Restriction map for
pF1KSDA1201
(Linear) (Six-base) MAPSORT of: KIAA1201.seq Check: 5514 from: 1 to: 2094
KIAA1201.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1598 1736 2094
Size: 1598 138 358
Fragments arranged by size:
1598 358 138
AclI AA'CG_TT
Cuts at: 0 1420 2094
Size: 1420 674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 238 1011 2014 2094
Size: 156 82 773 1003 80
Fragments arranged by size:
1003 773 156 82 80
AflIII A'CryG_T
Cuts at: 0 1668 2057 2094
Size: 1668 389 37
Fragments arranged by size:
1668 389 37
AhdI GACnn_n'nnGTC
Cuts at: 0 1369 2058 2094
Size: 1369 689 36
Fragments arranged by size:
1369 689 36
AleI CACnn'nnGTG
Cuts at: 0 1342 2094
Size: 1342 752
ApaI G_GGCC'C
Cuts at: 0 444 1917 2094
Size: 444 1473 177
Fragments arranged by size:
1473 444 177
ApaLI G'TGCA_C
Cuts at: 0 230 2094
Size: 230 1864
ApoI r'AATT_y
Cuts at: 0 105 2094
Size: 105 1989
AvaI C'yCGr_G
Cuts at: 0 443 2094
Size: 443 1651
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1924 1957 2094
Size: 1924 33 137
Fragments arranged by size:
1924 137 33
BanII G_rGCy'C
Cuts at: 0 16 444 521 836 935 1624 1917 1958
Size: 16 428 77 315 99 689 293 41
Cuts at: 1958 2094
Size: 136
Fragments arranged by size:
689 428 315 293 136 99 77 41
16
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 1011 2094
Size: 173 838 1083
Fragments arranged by size:
1083 838 173
BciVI GTATCCnnnnn_n'
Cuts at: 0 610 2094
Size: 610 1484
BglI GCCn_nnn'nGGC
Cuts at: 0 95 2094
Size: 95 1999
Bme1580I G_kGCm'C
Cuts at: 0 234 444 590 1298 1917 2094
Size: 234 210 146 708 619 177
Fragments arranged by size:
708 619 234 210 177 146
BmgBI CAC'GTC
Cuts at: 0 1293 2060 2094
Size: 1293 767 34
Fragments arranged by size:
1293 767 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 1890 2094
Size: 75 1815 204
Fragments arranged by size:
1815 204 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 265 2094
Size: 30 235 1829
Fragments arranged by size:
1829 235 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1461 1471 2094
Size: 811 650 10 623
Fragments arranged by size:
811 650 623 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 1377 2094
Size: 676 701 717
Fragments arranged by size:
717 701 676
BsaAI yAC'GTr
Cuts at: 0 585 1008 1263 2094
Size: 585 423 255 831
Fragments arranged by size:
831 585 423 255
BsaWI w'CCGG_w
Cuts at: 0 48 2094
Size: 48 2046
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1560 1590 2094
Size: 1560 30 504
Fragments arranged by size:
1560 504 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 66 1042 1150 1581 2094
Size: 15 51 976 108 431 513
Fragments arranged by size:
976 513 431 108 51 15
BseYI C'CCAG_C
Cuts at: 0 311 1916 2094
Size: 311 1605 178
Fragments arranged by size:
1605 311 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 2094
Size: 82 2012
BsiEI CG_ry'CG
Cuts at: 0 90 2094
Size: 90 2004
BsiHKAI G_wGCw'C
Cuts at: 0 234 521 836 935 1501 1958 2094
Size: 234 287 315 99 566 457 136
Fragments arranged by size:
566 457 315 287 234 136 99
BsmI GAATG_Cn'
Cuts at: 0 489 2094
Size: 489 1605
BsmBI CGTCTCn'nnnn_
Cuts at: 0 774 806 2094
Size: 774 32 1288
Fragments arranged by size:
1288 774 32
Bsp1286I G_dGCh'C
Cuts at: 0 16 234 444 521 590 836 935 1298
Size: 16 218 210 77 69 246 99 363
Cuts at: 1298 1501 1624 1917 1958 2094
Size: 203 123 293 41 136
Fragments arranged by size:
363 293 246 218 210 203 136 123
99 77 69 41 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1598 1666 1736 2094
Size: 1598 68 70 358
Fragments arranged by size:
1598 358 70 68
BsrBI CCG'CTC
Cuts at: 0 95 2094
Size: 95 1999
BsrFI r'CCGG_y
Cuts at: 0 998 2094
Size: 998 1096
BsrGI T'GTAC_A
Cuts at: 0 1232 2094
Size: 1232 862
BstAPI GCAn_nnn'nTGC
Cuts at: 0 60 2094
Size: 60 2034
BstEII G'GTnAC_C
Cuts at: 0 1626 1889 2094
Size: 1626 263 205
Fragments arranged by size:
1626 263 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 544 1191 2094
Size: 544 647 903
Fragments arranged by size:
903 647 544
BstYI r'GATC_y
Cuts at: 0 284 629 2094
Size: 284 345 1465
Fragments arranged by size:
1465 345 284
BtgI C'CryG_G
Cuts at: 0 1123 2094
