclI T'GATC_A
Cuts at: 0 1766 4266
Size: 1766 2500
BglI GCCn_nnn'nGGC
Cuts at: 0 2248 4266
Size: 2248 2018
BglII A'GATC_T
Cuts at: 0 1568 4266
Size: 1568 2698
BlpI GC'TnA_GC
Cuts at: 0 2132 2382 4266
Size: 2132 250 1884
Fragments arranged by size:
2132 1884 250
Bme1580I G_kGCm'C
Cuts at: 0 1805 2581 3790 4266
Size: 1805 776 1209 476
Fragments arranged by size:
1805 1209 776 476
BmgBI CAC'GTC
Cuts at: 0 3047 3756 4266
Size: 3047 709 510
Fragments arranged by size:
3047 709 510
BmrI ACTGGGnnnn_n'
Cuts at: 0 1078 1538 1914 2642 2665 4266
Size: 1078 460 376 728 23 1601
Fragments arranged by size:
1601 1078 728 460 376 23
BmtI G_CTAG'C
Cuts at: 0 1163 4266
Size: 1163 3103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1925 2356 2540 3099 3209 3269 3290
Size: 1430 495 431 184 559 110 60 21
Cuts at: 3290 3646 3850 4266
Size: 356 204 416
Fragments arranged by size:
1430 559 495 431 416 356 204 184
110 60 21
Bpu10I CC'TnA_GC
Cuts at: 0 3889 4266
Size: 3889 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2318 2545 4266
Size: 2318 227 1721
Fragments arranged by size:
2318 1721 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 507 1593 4266
Size: 507 1086 2673
Fragments arranged by size:
2673 1086 507
BsaAI yAC'GTr
Cuts at: 0 1455 1800 4266
Size: 1455 345 2466
Fragments arranged by size:
2466 1455 345
BsaBI GATnn'nnATC
Cuts at: 0 904 3916 4015 4266
Size: 904 3012 99 251
Fragments arranged by size:
3012 904 251 99
BsaWI w'CCGG_w
Cuts at: 0 660 3094 3711 4266
Size: 660 2434 617 555
Fragments arranged by size:
2434 660 617 555
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1428 1458 1555 1585 4266
Size: 1428 30 97 30 2681
Fragments arranged by size:
2681 1428 97 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2426 2429 2432 2435 2438 2441 2444 2447
Size: 2426 3 3 3 3 3 3 3
Cuts at: 2447 2486 2489 2492 2507 3792 3886 3889 4266
Size: 39 3 3 15 1285 94 3 377
Fragments arranged by size:
2426 1285 377 94 39 15 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 733 842 1155 3154 3295 3408 4266
Size: 733 109 313 1999 141 113 858
Fragments arranged by size:
1999 858 733 313 141 113 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 2161 2660 2888 3217 4266
Size: 136 2025 499 228 329 1049
Fragments arranged by size:
2025 1049 499 329 228 136
BsmI GAATG_Cn'
Cuts at: 0 585 4266
Size: 585 3681
BsmBI CGTCTCn'nnnn_
Cuts at: 0 958 4266
Size: 958 3308
Bsp1286I G_dGCh'C
Cuts at: 0 1805 2581 3782 3790 4266
Size: 1805 776 1201 8 476
Fragments arranged by size:
1805 1201 776 476 8
BspEI T'CCGG_A
Cuts at: 0 660 3094 4266
Size: 660 2434 1172
Fragments arranged by size:
2434 1172 660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 259 1512 3019 3264 3845 3971 4266
Size: 259 1253 1507 245 581 126 295
Fragments arranged by size:
1507 1253 581 295 259 245 126
BsrDI GCAATG_nn'
Cuts at: 0 645 1420 3599 4266
Size: 645 775 2179 667
Fragments arranged by size:
2179 775 667 645
BsrFI r'CCGG_y
Cuts at: 0 1471 4266
Size: 1471 2795
BssSI C'ACGA_G
Cuts at: 0 3052 4266
Size: 3052 1214
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3316 4266
Size: 3316 950
