Restriction map for
pF1KA1198
(Linear) (Six-base) MAPSORT of: KIAA1198.seq Check: 2615 from: 1 to: 1587
KIAA1198.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 1512 1575 1587
Size: 1512 63 12
Fragments arranged by size:
1512 63 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1198 1356 1515 1587
Size: 927 271 158 159 72
Fragments arranged by size:
927 271 159 158 72
AflIII A'CryG_T
Cuts at: 0 1053 1087 1587
Size: 1053 34 500
Fragments arranged by size:
1053 500 34
AleI CACnn'nnGTG
Cuts at: 0 1408 1587
Size: 1408 179
ApaLI G'TGCA_C
Cuts at: 0 1135 1555 1587
Size: 1135 420 32
Fragments arranged by size:
1135 420 32
ApoI r'AATT_y
Cuts at: 0 9 641 725 1458 1481 1587
Size: 9 632 84 733 23 106
Fragments arranged by size:
733 632 106 84 23 9
AvaI C'yCGr_G
Cuts at: 0 43 1587
Size: 43 1544
BamHI G'GATC_C
Cuts at: 0 222 1587
Size: 222 1365
BanI G'GyrC_C
Cuts at: 0 797 1587
Size: 797 790
BbeI G_GCGC'C
Cuts at: 0 801 1587
Size: 801 786
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 1353 1587
Size: 483 870 234
Fragments arranged by size:
870 483 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 672 706 1587
Size: 672 34 881
Fragments arranged by size:
881 672 34
BfrBI ATG'CAT
Cuts at: 0 498 1587
Size: 498 1089
BglII A'GATC_T
Cuts at: 0 526 1587
Size: 526 1061
Bme1580I G_kGCm'C
Cuts at: 0 1139 1559 1587
Size: 1139 420 28
Fragments arranged by size:
1139 420 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 223 603 671 755 839 923 1007
Size: 97 126 380 68 84 84 84 84
Cuts at: 1007 1427 1511 1587
Size: 420 84 76
Fragments arranged by size:
420 380 126 97 84 84 84 84
84 76 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1023 1587
Size: 196 827 564
Fragments arranged by size:
827 564 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 510 1587
Size: 510 1077
BsaAI yAC'GTr
Cuts at: 0 956 1056 1554 1587
Size: 956 100 498 33
Fragments arranged by size:
956 498 100 33
BsaHI Gr'CG_yC
Cuts at: 0 798 1587
Size: 798 789
BsaWI w'CCGG_w
Cuts at: 0 1069 1587
Size: 1069 518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 1587
Size: 154 30 1403
Fragments arranged by size:
1403 154 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 220 1587
Size: 61 159 1367
Fragments arranged by size:
1367 159 61
BsiHKAI G_wGCw'C
Cuts at: 0 1139 1559 1587
Size: 1139 420 28
Fragments arranged by size:
1139 420 28
BsmI GAATG_Cn'
Cuts at: 0 772 1345 1587
Size: 772 573 242
Fragments arranged by size:
772 573 242
Bsp1286I G_dGCh'C
Cuts at: 0 1139 1559 1587
Size: 1139 420 28
Fragments arranged by size:
1139 420 28
BspHI T'CATG_A
Cuts at: 0 969 1587
Size: 969 618
BsrDI GCAATG_nn'
Cuts at: 0 934 1587
Size: 934 653
BstBI TT'CG_AA
Cuts at: 0 627 711 1047 1299 1383 1587
Size: 627 84 336 252 84 204
Fragments arranged by size:
627 336 252 204 84 84
BstYI r'GATC_y
Cuts at: 0 222 526 1587
Size: 222 304 1061
Fragments arranged by size:
1061 304 222
BtgI C'CryG_G
Cuts at: 0 135 1587
Size: 135 1452
BtsI GCAGTG_nn'
Cuts at: 0 480 785 1438 1587
Size: 480 305 653 149
Fragments arranged by size:
