Restriction map for
pF1KSDA1200
(Linear) (Six-base) MAPSORT of: KIAA1200.seq Check: 3197 from: 1 to: 4092
KIAA1200.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 2374 4092
Size: 465 1909 1718
Fragments arranged by size:
1909 1718 465
AccI GT'mk_AC
Cuts at: 0 35 1391 1415 4092
Size: 35 1356 24 2677
Fragments arranged by size:
2677 1356 35 24
AclI AA'CG_TT
Cuts at: 0 3823 4084 4092
Size: 3823 261 8
Fragments arranged by size:
3823 261 8
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 560 652 710 807 1447 2032 2973
Size: 247 313 92 58 97 640 585 941
Cuts at: 2973 3797 4092
Size: 824 295
Fragments arranged by size:
941 824 640 585 313 295 247 97
92 58
AflIII A'CryG_T
Cuts at: 0 3255 4092
Size: 3255 837
AleI CACnn'nnGTG
Cuts at: 0 2290 4092
Size: 2290 1802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3016 4092
Size: 3016 1076
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3317 3349 4092
Size: 3317 32 743
Fragments arranged by size:
3317 743 32
AlwNI CAG_nnn'CTG
Cuts at: 0 135 147 444 465 630 1669 2007 2380
Size: 135 12 297 21 165 1039 338 373
Cuts at: 2380 3075 3466 4092
Size: 695 391 626
Fragments arranged by size:
1039 695 626 391 373 338 297 165
135 21 12
ApaI G_GGCC'C
Cuts at: 0 3552 4092
Size: 3552 540
ApaLI G'TGCA_C
Cuts at: 0 3001 3790 4092
Size: 3001 789 302
Fragments arranged by size:
3001 789 302
AvaI C'yCGr_G
Cuts at: 0 1097 1109 1568 1836 2671 3141 4092
Size: 1097 12 459 268 835 470 951
Fragments arranged by size:
1097 951 835 470 459 268 12
BamHI G'GATC_C
Cuts at: 0 481 1988 4092
Size: 481 1507 2104
Fragments arranged by size:
2104 1507 481
BanI G'GyrC_C
Cuts at: 0 21 537 661 955 989 2053 2335 2477
Size: 21 516 124 294 34 1064 282 142
Cuts at: 2477 3569 4092
Size: 1092 523
Fragments arranged by size:
1092 1064 523 516 294 282 142 124
34 21
BanII G_rGCy'C
Cuts at: 0 305 790 951 1180 1609 3552 3883 4092
Size: 305 485 161 229 429 1943 331 209
Fragments arranged by size:
1943 485 429 331 305 229 209 161
BbeI G_GCGC'C
Cuts at: 0 25 4092
Size: 25 4067
BbsI GAAGACnn'nnnn_
Cuts at: 0 1552 1783 3704 4092
Size: 1552 231 1921 388
Fragments arranged by size:
1921 1552 388 231
BbvCI CC'TCA_GC
Cuts at: 0 2792 3565 4092
Size: 2792 773 527
Fragments arranged by size:
2792 773 527
BciVI GTATCCnnnnn_n'
Cuts at: 0 2346 4092
Size: 2346 1746
BfrBI ATG'CAT
Cuts at: 0 806 4092
Size: 806 3286
BglI GCCn_nnn'nGGC
Cuts at: 0 3418 3470 4092
Size: 3418 52 622
Fragments arranged by size:
3418 622 52
BglII A'GATC_T
Cuts at: 0 2687 3182 3862 4092
Size: 2687 495 680 230
Fragments arranged by size:
2687 680 495 230
BlpI GC'TnA_GC
Cuts at: 0 323 1196 3491 3695 4092
Size: 323 873 2295 204 397
Fragments arranged by size:
2295 873 397 323 204
Bme1580I G_kGCm'C
Cuts at: 0 542 666 1022 2410 3005 3552 3794 4092
Size: 542 124 356 1388 595 547 242 298
Fragments arranged by size:
1388 595 547 542 356 298 242 124
BmgBI CAC'GTC
Cuts at: 0 1781 4092
Size: 1781 2311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 895 1074 4092
Size: 895 179 3018
Fragments arranged by size:
3018 895 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 172 349 1111 1222 1756 1984 1999
Size: 157 15 177 762 111 534 228 15
Cuts at: 1999 2018 2273 2493 3052 3175 3487 3488 4092
Size: 19 255 220 559 123 312 1 604
Fragments arranged by size:
762 604 559 534 312 255 228 220
177 157 123 111 19 15 15 1
Bpu10I CC'TnA_GC
Cuts at: 0 1085 1670 2792 3565 4092
Size: 1085 585 1122 773 527
Fragments arranged by size:
1122 1085 773 585 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 445 1844 2545 3421 4092