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2238 2614 4266
Size: 2238 376 1652
Fragments arranged by size:
2238 1652 376
BstYI r'GATC_y
Cuts at: 0 1568 2018 2191 4266
Size: 1568 450 173 2075
Fragments arranged by size:
2075 1568 450 173
Bsu36I CC'TnA_GG
Cuts at: 0 1712 3727 4266
Size: 1712 2015 539
Fragments arranged by size:
2015 1712 539
BtgI C'CryG_G
Cuts at: 0 1263 1574 2742 2879 3002 4031 4061 4266
Size: 1263 311 1168 137 123 1029 30 205
Fragments arranged by size:
1263 1168 1029 311 205 137 123 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 973 3660 4266
Size: 973 2687 606
Fragments arranged by size:
2687 973 606
BtsI GCAGTG_nn'
Cuts at: 0 1466 2448 3389 3491 4266
Size: 1466 982 941 102 775
Fragments arranged by size:
1466 982 941 775 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 614 649 3515 3550 4266
Size: 614 35 2866 35 716
Fragments arranged by size:
2866 716 614 35 35
EaeI y'GGCC_r
Cuts at: 0 2013 2240 2592 4266
Size: 2013 227 352 1674
Fragments arranged by size:
2013 1674 352 227
EarI CTCTTCn'nnn_
Cuts at: 0 831 2046 2179 3929 4266
Size: 831 1215 133 1750 337
Fragments arranged by size:
1750 1215 831 337 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1187 1430 1925 2288 2356 2540 2936
Size: 940 247 243 495 363 68 184 396
Cuts at: 2936 3099 3209 3269 3290 3646 3850 4102 4266
Size: 163 110 60 21 356 204 252 164
Fragments arranged by size:
940 495 396 363 356 252 247 243
204 184 164 163 110 68 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1736 1907 3364 4117 4266
Size: 1736 171 1457 753 149
Fragments arranged by size:
1736 1457 753 171 149
EcoO109I rG'GnC_Cy
Cuts at: 0 719 3834 4266
Size: 719 3115 432
Fragments arranged by size:
3115 719 432
EcoRI G'AATT_C
Cuts at: 0 93 4266
Size: 93 4173
EcoRV GAT'ATC
Cuts at: 0 906 2528 4110 4266
Size: 906 1622 1582 156
Fragments arranged by size:
1622 1582 906 156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 440 557 4266
Size: 440 117 3709
Fragments arranged by size:
3709 440 117
HaeII r_GCGC'y
Cuts at: 0 3044 4266
Size: 3044 1222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 412 433 444 465 1428 1460
Size: 37 32 343 21 11 21 963 32
Cuts at: 1460 2840 2872 3727 3759 3775 3807 4134 4166
Size: 1380 32 855 32 16 32 327 32
Cuts at: 4166 4266
Size: 100
Fragments arranged by size:
1380 963 855 343 327 100 37 32
32 32 32 32 32 21 21 16
11
HincII GTy'rAC
Cuts at: 0 689 4266
Size: 689 3577
HindIII A'AGCT_T
Cuts at: 0 1091 4266
Size: 1091 3175
KpnI G_GTAC'C
Cuts at: 0 1960 4266
Size: 1960 2306
MfeI C'AATT_G
Cuts at: 0 86 1697 2099 4266
Size: 86 1611 402 2167
Fragments arranged by size:
2167 1611 402 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4113 4266
Size: 4113 153
MscI TGG'CCA
Cuts at: 0 2015 2242 2594 4266
Size: 2015 227 352 1672
Fragments arranged by size:
2015 1672 352 227
MslI CAynn'nnrTG
Cuts at: 0 1228 1366 1762 4266
Size: 1228 138 396 2504
Fragments arranged by size:
2504 1228 396 138
MspA1I CmG'CkG
Cuts at: 0 576 2173 2386 2450 3313 3515 3577 3692
Size: 576 1597 213 64 863 202 62 115
Cuts at: 3692 4098 4266
Size: 406 168
Fragments arranged by size:
1597 863 576 406 213 202 168 115
64 62
NaeI GCC'GGC
Cuts at: 0 1473 4266
Size: 1473 2793
NcoI C'CATG_G
Cuts at: 0 1263 2742 4266
Size: 1263 1479 1524
Fragments arranged by size:
1524 1479 1263
NgoMIV G'CCGG_C
Cuts at: 0 1471 4266
Size: 1471 2795
NheI G'CTAG_C
Cuts at: 0 1159 4266
Size: 1159 3107
NspI r_CATG'y
Cuts at: 0 305 4266
Size: 305 3961
PasI CC'CwG_GG
Cuts at: 0 2196 2574 3976 4266
Size: 2196 378 1402 290
Fragments arranged by size:
2196 1402 378 290
PciI A'CATG_T
Cuts at: 0 301 4266
Size: 301 3965
PflMI CCAn_nnn'nTGG
Cuts at: 0 2022 4266
Size: 2022 2244
PfoI T'CCnGG_A
Cuts at: 0 1902 3623 4266
Size: 1902 1721 643
Fragments arranged by size:
1902 1721 643
PmlI CAC'GTG
Cuts at: 0 1455 1800 4266
Size: 1455 345 2466
Fragments arranged by size:
2466 1455 345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 854 886 4266
Size: 854 32 3380
Fragments arranged by size:
3380 854 32
PpuMI rG'GwC_Cy
Cuts at: 0 719 3834 4266
Size: 719 3115 432
Fragments arranged by size:
3115 719 432
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2490 2522 4266
Size: 2490 32 1744
Fragments arranged by size:
2490 1744 32
PstI C_TGCA'G
Cuts at: 0 270 3468 4028 4266
Size: 270 3198 560 238
Fragments arranged by size:
3198 560 270 238
PvuII CAG'CTG
Cuts at: 0 2386 3313 3515 4098 4266
Size: 2386 927 202 583 168
Fragments arranged by size:
2386 927 583 202 168
SanDI GG'GwC_CC
Cuts at: 0 719 4266
Size: 719 3547
SapI GCTCTTCn'nnn_
Cuts at: 0 2046 4266
Size: 2046 2220
ScaI AGT'ACT
Cuts at: 0 2508 3544 4266
Size: 2508 1036 722
Fragments arranged by size:
2508 1036 722
SexAI A'CCwGG_T
Cuts at: 0 1782 4266
Size: 1782 2484
SfcI C'TryA_G
Cuts at: 0 266 3269 3464 4024 4088 4266
Size: 266 3003 195 560 64 178
Fragments arranged by size:
3003 560 266 195 178 64
SmlI C'TyrA_G
Cuts at: 0 101 2333 2560 4266
Size: 101 2232 227 1706
Fragments arranged by size:
2232 1706 227 101
SspI AAT'ATT
Cuts at: 0 4188 4250 4266
Size: 4188 62 16
Fragments arranged by size:
4188 62 16
StyI C'CwwG_G
Cuts at: 0 1263 1593 1943 2030 2082 2153 2646 2742
Size: 1263 330 350 87 52 71 493 96
Cuts at: 2742 4266
Size: 1524
Fragments arranged by size:
1524 1263 493 350 330 96 87 71
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1236 3366 4266
Size: 1236 2130 900
Fragments arranged by size:
2130 1236 900
TatI w'GTAC_w
Cuts at: 0 1546 2506 3542 4266
Size: 1546 960 1036 724
Fragments arranged by size:
1546 1036 960 724
XbaI T'CTAG_A
Cuts at: 0 544 4266
Size: 544 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2637 4266
Size: 2637 1629
XhoI C'TCGA_G
Cuts at: 0 101 4266
Size: 101 4165
XmnI GAAnn'nnTTC
Cuts at: 0 1150 1602 4266
Size: 1150 452 2664
Fragments arranged by size:
2664 1150 452
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PsrI PstI PvuII
SanDI SapI ScaI SexAI SfcI SmlI SspI StyI
TaqII TatI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AsiSI AvrII BbeI BfrBI BplI BsaHI BsiEI
BsiHKAI BsiWI BspHI BsrBI BsrGI BssHII BstBI BstEII
BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoICRI
FseI FspI FspAI HpaI KasI MluI NarI NdeI
NotI NruI NsiI PacI PmeI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XmaI ZraI