Size: 1123 971
BtsI GCAGTG_nn'
Cuts at: 0 762 1856 2094
Size: 762 1094 238
Fragments arranged by size:
1094 762 238
DraIII CAC_nnn'GTG
Cuts at: 0 1293 2094
Size: 1293 801
EaeI y'GGCC_r
Cuts at: 0 87 996 1484 2094
Size: 87 909 488 610
Fragments arranged by size:
909 610 488 87
EagI C'GGCC_G
Cuts at: 0 87 2094
Size: 87 2007
EarI CTCTTCn'nnn_
Cuts at: 0 620 635 791 948 1060 1604 1819 2094
Size: 620 15 156 157 112 544 215 275
Fragments arranged by size:
620 544 275 215 157 156 112 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1453 2094
Size: 1453 641
EcoICRI GAG'CTC
Cuts at: 0 519 834 933 1956 2094
Size: 519 315 99 1023 138
Fragments arranged by size:
1023 519 315 138 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 238 811 1011 1461 1471 2014 2094
Size: 156 82 573 200 450 10 543 80
Fragments arranged by size:
573 543 450 200 156 82 80 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 826 1609 2094
Size: 826 783 485
Fragments arranged by size:
826 783 485
EcoO109I rG'GnC_Cy
Cuts at: 0 440 762 818 987 2045 2094
Size: 440 322 56 169 1058 49
Fragments arranged by size:
1058 440 322 169 56 49
EcoRI G'AATT_C
Cuts at: 0 105 2094
Size: 105 1989
EcoRV GAT'ATC
Cuts at: 0 1110 2094
Size: 1110 984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 2094
Size: 441 1653
HaeII r_GCGC'y
Cuts at: 0 210 805 1101 2094
Size: 210 595 296 993
Fragments arranged by size:
993 595 296 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 254 286 649 681 861 893
Size: 10 32 212 32 363 32 180 32
Cuts at: 893 1355 1387 1560 1592 2044 2076 2094
Size: 462 32 173 32 452 32 18
Fragments arranged by size:
462 452 363 212 180 173 32 32
32 32 32 32 32 18 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1814 2042 2094
Size: 971 843 228 52
Fragments arranged by size:
971 843 228 52
MscI TGG'CCA
Cuts at: 0 1486 2094
Size: 1486 608
MslI CAynn'nnrTG
Cuts at: 0 1342 2094
Size: 1342 752
MspA1I CmG'CkG
Cuts at: 0 71 311 708 1077 1095 1763 2094
Size: 71 240 397 369 18 668 331
Fragments arranged by size:
668 397 369 331 240 71 18
NaeI GCC'GGC
Cuts at: 0 1000 2094
Size: 1000 1094
NcoI C'CATG_G
Cuts at: 0 1123 2094
Size: 1123 971
NgoMIV G'CCGG_C
Cuts at: 0 998 2094
Size: 998 1096
NspI r_CATG'y
Cuts at: 0 1672 2094
Size: 1672 422
PasI CC'CwG_GG
Cuts at: 0 1403 2094
Size: 1403 691
PciI A'CATG_T
Cuts at: 0 1668 2094
Size: 1668 426
PfoI T'CCnGG_A
Cuts at: 0 1700 2094
Size: 1700 394
PpuMI rG'GwC_Cy
Cuts at: 0 818 987 2045 2094
Size: 818 169 1058 49
Fragments arranged by size:
1058 818 169 49
PspOMI G'GGCC_C
Cuts at: 0 440 1913 2094
Size: 440 1473 181
Fragments arranged by size:
1473 440 181
PstI C_TGCA'G
Cuts at: 0 1733 2094
Size: 1733 361
PvuII CAG'CTG
Cuts at: 0 71 708 1763 2094
Size: 71 637 1055 331
Fragments arranged by size:
1055 637 331 71
SacI G_AGCT'C
Cuts at: 0 521 836 935 1958 2094
Size: 521 315 99 1023 136
Fragments arranged by size:
1023 521 315 136 99
SanDI GG'GwC_CC
Cuts at: 0 818 2094
Size: 818 1276
SapI GCTCTTCn'nnn_
Cuts at: 0 1819 2094
Size: 1819 275
SfcI C'TryA_G
Cuts at: 0 294 1729 2094
Size: 294 1435 365
Fragments arranged by size:
1435 365 294
SmaI CCC'GGG
Cuts at: 0 445 2094
Size: 445 1649
StuI AGG'CCT
Cuts at: 0 976 2094
Size: 976 1118
StyI C'CwwG_G
Cuts at: 0 512 1123 2094
Size: 512 611 971
Fragments arranged by size:
971 611 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1300 2094
Size: 1300 794
TatI w'GTAC_w
Cuts at: 0 1232 2094
Size: 1232 862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1749 2094
Size: 1749 345
XmaI C'CCGG_G
Cuts at: 0 443 2094
Size: 443 1651
XmnI GAAnn'nnTTC
Cuts at: 0 1420 1435 2094
Size: 1420 15 659
Fragments arranged by size:
1420 659 15
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI ApaI ApaLI
ApoI AvaI BaeI BanII BbsI BciVI BglI Bme1580I
BmgBI BmrI BplI BpmI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI SmaI StuI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AlfI AloI
AlwNI AscI AseI AsiSI AvrII BamHI BanI BbeI
BbvCI BcgI BclI BfrBI BglII BlpI BmtI Bpu10I
BpuEI BsaBI BsaHI BsiWI BspEI BspHI BsrDI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
ZraI