653 480 305 149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 576 611 1332 1367 1587
Size: 576 35 721 35 220
Fragments arranged by size:
721 576 220 35 35
DraI TTT'AAA
Cuts at: 0 1457 1587
Size: 1457 130
DraIII CAC_nnn'GTG
Cuts at: 0 373 1587
Size: 373 1214
EaeI y'GGCC_r
Cuts at: 0 228 1587
Size: 228 1359
EarI CTCTTCn'nnn_
Cuts at: 0 797 1587
Size: 797 790
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 223 603 671 755 839 923 927
Size: 97 126 380 68 84 84 84 4
Cuts at: 927 1007 1198 1356 1427 1511 1515 1587
Size: 80 191 158 71 84 4 72
Fragments arranged by size:
380 191 158 126 97 84 84 84
84 80 72 71 68 4 4
EcoRI G'AATT_C
Cuts at: 0 9 641 725 1481 1587
Size: 9 632 84 756 106
Fragments arranged by size:
756 632 106 84 9
EcoRV GAT'ATC
Cuts at: 0 781 1587
Size: 781 806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1297 1587
Size: 1297 290
FspI TGC'GCA
Cuts at: 0 4 1587
Size: 4 1583
HaeII r_GCGC'y
Cuts at: 0 801 1587
Size: 801 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 154 163 186 408 440 1587
Size: 131 23 9 23 222 32 1147
Fragments arranged by size:
1147 222 131 32 23 23 9
HindIII A'AGCT_T
Cuts at: 0 1442 1587
Size: 1442 145
KasI G'GCGC_C
Cuts at: 0 797 1587
Size: 797 790
MscI TGG'CCA
Cuts at: 0 230 1587
Size: 230 1357
MslI CAynn'nnrTG
Cuts at: 0 563 582 1408 1422 1587
Size: 563 19 826 14 165
Fragments arranged by size:
826 563 165 19 14
NarI GG'CG_CC
Cuts at: 0 798 1587
Size: 798 789
NcoI C'CATG_G
Cuts at: 0 135 1587
Size: 135 1452
NsiI A_TGCA'T
Cuts at: 0 500 1587
Size: 500 1087
NspI r_CATG'y
Cuts at: 0 1091 1587
Size: 1091 496
PciI A'CATG_T
Cuts at: 0 1087 1587
Size: 1087 500
PmlI CAC'GTG
Cuts at: 0 1056 1554 1587
Size: 1056 498 33
Fragments arranged by size:
1056 498 33
PspXI vC'TCGA_Gb
Cuts at: 0 43 1587
Size: 43 1544
PstI C_TGCA'G
Cuts at: 0 475 1587
Size: 475 1112
SapI GCTCTTCn'nnn_
Cuts at: 0 797 1587
Size: 797 790
SfcI C'TryA_G
Cuts at: 0 243 471 1587
Size: 243 228 1116
Fragments arranged by size:
1116 243 228
SfoI GGC'GCC
Cuts at: 0 799 1587
Size: 799 788
SmlI C'TyrA_G
Cuts at: 0 43 175 1038 1587
Size: 43 132 863 549
Fragments arranged by size:
863 549 132 43
StyI C'CwwG_G
Cuts at: 0 135 1587
Size: 135 1452
TatI w'GTAC_w
Cuts at: 0 1470 1587
Size: 1470 117
XhoI C'TCGA_G
Cuts at: 0 43 1587
Size: 43 1544
XmnI GAAnn'nnTTC
Cuts at: 0 604 641 772 940 1229 1587
Size: 604 37 131 168 289 358
Fragments arranged by size:
604 358 289 168 131 37
Enzymes that do cut:
AccI AcuI AflIII AleI ApaLI ApoI AvaI BamHI
BanI BbeI BbsI BcgI BfrBI BglII Bme1580I BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BstBI BstYI BtgI BtsI
CspCI DraI DraIII EaeI EarI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HindIII KasI MscI MslI
NarI NcoI NsiI NspI PciI PmlI PspXI PstI
SapI SfcI SfoI SmlI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BanII BbvCI BciVI BclI BglI BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaBI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspAI
HincII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XmaI ZraI