Size: 394 51 1399 701 876 671
Fragments arranged by size:
1399 876 701 671 394 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 4092
Size: 175 3917
BsaAI yAC'GTr
Cuts at: 0 2357 4092
Size: 2357 1735
BsaHI Gr'CG_yC
Cuts at: 0 22 1586 1637 3758 4092
Size: 22 1564 51 2121 334
Fragments arranged by size:
2121 1564 334 51 22
BsaWI w'CCGG_w
Cuts at: 0 77 257 1850 3914 4092
Size: 77 180 1593 2064 178
Fragments arranged by size:
2064 1593 180 178 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 598 628 2016 2046 3317 3347 4092
Size: 598 30 1388 30 1271 30 745
Fragments arranged by size:
1388 1271 745 598 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1153 1521 1996 2317 2752 4092
Size: 1153 368 475 321 435 1340
Fragments arranged by size:
1340 1153 475 435 368 321
BseYI C'CCAG_C
Cuts at: 0 1149 1215 1713 1969 3704 4092
Size: 1149 66 498 256 1735 388
Fragments arranged by size:
1735 1149 498 388 256 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1228 2038 2268 3211 4092
Size: 238 990 810 230 943 881
Fragments arranged by size:
990 943 881 810 238 230
BsiEI CG_ry'CG
Cuts at: 0 1961 4092
Size: 1961 2131
BsiHKAI G_wGCw'C
Cuts at: 0 305 1609 1676 2312 2778 3005 3161 3794
Size: 305 1304 67 636 466 227 156 633
Cuts at: 3794 3883 4092
Size: 89 209
Fragments arranged by size:
1304 636 633 466 305 227 209 156
89 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1593 3307 4092
Size: 1593 1714 785
Fragments arranged by size:
1714 1593 785
Bsp1286I G_dGCh'C
Cuts at: 0 305 542 666 790 951 1022 1180 1609
Size: 305 237 124 124 161 71 158 429
Cuts at: 1609 1676 2312 2410 2778 3005 3161 3552 3794
Size: 67 636 98 368 227 156 391 242
Cuts at: 3794 3883 4092
Size: 89 209
Fragments arranged by size:
636 429 391 368 305 242 237 227
209 161 158 156 124 124 98 89
71 67
BspEI T'CCGG_A
Cuts at: 0 1850 3914 4092
Size: 1850 2064 178
Fragments arranged by size:
2064 1850 178
BspHI T'CATG_A
Cuts at: 0 2702 4092
Size: 2702 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1760 2374 2643 3498 4092
Size: 465 1295 614 269 855 594
Fragments arranged by size:
1295 855 614 594 465 269
BsrBI CCG'CTC
Cuts at: 0 2469 2895 2916 4092
Size: 2469 426 21 1176
Fragments arranged by size:
2469 1176 426 21
BsrDI GCAATG_nn'
Cuts at: 0 3604 3972 4092
Size: 3604 368 120
Fragments arranged by size:
3604 368 120
BsrFI r'CCGG_y
Cuts at: 0 24 2598 4092
Size: 24 2574 1494
Fragments arranged by size:
2574 1494 24
BsrGI T'GTAC_A
Cuts at: 0 3272 4092
Size: 3272 820
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 1680 2484 3165 4092
Size: 147 1533 804 681 927
Fragments arranged by size:
1533 927 804 681 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 610 1075 1977 3031 4092
Size: 610 465 902 1054 1061
Fragments arranged by size:
1061 1054 902 610 465
BstYI r'GATC_y
Cuts at: 0 481 1271 1872 1988 2454 2687 2841 3182
Size: 481 790 601 116 466 233 154 341
Cuts at: 3182 3862 4092
Size: 680 230
Fragments arranged by size:
790 680 601 481 466 341 233 230
154 116
BtgI C'CryG_G
Cuts at: 0 961 1014 3050 3650 4092
Size: 961 53 2036 600 442
Fragments arranged by size:
2036 961 600 442 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2915 4092
Size: 2915 1177
BtsI GCAGTG_nn'
Cuts at: 0 2280 2400 2408 2647 2755 2894 3159 4092
Size: 2280 120 8 239 108 139 265 933
Fragments arranged by size:
2280 933 265 239 139 120 108 8
ClaI AT'CG_AT
Cuts at: 0 2220 4092
Size: 2220 1872
EaeI y'GGCC_r
Cuts at: 0 26 980 1026 1283 1958 2097 2859 3653
Size: 26 954 46 257 675 139 762 794
Cuts at: 3653 3956 4092
Size: 303 136
Fragments arranged by size:
954 794 762 675 303 257 139 136
46 26
EagI C'GGCC_G
Cuts at: 0 1958 4092
Size: 1958 2134
EarI CTCTTCn'nnn_
Cuts at: 0 197 774 1780 2572 2590 3051 3130 3816
Size: 197 577 1006 792 18 461 79 686
Cuts at: 3816 4092
Size: 276
Fragments arranged by size:
1006 792 686 577 461 276 197 79
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 4092
Size: 73 4019
EcoICRI GAG'CTC
Cuts at: 0 303 1607 3881 4092
Size: 303 1304 2274 211
Fragments arranged by size:
2274 1304 303 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 172 247 349 560 652 710 807
Size: 157 15 75 102 211 92 58 97
Cuts at: 807 1111 1222 1447 1756 1984 1999 2018 2032
Size: 304 111 225 309 228 15 19 14
Cuts at: 2032 2273 2493 2973 3052 3175 3487 3488 3797
Size: 241 220 480 79 123 312 1 309
Cuts at: 3797 4092
Size: 295
Fragments arranged by size:
480 312 309 309 304 295 241 228
225 220 211 157 123 111 102 97
92 79 75 58 19 15 15 14
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 656 900 937 1807 1978 2011 3144 4092
Size: 656 244 37 870 171 33 1133 948
Fragments arranged by size:
1133 948 870 656 244 171 37 33
EcoO109I rG'GnC_Cy
Cuts at: 0 1106 1185 2556 3150 3463 3510 3548 3549
Size: 1106 79 1371 594 313 47 38 1
Cuts at: 3549 4092
Size: 543
Fragments arranged by size:
1371 1106 594 543 313 79 47 38
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3927 4092
Size: 3927 165
FspI TGC'GCA
Cuts at: 0 2201 4092
Size: 2201 1891
FspAI rTGC'GCAy
Cuts at: 0 2201 4092
Size: 2201 1891
HaeII r_GCGC'y
Cuts at: 0 25 465 1794 3537 4092
Size: 25 440 1329 1743 555
Fragments arranged by size:
1743 1329 555 440 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 598 630 1078 1110 1459 1483
Size: 420 32 146 32 448 32 349 24
Cuts at: 1483 1491 1515 1627 1659 1864 1896 3124 3156
Size: 8 24 112 32 205 32 1228 32
Cuts at: 3156 3181 3213 3865 3897 3909 3941 4092
Size: 25 32 652 32 12 32 151
Fragments arranged by size:
1228 652 448 420 349 205 151 146
112 32 32 32 32 32 32 32
32 32 25 24 24 12 8
HincII GTy'rAC
Cuts at: 0 3587 4092
Size: 3587 505
HindIII A'AGCT_T
Cuts at: 0 421 811 4092
Size: 421 390 3281
Fragments arranged by size:
3281 421 390
KasI G'GCGC_C
Cuts at: 0 21 4092
Size: 21 4071
MluI A'CGCG_T
Cuts at: 0 3255 4092
Size: 3255 837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2853 4092
Size: 2853 1239
MscI TGG'CCA
Cuts at: 0 982 1285 2099 2861 3655 3958 4092
Size: 982 303 814 762 794 303 134
Fragments arranged by size:
982 814 794 762 303 303 134
MslI CAynn'nnrTG
Cuts at: 0 2290 2593 2941 2998 3853 4092
Size: 2290 303 348 57 855 239
Fragments arranged by size:
2290 855 348 303 239 57
MspA1I CmG'CkG
Cuts at: 0 327 640 963 1242 1553 1886 2717 2883
Size: 327 313 323 279 311 333 831 166
Cuts at: 2883 3072 3556 3564 3708 4023 4092
Size: 189 484 8 144 315 69
Fragments arranged by size:
831 484 333 327 323 315 313 311
279 189 166 144 69 8
NaeI GCC'GGC
Cuts at: 0 26 4092
Size: 26 4066
NarI GG'CG_CC
Cuts at: 0 22 4092
Size: 22 4070
NcoI C'CATG_G
Cuts at: 0 1014 4092
Size: 1014 3078
NgoMIV G'CCGG_C
Cuts at: 0 24 4092
Size: 24 4068
NsiI A_TGCA'T
Cuts at: 0 808 4092
Size: 808 3284
NspI r_CATG'y
Cuts at: 0 1299 1337 1458 4092
Size: 1299 38 121 2634
Fragments arranged by size:
2634 1299 121 38
PasI CC'CwG_GG
Cuts at: 0 624 883 1369 2175 4092
Size: 624 259 486 806 1917
Fragments arranged by size:
1917 806 624 486 259
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 3466 3596 3662 4092
Size: 597 2869 130 66 430
Fragments arranged by size:
2869 597 430 130 66
PfoI T'CCnGG_A
Cuts at: 0 1540 3767 4092
Size: 1540 2227 325
Fragments arranged by size:
2227 1540 325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3317 3349 4092
Size: 3317 32 743
Fragments arranged by size:
3317 743 32
PpuMI rG'GwC_Cy
Cuts at: 0 1106 3150 3510 4092
Size: 1106 2044 360 582
Fragments arranged by size:
2044 1106 582 360
PshAI GACnn'nnGTC
Cuts at: 0 3256 4092
Size: 3256 836
PspOMI G'GGCC_C
Cuts at: 0 3548 4092
Size: 3548 544
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3271 3303 4092
Size: 3271 32 789
Fragments arranged by size:
3271 789 32
PstI C_TGCA'G
Cuts at: 0 558 671 1498 1685 2385 2654 2681 3170
Size: 558 113 827 187 700 269 27 489
Cuts at: 3170 3838 4092
Size: 668 254
Fragments arranged by size:
827 700 668 558 489 269 254 187
113 27
PvuII CAG'CTG
Cuts at: 0 327 640 2717 3564 3708 4023 4092
Size: 327 313 2077 847 144 315 69
Fragments arranged by size:
2077 847 327 315 313 144 69
RsrII CG'GwC_CG
Cuts at: 0 2906 3318 4092
Size: 2906 412 774
Fragments arranged by size:
2906 774 412
SacI G_AGCT'C
Cuts at: 0 305 1609 3883 4092
Size: 305 1304 2274 209
Fragments arranged by size:
2274 1304 305 209
SacII CC_GC'GG
Cuts at: 0 964 4092
Size: 964 3128
SapI GCTCTTCn'nnn_
Cuts at: 0 2572 2590 3051 4092
Size: 2572 18 461 1041
Fragments arranged by size:
2572 1041 461 18
SbfI CC_TGCA'GG
Cuts at: 0 3170 4092
Size: 3170 922
ScaI AGT'ACT
Cuts at: 0 1997 2737 4092
Size: 1997 740 1355
Fragments arranged by size:
1997 1355 740
SfcI C'TryA_G
Cuts at: 0 88 554 667 1494 1681 2381 2493 2650
Size: 88 466 113 827 187 700 112 157
Cuts at: 2650 2677 3166 3537 3834 4092
Size: 27 489 371 297 258
Fragments arranged by size:
827 700 489 466 371 297 258 187
157 113 112 88 27
SfoI GGC'GCC
Cuts at: 0 23 4092
Size: 23 4069
SmaI CCC'GGG
Cuts at: 0 1111 1570 4092
Size: 1111 459 2522
Fragments arranged by size:
2522 1111 459
SmlI C'TyrA_G
Cuts at: 0 10 409 424 1859 2560 3400 4092
Size: 10 399 15 1435 701 840 692
Fragments arranged by size:
1435 840 701 692 399 15 10
SphI G_CATG'C
Cuts at: 0 1299 1337 4092
Size: 1299 38 2755
Fragments arranged by size:
2755 1299 38
StuI AGG'CCT
Cuts at: 0 1054 2542 4092
Size: 1054 1488 1550
Fragments arranged by size:
1550 1488 1054
StyI C'CwwG_G
Cuts at: 0 704 1014 1188 2084 2105 2399 3485 3716
Size: 704 310 174 896 21 294 1086 231
Cuts at: 3716 4073 4092
Size: 357 19
Fragments arranged by size:
1086 896 704 357 310 294 231 174
21 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 390 1512 1983 3335 4092
Size: 181 209 1122 471 1352 757
Fragments arranged by size:
1352 1122 757 471 209 181
TatI w'GTAC_w
Cuts at: 0 1823 1995 2735 3272 3287 4092
Size: 1823 172 740 537 15 805
Fragments arranged by size:
1823 805 740 537 172 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2016 2048 3120 3152 4092
Size: 2016 32 1072 32 940
Fragments arranged by size:
2016 1072 940 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1012 1021 1710 3430 3951 4092
Size: 1012 9 689 1720 521 141
Fragments arranged by size:
1720 1012 689 521 141 9
XmaI C'CCGG_G
Cuts at: 0 1109 1568 4092
Size: 1109 459 2524
Fragments arranged by size:
2524 1109 459
XmnI GAAnn'nnTTC
Cuts at: 0 506 2137 4092
Size: 506 1631 1955
Fragments arranged by size:
1955 1631 506
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuII RsrII SacI SacII SapI
SbfI ScaI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BcgI BclI BmrI BmtI
BsaBI BsiWI BsmI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I CspCI DraI DraIII DrdI EcoRI EcoRV FseI
HpaI KpnI MfeI NdeI NheI NotI NruI PacI
PciI PmeI PmlI PsiI PspXI PvuI SalI SanDI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI